特发性肺纤维化急性加重期证候与血清生物标志物的相关性研究＊

目的 通过分析特发性肺纤维化急性加重期（AE-IPF）患者证候与血清生物标志物的关系，为中医辨证治疗提供参考。方法 采用观察性研究设计，收集2019年3月至2019年11月三个中心的AE-IPF患者76例，其中痰热壅肺证26例、痰浊阻肺证50例，并纳入健康志愿者10例作为对照。采用ELISA测定患者血清CCL18、HMGB1、KL-6、MMP-7、SP-A和SP-D水平，分析与中医证候的相关性。结果 AE-IPF患者血清CCL18、HMGB1、KL-6、MMP-7、SP-A和SP-D水平均显著高于健康对照组。血清CCL18、HMGB1、KL-6、MMP-7和SP-D水平在痰热壅肺证和痰浊阻肺证患者间无显著性差异（P＞0.05），而血清SP-A水平存在显著性差异（P＜0.05）。结论 血清SP-A与AE-IPF证候存在一定的相关性，血清SP-A的浓度升高，与痰热壅肺证关系越密切，反之，血清SP-A浓度降低，则与痰浊阻肺证关系越密切。AE-IPF痰热壅肺证患者的预后可能较痰浊阻肺证患者更差。     

刮痧力度及时间间隔对刮痧效果的影响

目的:观察不同力度对刮痧效果的影响,初步探讨刮痧的作用机制以及效应周期,进一步探究刮痧治疗的最佳间隔时间。方法:用自制的特殊刮痧板控制使刮痧频次等参数保持一致,分别以1N、3N和9N的力进行刮痧,通过ELISA方法检测刮痧后IL-1α、IL-6的变化,结合皮肤HE染色法探究不同力度刮痧对机体的影响,选择适合的刮痧力度,通过血常规和流式细胞术检测刮痧后不同时间点的白细胞总数、淋巴细胞总数及T细胞亚群等的变化,初步探讨刮痧的作用机制和效应周期。结果:1N的力度刮痧未引起明显的变化,9N的力度刮痧造成了较严重的皮肤损伤和大量炎性反应因子释放,3N的力度刮痧较为适中,引起了机体适度的应激反应。以3N的力度刮痧后,血液中白细胞总数和淋巴细胞总数及IL-1α、IL-6缓慢升高,在1 h到24 h之间达到峰值,之后缓慢下降,在72 h左右恢复正常水平。结论:3N的力比较适合于刮痧实验,在刮痧之后1 h到24 h,刮痧对免疫系统的增强作用达到峰值,在72 h左右回归正常值,初步提示可以开始下一次刮痧治疗。     

Tumor microenvironment characterization identifies two lung adenocarcinoma subtypes with specific immune and metabolic state

The tumor microenvironment (TME) is a vital component of tumor tissue. Increasing evidence suggests their significance in predicting outcomes and guiding therapies. However, no studies have reported a systematic analysis of the clinicopathologic significance of TME in lung adenocarcinoma (LUAD). Here, we inferred tumor stromal cells in 1184 LUAD patients using computational algorithms based on bulk tumor expression data, and evaluated the clinicopathologic significance of stromal cells. We found LUAD patients showed heterogeneous abundance in stromal cells. Infiltration of stromal cells was influenced by clinicopathologic features, such as age, gender, smoking, and TNM stage. By clustering stromal cells, we identified 2 clinically and molecularly distinct LUAD subtypes with immune active and immune repressed features. The immune active subtype is characterized by repressed metabolism and repressed proliferation of tumor cells, while the immune repressed subtype is characterized by active metabolism and active proliferation of tumor cells. Differentially expressed gene analysis of the two LUAD subtypes identified an immune activation signature. To diagnose TME subtypes practically, we constructed a TME score using principal component analysis based on the immune activation signature. The TME score predicted TME subtypes effectively in 3 independent datasets with areas under the receiver operating characteristic curves of 0.960, 0.812, and 0.819, respectively. In conclusion, we proposed 2 clinically and molecularly distinct LUAD subtypes based on tumor microenvironment that could be valuable in predicting clinical outcome and guiding immunotherapy.     

