Effects of α1-adrenergic receptor on the proliferation of cholangiocarcinoma cells

Objective To investigate the correlation between α1-adrenergic receptor and the pathological behavior of cholangiocarcinoma, and the effects of norepinephrine (NE) on the proliferation of cholangiocarcinoma cell line QBC939. Methods Thirty-six samples of cholangiocarcinoma were resected in Southwest Hospital from August 2002 to March 2008. The expression of α1-adrenergic receptor in the 36 samples of cholangiocarcinoma tissue and 4 samples of normal bile duct tissue were detected by SABC technique. The proliferation of cholangio-carcinoma cell line QBC939 was detected after processing the cells with NE, phentolamine and prazosin. All the data were analyzed by chi-square test. Results The high positive expression rate of α1-adrenergic receptor was 68% (17/25) in patients with lymph node metastasis, which was significantly higher than 9% (1/11) in patients without lymph node metastasis (χ2=10.604, P<0.05). The high positive expression rate of α1-adrenergic receptor was 85% (11/13) in patients with middle and low positioned cholangiocarcinoma, which was significantly higher than 30% (7/23) in patients with hilar cholangiocarcinoma (χ2=9.753, P<0.05). NE promoted the proliferation of cholangiocarcinoma cell line QBC939 by stimulating the expression of α1-adrenergic receptor, and in a concentration-dependent manner. The proliferative effect was weakened as time passed by, and it was eliminated by phentolamine and prazosin. Conclusions The expression of α1-adrenergic receptor is diverse due to lymph node metastasis and the location of the tumor, α1-adrenergic receptor with high expression may play an important role in the proliferation and metastasis of cholangiocarcinoma.     

化脓性肋软骨炎1例报告

病例女，25岁，2007年6月因重症急性胰腺炎、胰周脓肿在我院住院治疗，行B超引导下脓肿穿刺引流术。2007年7月出现胸骨柄及左侧肋骨区域剧烈疼痛，使用镇痛剂效果差，伴发热。查体：神志清楚，体温40℃、心率150次／min，血压134／69mmHg。上腹部饱满，剑突及左侧肋缘区域压痛。     

对宫颈锥切术后患者HPV认知及自我保护行为的调查

目的：了解宫颈锥切术后患者对人乳头瘤病毒（ HPV ）感染的认知及自我保护性行为状况。方法采用问卷调查方式对97例宫颈锥切术后患者HPV认知及自我保护性行为进行调查,分析调查结果并进行护理干预。3个月后进行随访,调查患者的依从性,采用配对样本t检验对干预前后患者对HPV保护性行为的态度进行检验。结果患者对HPV的主要传播途径及HPV是宫颈癌的危险因素的认知率较高,分别为100．0％和97．9％,患者对感染一种HPV类型意味着不会感染新的类型及HPV感染可以诱发阴道病变等问题认知严重不足,认知率分别为23．7％和5．2％。69．1％的患者手术治疗后不再坚持锻炼身体;91．8％的患者术后不坚持正确使用避孕套;72．2％的患者未能遵医嘱按时来院复查;91．8％的患者能坚持规律的生活方式。干预后坚持锻炼身体的患者占95．90％,严格使用避孕套的患者占97．90％,按时复诊的患者为100．00％,分别高于治疗前,差异有统计学意义（χ2值分别为13．33,28．90,15．77;P＜0．01）。结论应加强宫颈锥切术后患者自我保护性行为的宣教,以增强患者的自我保护意识。     

子宫切除术对女性情绪和生活质量的影响及护理对策

子宫切除术用于妇产科临床已有数百年历史,随着人类生活水平的提高及新医学模式的推进,人们越来越关注子宫切除对妇女性功能及生活质量的影响,一些专家主张将性功能、婚姻和生活质量提高这一目标确定为医学治疗疗效评定和最终结果[1],以致影响手术方式的抉择,但是一些改良的手术方式有它的局限性,无法取代传统的子宫切除术,临床上传统的全子宫切除术占主要比例。人是一个复杂的个体,手术可切去病变的子宫,但切不去患者的心理冲突和情绪障碍,不能改善患者的生活质量。如何能最大程度降低这些负面影响,改善患者术后生活质量,这已引起相关医务…     

川芎嗪预处理对大鼠肝缺血再灌注损伤能量代谢的保护作用

目的探讨川芎嗪预处理对大鼠肝脏缺血再灌注损伤(IRI)后肝脏能量代谢的影响。方法将120只雄性Wistar大鼠随机分为假手术组、实验组和对照组3组,实验组和对照组制作70%大鼠肝脏IRI模型,实验组大鼠在IRI前3d,每日腹腔注射川芎嗪注射液2mL,各组大鼠分别于术后1h、6h、24h、72h取肝组织观察组织形态学变化,检测肝细胞线粒体呼吸控制率(RCR)、磷氧比(P/O)和肝组织三磷酸腺苷(ATP)含量变化。结果①术后各个相应时相点实验组肝组织病理损伤均较对照组轻;②术后1h、6h、24h对照组肝细胞线粒体RCR及P/O均低于实验组(P均<0.05),72h均恢复正常(P>0.05);③实验组术后各相应时相点肝细胞ATP含量均高于对照组(P<0.05),并且恢复正常也比对照组较早。结论川芎嗪预处理能够改善肝脏能量代谢,提高缺血再灌注后肝脏的抗损伤能力。     

CT引导下经皮经胃胰头癌(125)~I粒子植入术疗效观察

胰腺癌是消化道恶性肿瘤的一种,发病率呈逐渐上升趋势,所以提供合理有效的治疗为患者后期生存时间的延长和生活质量的提高起着至关重要的作用,然而,胰头癌一经发现,大多为中晚期,切除率极低[1,2],治疗效果不佳。有报道通过胰头癌穿刺125I     

