p62基因对黑素瘤A375细胞生物学特性的影响

目的研究p62基因在黑素瘤A375细胞生长增殖及细胞周期调控等方面的作用。方法取对数生长期的A375细胞,用干扰p62基因表达的siRNA慢病毒载体转染细胞;MTT法检测转染及正常A375细胞的平均OD值;流式细胞术检测两组的细胞周期分布。结果转染组的平均OD值较正常组小(P0.05);转染组的G0/G1期比例(0.73±0.01)较正常组(0.64±0.02)高(P=0.002);转染组的凋亡率(0.10±0.02)较正常组(0.03±0.01)高(P=0.004)。结论干扰p62表达后,细胞周期阻滞于G0/G1期,p62在维持细胞周期和肿瘤细胞凋亡中起着重要的作用。     

果酸治疗痤疮的效果观察

目的 观察评价果酸[甘醇酸(glycolic acid,GA)]治疗痤疮及痤疮瘢痕的临床疗效,探讨果酸治疗的适应证、方法及安全性.方法 34例痤疮患者,应用浓度为20％、35％、50％、70％的羟基乙酸,进行化学性剥脱治疗,每3周1次,共治疗5次,通过比较治疗前后皮损计数进行疗效观察,观察项目包括:治疗前后毛孔大小、油脂分泌情况、皮肤光滑度、白皙度、瘢痕大小及深浅度,GA治疗对工作生活的影响及疗效满意程度等,并做出疗效评价.结果 34例患者均按计划完成治疗.痊愈4例,显效15例,有效12例,无效3例,总有效率91.18％.治疗后73.53％(25/34)患者毛孔 改善,55.88％(19/34)患者油脂分泌减少,82.35％(28/34)患者皮肤光滑度提高,61.77％(21/34)患者肤色均匀白皙,67.65％(23/34)瘢痕改善.结论 GA适合用于治疗轻、中度痤疮,并能改善毛孔粗大,减少油脂分泌,提高皮肤光滑度,使肤色均匀白皙,减轻痤疮瘢痕,安全且无严重不良反应,患者易于接受,并得较好的评价.     

痤疮患者血清IL-10水平的检测及其意义

目的:了解痤疮患者血清IL-10的水平,分析其与痤疮患者临床特征的相关性,探讨IL-10在痤疮中的临床意义。方法:选取于我院门诊就诊的60例痤疮患者(按Pillsbury分为Ⅰ~Ⅳ级,每级15例,共4组)作为研究组,同时选取无痤疮健康体检者15例作为对照组,采用人白介素10(IL-10)酶联免疫分析试剂盒测定五组血清的IL-10水平并进行比较。结果:研究组血清IL-10水平与年龄及性别均无相关性(P值均>0.05);研究组血清IL-10水平均高于对照组,差异均有统计学意义(P值均<0.05);而研究组各组血清IL-10水平间差异无统计学意义(P值均>0.05)。结论:痤疮患者血清IL-10水平较正常健康人高,IL-10可能参与了痤疮的发病机制但不能用来评价痤疮的病情严重程度。     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

痤疮患者治疗情况临床流行病学分析

目的　了解痤疮患者目前的治疗状况和就医行为特点。方法　对 2 3 3 1例门诊痤疮患者进行问卷式流行病学调查。结果　在近 1年内接受过治疗的痤疮患者占所调查痤疮患者总数的 5 2 .6% ;痤疮程度重、女性患者和广州患者更愿意接受治疗 ;患者对治疗满意的占 43 % ,Ⅲ级和Ⅳ级痤疮患者更容易对治疗不满意 ,而在医院接受治疗、采用维持治疗和同时配合面膜治疗的患者一般对疗效满意。结论　要对痤疮患者进行健康教育 ,及早、正确、足够疗程的治疗和维持治疗对于提高疗效和患者的满意度非常关键     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

表皮肿瘤中COX-2与P53的检测

目的了解表皮肿瘤组织环氧合酶-2(COX-2)与P53的表达情况。方法免疫组化法检测脂溢性角化病(SK)15例、Bowen’s病(BD)15例、基底细胞上皮瘤(BCE)20例、鳞癌(SCC)20例COX-2和P53的表达。结果所检测的各肿瘤组织标本均有COX-2的表达,分别为SCC95%,BD73.3%,SK46.6%,BCE68.0%,表达强度以SCC最为显著,周围正常组织未见表达。突变型P53在SCC(80.0%),BCE(75.0%),BD(33.3%)肿瘤组织的阳性率较高,而在SK(13.3%)中基本不表达。SCC和BCECOX-2表达阳性者其突变型P53表达的阳性率较COX-2表达阴性者高(P<0.05)。结论表皮肿瘤存在COX-2的过表达;P53的突变与COX-2的过表达有关,p53突变可能是通过上调COX-2水平发挥抗凋亡作用,从而促进SCC,BCE的形成和发展。     

寻常痤疮严重程度和痤疮瘢痕相关因素分析

目的：了解门诊痤疮患者严重程度的相关因素及导致出现痤疮瘢痕的危险因素。方法：对2331例门诊痤疮患者进行问卷调查。结果：14～19岁、居住在农村、初中文化程度、油性皮肤、伴发脂溢性皮炎、每天睡眠时间少于8h的患者痤疮病情更严重。男性、有瘢痕家族史、痤疮程度严重的患者出现痤疮瘢痕的概率比较大。结论：对于具备重度痤疮高危因素的患者，应及早干预，给予积极的治疗。由于重度痤疮出现瘢痕的概率更高，因此早期、及时的治疗对于避免出现瘢痕也有一定帮助。     

小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.     

中药狼疮Ⅱ号胶囊辅助治疗对活动期SLE患者疗效的影响及血液系统指标关系的初探

目的:观察狼疮Ⅱ号结合激素治疗对中度活动期阴虚内热证SLE患者SLEDAI积分的影响,并进行血液学指标与SLEDAI积分之间的相关性分析,探讨狼疮Ⅱ号对活动期SLE患者血液学指标的影响。方法:两组均予以激素治疗,同时治疗组给予中药制剂狼疮Ⅱ号,对照组予以安慰剂治疗。结果:1两组治疗后SLEDAI积分均有下降,差异有统计学意义(P<0.05);2两组SLEDAI积分减少比较,差异有统计学意义(P<0.05);组间SLEDAI积分差值比较,两组差异有统计学意义(P<0.05);3SLEDAI积分与血液中相关指标进行相关及回归分析,均未发现与两种治疗方法分别对应的相关敏感性指标。结论:分别进行两种治疗方法与血液中相关指标相关及回归分析,未发现与两种治疗方法对应的敏感指标,这可能和血液相关指标在SLEDAI评分体系中所占权重较低有关。