中等强度旋转恒磁场亚慢性连续照射对大鼠大脑氧化还原和组织病理的影响

目的:探讨中等强度旋转恒磁场亚慢性连续照射对大鼠大脑组织氧化还原指标和组织病理的影响。方法:将22只健康成年雄性Wistar大鼠(购于南方医科大学实验动物中心),体重(220—250)g,随机分成4组:空白对照组(n=5):正常饲养,不给予任何干预;不旋转组(n=5):分别将每只大鼠放置在一个长宽高分别为10cm、10cm、12cm的均匀分布着孔洞的塑料筐里,可以保证在实验时大鼠一直在磁场上方,塑料筐上盖有玻璃板,防止大鼠跳出,然后将大鼠放于磁场上方(0.49T),磁场不旋转,实验期间整只大鼠都暴露于磁场当中;低频旋转组(n=6):将大鼠放于旋转恒磁场上方(0.49T),旋转频率为3000rpm;高频旋转组(n=6):将大鼠放于旋转恒磁场上方(0.49T),旋转频率为6000rpm,每天3h,连续30d。实验结束后取大脑组织进行生化指标丙二醛(MDA)、谷胱甘肽(GSH)、一氧化氮(NO)、髓过氧化物酶(MPO)检测和大脑组织HE染色。结果:各组大鼠大脑组织的MDA、GSH、NO、MPO指标差异无显著性意义(P0.05),组织病理切片未见明显病变。结论:中等强度旋转恒磁场亚慢性连续照射对大鼠大脑组织无明显影响。     

椎体骨水泥注入联合抗骨质疏松药物治疗急性骨质疏松性椎体压缩性骨折

背景：经皮椎体后凸成形治疗椎体压缩性骨折疗效显著,具有创伤小、出血与并发症少、术后恢复快、大部分老年患者可以耐受等特点,但其只是一种治疗手段,并不能有效预防老年患者术后再次发生椎体压缩性骨折。目的：评估经皮椎体后凸成形联合抗骨质疏松药物治疗急性骨质疏松性椎体压缩性骨折的临床疗效。方法：纳入急性骨质疏松性椎体压缩性骨折患者137例（198个治疗椎体）,其中女111例,男26例,年龄（75.55±6.96）岁,行椎体内注射聚甲基丙烯酸甲酯骨水泥经皮椎体后凸成形治疗,治疗后连续3个月服用抗骨质疏松药物。治疗前、治疗后1周、治疗后3个月检查目测类比评分、骨折椎体高度恢复率、Oswestry功能障碍指数、Cobb角,同时记录并发症发生率及椎体压缩性骨折再发生率。结果与结论：所有患者治疗后1周、治疗后3个月的目测类比评分、骨折椎体高度恢复率、Oswestry功能障碍指数、Cobb角均较治疗前明显改善（P 〈0.001）。术后并发症发生率为0.7%,3个月随访期间未再发生椎体压缩性骨折。表明经皮椎体后凸成形联合抗骨质疏松药物可有效治疗急性骨质疏松性椎体压缩性骨折。     

TAp73和Beclin1在人骨肉瘤中表达及其临床意义

目的检测抑癌基因TAp73与自噬相关因子Beclin1在人骨肉瘤中的表达,探讨TAp73与肿瘤自噬的关系。方法采用免疫组织化学SP法检测TAp73、Beclin1在48例骨肉瘤患者的瘤体组织中的表达,结合患者的临床资料进行相关性分析。结果 TAp73在人骨肉瘤组织中的阳性率54.17%,Beclin1的阳性率为62.50%,TAp73阳性表达,其Beclin1表达程度较高(Z=-2.146,P=0.032)。在人骨肉瘤中TAp73的表达程度与Beclin1的表达程度呈正相关(r=0.470,P=0.001)。TAp73、Beclin1阳性表达的患者总体生存率优于阴性表达(80.8%vs 50.0%,P=0.000;80.0%vs 44.4%,P=0.001)。TAp73、Beclin1不同表达程度与患者的生存时间呈正相关(r=0.696,P=0.00;r=0.372,P=0.009)。结论在人骨肉瘤中TAp73可能与骨肉瘤中Beclin1依赖的肿瘤自噬活性上调有关。检测TAp73和Beclin1在人骨肉瘤中的表达水平有助于患者临床预后判断。     

经皮椎体成形修复非骨质疏松单节段椎体创伤性压缩骨折

BACKGROUND: It is still controversial about whether percutaneous vertebroplasty can be as an option for treatment of non-osteoporotic single-segmental vertebral traumatic compression fractures.OBJECTIVE: To observe the effect of percutaneous vertebroplasty in repair of non-osteoporotic single-segmental vertebral traumatic compression fractures.METHODS: Totally 20 patients who underwent percutaneous vertebroplasty in repair of non-osteoporotic single-segmental vertebral traumatic compression fractures between March 2010 and January 2013 were collected. The variation of visual analog scale scores and the Oswestry disability index scores of patients was observed before and after the repair.RESULTS AND CONCLUSION:(1) The visual analog scale scores and the Oswestry disability index scores of patients were significantly reduced after repair compared with those before repair, moreover, the visual analog scale scores and the Oswestry disability index scores of patients at the 3, 6, 12 and 18 months after repair were similar. (2) All patients had no adverse effects and complications. (3) These results suggest that percutaneous vertebroplasty in repair of non-osteoporotic single-segmental vertebral traumaticcompression fractures quickly