老年2型糖尿病患者胰高血糖素对血糖、血压变化的反应

The response of glueagon to the change of glucose and its relation to blood pressure in 71 eidely patients with type 2 diabetes was investigated. The results showed that the postprandial increment of glucagon in the group of patients with postprandial 2h plasma glucose increment<2.5 mmol/L was significantly higher than that in the group with plasma glucose inerement≥ 2.5 mmoi/L (P<0. 05). The postprandial increment of glucagon in patients with normal blood pressure was significantly higher than that in patients with hypertension (P<0.05). The results suggest that the decreased response of glucagon to the change in plasma glucose in elderly patients with type 2 diabetes is related to increased blood glucose and high blood pressure.     

老年2型糖尿病患者胰高血糖素对血糖、血压变化的反应

The response of glueagon to the change of glucose and its relation to blood pressure in 71 eidely patients with type 2 diabetes was investigated. The results showed that the postprandial increment of glucagon in the group of patients with postprandial 2h plasma glucose increment<2.5 mmol/L was significantly higher than that in the group with plasma glucose inerement≥ 2.5 mmoi/L (P<0. 05). The postprandial increment of glucagon in patients with normal blood pressure was significantly higher than that in patients with hypertension (P<0.05). The results suggest that the decreased response of glucagon to the change in plasma glucose in elderly patients with type 2 diabetes is related to increased blood glucose and high blood pressure.     

老年2型糖尿病患者胰高血糖素对血糖、血压变化的反应

The response of glueagon to the change of glucose and its relation to blood pressure in 71 eidely patients with type 2 diabetes was investigated. The results showed that the postprandial increment of glucagon in the group of patients with postprandial 2h plasma glucose increment<2.5 mmol/L was significantly higher than that in the group with plasma glucose inerement≥ 2.5 mmoi/L (P<0. 05). The postprandial increment of glucagon in patients with normal blood pressure was significantly higher than that in patients with hypertension (P<0.05). The results suggest that the decreased response of glucagon to the change in plasma glucose in elderly patients with type 2 diabetes is related to increased blood glucose and high blood pressure.     

阿司匹林对糖尿病大鼠肾脏COX-2表达的影响

目的探讨阿司匹林对糖尿病大鼠肾脏COX-2表达和病理的影响及其可能的作用机制。方法将24只SD大鼠随机分成3组,分别为正常对照(C)组、糖尿病(D)组[腹腔注射链酶佐菌素(STZ)60mg/kg]和糖尿病阿司匹林治疗(DT)组(先腹腔注射STZ 60mg/kg,第4天起给予0.9%氯化钠溶液溶解的阿司匹林1mg/kg.d灌胃)。8周后,观察3组肾脏组织在光镜、电镜下的病理表现,用免疫组化法和逆转录-聚合酶链式反应法观察COX-2的蛋白表达和COX-2mRNA的表达。结果 C组大鼠肾脏无明显病理变化;D组大鼠肾脏在光镜下可见细胞外基质增多,基底膜增厚,间质区可见炎性细胞浸润;DT组基质轻度增生,有少量炎性细胞浸润。在电镜下D组显示肾小球细胞基底膜不均匀,结构模糊,相邻足突大部分融合或缺失;DT组病理改变较D组减轻。D组大鼠COX-2蛋白表达较C组显著上调;DT组较D组显著下调。D组大鼠COX-2mRNA在肾脏的表达较C组显著上调(P<0.01),DT组较D组显著下调(P<0.01)。结论阿司匹林可以改善糖尿病大鼠肾脏的病理改变,其机制可能是通过抑制COX-2表达,改善由COX-2介导的病理改变。     

老年2型糖尿病患者胰高血糖素对血糖、血压变化的反应

The response of glueagon to the change of glucose and its relation to blood pressure in 71 eidely patients with type 2 diabetes was investigated. The results showed that the postprandial increment of glucagon in the group of patients with postprandial 2h plasma glucose increment<2.5 mmol/L was significantly higher than that in the group with plasma glucose inerement≥ 2.5 mmoi/L (P<0. 05). The postprandial increment of glucagon in patients with normal blood pressure was significantly higher than that in patients with hypertension (P<0.05). The results suggest that the decreased response of glucagon to the change in plasma glucose in elderly patients with type 2 diabetes is related to increased blood glucose and high blood pressure.     

