Analysis of the correlation between P27 expression and Helicobacter pylori infection in gastric cancer

We aimed to explore the correlation between P27 expression and Helicobacter pylori (H. pylori) infection in gastric cancer, so as to provide evidence for understanding the pathogenesis of gastric cancer caused by H. pylori infection. A total of 82 samples of gastric cancer tissues and 56 samples of tumor-adjacent normal tissues collected from the gastrectomy were enrolled in this study. Then, 14C-urease breathing test was carried out to evaluate the infection of H. pylori in gastric cancer tissues, the expression of P27 in the tissue samples was detected by the immunohistochemistry staining, and the correlation between the H. pylori infection and P27 expression in gastric cancer was analyzed. Of 82 gastric cancer patients, there were 53 patients with H. pylori infection (64.63%). Among the patients with highly or moderately differentiated gastric cancer, the expression of P27 was much higher than that of patients with poorly differentiated gastric cancer (p < 0.01). Besides, comparison of the P27 expression between males and females, among different age groups, tumor sizes, TNM stages, tumor infiltration degrees, or lymph node metastasis, showed no significant differences (p > 0.05). Analysis of the correlation revealed that P27 expression was negatively correlated with the infection of H. pylori (p < 0.01). Multifactorial logistics regression analysis indicated that tumor differentiation was a risk factor of P27-positive expression in gastric cancer tissues (p < 0.01). In addition, P27 expression in the gastric cancer tissues was lower than that in the tumor-adjacent normal tissues (p < 0.01). In gastric cancer patients, expression of P27 is correlated with H. pylori infection which, via downregulating P27, can cause the cancerization of gastric mucosa, and P27, for its role in the development and progression of gastric cancer, is a potential auxiliary indicator for clinical diagnosis whether gastric cancer is complicated with H. pylori infection. So, P27 is a key indicator for diagnosis, treatment, and prognostic evaluation of disease in the advanced stage.     

2014年山西省肿瘤登记地区恶性肿瘤发病与死亡分析

目的探讨2014年山西省肿瘤登记地区恶性肿瘤发病与死亡情况。方法收集2014年全省12个肿瘤登记地区的数据资料,分析不同地区、不同年龄和性别的恶性肿瘤发病率与死亡率特点,同时与全国数据进行比较。结果2014年山西省肿瘤登记地区新发恶性肿瘤11703例(男性6559例,女性5144例),发病率为221.21/10万,中国人口标化发病率为163.91/10万,世界人口标化发病率为163.25/10万;城市地区发病率、中国人口标化发病率分别为247.02/10万、171.35/10万,农村地区分别为205.98/10万、159.03/10万。男性常见恶性肿瘤发病以胃癌、肺癌、食管癌、肝癌、结直肠癌为主,女性以乳腺癌、子宫颈癌、肺癌、食管癌、胃癌为主。恶性肿瘤死亡病例7283例(男性4548例,女性2735例),恶性肿瘤死亡率为137.66/10万,中国人口标化死亡率为99.67/10万,世界人口标化死亡率为100.11/10万;城市地区死亡率、中国人口标化死亡率分别为141.03/10万、92.84/10万,农村地区分别为135.68/10万、103.69/10万。男性常见恶性肿瘤死亡以肺癌、胃癌、肝癌、食管癌、结直肠癌为主,女性以肺癌、胃癌、肝癌、食管癌、子宫颈癌为主。结论山西省肿瘤登记地区恶性肿瘤发病与死亡均以肺癌、上消化道恶性肿瘤和女性子宫颈癌为主,胃癌、子宫颈癌的发病率较高。     

益母草制剂的开发与应用

益母草制剂在临床中应用广泛,主要用于多种妇产科疾病的治疗,且效果显著。其中,益母草注射剂、益母草颗粒、益母草片是临床上最常见的益母草剂型,其有效性和安全性在长期的临床实践中得到认可。本论文结合项目组对益母草制剂的二次开发研究思路,对上述具有代表性的益母草制剂的制剂工艺、质量控制和临床应用进行介绍,以期为益母草制剂的现代研究和临床安全应用提供一定的参考。     

Comparison of microtransplantation,chemotherapy and allogeneic transplantation in post-remission therapy for Philadelphia chromosome-positive acute lymphoblastic leukemia

