腹腔镜胆囊切除术中腹腔引流管的应用体会

目的：总结腹腔镜胆囊切除术（laparoscopic cholecystectomy,LC）中腹腔引流管的临床应用经验。方法：回顾分析2001年1月至2006年6月1650例LC患者的临床资料。结果：1650例中1487例（90.1%）术中未放置腹腔引流管,恢复顺利,放置腹腔引流管的163例中159例（97.5%）引流管无明显液体引出,或仅有少量腹腔冲洗液,术后24～48h拔除,康复好,4例患者术后发生胆漏。结论：应严格掌握放置腹腔引流管的指征,多数LC术中不放置腹腔引流管是合理可行的,更有利于患者的康复。     

卒中后认知障碍患者粪便钙卫蛋白测定及其与血清炎性因子的相关性

目的： 探讨卒中后认知障碍（post stroke cognitive impairment,PSCI）患者粪便钙卫蛋白（fecal calprotectin,FC）水平及其与认知状态和血清炎性因子的相关性。方法: 收集2021年3月至2022年3月就诊的PSCI患者32例、卒中后无认知障碍（post stroke with no cognitive impairment,PSNCI）患者44例和无卒中对照者25例,检测并对比3组中FC水平以及PSCI组治疗前后的FC水平。对PSCI组进行简易智力状态检查量表（mini mental state examination,MMSE）和蒙特利尔认知评估量表（Montreal cognitive assessment,MoCA）评估,并检测血清C 反应蛋白（CRP）和白介素 6（IL 6）的水平。结果: PSCI组、PSNCI组和对照组的年龄、性别、吸烟史、饮酒史未见明显差异,高血压史和糖尿病史在3组间存在显著差异（P<0.05）,PSCI组FC水平显著高于PSNCI组和对照组（P<0.01）。对PSCI患者改善认知治疗1个月后,PSCI患者FC水平较治疗前显著降低（P<0.01）。在PSCI组中,FC水平与MoCA评分和MMSE评分呈负相关（P<0.01）,与血清CRP水平和血清IL 6水平呈正相关（P<0.01）。结论: PSCI患者FC水平升高,与PSCI患者的认知功能和血清炎性因子具有相关性,在PSCI的发生机制中发挥重要作用。     

食管癌术后锁骨上和纵隔淋巴结转移放射治疗的疗效评价

 目的　评价食管癌术后锁骨上和纵隔淋巴结转移局部放射治疗的意义。方法　回顾性分析1998年 7月～ 2 0 0 0年 2月间收治的 5 7例食管癌术后锁骨上和纵隔淋巴结转移患者 ,局部放射治疗 ,常规分割放疗剂量 5 0～ 70Gy。结果　 82 .4 %左右的肿瘤得到局部控制 ,1、2、3年生存率分别为 5 6 .1%、2 9 .8%、10 .5 % ,72 .5 %的病人死于野外转移。结论　转移淋巴结≤ 3cm者放疗后的局部控制率和生存期优于淋巴结 >3cm者。食管癌术后锁骨上和纵隔淋巴结转移经放射治疗后局部可得到有效控制 ,多数病人死于远处转移     

Polymorphism of the HLA-DQA1 and -DQB1 genes of Han population in Jiangsu Province, China

The HLA genes, located on the short arm of human chromosome 6, encode peptides involved in host immune response, are important in tissue transplantation and are associated with a variety of infectious, autoimmune, and inflammatory diseases. Moreover, the HLA loci display an unprecedented degree of diversity and the distribution of HLA alleles and haplotypes among different populations is considerably variable. The expression of particular HLA alleles may be associated with the susceptibility or resistance to some diseases. In this study, the genetic polymorphism of HLA-DQA1 and -DQB1 in Jiangsu Han population was analyzed by polymerase chain reaction-sequence-based typing （PCR-SBT）.     

Electroacupuncture and Proliferative Knee Osteoarthritis： A Report of 50 Cases

针刺双侧内膝眼、犊鼻、阿是穴、阳陵泉、阴陵泉、足三里、梁丘、鹤顶和三阴交,内膝眼和阿是穴接电针,治疗了50例增生性膝关节炎患者.结果痊愈42例,好转6例,无效2例.     

