针刺对大鼠肾上腺素能的影响

临床实践表明,针灸对多种慢性、耗损性疾病有不同程度疗效,为探索其机理,本实验做了如下动物实验观察。 1.大鼠每日腹腔注射氢化可的松3毫克/公斤,连续9天,造成动物机体耗损,动物呈现一系列肾上腺皮质机能减退状态:毛松,少动,弓背,肾上腺萎缩(重量明显减轻),血浆糖皮质激素含量明显降低等。对这种耗损动物给以电针治疗(双“肾俞”穴,电针频率15次/秒,强度以动物耐受为度,间日电针一次,每次15分钟)经9次电针后,大鼠肾上腺湿重,血浆糖皮质激素含量都显著高于模型对照组。这一结果提示,针刺可以促进萎缩肾上腺修复,改善其分泌活动。 2.由上述实验结果表明,在动物血浆糖皮质激素处于低水平时,针刺对肾上腺皮质分泌活动具有激活作用,而当动物体内保持较高和平的列源性糖皮质激素时,针刺的作用又如何呢;对中枢五羟色胺系统是否参与垂体-肾上腺系统的调节的有关报道不鲜,那么中枢五羟色胺系统是否参与针刺的调正作用,本实验就如上问题做了初步探索。大鼠一次腹腔注射大剂量长较外源性糖皮质激素地塞米松(6.8毫克/公斤体重)4小时后,动物血浆皮质酮含量显著降低,表明肾上腺皮质分泌活动处于反馈性抑制状态。对这种地塞米松动物电针15分钟(方法同实验1)后,动物血浆皮质酮水平显著升高,它表明针刺可以显著对抗外源性激素对肾上腺皮质系统的反馈性抑制。在本实验中还观察到,注射地塞米松后,大鼠间脑5-HT,5-HIAA含量明显降低,地塞米松对5-HT的合成、代谢具有抑制作用。地塞米松动物电针15分钟后,其间脑5-HT,5-HTIAA水平明显高于地塞米松对照组,但仍显著低于盐水加电针组,针刺对地塞米松动物中枢5-HT系统功能活动具有激活作用,但激活程度与血糖皮质激素水平有关,中枢5-HT系统可能参与针刺对垂体-肾上腺皮质系统的调整作用。     

艾灸治疗关节痛的疗效与神经组织内P物质和脑啡肽的关系

我们用福氏佐剂诱发的关节炎大鼠作为病理模型，观察艾灸对患关节的压痛闽，局部皮肤温度，足容积，以及坐骨神经内的和腰髓内的P-物质和亮-脑啡肽的影响，试图探索艾灸治疗关节痛的作用机理。     

THE RELATIONSHIP BETWEEN cAMP AND cGMP IN REGIONS OF RAT BRAIN AND ACUPUNCTURE ANALGESIA

In this experiment,animals were sacrificedby total immersion in liquid nitrogen to fix thebrain tissue,cAMP and cGMP in brain areaswere determined by radioimmunoassay.Wecorrelated the levels of cAMP and cGMP in thebrain with acupuncture analgesia.Our resultsshow that acupuncture analgesia led to a rise ofcGMP in the rat diencephalon and lower brainstem,while the levels of cAMP in the rat brainareas were unchanged after acupuncture.     

艾灸治疗关节痛的疗效与神经组织内p-物质和脑啡肽的关系

艾灸对福氏佐剂引起的关节炎大鼠的关节痛有显著疗效。艾灸也有助于患鼠坐骨神经内异常P-物质含量的恢复;并能使腰髓内亮-脑啡肽和P-物质保持较高的水平。结果表明,艾灸能加强脊髓内脑啡肽系统的功能,并可能激活下行的P-物质能神经元,它们在调制一级传入神经元的功能方面起重要的作用,因此艾灸的上述作用可能与其治疗关节痛的疗效有关。     

中枢5-羟色胺能系统可能参与生大黄对大鼠拘束水浸应激性胃溃疡的抑制作用

大黄是常用中药,近年来,临床上用单味生大黄或炮制品治疗上消化道出血获得较好疗效,实验研究也证明生大黄和酒炖大黄对大鼠拘束水浸应激性胃溃疡有明显的抑制作用。为探讨其机制,我们观察了生大黄和酒     

大鼠脑内P物质在针刺镇痛中的作用及其与脑啡肽的关系

实验观察了大鼠脑室注射SP拮抗剂[D—Arg^1，D-Phe^5，D-Trp^7·8，Leu^11)-SP(简称DADPDTL)对电针镇痛效应的影响，电针刺激后大鼠脑内SP含量的变化以及脑室或腹腔注射纳洛酮后对电针引起的SP含量变化是否有影响。     

EFFECT OF ACUPUNCTURE ON THE ACTIVITIES OF PHOSPHODIESTERASESINRATBRAIN

The relationship between the activities of pho-sphodiesterases(PDEs)in brain regions and acupunc-ture analgesia were studied.Results showed that inacupuncture analgesia group,the activity of cAMP-PDE in the brain stem,diencephalon and striatumwas the same as in acupuncture non-analgesia,re-strained control,and normal control groups.But theactivity of cGMP-PDE in the brain stem of the acu-puncture analgesia group was decreased significant-ly(compared with that of the restrained controlgroup,P<0.01,and also compared with that of thenormal control and the acupuncture non-analgesiagroups P<0.05).The study suggested the possibilityof elevation of cGMP level in the brain stem as dueto the decreased activity of cGMP-PDE in this re-gion.     

THE EFFECT OF ELECTROACUPUNCTURE ON THE FUNCTION OF SYMPATHETO-ADRENAL MEDULLA

The contents of plasma cAMP in rats fol-lowing acupuncture in different conditions weredetermined by radioimmunoassay.The levels ofplasma cAMP in the normal group,the groupof rats with sympathetic nerve endings damag-ed by 6-hydroxydopamine,and the group of ratswith bilateral adrenalectomy were all signifi-cantly elevated by electroacupuncture,especial-ly in the normal groug,though eletroacupunc-ture did not raise the levels of plasma cAMPin rats with bilateral adrenalectomy and da-maged sympathoadrenergie nerve endings.Re-suits show that the changes in plasma cAMPproduced by electroacupuncture are related tosympathetoadrenal medullar function.In otherwords,electroacupuncture activates both thesympathetic nerve and the adrenal medulla,where catecholamine is released to regulatethe physiological functions of the organismthrough cyclic AMP.     

针刺对血浆环核苷酸的影响和针刺镇痛与血浆环核苷酸水平的关系

针刺在临床上能治疗和缓解多种疾患,其主要特点是激发或调整机体内在的生理功能,使之正常化,即中医所说的“激发精气”、“疏导气血”、“调和阴阳”。针刺疗法的这种特点表明,不同的机体状态,对针刺