Patients with Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph⁺ ALL) have poor prognosis, and the efficacy of chemotherapy plus tyrosine kinase inhibitors (TKIs) followed by mismatched donor stem cell infusion (microtransplantation, MST) has not been determined. We retrospectively summarized 45 patients including 11 undergoing MST with TKIs, 17 receiving allogeneic transplant and 17 undergoing chemotherapy with TKIs. Improved 4-year overall survival rate was observed in the MST group (91%) compared with either transplant group (31%, P = .005) or chemotherapy group (36%, P = .013). The MST group also had higher 2-year and 4-year leukemia-free survival rates (91% and 72%, respectively) compared with either transplant group (33%, P = .005 and 33%, P = .021, respectively) or chemotherapy group (41%, P = .017 and 31%, P = .023, respectively). 2-year and 4-year cumulative incidences of hematologic relapse were lower in the MST group (9% and 28%, respectively) compared with those in the chemotherapy group (56%, P = .025 and 67%, P = .034, respectively). In patients undergoing MST, donor microchimerism was detected (1.07 × 10^-5 to 6.6 × 10^-4 copies from 9 to 1499 days) in 7 patients, and donor/patient-derived HLA*0201/2402⁺WT1⁺CD8⁺ T cells were found from 0.05% to 0.67% in 6 patients. MST may provide a favorable treatment for patients with Ph⁺ ALL.     

重组戊型肝炎病毒P179-海藻酸钠/壳聚糖微球混悬制剂的制备及其免疫效果

目的 制备吸附重组戊型肝炎病毒(hepatitis E virus,HEV)P179抗原的海藻酸钠/壳聚糖微球混悬制剂,并观察其免疫效果。方法 乳化法制备海藻酸钠/壳聚糖微球,采用不同海藻酸钠浓度、混合乳化剂添加量、混合乳化剂亲水亲油平衡值设计正交试验,确定最佳制备工艺参数。将重组HEV P179抗原吸附在最佳工艺制备的微球表面,制备吸附重组HEV P179的海藻酸钠/壳聚糖微球混悬制剂,皮下多点免疫BALB/c小鼠,测定其诱导小鼠产生特异性IgG抗体的能力,与同剂量含弗氏佐剂的重组HEV P179免疫制剂对比。结果 经正交试验确定乳化最佳工艺参数为:海藻酸钠质量分数1%、混合乳化剂体积分数2%,混合乳化剂亲水亲油平衡值4。制备的微球平均粒径为6.73 μm(分布范围3.90~9.10 μm,方差1.78 μm),形态较为均一,透射电镜观察结果与双层球体及内部疏松多孔的微球结构特点相符。用此条件制备抗原质量浓度为500 μg/ml的混悬制剂,微球对抗原的吸附率为90.64%。免疫效果观察试验结果表明,皮下多点免疫试验中微球制剂诱导特异性IgG抗体的能力优于含弗氏佐剂抗原。结论 成功制备了吸附重组HEV P179的海藻酸钠/壳聚糖微球混悬制剂,且诱导产生特异性IgG抗体的能力优于含弗氏佐剂抗原,为其在新型实验动物超敏制剂中的应用奠定了基础。     

医疗领域人工智能应用的研究进展＊

人工智能引发了医疗领域的数字革命，在推动行业发展方面具有极大潜力。本研究围绕临床诊疗、医学研究和公共卫生三个基本场景，聚焦人工智能与传统中医药的交叉与融合，并着重介绍人工智能在疾病诊断、决策支持、医学研究以及重大公共卫生事件中的应用。虽然人工智能在诸多方面显示出独特优势，但仍存在透明度不高、缺乏安全性评估和相关法律法规监管等问题需要谨慎解决，以促进人工智能技术在医疗领域的推广。     

彝药益元化腐汤浸膏对溃疡性结肠炎模型T细胞亚群干预机制研究*

目的研究彝药益元化腐汤调控T细胞亚群介导治疗溃疡性结肠炎的效应机制，为益元化腐汤临床应用治疗溃疡性结肠炎提供科学基础。方法 32只C57BL/6健康雌性小鼠随机分为正常组、模型组、益元化腐汤中剂量（4.05 g·kg-1）和高剂量组（8.1 g·kg-1），每组8只。除正常组，其余以自由饮水的方式给予3%葡聚糖硫酸钠（sodium dextran sulfate，DSS）诱导建立溃疡性结肠炎小鼠模型，每2 d更换1次饮用水，自饮用DSS第1天起，各组按相应剂量灌胃给药，每日1次，连续8 d；采用疾病活动指数（diseases activity index，DAI）和苏木精-伊红染色法（hematoxylin-eosin staining，HE）分别评价溃疡性结肠炎症状及结肠组织形态学变化；荧光定量PCR（qPCR）检测淋巴细胞T细胞亚群相关因子转录的相对表达水平；流式细胞术（flow cytometry）检测T细胞亚群中辅助性T细胞1（T-helper 1 cell, Th1）、辅助性T细胞17（T-helper 17 cell，Th17）和调节性T细胞（regulatory T cell，Treg）的表达水平。结果 与正常组比较，病理模型组小鼠体质量和结肠质量显著下降、结肠长度显著缩短（P<0.05或P<0.01）；与病理模型组比较，益元化腐汤组显著增加小鼠体质量、结肠质量及其长度（P<0.05或P<0.01），改善结肠病理损伤程度；流式细胞术检测结果显示，益元化腐汤药物干预显著下调γδTCR表达（P<0.05），且抑制Th1细胞（CD4+ IFN-γ+ T细胞）、Th17细胞（CD4+ IL-17+ T细胞）绝对数量和比例（P<0.01），然而对Treg细胞（CD4+ Foxp3+ 调节性T细胞）表达无明显影响。qPCR检测结果显示，益元化腐汤显著下调UC小鼠Th17细胞因子IL-17A和转录因子RORγτ mRNA表达（P<0.01）。结论 彝药益元化腐汤可能通过抑制Th17细胞诱导的炎症反应介导治疗溃疡性结肠炎。     

