两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

洪涝灾害救援护理人员胜任力指标体系的构建

目的构建洪涝灾害救援护理人员胜任力指标体系。方法通过文献调研、理论研究、半结构式访谈法初步拟定洪涝灾害救援护理人员胜任力指标体系;运用Delphi专家咨询法对32名专家进行2轮咨询,并运用层次分析法和专家重要性赋值平均分配法确定各指标权重。结果构建的洪涝灾害救援护理人员胜任力指标体系包括3个一级指标、12个二级指标和77个三级指标。专家权威系数0.84,两轮咨询变异系数分别为9.20%~21.60%和0.00%~19.37%,协调系数分别为0.200-0.317和0.221-0.361,具有统计学差异(P0.05)。结论本研究构建的洪涝灾害救援护理人员胜任力指标体系突出了我国洪涝灾害救援的特点,为洪涝灾害救援护理人员的选拔、培训及考核提供参考依据。     

激光技术在口腔疾病治疗与口腔修复领域的应用研究进展

口腔疾病的常规治疗,如根管治疗、根面平整治疗等传统方法,存在着消毒深度不足、术后疼痛明显、牙根清创程度有限等问题。同时,在口腔修复体的制造中,传统的失蜡法存在着工序复杂、产品内部微缺陷较多、成形件服役寿命不足的问题。尽管数字化铣削法能够保证修复体的力学性能,但材料浪费与刀具磨损却影响到了该方法的普适性。随着激光技术在口腔医学领域的不断发展,更多的研究人员不仅将该技术用于辅助口腔疾病的临床治疗,而且也引入到修复体的制造中。整体上,激光技术比传统方法更具有优势。本文对激光技术在口腔疾病（牙体、牙髓、牙周疾病）治疗和口腔修复领域中的应用进行综合性分析、讨论与展望作一综述,以期为深入了解该技术的优缺点及适用性提供科学依据。     

通过2006年度全国优秀院长业绩分析看中国医院管理趋势

通过对2006年度“全国优秀院长”业绩材料进行分析,研究发现当代院长们在医院管理中更加注重加强社会效益、树立创新理念、发挥品牌效应、实现医研教综合发展、提高医疗含金量和实现人性化管理。同时也发现在医院进一步发展中有待提高对集约式发展、医疗安全、数字化革命和专业化管理的关注与投入。     

高血压脑出血120例急性期与康复期护理体会

2004年8月～2006年6月，我们共收治高血压脑出血患者120例，经精心护理，效果满意。现将急性期与康复期护理体会报告如下。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

3—10孕月龄胎儿体重的指数曲线模型

<正> 近年,国内对胎儿各体表指标及内脏器官在各个不同孕龄间的生长情况做了大量的研究及交流。对胎儿体重与孕龄间依存关系的研究均用直线回归方法。国外资料曾用指数方程来分析胎儿身高与体重的关系。目前采用曲线回归分析研究体重与孕龄间的关     

患者输血前试验血液标本拒收原因分析

目的 探讨输血前试验血液标本的影响因素.方法 对来源于不同医疗机构的血液标本的拒收原因进行分析.结果 2006年4月～2010年12月,本站共拒收血液标本252份（0.85％）,分为12项影响因素：检测申请关键信息缺失或不符的34份,占13.49％;标本管上无标识或标识不清、不正确共52份,占20.63％;标本管不符合要求26例,占10.32％;标本量不足或被稀释等140份,占55.56％.结论 按临床输血质量管理体系要求,制定和执行血液标本规范化管理制度,才能提高输血前试验标本质量.     

负压采血试管在血型鉴定方面的选择和应用

目的探讨负压采血试管在血型鉴定方面的选择应用方案。方法采用不同类别的负压采血试管分组采集1 018例血液标本分别进行ABO和Rh血型鉴定。结果枸橼酸钠、EDTA-K2、肝素钠试管采集的抗凝血标本,分别以红细胞进行ABO正定型和RhD鉴定无影响,以血浆进行ABO反定型,对结果影响比例分别为28.9%(178/616)、34.9%(357/1 018)、35.1%(141/402);普通管、促凝管采集的不抗凝血标本,分别以凝血块洗下红细胞进行ABO正定型和RhD鉴定,ABO正定型结果影响比例分别为41.2%(254/616)、32.1%(129/402),对RhD鉴定的影响被RhD阳性结果掩盖。以普通管、促凝管血清分别进行ABO反定型,普通管由于红细胞凝集挂壁处理过程引起溶血改变占4.5%(28/616),干扰ABO反定型结果,促凝管无影响。结论以任1种负压试管单采1管标本均影响血型准确鉴定,宜同时选择抗凝和不抗凝2管采集标本分别应用于ABO正定型、RhD鉴定和ABO反定型,其中首选EDTA-K2和促凝管为最佳。     

轻型车载野战医院的研制

围绕如何展开“处突、反恐、维稳、救援”医疗保障任务,武警总医院研制了卫勤指挥车、外科手术车、综合急救车、医技检查车、留观救治车、前接后送车、医疗物资保障车、后勤生活保障车为一体的车载野战医院,能达到机动性强、配比合理、救治功能齐备、操作方便等特点。