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Objective To explore the influence of Helicobacter pylori (Hp) on the proliferation, apoptosis and expression of p27kipl protein of gastric epithelial cells in vitro. Methods SC, C-7901 cell pro-liferation was examined by flow cytometry after incubation with different concentration of NCTC11637. The effect of NCTC11637 on cell apoptosis was also evaluated by flow cytometryo And the expression of p27kipl of gastric epithelial cells was detected by immunohistochemical analysis and in situ hybridization, respectively. Results Cell proliferation was enhanced when co-incubated with the Hp in low concentration (3.4 x 104 to 1.9 x 105 CFU/ml) but inhibited in higher concentration (≥4.8×106CFU/ml). However, cell apoptosis was increase when co-incubated with the Hp in high concentration (≥9.6 ×105 CFU/ml) showing concen-tration dependent picture. In addition, the co-incubation of SGC-7901 with Hp led to decrease of the expres-sion of p27kipl protein but not mRNA in a Hp concentration dependent way. Conclusion Hp could effect the gastric epithelial cells apoptosis and proliferation directly and influence the expression of p27kips protein which might facilitate gastric carcinoma. 相似文献
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胃黏膜瘦素的研究进展 总被引:1,自引:0,他引:1
瘦素(1eptin)是肥胖基因编码的分泌型蛋白,在代谢平衡中主要通过作用于下丘脑特异性受体调节食物摄入能量平衡。起初认为白色脂肪组织是瘦素的唯一来源。进一步研究证实,瘦素在胎盘、骨骼肌、乳腺上皮细胞等多种组织器官中均有表达。瘦素受体不仅存在于与代谢有关的下丘脑、骨骼肌,还广泛存在于全身各组织器官,如脂肪组 相似文献
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胃疾病中幽门螺杆菌细胞毒相关基因A和E与白细胞介素-8相关性研究 总被引:1,自引:0,他引:1
幽门螺杆菌 (Helicobacterpylori,Hp)是一种革兰染色阴性 ,呈弧形、S型或海鸥状的微需氧菌 ,专性定植于人胃黏膜上皮表面。Hp普遍被认为是慢性胃炎、胃溃疡、胃癌及胃黏膜相关淋巴组织淋巴瘤的致病因子 ,但其确切的致病机制尚未阐明。目前多数学者认为 ,Hp感染所致的胃组织损伤程度及临床症状的严重程度同Hp菌株的毒力相关。细胞毒相关基因A(cytotoxinassociationgeneA ,CagA)及Cag毒力岛 (Cagpathogenicityisland ,CagPAI)是目前Hp致病机制研究的热点。经典的观点认为 ,CagA基因阳性菌株诱导胃黏膜上皮细胞分泌白细胞介素 (IL) 8… 相似文献
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Objective To explore the influence of Helicobacter pylori (Hp) on the proliferation, apoptosis and expression of p27kipl protein of gastric epithelial cells in vitro. Methods SC, C-7901 cell pro-liferation was examined by flow cytometry after incubation with different concentration of NCTC11637. The effect of NCTC11637 on cell apoptosis was also evaluated by flow cytometryo And the expression of p27kipl of gastric epithelial cells was detected by immunohistochemical analysis and in situ hybridization, respectively. Results Cell proliferation was enhanced when co-incubated with the Hp in low concentration (3.4 x 104 to 1.9 x 105 CFU/ml) but inhibited in higher concentration (≥4.8×106CFU/ml). However, cell apoptosis was increase when co-incubated with the Hp in high concentration (≥9.6 ×105 CFU/ml) showing concen-tration dependent picture. In addition, the co-incubation of SGC-7901 with Hp led to decrease of the expres-sion of p27kipl protein but not mRNA in a Hp concentration dependent way. Conclusion Hp could effect the gastric epithelial cells apoptosis and proliferation directly and influence the expression of p27kips protein which might facilitate gastric carcinoma. 相似文献
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Objective To explore the influence of Helicobacter pylori (Hp) on the proliferation, apoptosis and expression of p27kipl protein of gastric epithelial cells in vitro. Methods SC, C-7901 cell pro-liferation was examined by flow cytometry after incubation with different concentration of NCTC11637. The effect of NCTC11637 on cell apoptosis was also evaluated by flow cytometryo And the expression of p27kipl of gastric epithelial cells was detected by immunohistochemical analysis and in situ hybridization, respectively. Results Cell proliferation was enhanced when co-incubated with the Hp in low concentration (3.4 x 104 to 1.9 x 105 CFU/ml) but inhibited in higher concentration (≥4.8×106CFU/ml). However, cell apoptosis was increase when co-incubated with the Hp in high concentration (≥9.6 ×105 CFU/ml) showing concen-tration dependent picture. In addition, the co-incubation of SGC-7901 with Hp led to decrease of the expres-sion of p27kipl protein but not mRNA in a Hp concentration dependent way. Conclusion Hp could effect the gastric epithelial cells apoptosis and proliferation directly and influence the expression of p27kips protein which might facilitate gastric carcinoma. 相似文献
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Objective To explore the influence of Helicobacter pylori (Hp) on the proliferation, apoptosis and expression of p27kipl protein of gastric epithelial cells in vitro. Methods SC, C-7901 cell pro-liferation was examined by flow cytometry after incubation with different concentration of NCTC11637. The effect of NCTC11637 on cell apoptosis was also evaluated by flow cytometryo And the expression of p27kipl of gastric epithelial cells was detected by immunohistochemical analysis and in situ hybridization, respectively. Results Cell proliferation was enhanced when co-incubated with the Hp in low concentration (3.4 x 104 to 1.9 x 105 CFU/ml) but inhibited in higher concentration (≥4.8×106CFU/ml). However, cell apoptosis was increase when co-incubated with the Hp in high concentration (≥9.6 ×105 CFU/ml) showing concen-tration dependent picture. In addition, the co-incubation of SGC-7901 with Hp led to decrease of the expres-sion of p27kipl protein but not mRNA in a Hp concentration dependent way. Conclusion Hp could effect the gastric epithelial cells apoptosis and proliferation directly and influence the expression of p27kips protein which might facilitate gastric carcinoma. 相似文献
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提高医学生临床实习质量的对策 总被引:4,自引:1,他引:3
临床实习是临床医学生实践能力培养的黄金阶段,目前受多种因素影响,临床实践的质量很难得到有效保证。临床实习病员相对缺乏是导致这一现状的重要因素。就引起病员不足的主要影响因素,如医学生的大规模扩招、现有病员不能充分利用等进行了分析,并提出扩大临床实习基地建设、充分认识临床实习生的劳动价值、大力宣传医学知识及加强医患关系的培养等相关建议,为医学生临床实习质量的提高提供参考。 相似文献