基于网络药理学方法赤芍-牡丹皮药对治疗中风作用机制探讨

目的:采用网络药理学的分析方法研究赤芍-牡丹皮经典药对治疗中风的分子作用机制。方法:基于中药系统药理数据库和分析平台(TCMSP)、药物与疾病数据库(Drug Bank)、赤芍-牡丹皮的疾病与化学成分靶点网络则分别由治疗靶标数据库(TTD)建立,而它的药效成分与靶点网络主要运用Cytoscape 3.7.0软件构建而成,并同时进行了网络拓补分析; 至于核心靶点对基因本体(GO)的功能富集则主要运用DAVID数据库完成,同时也对百科全书KEGG(基因组)通路的功能富集进行了研究。结果:网络拓补分析结果表明,最核心的药效成分可能为槲皮素、山奈酚、鞣花酸、黄芩苷,最核心的GO功能有活性氧代谢过程、脂多糖、核受体活性、细胞因子受体结合、细胞膜微区、囊腔作用等; 最核心的作用通路有VERF通路、Toll样受体通路、肿瘤坏死因子(TNF)通路、白细胞介素-17(IL-17)信号通路、磷脂酰肌醇3激酶-蛋白激酶b(P13K-Akt)通路等。结论:本文基于网络药理学方法对赤芍-牡丹皮药对干预中风的作用机制进行了分析发现,赤芍-牡丹皮药对可能是通过VERF通路、Toll样受体通路、TNF通路、IL-17信号通路、P13K-Akt等通路协同作用发挥治疗缓解中风功效,上述预测结果可为赤芍-牡丹皮药对在中风治疗的药理作用研究及临床应用提供一定的理论依据。     

基于整合药理学的当归抗动脉粥样硬化的作用机制研究

目的基于整合药理学,研究当归抗动脉粥样硬化(Atherosderosis,AS)的作用机制。方法借助中药整合药理学平台(TCMIP V2.0),预测分析当归抗动脉粥样硬化的机制及其药效物质基础。结果当归抗动脉粥样硬化的17种有效成分,直接或间接作用于PPARG(过氧化酶体增殖物激活受体)、APOE(载脂蛋白E)、卵磷脂胆固醇酰基转移酶(LCAT)、炎性趋化因子(CCL2)、基质金属蛋白酶9(MMP9)、MTTP(微粒体三酰甘油转移蛋白)等26个核心药靶,这些药靶作用涉及胆固醇跨膜转运、胆固醇酯代谢、炎症反应、脂质氧化修饰、PPARα活性基因的表达等10条主要调控通路。结论可预测当归中部分有机酸和挥发油成分主要通过PPARG、MTTP靶标作用炎症及脂质代谢通路对抗AS的发生。     

基于区块链的中医药供应链信息共享机制研究

阐述区块链技术在中医药供应链管理模式方面的应用价值和影响,分析目前中医药供应链系统业务信息共享链条存在的问题,提出基于区块链技术的信息共享机制解决方案,促进区块链在供应链管理体系中的实践应用,同时为中医药、区块链、供应链交叉领域的理论研究提供新思路。     

Antiproliferative role of Indigofera aspalathoides on 20 methylcholanthrene induced fibrosarcoma in rats

ObjectiveTo find out the anticancer effect of Indigofera aspalathoides (I. aspalathoides) on 20-methylcholanthrene induced fibrosarcoma in rats.MethodsFibrosarcoma was induced in Wistar strain male albino rats by 20-methylcholanthrene. Intraperitoneous (i.p.) administration of 250 mg/kg body weight/day of aqueous extract of I. aspalathoides for 30 d effectively suppressed chemically induced tumors. Parameters such as body weight, liver and kidney weight, tumor weight, mean survival time, behavioral changes, blood glucose, blood glycogen and marker enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP) and 5′-nucleiotidase (5′-NT) in serum, liver and kidney and lipid profiles such as total cholesterol, phospholipids, free fatty acids in liver and kidney of control and experimental animals were studied.ResultsFibrosarcoma bearing animals were ferocious and anxious. The mean survival time was found to increase after the treatment. The body weights were significantly decreased (P < 0.001) in group II fibrosarcoma animals which steadily increased after the treatment with I. aspalathoides. The liver and kidney weights were significantly increased whereas the tumor weights decreased as compared to the weights in untreated fibrosarcoma bearing rats. The blood glucose and the liver and kidney glycogen levels were found to decrease significantly (P < 0.001) in group II animals. Elevated activities of marker enzymes were observed in serum, liver and kidney of fibrosarcoma bearing Group II animals which were normalize after I. aspalathoides treatment. In the liver and kidney of Group II animals the total cholesterol increased whereas the phospholipids and free fatty acid levels decreased (P < 0.001) which were normalized after treatment.ConclusionsThe treatment by I. aspalathoides on fibrosarcoma bearing rats has improved the levels of various parameters indicating its antiproliferative and anticancer activity.     

