匹多莫德口服液对急性加重期慢性阻塞性肺病患者细胞免疫的影响

目的:探讨匹多莫德口服液对慢性阻塞性肺病(COPD)急性发作期患者细胞免疫功能的影响.方法:将COPD急性发作期患者60例分为治疗组和对照组各30例,另选30例体检的健康老年人为正常对照组;对照组给予祛痰、平喘、抗炎、给氧常规治疗,治疗组除常规治疗外给予匹多莫德口服液,每天2次,每次0.4g,疗程14 d,比较两组治疗...     

Effects of Matrine on JAK-STAT Signaling Transduction Pathways in Bleomycin-Induced Pulmonary Fibrosis

The current study aims to investigate the effects of matrine on the JAK-STAT signaling transduction pathways in bleomycin (BLM)-induced pulmonary fibrosis (PF) and to explore its action mechanism. A total of 72 male C57BL/6 mice were randomized into the control, model, and treatment groups. PF models were established by instilling BLM intratracheally. The treatment group was given daily matrine through gastric lavage. Six mice were sacrificed in each group at 3, 7, 14, and 28 days. The lung tissues were observed using hematoxylin-eosin staining. The expression of JAK, STAT1, and STAT3 was observed using immunohistochemistry and then determined using real-time polymerase chain reaction. Alveolitis and PF significantly improved in the treatment group compared with the model group (P < 0.05). The expression of JAK, STAT1, and STAT3 in the model group increased at day 7, peaked at day 14 and then decreased, but the expression was still higher than that in the control group at day 28 (P < 0.05). The three indices in the treatment group were significantly lower than those in the model group at any detection time point (P < 0.05). PF causes high expression of JAK, STAT1, and STAT3. Matrine exerts an anti-PF effect by inhibiting the JAK-STAT signaling transduction pathways.     

重组人血管内皮抑素联合顺铂体外干预人胃癌细胞生长的研究

目的研究重组人血管内皮抑制素（恩度）联合顺铂对人胃癌细胞株BGC823和SGC7901增殖及凋亡的影响,并探讨其抑制增殖及诱导凋亡的机制。方法采用四甲基偶氮唑盐（MTT）法检测不同浓度顺铂单药（单药组）及其与恩度联合应用（联合组）对BGC823和SGC7901细胞的增殖抑制率;采用流式细胞术分析凋亡细胞比例;采用Westernblot法分析增殖相关蛋白Ki-67及凋亡相关蛋白Bcl-2表达的变化。结果联合组对BGC823和SGC7901细胞的抑制率明显高于单药组;流式细胞术结果表明,正常对照组、单药组和联合组细胞的早期凋亡率依次增加;与单药组比较,联合组Ki-67、Bcl-2基因（蛋白）表达率显著降低。结论与顺铂单独应用相比,恩度联合顺铂对BGC823和SGC7901细胞增殖的抑制作用和诱导凋亡能力均明显增强。     

桂林西瓜霜喷雾剂治疗化疗后非外渗性静脉炎的疗效观察

[目的]探讨桂林西瓜霜喷雾剂对化疗后非外渗性静脉炎的治疗效果.[方法]将59例静脉炎病人随机分为两组,观察组30例,使用桂林西瓜霜喷雾剂加金霉素眼膏调和后敷于静脉炎处,对照组29例使用50％硫酸镁敷于静脉炎处;治疗3d后比较其治疗效果及不良反应.[结果]观察组总有效率为100.0％,对照组总有效率为86.2％,两组比较差异有统计学意义（P＜0.05）;两组均未见皮肤过敏.[结论]桂林西瓜霜喷雾剂与金霉素眼膏调和外敷可有效治疗化疗后非外渗性静脉炎.     

Bcr-Abl酪氨酸激酶抑制剂联合用药治疗慢性粒细胞白血病研究进展

以伊马替尼为代表的酪氨酸激酶抑制剂对慢性粒细胞白血病(CML)的治疗产生了重要影响,但其耐药性已成为CML治疗领域的关键问题。伊马替尼、尼洛替尼、达沙替尼以及普纳替尼等Bcr-Abl酪氨酸激酶抑制剂与其他药物联合使用,可以协同抑制Bcr-Abl及其磷酸化蛋白的表达,显著降低STAT5、CRKL、ERK5等信号通路的磷酸化水平,改善肿瘤微环境,降低肿瘤细胞多药耐药性,已在基础研究和临床Ⅰ期研究中取得阶段性成果,为治疗CML提供新策略。     

后腹腔镜肾脏切除术35例报告

目的探讨后腹腔镜肾脏切除术的手术方法及临床效果。方法2005年1月至2008年12月行后腹腔镜肾切除术35例,其中肾癌根治术11例,肾盂癌根治术1例,单纯肾切除术23例。站果35例均顺利完成腹腔镜手术。手术时间45-305min,平均（72.3±24.0）min,出血50-500mL,平均（102±56）mL,术后平均住院（7.2±3.1）d。术后随访3-51个月,患者均生存。结论腹腔镜肾切除术具有创伤小、术中出血少、术后恢复快等优点,是一种安全、有效的治疗方法。     

