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目的 探讨β-淀粉样蛋白25~35(Aβ25-35)急性给药和慢性孵育对神经元Ca2+非依赖性的K+电流作用的区别. 方法 急性分离大鼠海马及培养皮层神经元; Aβ25-35急性给药(3min)或慢性孵育(24h);利用全细胞膜片钳技术记录Ca2+非依赖性的K+电流以及Calcein-AM法检测神经元活力. 结果 Aβ25-35急性给药使急性分离的海马神经元Ca2+非依赖性的K+电流幅度明显降低(n=11),而慢性孵育则使培养的皮层神经元该电流幅度明显升高(n=11).前者是Aβ25-35通过对K+通道直接的效应发挥其抑制作用,而后者可能主要是通过Aβ25-35上调通道蛋白,改变通道数量而发挥作用. 结论 不同的给药方式通过不同的机制对海马和皮层神经元的Ca2+非依赖性的K+电流产生不同的作用. 相似文献
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目的探讨塞来昔布对不同程度骨关节炎患者关节液肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1(interleukin-1,IL-1)、前列腺素E_2(prostaglandin E_2,PGE_2)水平及视觉模拟评分(visual analogue scale,VAS)的影响。方法 180例骨关节炎患者,根据病情分为轻度组65例,中度组60例,重度组55例,3组均在常规治疗基础上给予塞来昔布200mg口服,1次/d,共8周。治疗前及治疗2、4、8周时,采用ELISA法检测3组患者关节液TNF-α、IL-1、PGE_2水平,采用VAS评分观察患者疼痛程度改善情况。结果治疗前重度组关节液TNF-α[(56.78±2.43)ng/L]、IL-1[(125.74±4.43)ng/L]、PGE_2[(49.95±3.43)ng/L]水平及VAS评分[(7.21±0.85)分]均高于轻度组[(29.45±3.25)ng/L、(62.78±4.25)ng/L、(25.78±3.15)ng/L、(4.99±0.94)分]和中度组[(42.45±3.78)ng/L、(95.45±4.78)ng/L、(40.56±3.28)ng/L、(6.23±0.78)分](P0.05),中度组高于轻度组(P0.05);治疗2、4、8周后,3组患者关节液TNF-α、IL-1、PGE_2水平和VAS评分均较治疗前降低(P0.05),轻、中度组关节液TNF-α、IL-1、PGE_2水平及VAS评分降低程度大于重度组(P0.05)。结论塞来昔布可有效抑制TNF-α、IL-1、PGE_2的产生,具有抗炎性反应、缓解疼痛的作用,对轻、中度骨关节炎患者效果佳。 相似文献
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目的:观察短时肾动脉阻断应用于经腹膜后腹腔镜巨大肾上腺肿瘤(≥10cm)切除术的疗效,并探讨该方法的安全性和可行性。方法:回顾性分析我院2015年7月~2018年7月采用短时肾动脉阻断行经腹膜后腹腔镜巨大肾上腺肿瘤切除术的9例巨大肾上腺肿瘤患者的临床资料,观察分析其肾动脉阻断时间、手术时间、估计失血量、肾周引流时间、术后住院时间和术后引流量,并对患者进行随访,观察肿瘤复发及肾功能变化情况。结果:9例患者均顺利完成手术,无中转开放,患者肾动脉阻断时间、手术时间、估计失血量、肾周引流时间、术后住院时间和术后引流量分别为(6.22±2.04)min、(152.6±52.8)min、(127.0±117.55)ml、(4.33±1.41)d、(6.44±1.58)d、(71.66±43.95)ml,无术中并发症,术后病理检查显示嗜铬细胞瘤3例,肾上腺髓质脂肪瘤6例。随访1~35个月未观察到肿瘤复发或转移迹象,患者肾功能无异常。结论:短时肾动脉阻断行经腹膜后腹腔镜巨大肾上腺肿瘤(≥10cm)切除术是可行、有效、安全的方法。 相似文献
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BACKGROUND: Humanin is a 24-amino acid peptide isolated from the brain of an Alzheimer’s disease patient. Several studies have indicated that Humanin can protect cells against cytotoxicity induced by various insults.