Expression of ADAM8 and its clinical values in diagnosis and prognosis of hepatocellular carcinoma

ADAM8 behaves as an active metalloprotease in vitro, hydrolyzing myelin basic protein and a variety of peptide substrates based on the cleavage sites of membrane-bound cytokines, growth factors, and receptors. Other studies have demonstrated overexpression of some ADAM family proteins in a variety of human tumors, but no report is available on the actual expression of ADAM8 and the correlation between clinicopathologic features and prognosis of hepatocellular carcinoma (HCC) patients. In this study, serum levels of ADAM8 were measured by ELISA in 126 patients with HCC, 50 patients with liver cirrhosis (LC), and 50 healthy individuals. The expression of ADAM8 in liver tissue was further studied using Western blotting in 126 patients with HCC and 50 with LC. The correlations between ADAM8 status and various clinicopathological parameters including survival were analyzed. Survival analysis was performed using the Kaplan?CMeier method and Cox's proportional hazards model. The ELISA assay showed that the serum levels of ADAM8 in the HCC, LC, and healthy groups were 136.4?±?34.5, 64.2?±?20.1, and 63.2?±?22.7?U/ml, respectively. Analysis of variance was used for inter-group comparison, and differences were found between the HCC group and the other two groups (both P?<?0.001), while no difference was found between the LC group and the healthy group (P?=?0.365). Western blotting assay showed that ADAM8 protein expression was detected in 62.7?% (79/126) HCC and in 32?% (16/50) LC tissues. Further, ADAM8 expression was associated closely with serum AFP elevation, tumor size, histological differentiation, tumor recurrence, tumor metastasis, and tumor stage. Kaplan?CMeier survival analysis showed that patients with ADAM8-positive tumors had a shorter postoperative survival time than those with ADAM8-negative tumors (P?<?0.001). Multivariate analysis revealed that ADAM8 expression was an independent prognostic parameter for the overall survival rate of HCC patients. These findings provide evidence that the expression of ADAM8 serves as a poor prognostic biomarker for HCC. ADAM8 may be a potential target of antiangiogenic therapy for HCC.     

Complete remission of leiomyosarcoma with lung and brain metastasis by chemoradiotherapy after surgery

Nowadays, leiomyosarcoma is stil dif icult to early diagnosis, has no standard treatment to fol ow, and the thera-peutic value of surgery, chemotherapy and radiotherapy haven’t been evaluated ef ective...     

卡托普利对糖尿病血管功能损伤的抑制作用研究

目的:探讨卡托普利对糖尿病血管功能损伤的抑制作用。方法:本院2011年3月～2012年3月就诊的2型糖尿病患者100例,随即分为对照组和观察组。对照组患者服用阿卡波糖,观察组在阿卡波糖组治疗基础上加服卡托普利,疗程均为8周。测量治疗前后两组患者血糖、血脂、血管弹性参数。结果:联合使用卡托普利的患者血糖、血脂及血流变指标明显下降,动脉内膜中层厚度明显下降,内皮依赖性舒张反应升高。结论:卡托普利能够抑制糖尿病血管功能损。     

不同回灌堆体和回灌方式对纳滤浓缩液回灌效果的影响

分别以4 a的老龄垃圾和焚烧厂产生的灰渣为堆体,采用水平和垂直2种回灌方式,研究工程中最适合的回灌参数。结果表明:老龄垃圾堆体中的微生物降解进水中部分COD,使得出水COD有所下降。而炉渣堆体中对进水只有一定的物理截留作用。炉渣堆体对于NH4+有着吸附和离子交换的作用,导致初期出水的NH3-N较低。同时,炉渣堆体的回灌出水中电导率较高,影响后续产生的渗沥液的处理。在回灌方式方面,水平回灌虽然进水的停留时间较长,但是出水的COD和电导率没有太大的变化,NH3-N反而升高。