近似人病理生理过程动物脂肪肝模型的综合建立方法

Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet.     

近似人病理生理过程动物脂肪肝模型的综合建立方法

Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet.     

近似人病理生理过程动物脂肪肝模型的综合建立方法

Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet.     

近似人病理生理过程动物脂肪肝模型的综合建立方法

目的建立近似人病理生理过程的动物脂肪肝模型,使之适用于影像学定量诊断或药物疗效的验证研究。方法健康新西兰大白兔45只,随机分为四组：正常对照组10只,普通饲料及水喂养9周;实验1、2、3组,各10只,采用高脂高糖饲料＋酒精饮料分别喂养3周、6周和12周;补充组5只,给予实验组相同的喂养和处理。采用HE染色和苏丹Ⅳ染色对动物肝脏进行组织学检查。结果实验成模率为87％（39／45）,死亡率为13％（6／45）,出现正常肝10只,轻度脂肪肝10只,中度脂肪肝9只,重度脂肪肝10只（HE染色）,与苏丹Ⅳ染色Vv值分级的诊断符合率约为75％。结论综合运用高脂高糖饲料＋酒精饮料可成功建立近似人病理生理过程的动物脂肪肝模型。     

血生化、超声、CT、1H MRS定量诊断脂肪肝的综合对照实验研究

目的比较生化、超声、CT、1H MRS检查在脂肪肝诊断中的临床价值。方法选用45只健康新西兰大白兔,采用高脂高糖饲料加酒精饮料建立不同程度脂肪肝模型,应用生化、超声、CT及1H MRS多种临床诊断手段,观测多个变量指标,并进行对照研究。组间差异采用单因素方差分析和LSD检验,相关性采用等级相关分析。结果在所选择的18个观测指标中,血清谷草转氨酶、胆固醇、甘油三脂、超声衰减系数、肝CT值、肝/竖脊肌密度比率、1H MRS脂肪峰值、脂肪波峰下面积、脂/水波峰下面积比率、脂肪百分比等10个指标存在组间差异性,且与脂肪肝分级具有显著性相关,其中血清胆固醇、超声衰减系数、肝CT值和1H MRS脂肪曲线下面积分别是生化、超声、CT、MRS诊断方法中与脂肪肝分级相关系数最高的观测指标,相关系数分别为0.886、0.483、-0.764、0.558。结论在脂肪肝的临床诊断中,生化、CT检查的应用价值高于超声波和1HMRS检查。     

兔脂肪肝的综合定量诊断

目的 寻找和筛选脂肪肝定量诊断的最佳预测指标,建立多指标联合定量诊断脂肪肝的新方法.方法 选用45只健康新西兰大白兔,采用高脂高糖饲料加酒精饮料建立不同程度脂肪肝模型,应用大体观测、血生化、超声(US)、计算机断层扫描(CT)及质子磁共振波谱(1H-MRS)多种临床诊断手段,对22个观测指标进行筛选,寻找脂肪肝定量诊断的最佳预测指标,并建立脂肪肝综合定量诊断的数学模型;通过ROC曲线分析比较多指标联合应用与单指标应用诊断脂肪肝的价值.结果 肝脏湿重、肝脏指数、肝CT值、肝/腰大肌密度比率、1H-MRS脂肪峰值、脂肪波峰下面积、脂/水波峰下面积比率、脂肪百分比、超声衰减系数、谷草转氨酶(AST)、胆固醇(TC)、甘油三脂(TG)等12个指标存在组间差异性,且与脂肪肝分级诊断具有显著性相关,其中肝脏指数、肝CT值和血TC为脂肪肝分级诊断的最佳预测指标,数学模型为Y=1.98+3.91×10-2X1+0.37X2-2.84×10-2X3(Y:脂肪肝分级;X1:血TC;X2:肝脏指数;X3:肝CT值);ROC分析显示多指标联合应用(Y)诊断脂肪肝的价值优于单指标应用.结论 联合应用多种诊断手段,综合考虑多个最佳预测指标的综合诊断方法,能够为临床快速、准确地判断脂肪肝程度提供帮助.     