OBJECTIVE: To investigate the protective role of Humanin on hypoxia-induced neuronal death, and to determine the most appropriate therapeutic concentration of Humanin.
DESIGN, TIME AND SETTING: Neuropathophysiological, randomized, controlled experiment, conducted at the Department of Physiology and Neurobiology, Shanxi Medical University, between March 2007 and October 2007.
MATERIALS: Newborn Wistar rats, 5,5',6,6' tetrachloro-1,1',3,3'-tetraethyl- benzimidazolylcarbo- cyanine iodide (JC-1, USA), calcein-acetoxymethylester (calcein-AM, USA), and Humanin (Shanghai, China) were used in this study. METHODS: Primary cortical neurons were cultured with dulbecco's modified eagle's medium containing 15% fetal bovine serum. Cultures were divided into three groups: control, hypoxia, and hypoxia + Humanin. Various concentrations of Humanin (1, 10, and 20 μmol/L) were added to the cultures 16 hours prior to hypoxia induction. For hypoxic conditions, cells were maintained at 37 ℃ within an incubator chamber filled with 95% N2 and 5% CO2 for 24 hours. Cells in the control group were cultured in normal oxygen.
MAIN OUTCOME MEASURES: Cell viability was determined through the use of the vital dye calcein-AM, and the number of live cells was determined. Mitochondrial membrane potential (△Ψm) was assessed using the fluorescent probe JC-1. Mitochondrial permeability transition pore (mPTP) opening was determined with calcein-AM in the presence of cobalt chloride.
RESULTS: (1) Cell viability: Hypoxia for 24 hours induced death in a large number of neurons. Pre- treatment with 10 μmol/L and 20 μmol/L Humanin, 16 hours prior to hypoxia, protected cells against hypoxia. However, 1 μmol/L Humanin provided little protection. (2) △Ψm: △Ψm was re-duced after 24-hour hypoxia, as assessed by JC-1 and a confocal microscope. Pretreatment with 20 μmol/L Humanin preserved the loss of △Ψm. (3) mPTP: Hypoxia induced the opening of mPTP. Pretreatment with 20 μmol/L Humanin repressed the opening of mPTP, as most of the calcein fluorescence remained in the mitochondria.
CONCLUSION: Humanin (20 μmol/L) protects neuronal cells from hypoxia-induced insults by in- hibiting the opening of mPTP and preserving △Ψm. 相似文献
OBJECTIVE: To investigate the protective role of Humanin on hypoxia-induced neuronal death, and to determine the most appropriate therapeutic concentration of Humanin.
DESIGN, TIME AND SETTING: Neuropathophysiological, randomized, controlled experiment, conducted at the Department of Physiology and Neurobiology, Shanxi Medical University, between March 2007 and October 2007.
MATERIALS: Newborn Wistar rats, 5,5',6,6' tetrachloro-1,1',3,3'-tetraethyl- benzimidazolylcarbo- cyanine iodide (JC-1, USA), calcein-acetoxymethylester (calcein-AM, USA), and Humanin (Shanghai, China) were used in this study. METHODS: Primary cortical neurons were cultured with dulbecco's modified eagle's medium containing 15% fetal bovine serum. Cultures were divided into three groups: control, hypoxia, and hypoxia + Humanin. Various concentrations of Humanin (1, 10, and 20 μmol/L) were added to the cultures 16 hours prior to hypoxia induction. For hypoxic conditions, cells were maintained at 37 ℃ within an incubator chamber filled with 95% N2 and 5% CO2 for 24 hours. Cells in the control group were cultured in normal oxygen.
MAIN OUTCOME MEASURES: Cell viability was determined through the use of the vital dye calcein-AM, and the number of live cells was determined. Mitochondrial membrane potential (△Ψm) was assessed using the fluorescent probe JC-1. Mitochondrial permeability transition pore (mPTP) opening was determined with calcein-AM in the presence of cobalt chloride.