Clinical Implications of HSC70 Expression in Clear Cell Renal Cell Carcinoma

Purpose: The role of heat shock protein 70 (HSC70) in the progression of clear cell renal cell carcinoma (ccRCC) is unclear. This study explored the effect of the HSC70 on the survival of ccRCC patients.Methods: Immunohistochemical analysis was performed to determine HSC70 expression in samples obtained from 121 ccRCC patients with at least 5 years of follow-up. We also analyzed the association between HSC70 expression and clinicopathological characteristics. Furthermore, the association of overall survival (OS) with HSC70 expression was analyzed using Kaplan-Meier curves. Finally, we used the Oncomine and CCLE databases to determine the effects of HSC70 mRNA expression on ccRCC.Results: HSC70 expression was associated with distant metastasis and death of ccRCC patients. HSC70 was expressed in the nucleus and/or cytoplasm of ccRCC cells. The incidence of distant organ metastasis and death was higher in patients with HSC70 expression than in those without it. Survival analysis revealed that patients with HSC70 expression had significantly shorter OS. Oncomine analyses also showed that the HSC70 mRNA was significantly upregulated in ccRCC tissues.Conclusions: HSC70 expression was related to adverse prognosis, and patients with HSC70 expression had a worse prognosis than those without HSC70 expression. HSC70 may thus serve as a potential therapeutic target for ccRCC.     

创伤性脊柱骨折合并脊髓损伤患者血清pNF-H动态变化及其与脊髓损伤程度的相关性

目的 探讨创伤性脊柱骨折合并脊髓损伤患者血清磷酸化高分子量神经微丝蛋白(pNF-H)动态变化与脊髓损伤程度的相关性.方法 回顾性分析2017年1月—2019年1月平煤神马医疗集团总医院脊柱外科收治的创伤性脊柱骨折患者94例,其中合并脊髓损伤48例(脊髓损伤组),男性35例,女性13例;年龄18～60岁,平均50.7岁;致伤原因:道路交通伤20例,摔伤16例,高处坠落伤7例,砸伤5例.单纯脊柱骨折46例(单纯骨折组),男性30例,女性16例;年龄18～60岁,平均50.8岁;致伤原因:道路交通伤18例,摔伤13例,高处坠落伤8例,砸伤7例.选取同期笔者医院进行体检的健康者40例作为健康对照组,男性30例,女性10例;年龄18～60岁,平均50.8岁.比较各组间伤后12h、24h、3d、7d、14d血清pNF-H浓度变化;并分析脊髓损伤组不同美国脊髓损伤协会(ASIA)神经功能分级患者12h、24h、3d、7d、14d时血清pNF-H浓度变化,采用Spearman检验分析血清pNF-H动态变化与ASIA神经功能分级的相关性.结果 伤后12h、24h、3d、7d、14d,脊髓损伤组血清pNF-H浓度高于相应时间点单纯骨折组、健康对照组(P<0.05),单纯骨折组血清pNF-H浓度高于相应时间点健康对照组(P<0.05).不同ASIA分级患者不同时间点血清pNF-H浓度比较差异有统计学意义(P<0.05);在12h、24h、3d、7d、14d时A级患者血清pNF-H浓度高于B、C、D级(P<0.05),B级患者血清pNF-H浓度高于C、D级(P<0.05),C级患者血清pNF-H浓度高于D级(P<0.05);血清pNF-H与A-SIA分级存在负相关(r=-0.951,P<0.05).结论 创伤性脊柱骨折合并脊髓损伤患者血清pNF-H浓度随时间变化,而ASIA分级越严重血清pNF-H浓度越高.