前列腺增生经尿道电切术近期出血的原因和处理

目的探讨经尿道前列腺电切术（TURP）治疗前列腺增生术后近期出血的原因与处理方法。方法对本院12年来前列腺增生经尿道电切术后近期出血的52例患者进行回顾分析其原因及止血方法。结果出血的原因主要有患者高龄、高危等和在治疗中处理失当等。41例经保守治疗而好转,11例经保守治疗无效,麻醉后重新置入电切镜,冲洗血凝块,充分电凝、止血后好转。结论全面的术前分析、准确的术中和术后处理,是减少前列腺增生患者TURP术后出血的关键。     

经椎间隙减压与椎体次全切除术治疗相邻节段颈椎病的比较

目的比较前路经椎间隙减压与椎体次全切除术治疗相邻节段颈椎病的临床疗效。方法回顾性分析2003年5月～2008年5月由同一组医师行颈前路手术治疗的相邻双节段颈椎病患者21例。依据手术方式分为2组。A组,椎间隙减压联合椎间融合器植骨融合组9例;B组,保留椎体后壁的颈前路椎体次全切除减压联合钛网植骨融合组12例。比较2组手术时间、术中出血量、住院花费、JOA评分、颈椎生理曲度及椎间高度变化,并观察植骨融合情况。结果B组中有2例原计划为行经椎间隙减压,术中改为行保留椎体后壁的次全切除减压术式。所有患者均随访18个月以上,平均22个月。B组手术时间略短于A组,出血量略低于A组,但差异均无统计学意义（P〉0．05）。末次随访时,2组的JOA评分差异无统计学意义（P〉0．05）。2组均无内置物松动或下沉。A组治疗费用高于B组,差异有统计学意义（P〈0．05）。2组术后3个月椎间高度及生理曲度较术前均有提高,但2组间差异无统计学意义（P〉0．05）。末次随访时植骨融合率为100％。结论2种方法治疗连续双节段颈椎病均可获得可靠临床疗效,并能获得术后稳定性。但椎间隙减压操作相对复杂,治疗费用略高。如术中出血较多影响术野,或者椎体后缘骨赘较多,难以操作,应及时更换手术方式。     

Polymorphisms in the oligoadenylate synthetase gene cluster and its association with clinical outcomes of dengue virus infection

Oligoadenylate synthetases (OAS) play an important role in the immune response against dengue virus. Single nucleotide polymorphisms (SNPs) in the OAS genes are known to affect OAS activity and are associated with outcome of viral infections. Polymorphisms in the OAS1 (rs1131454 and rs10774671), OAS3 (rs2285932 and rs2072136) and OAS2 (rs15895 and rs1732778) genes were studied using PCR followed by restriction fragment length polymorphism methods in 109 patients hospitalized for dengue (DEN) and 105 healthy controls (HCs) who have no documented evidence of symptomatic dengue. The two locus haplotype of OAS2 G-G was significantly higher in all patient groups [DEN vs. HCs, P = 0.0041, P corrected (Pc) = 0.012, Odds ratio (OR) 1.73 95% CI 1.16–2.59] while the four locus haplotype of OAS3-OAS2 C-G-A-G was significantly lower in all dengue patient groups [DEN vs. HCs, P = 0.0054, Pc = 0.0486, OR 0.09, 95% CI 0.00–0.64] compared to controls. When the six locus haplotypes involving OAS1, OAS3 and OAS2 polymorphisms were analyzed and compared, the frequency of the haplotype A-A-C-A-G-G was significantly higher [P = 0.0267, Pc = 0.486, OR 2.34, 95% CI 1.08–4.91] and the frequency of the haplotype A-A-C-G-G-A was significantly lower in DHF cases [P = 0.014, Pc = 0.252, OR 0.12, 95% CI 0.01–0.85] compared to healthy controls. The results suggest that OAS1-OAS3-OAS2 haplotypes are associated with differential susceptibility to clinical outcomes of dengue infection.     

腹腔镜辅助经阴道子宫切除术临床效果分析

目的 通过腹腔镜辅助经阴道子宫切除术(laparoscopically assisted vaginal hysterectomy,LAVH)与经腹全子宫切除术(trans-abdominal hysterectomy,TAH)的临床效果比较,分析LAVH的临床价值。方法 将宜兴市中医医院2010年10月至2011年8月行LAVH患者36例作为研究组,抽取同期行TAH患者36例作为对照组,将两组患者临床资料进行回顾性分析。结果 LAVH组患者术后肛门排气时间及术后住院时间均较TAH组短,术后疼痛程度及术后病率也较TAH组轻,两组比较差异有统计学意义(P<0.05);手术时间、术中出血量两组比较差异无统计学意义(P>0.05)。结论 LAVH具备腹腔镜手术及阴道手术两者之优点,具有损伤小、恢复快、切口小、疼痛轻等优点,值得临床推广应用。