类脂质渐进性坏死1例

报告类脂质渐进性坏死1例。患者女,68岁,因右下肢胫前斑块2年就诊。皮肤专科检查:右下肢胫前可见一大小约10 cm×3 cm黄棕色斑块,边界清楚,表面光滑有光泽,可见毛细血管扩张和褐色斑,上覆鳞屑,上下端皮损中央萎缩凹陷,上见痂,质硬,无压痛。皮损组织病理检查:表皮轻度萎缩,真皮见胶原变性灶,周围组织细胞呈栅栏状排列,真皮浅深层毛细血管增生,管壁增厚,血管周围淋巴细胞、浆细胞、组织细胞及多核巨细胞浸润。实验室检查:血、尿、大便常规、肝肾功能正常,口服葡萄糖耐量试验提示糖耐量异常,糖化血红蛋白正常。诊断:类脂质渐进性坏死。予铒点阵激光(2 940 nm)联合卤米松/三氯生乳膏外用,皮损较前消退,目前仍在随访中。     

从“少阴有余”论治胆碱能性荨麻疹

以"少阴有余"理论为指导,结合胆碱能性荨麻疹的现代发病机制,探讨其中医病因病机及治法方药。认为本病的基本病机为少阴之心火有余或肾水不足致心火亢盛,一者乘肺克金,二者灼伤脉络,传于皮肤,则为瘾疹。中医诊治本病,一方面要围绕心火亢盛展开,另一方面要重视调摄心神。治法以清心安神或交通心肾为主,辅以心理疏导。     

强脉冲光联合他克莫司软膏治疗面部脂溢性皮炎疗效观察

目的探讨强脉冲光联合他克莫司软膏治疗面部脂溢性皮炎的临床疗效。方法纳入面部脂溢性皮炎患者60例,随机分为试验组和对照组。试验组予以强脉冲光联合他克莫司软膏治疗,对照组予以他克莫司软膏治疗,疗程共8周,治疗结束后评估疗效及不良反应。结果试验组临床症状改善明显优于对照组,试验组有效率更高(64.52%vs.34.48%),差异有统计学意义(P0.05)。两组均未见明显不良反应。结论强脉冲光联合他克莫司软膏治疗面部脂溢性皮炎安全有效。     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

复方二藤洗剂治疗掌跖脓疱病疗效观察

目的:观察清热解毒类中药治疗掌跖脓疱病的临床疗效。方法:采用复方二藤洗剂(南蛇藤、忍冬藤、三桠苦、大飞杨、黑面神、虎杖等)外洗治疗本病38例,并设对照组,观察两组临床症状改善情况。结果:治疗组总有效率为86.84%,对照组总有效45.16%,差异有统计学意义(P<0.01)。结论:本方法对本病有清热解毒,祛班消疱的功效。     

补肾生发方联合非那雄胺治疗脂溢性脱发临床疗效分析

目的观察补肾生发方联合非那雄胺治疗脂溢性脱发的临床疗效。方法将127例患者随机分为两组,治疗组63例给予补肾生发方联合非那雄胺片治疗,对照组64例给予六味地黄丸和非那雄胺片治疗。结果治疗组显著改善13例,中度改善19例,轻度改善27例,无变化4例,总有效率93.65%;对照组显著改善9例,中度改善12例,轻度改善24例,无变化19例,总有效率70.31%,两组总有效率比较差异有统计学意义(P0.05)。对治疗前后两组头发油腻程度、头皮屑数量以及头痒等伴随症状改善情况进行比较,治疗组均明显优于对照组,差异有统计学意义(P0.05)。结论补肾生发方联合非那雄胺治疗脂溢性脱发在临床疗效及伴随症状改善方面均明显优于对照组,差异有统计学意义,值得临床推广。     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

皮肤支气管源性囊肿1例

 报告成人皮肤支气管源性囊肿1例。患者男，22岁，左颈部皮疹3年。皮肤科检查：左颈部皮肤见一约0.2 cm×0.2 cm大小的淡红色丘疹，边界清楚，中间可见破口，未见明显液体流出。皮肤镜检查：非偏振光下淡红色背景，可见湿润性破损口；偏振光下淡红色背景，镜下见中央破损口，外周红白色无结构区，绕以扩张的分支状血管。皮损组织病理学检查：真皮层见一角囊肿及一腺腔，腺腔内衬假复层纤毛柱状上皮，可见个别粘液细胞。腺腔周围见淋巴细胞浸润。真皮上方表皮呈息肉样突起，表皮鳞状上皮组织局部与复层柱状上皮组织相移行。诊断：皮肤支气管源性囊肿。外科手术切除后随访3个月，未见复发。     

基于网络药理学-分子对接探讨益气养阴通络方抗类风湿关节炎的作用机制

[目的] 采用网络药理学与分子对接探究益气养阴通络方干预类风湿关节炎（RA）的作用机制。[方法] 通过中药系统药理学数据库及分析平台（TCMSP）、本草组鉴（HERB）和文献查阅获得复方活性成分与靶点,采用Genecards、在线人类孟德尔遗传数据库（OMIM）等数据库获得疾病靶点,然后采用数据库计算出益气养阴通络复方成分与疾病的交集靶点。对交集靶点施行功能和通路富集分析,选取益气养阴通络方核心药物靶点与关键化合物施行分子对接。[结果] 网络药理学共筛选出益气养阴通络方的 83种活性成分和779个潜在靶点,RA相关靶点5 393个,交集靶点465个,其中交集核心靶点9个（分别为SRC、STAT3、MAPK3、MAPK1、HSP90AA1、PIK3R1、GRB2、AKT1、PIK3CA）。富集得出PI3K-AKT、癌症相关及神经活性配体-受体相互作用等通路,其中PI3K-AKT信号通路可能是复方干预RA的关键通路之一。对接计算表明,益气养阴通络方的11种主要活性成分与关键靶点SRC、STAT3、PIK3R1、AKT1均有较稳定的结合,其中与AKT的结合可能最大。[结论] 益气养阴通络方通过多成分-靶点-通路的方式干预RA,PI3K-AKT、癌症相关及神经活性配体-受体相互作用通路均为可能的作用通路。