RESULTS: (1) Cell viability: Hypoxia for 24 hours induced death in a large number of neurons. Pre- treatment with 10 μmol/L and 20 μmol/L Humanin, 16 hours prior to hypoxia, protected cells against hypoxia. However, 1 μmol/L Humanin provided little protection. (2) △Ψm: △Ψm was re-duced after 24-hour hypoxia, as assessed by JC-1 and a confocal microscope. Pretreatment with 20 μmol/L Humanin preserved the loss of △Ψm. (3) mPTP: Hypoxia induced the opening of mPTP. Pretreatment with 20 μmol/L Humanin repressed the opening of mPTP, as most of the calcein fluorescence remained in the mitochondria.
CONCLUSION: Humanin (20 μmol/L) protects neuronal cells from hypoxia-induced insults by in- hibiting the opening of mPTP and preserving △Ψm. 相似文献
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基于生理学课程特点,构建包括病例模式、核心问题模式、情景故事模式等的多方位PBL教学模式,涉及到教学载体构建、教材资料编写、课堂教学程序和效果评价体系等环节。 相似文献
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杨小荣 《实用中西医结合临床》2022,22(21)
目的 观察化斑汤治疗上皮性卵巢癌(EOC)多腺苷二磷酸核糖聚合酶(PARP)抑制剂引起血小板减少的临床价值。方法 依据随机数字表法将2021年7月-2022年7月江西省肿瘤医院接收的80例EOC经PARP抑制剂治疗引起血小板减少的患者分为对照组、治疗组,各40例,对照组进行西医对症治疗,治疗组进行口服化斑汤联合西医治疗,治疗3个疗程后,比较两组临床疗效、血小板计数(PLT)恢复至正常时间;于治疗前、后,比较两组临床症状评分、中医证候积分、免疫功能指标(CD3+、CD4+、CD8+T淋巴细胞水平)。结果 治疗组总有效率为97.50%,高于对照组的80.00%,有统计学差异(P<0.05);治疗后,两组牙龈出血、鼻衄等临床症状各评分显著下降,且治疗组低于对照组,有统计学差异(P<0.05);治疗后,两组中医证候积分显著降低,治疗组低于对照组,且治疗组PLT恢复至正常时间短于对照组,均有统计学差异(P<0.05);治疗后,两组CD3+、CD4+水平显著升高,CD8+显著下降,且治疗组CD3+、CD4+高于对照组,CD8+低于对照组,有统计学差异(P<0.05)。结论 化斑汤治疗EOC经PARP抑制剂治疗引起血小板减少的患者,可提高疗效,减轻患者临床症状,促进PLT恢复,提高患者免疫功能。 相似文献
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目的探究三氧对抑郁症模型小鼠行为学以及脑内海马、额叶和杏仁核小胶质细胞数量与炎症因子水平的影响。方法将40只小鼠随机分为4组,模型组、模型加三氧组、对照组和对照加三氧组,每组10只。对模型组和模型加三氧组小鼠进行连续7 d,每天1 h的束缚应激制备抑郁症小鼠模型,其中模型加三氧组和对照加三氧组采用三氧水(80 μg/mL)腹腔注射。造模前3天给予三氧作为三氧预防性干预,造模成功后给予三氧作为三氧治疗性干预。利用悬尾实验、强迫游泳实验、新环境抑制进食实验和开放旷场实验检测各组小鼠行为学变化。小胶质细胞标记物IBA1免疫组化染色检测海马、额叶和杏仁核小胶质细胞数量的改变。逆转录聚合酶链式反应技术检测海马、额叶和杏仁核炎症因子白介素(interleukin,IL)-1β、IL-6和肿瘤坏死因子(tumer necrosis factor-α,TNF-α)的表达水平。结果三氧预防性干预中:三氧能改善小鼠抑郁行为,减少脑内额叶、海马区激活的小胶质细胞数量,降低炎症因子IL-1β、TNF-α的水平。三氧治疗性干预中:三氧未能改善小鼠抑郁行为,模型加三氧组与模型组额叶、海马、杏仁核区激活的小胶质细胞数量比较差异无统计学意义(P>0.05),三氧治疗性干预未能降低额叶、海马区炎症因子IL-1β、TNF-α的水平。结论三氧腹腔注射对预防小鼠抑郁症形成有一定的作用,但无治疗作用。 相似文献