GLP-1受体激动剂对2型糖尿病大鼠糖脂代谢和小肠L细胞功能的影响

目的研究胰高血糖素样肽1（GLP-1）受体激动剂对2型糖尿病大鼠小肠L细胞GLP-1表达的影响。方法观察正常大鼠和2型糖尿病大鼠经GLP-1受体激动剂Exenatide干预前后体质量、空腹血糖、血脂谱、空腹胰岛素、胰岛素抵抗指数和胰岛素敏感指数的变化,免疫组织化学方法检测大鼠小肠L细胞GLP-1的表达变化。结果与糖尿病组比较,糖尿病Exenatide干预组大鼠的体质量、空腹血糖、空腹胰岛素、血脂谱、胰岛素抵抗指均有明显改善,差异有统计学意义（P〈0．05）。糖尿病组和糖尿病Exenatide干预组大鼠小肠L细胞GLP-1的表达均显著低于正常对照组（P〈0．0l,P〈0．05）;与糖尿病组比较,糖尿病Exenatide干预组大鼠小肠L细胞GLP-1的表达显著升高（P〈0．05）。结论GLP-1受体激动剂可能对改善糖尿病大鼠小肠I．细胞GLP-1表达功能有-定作用。     

人工抗原提呈细胞的制备和应用的研究

目的 制备人工抗原提呈细胞（artificial antigen presenting cell,aAPC）并用它从HLA-A2阳性健康个体外周血单个核细胞（PBMC）中诱导和扩增特异性细胞毒性T淋巴细胞（CTL）.方法 将HLA-A2-EBV四聚体分子和抗CD28抗体分子吸附固定在细胞大小的聚苯乙烯乳胶微球（5μm）表面制成aAPC;采用流式细胞仪表型分析;aAPC和HLA-A2阳性个体人外周血单核细胞进行混合淋巴细胞反应;用HLA-A＊0201-EBV四聚体染色法检测特异性CTL的频率;应用细胞内细胞因子染色法检测特异性CTL功能性细胞因子IFN-γ的分泌;采用LDH释放法检测特异性CTL的特异杀伤活性.结果 流式细胞仪分析显示微球表面吸附有HLA-A2-EBV四聚体分子和抗CD28抗体分子;四聚体检测及细胞内细胞因子染色法检测与经典细胞毒试验结果一致,结果表明aAPC在体外可诱导抗原特异性CTL的生成.结论 aAPC制备成功,并在体外有效地诱导抗原特异性CTL的生成.     

华东地区自然人群血清维生素D水平调查

正中华内分泌代谢杂志,2017,33(9):726-729.该文了解中国华东地区自然人群的维生素D水平,为该地区人群预防维生素D缺乏及其相关疾病提供依据。方法:数据来源于2014年中国华东地区代谢性疾病的患病率及危险因素的横断面研究数据库,年龄≥18岁目前居住于此区域或居住时间≥6个月的成年人12 662名纳入此项研究。以血清25-羟维生素D     

腹型肥胖与2型糖尿病患者胰高血糖素关系的研究

T2DM组67例(分为非腹型肥胖组、腹型肥胖组、肥胖组)及正常对照组19例,测量FPG、h2PG、CP、GG。结果T2DM患者餐后C肽、GG显著升高[(4.92±4.01vs2.11±0.67)ng/mL,(201.22±46.66vs125.23±40.35)pg/mL,均P0.01];与非腹型肥胖组相比,腹型肥胖组的T2DM患者餐后C肽及其增加值显著升高[(6.65±4.62vs3.46±2.22)ng/mL,(5.07±4.19vs2.21±1.81)ng/mL,均P0.05],餐后胰高血糖素及其增加值相对升高但未达到统计学意义[(204.35±43.76vs203.06±51.27)pg/mL,(25.16±36.20vs16.04±19.14)pg/mL,均P0.05];与腹型肥胖组相比,肥胖组餐后C肽、GG及相应增加值升高,但未达到统计学意义。结论T2DM患者的胰岛α细胞存在胰岛素抵抗,腹型肥胖者胰岛α细胞的胰岛素抵抗更显著。     

不同品系大鼠肝脏卵圆细胞增殖模型的比较研究

目的 对不同品系大鼠肝卵圆细胞增殖模型进行比较,筛选建模效率较高的大鼠品系.方法 选择F344、Wistar和SD大鼠各10只,经2-乙酰氨基芴灌胃和四氯化碳腹腔注射建立肝脏卵圆细胞增殖模型,组织学和免疫组织化学检测鉴定.荧光激活细胞筛选法分选并纯化各品系大鼠肝脏卵圆细胞,分析Thy-1.1~＋细胞百分率,观察Thy-1.1~＋细胞培养结果.结果 成功建立三种品系大鼠肝脏卵圆细胞增殖模型.流式细胞仪分选结果显示,F344、wistar和SD大鼠Thy-1.1~＋细胞百分率分别为（9.46±0.99）%、（2.46±0.37）%和（1.46±0.12）%;各品系大鼠间Thy-1.1~＋细胞百分率比较,差异有统计学意义（P〈0.05）.细胞培养观察发现,与Wistar和SD大鼠比较,F344大鼠分选得到的肝脏卵圆细胞生长状态好且纯度较高.结论 在用于建立肝脏卵圆细胞增殖模型最为常见的三种品系大鼠中,F344大鼠所建模型的肝脏卵圆细胞活化效率最高.