光叶丁公藤中化学成分的UPLC-Q-Exactive Focus-MS/MS鉴定

目的:利用UPLC-Q-Exactive Focus-MS/MS技术对光叶丁公藤中的化学成分进行快速识别和鉴定。方法:选用80%甲醇提取光叶丁公藤作为供试品溶液,对光叶丁公藤药材的化学成分进行分析和鉴定。采用Thermo Accucore aQ C_(18)色谱柱(2.1 mm×150 mm,2.6μm)进行色谱分离,以甲醇(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱(0～12 min,5%～25%A;12～20 min,25%～30%A;20～28 min,30%～38%A;28～40 min,38%～42%A);质谱分析采用正、负离子监测模式和加热电喷雾离子源(HESI),扫描范围m/z 80～1 200。结果:共鉴定出了42个化学成分,包括香豆素类12个、绿原酸类14个、生物碱类1个、酰胺类1个,其余14个为酯化糖苷类。结论:UPLC-Q-Exactive Focus-MS/MS技术可以快速、全面地鉴定光叶丁公藤药材中的化学成分,其中11个成分经与对照品比对后准确鉴定,31个成分在该植物中属首次发现,2个成分在丁公藤属植物中属首次发现,可为光叶丁公藤的物质基础研究和替代品开发提供重要依据。     

西安市学龄前儿童气质类型现况调查

目的 分析西安市学龄前儿童气质类型的分布特点,为家长、幼教工作者采取科学的育儿方法提供参考依据。方法 于2014年10月采用随机整群抽样的方法选择西安市东、西、南、北4个郊区和城区的幼儿园各1所,每个幼儿园大、中、小班各抽取30%~50%班级共1 111名儿童,在父母或监护人知情同意后进行问卷匿名填写。问卷包括一般情况问卷、3~7岁儿童气质量表两部分。结果 1)西安市学龄前儿童气质类型的分布:平易(E)型、中间偏易(I-E)型、发动缓慢(S)型、中间偏烦(I-D)型、麻烦(D)型分别为38.52%、36.91%、12.51%、8.46%、3.60%;个性分布以E+ I-E为主(>70%),D+ I-D最少(<13%)。2)各年龄段气质类型的分布差异无统计学意义(P均＞0.05);S型女童高于男童(P < 0.01)。3)与常模及北京市儿童比较,差异无统计学意义(P均＞0.05);而与上海、广州比较,中间型、D型的百分率低(P均<0.05)、E型高(P均< 0.01)。各年龄段按性别分层分析后显示:4岁女童S型百分率高于男童(15.60% vs.8.57%,P=0.05);5岁女童D型高于男童(6.89% vs.1.25%,P<0.05),而I-E型低于男童(25.12% vs.38.75%,P<0.05)。结论 西安市学龄前儿童气质类型以E+I-E型为主。气质类型的分布受性别、地域的影响,而与年龄无关。家长及教育工作者应该按照其气质类型采取适当的教育方法,促进儿童健康成长。     

龙生蛭胶囊治疗缺血性脑中风的临床应用进展

本文概述了缺血性脑中风的致病原因及中医治疗原则,介绍了龙生蛭胶囊的处方组成、功能主治,综述了龙生蛭胶囊近年来治疗缺血性脑中风的临床研究。结果均显示龙生蛭胶囊在治疗缺血性脑中风中疗效显著,可以长期应用于临床治疗。本文可对龙生蛭胶囊的基础研究和临床应用提供一定的依据。     

Systems pharmacology dissection of the anti-stroke mechanism for the Chinese traditional medicine Xing-Nao-Jing

Xing-Nao-Jing (XNJ) is a well-known injection that has been extensively applied in clinical treatment of stroke in China. However, the underlying mechanism of clinical administration of XNJ in stroke remains unclear. In this study, a systems pharmacology strategy based on pharmacokinetic and pharmacodynamics data was applied to analyze the pharmacological effect of XNJ on stroke. Sixteen active compounds were filtered from XNJ through Drug-likeness (DL) and Brain-blood-barrier (BBB) evaluations. Ninety-four potential targets of these active components were identified by SysDT and SEA. Biological process and pathway enrichment analyses of these targets demonstrated that XNJ exerted anti-stroke effects by biological processes and pathways, such as the response to oxidative stress, regulation of blood pressure, calcium signaling pathway, and apoptosis. Integrating the compound-target network and stroke-related PPI network, we found that Akt1, HIF-1α and ITGB2 may play key roles in the treatment of stroke. The experiments demonstrated that oxycurcumenol may prevent PC12 cells from oxidative stress-induced cell damage. Our study indicates that XNJ has an effect on stroke by protecting neuro cells from oxidative stress-induced cell damage via HIF1α, and the research strategy at the systems pharmacology level is feasible to reveal the mechanisms of novel lead compounds from natural products.     

脉抒降脂及改善急性酒精性肝损伤的作用

目的:研究脉抒降脂及对急性酒精性肝损伤大鼠的保护作用。方法:采用高脂饲料喂养建立高脂动物模型,将雄性SD大鼠随机分为6组,即对照组、模型组、阳性给药组、脉抒高、中、低剂量组;除对照组外其余各组大鼠高脂喂养同时给药,4周后测定血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及丙二醛(MDA)、超氧化物歧化酶(SOD)水平。另取大鼠60只,分为对照组、模型组、硫普罗宁组及脉抒高、中、低剂量组,用白酒灌胃诱导、建立大鼠酒精性脂肪肝模型,各组动物连续灌胃给药28 d,测定血清中TG、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平,ELISA法测定血清极低密度脂蛋白(VLDL)水平;进行肝组织病理学检查。结果:和模型比较,脉抒高、中、低剂量组均对高脂大鼠体重未见明显影响;对高脂大鼠血清TC和HDL-C水平无明显影响;但可显著性降低高脂大鼠血清TG和LDL-C水平。同时能够升高大鼠血液中SOD水平,降低MDA水平(P0.05,P0.01)。可明显降低急性酒精性肝损伤大鼠血清ALT、AST、TG和VLDL水平,脉抒大剂量可改善急性酒精性肝损伤大鼠肝脏脂肪变性程度(P0.05,P0.01)。结论:脉抒对高脂大鼠有明显的辅助降血脂作用同时对急性酒精性肝损伤大鼠有明显的辅助保护作用。