新型冠状病毒肺炎疫情期间肝移植病房管理单中心经验

自2019年12月发生的新型冠状病毒肺炎(COVID-19)影响范围广、感染人数多,在对社会生活造成严重影响的同时,也给医院器官移植病房的管理带来了巨大挑战。肝移植受者术后免疫力低下,新型冠状病毒(2019-nCoV)易感性可能较普通人群更高。因此,肝移植病房的管理具有其特殊性。COVID-19疫情期间,青岛大学附属医院肝脏移植科医护人员通过学习国家卫生健康委员会发布的COVID-19防控和诊疗方案以及相关指南,加强医护人员自身防护,制订适应疫情形势的住院患者管理流程,完善患者及其家属COVID-19相关知识宣教,建立并优化肝移植病房管理方案。期望通过特殊时期肝移植病房的有效管理,在予肝移植受者科学治疗的同时,有效避免2019-nCoV感染。     

Development and Validation of a Metabolic-related Prognostic Model for Hepatocellular Carcinoma

Background and AimsGrowing evidence suggests that metabolic-related genes have a significant impact on the occurrence and development of hepatocellular carcinoma (HCC). However, the prognostic value of metabolic-related genes for HCC has not been fully revealed.MethodsmRNA sequencing and clinical data were obtained from The Cancer Genome Atlas and the GTEx Genotype-Tissue Expression comprehensive database. Differentially expressed metabolic-related genes in tumor tissues (n=374) and normal tissues (n=160) were identified by the Wilcoxon test. Time-dependent receiver operating characteristic curve analysis, univariate multivariate Cox regression analysis and Kaplan-Meier survival analysis were used to evaluate the predictive effectiveness and independence of the prognostic model. Two independent cohorts (International Cancer Genome Consortiums and {"type":"entrez-geo","attrs":{"text":"GSE14520","term_id":"14520"}}GSE14520) were applied to verify the prognostic model.ResultsOur study included a total of 793 patients with HCC. We constructed a risk score consisting of five metabolic-genes (BDH1, RRM2, CYP2C9, PLA2G7, and TXNRD1). For the overall survival rate, the low-risk group had a considerably higher rate than the high-risk group. Univariate and multivariate Cox regression analyses indicated that the risk score was an independent predictor for the prognosis of HCC.ConclusionsWe constructed and validated a novel prognostic model, which may provide support for the precise treatment of HCC.     

MicroRNA-137 inhibits cell migration and invasion by targeting bone morphogenetic protein-7 (BMP7) in non-small cell lung cancer cells

MicroRNA-137 (miR-137) was reported to be dysregulated in several human cancers. However, the function and mechanism of miR-137 in non-small cell lung cancer (NSCLC) is still unclear. In the current study, we explored the role of miR-137 in NSCLC progression. Using qRT-PCR, our data showed that miR-137 was significantly down-regulated in NSCLC tissues and cell lines. In vitro functional assay, we found that over-expression of miR-137 suppressed NSCLC cells proliferation, migration and invasion, indicating that miR-137 could act as a tumor suppressor in NSCLC progression. In addition, bone morphogenetic protein-7 (BMP7) was identified as a target of miR-137 in NSCLC cells, Luciferase reporter assay suggested that miR-137 directly targeted 3’-UTR of BMP7, and correlation analysis revealed that BMP7 inversely correlated with miR-137 in NSCLC tissues. Furthermore, Restoration of BMP7 remarkably reversed the tumor suppressive effects of miR-137 on NSCLC cell proliferation, migration, and invasion. Taken together, our findings suggested that miR-137/BMP7 axis could contribute to the progression of NSCLC, suggesting miR-137 as a potential therapeutic target for the treatment of NSCLC.     

全肘关节置换术的临床应用及疗效分析

 目的 探讨全肘关节置换术治疗老年肱骨远端粉碎性骨折和肘部类风湿关节炎的临床疗效。方法 自2006年4月至2012年6月,应用半限制型全肘关节置换术治疗老年肱骨远端粉碎性骨折9例、肘部类风湿关节炎4例。男3例,女10例;50岁1例,大于60岁12例。肱骨远端骨折AO分型C2型3例、C3型6例。其中8例一期行全肘关节置换,1例开放性骨折（Gustilo-AndersonⅠ型）二期行全肘关节置换。4例肘部类风湿关节炎患者平均病程7.8年,Mayo分期Ⅲ期1例、Ⅳ期2例、Ⅴ期1例。全部采用铰链式-半限制型假体,以骨水泥固定。结果 手术时间120~180 min,平均150 min;出血量250~550 ml,平均400 ml;住院时间16~55 d,平均30.2 d。全部病例获得随访,随访时间15~82个月,平均 39.5个月。12例切口一期愈合,1例类风湿关节炎患者发生急性切口感染。切口感染病例病原学检查为阴沟杆菌,经二期手术清理、置管冲洗引流4周,应用敏感抗生素治疗后痊愈。该例术中截骨时发生肱骨外髁骨折,给予克氏针张力带固定,术后4个月骨性愈合。随访期间全部病例均未发生假体松动、脱位等并发症。X线片示假体位置良好,7例骨水泥界面出现透光区。Mayo肘关节功能评分优3例、良8例、可2例,优良率84.6%（11/13）。结论 全肘关节置换治疗肘部类风湿关节炎和老年肱骨远端粉碎性骨折有效,但必须严格掌握手术适应证和遵循手术操作技巧,治疗肘部类风湿关节炎时术中及术后并发症发生率较高。