影像学技术定量诊断脂肪肝的实验研究

目的 利用ROC曲线比较生化、超声、CT、质子磁共振波谱(1H MRS)定量诊断脂肪肝的应用价值.方法 选用45只健康新西兰大白兔,高脂高糖饲料加酒精饮料建立不同程度脂肪肝模型,应用生化、超声、CT及1H MRS多种.临床诊断方法,共观测18个变量指标,在每种诊断方法所观测的指标中,选取与脂肪肝分级相关性最高的指标,并以此进行受试者操作特征(ROC)曲线分析和ROC曲线下面积Z检验,比较这几种诊断方法的应用价值.结果 在所有18个观测指标中,血清胆固醇、超声衰减系数、肝CT值和1H MRS脂肪波峰下面积分别是生化、超声、CT、1H MRS诊断方法中与脂肪肝分级相关性最高的观测指标,相关系数分别为0.886(P=0.000)、0.483(P=0.002)、-0.764(P=0.000)、0.558(P=0.001);ROC曲线分析和Z检验显示:上述各指标ROC曲线下面积分别为0.981,0.581,0.810,0.713,除1H MRS脂肪波峰下面积与超声衰减系数、肝CT值之间差异无统计学意义外(P>O.05),其他两两之间差异均有统计学意义(P1H MRS>超声;生化测定血清胆固醇水平对判断脂肪肝程度可能具有重要的意义,值得进一步研究.     

STEAM和PRESS序列在脂肪肝定量诊断中应用价值的对比研究

目的比较STEAM和PRESS序列在脂肪肝定量诊断中的应用价值。方法选用45只健康新西兰大白兔,采用高脂高糖饲料加酒精饮料建立不同程度脂肪肝模型,采用STEAM和PRESS两种脉冲序列对同一位置、同一体素进行磁共振氢波谱扫描,共观察和测定9个指标,并逐一进行配对对照研究。结果经STEAM和PRESS两种定位方法测定,肝内水化学位移、脂肪化学位移、脂/水峰值比率、脂/水波峰下面积比率、脂肪百分比组间均数无显著性差异（P〉0.05）;而肝内水峰峰值、脂峰峰值、水波峰下面积、脂波峰下面积组间均数存在显著性差异（P〈0.05）,即STEAM序列下上述各指标均数明显低于PRESS序列,信号相对损失率平均约47.9%;且STEAM序列下各观测指标与脂肪肝病理分级的相关性普遍低于PRESS序列。结论PRESS较STEAM序列更适用于肝内脂肪含量的定量研究。     

筛选脂肪肝分级诊断最佳预测指标的实验研究

目的筛选和寻找脂肪肝分级诊断的最佳预测指标。方法45只健康新西兰大白兔，采用高脂高糖饲料加酒精饮料建立不同程度脂肪肝模型，应用肉眼观测、血生化、超声、计算机断层扫描（CT）及氢磁共振波谱（1H MRS）多种临床诊断手段，观测22个指标并进行对照研究。结果在所选择的22个观测指标中，肝脏湿重、肝脏指数、肝CT值、肝／腰大肌密度比率、1HMRS脂肪峰值、脂肪波峰下面积、脂／水波峰下面积比率、脂肪百分比、超声衰减系数、谷草转氨酶（AST）、血清胆固醇（CHOL）、三酰甘油（TG）等12个指标总体均数存在组间差异性，且与脂肪肝分级具有显著性相关，其中肝脏指数、肝CT值和血清胆固醇为脂肪肝分级诊断的最佳预测指标，相关系数分别为0．709，-0．764，0．886，判断脂肪肝分级的回归方程Y=1．975＋3．906×10^-2X1＋0．369X2-2．84×10^-2X3，Y：脂肪肝分级，X1：血清胆固醇，X2：肝脏指数，X3：肝CT值。结论联合应用多种诊断手段，综合考虑多个最佳预测指标的诊断方法，能够为临床快速、准确地判断脂肪肝程度提供帮助。