Effects of α1-adrenergic receptor on the proliferation of cholangiocarcinoma cells

Objective To investigate the correlation between α1-adrenergic receptor and the pathological behavior of cholangiocarcinoma, and the effects of norepinephrine (NE) on the proliferation of cholangiocarcinoma cell line QBC939. Methods Thirty-six samples of cholangiocarcinoma were resected in Southwest Hospital from August 2002 to March 2008. The expression of α1-adrenergic receptor in the 36 samples of cholangiocarcinoma tissue and 4 samples of normal bile duct tissue were detected by SABC technique. The proliferation of cholangio-carcinoma cell line QBC939 was detected after processing the cells with NE, phentolamine and prazosin. All the data were analyzed by chi-square test. Results The high positive expression rate of α1-adrenergic receptor was 68% (17/25) in patients with lymph node metastasis, which was significantly higher than 9% (1/11) in patients without lymph node metastasis (χ2=10.604, P<0.05). The high positive expression rate of α1-adrenergic receptor was 85% (11/13) in patients with middle and low positioned cholangiocarcinoma, which was significantly higher than 30% (7/23) in patients with hilar cholangiocarcinoma (χ2=9.753, P<0.05). NE promoted the proliferation of cholangiocarcinoma cell line QBC939 by stimulating the expression of α1-adrenergic receptor, and in a concentration-dependent manner. The proliferative effect was weakened as time passed by, and it was eliminated by phentolamine and prazosin. Conclusions The expression of α1-adrenergic receptor is diverse due to lymph node metastasis and the location of the tumor, α1-adrenergic receptor with high expression may play an important role in the proliferation and metastasis of cholangiocarcinoma.     

早期胸腔穿刺引流对重症急性胰腺炎急性肺损伤的影响

目的 探讨重症急性胰腺炎(SAP)伴发的少、中量胸腔积液早期穿刺引流对SAP患者急性肺损伤的影响。方法 回顾性分析中国人民解放军西部战区总医院 2015 年 1 月—2021 年 12月收治的107例 SAP 患者临床资料,根据入院后前3天是否进行胸腔穿刺置管引流,分为胸腔穿刺置管引流组(TPD组,51例)和非胸腔穿刺置管引流组(N-TPD组,56例)。分别比较入院后第5天、第10天两组相应时间节点实验室指标及临床结局。正态分布或近似正态分布的计量资料两组间比较采用成组t检验,非正态分布的计量资料两组间比较采用 Mann-Whitney U检验,计数资料两组间比较采用χ²检验。结果 TPD组重症监护病房住院时间、总住院时间、住院费用明显少于N-TPD组(P值均<0.05);而 TPD组与N-TPD组患者病死率(9.8%vs 14.3%,χ²=0.502,P=0.478)、脓毒症发生率(29.4%vs 44.6%,χ²=2.645,P=0.104)比较差异无统计学意义。TPD组急性呼吸窘迫综合征(ARDS)发生率明显...     

北京市丰台区孕妇妊娠期运动知识认知情况的调查

目的：了解丰台区孕妇对妊娠期运动的认知情况,通过护理干预后提高孕妇对妊娠期运动重要性的认知,增强孕妇的自我保健意识,为进一步指导孕妇孕期进行科学健康运动提供依据。方法采用便利抽样的方法,选取2013年6-7月在北京市丰台区妇幼保健院产科门诊建档的孕晚期（≥28周）孕妇117名,采用自行设计的妊娠期运动相关知识问卷对孕妇认知情况进行调查。结果117名孕妇妊娠期运动知识得分为0-15分,平均（8．99±1．41）分,平均正确认知率为60．3％;仅13．68％的孕妇经过正规的孕期运动相关知识培训,86．32％的孕妇表示没有参加过相关知识的培训。结论应重视低文化程度孕妇的培训,考虑孕妇的学习与接受能力,进行弹性教学,以取得更好的教学效果。     

Effects of α1-adrenergic receptor on the proliferation of cholangiocarcinoma cells

Objective To investigate the correlation between α1-adrenergic receptor and the pathological behavior of cholangiocarcinoma, and the effects of norepinephrine (NE) on the proliferation of cholangiocarcinoma cell line QBC939. Methods Thirty-six samples of cholangiocarcinoma were resected in Southwest Hospital from August 2002 to March 2008. The expression of α1-adrenergic receptor in the 36 samples of cholangiocarcinoma tissue and 4 samples of normal bile duct tissue were detected by SABC technique. The proliferation of cholangio-carcinoma cell line QBC939 was detected after processing the cells with NE, phentolamine and prazosin. All the data were analyzed by chi-square test. Results The high positive expression rate of α1-adrenergic receptor was 68% (17/25) in patients with lymph node metastasis, which was significantly higher than 9% (1/11) in patients without lymph node metastasis (χ2=10.604, P<0.05). The high positive expression rate of α1-adrenergic receptor was 85% (11/13) in patients with middle and low positioned cholangiocarcinoma, which was significantly higher than 30% (7/23) in patients with hilar cholangiocarcinoma (χ2=9.753, P<0.05). NE promoted the proliferation of cholangiocarcinoma cell line QBC939 by stimulating the expression of α1-adrenergic receptor, and in a concentration-dependent manner. The proliferative effect was weakened as time passed by, and it was eliminated by phentolamine and prazosin. Conclusions The expression of α1-adrenergic receptor is diverse due to lymph node metastasis and the location of the tumor, α1-adrenergic receptor with high expression may play an important role in the proliferation and metastasis of cholangiocarcinoma.