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1.
EB病毒相关性疾病病理学研究的进展   总被引:17,自引:5,他引:12  
报告作者及其同事在鼻咽癌高发的广州地区所做EB病毒相关性疾病病理学研究的进展.内容包括:①EB病毒病理生物学;②EB病毒相关性疾病病理学;③检测组织中的EB病毒;④EB病毒血清学.重点是EB病毒相关性疾病的病理学以及评估各种检测EB病毒相关性疾病患者组织和血清中EB病毒的方法.  相似文献
2.
Background Intestinal T-cell lymphoma (ITCL) is a heterogeneous lymphoid neoplastic group with variable clinical and pathological features. ITCL in oriental countries is different from enteropathy-type intestinal T-cell lymphoma (ETCL) in relation to celiac disease and Epstein-Barr virus (EBV). The objective of this study was to investigate the clinicopathological features, immunophenotype, expression of cytotoxic molecule (TIA-1), T-cell receptor (TCR)-γ gene rearrangement, and Epstein-Barr virus (EBV) latent infection in primary ITCL without celiac disease in Chinese.Methods The clinical data of 42 patients were analyzed, and the patients were followed up. Compared with human reactive lymphoid tissues, in situ hybridization for EBER1/2, polymerase chain reaction for TCR-γ gene rearrangement, and immunohistochemical staining for immunophenotypes, TIA-1 and EBV latent membrane proteins (LMP-1) were investigated. Survival curves of different clinicopathological features, immuno-phenotypes, expression of LMP1, TCR-γ gene rearrangement and therapy were analyzed.Results Three fourths of the patients suffered from ITCL in China were men with a peak age incidence in the 4th decade. Common presenting features included fever and hemotochezia. The prognosis was poor with a median survival of 3.0 months. The lesions were mostly localized in the ileocecum and colon. About 38/42 (90.5%) patients demonstrated pleomorphic medium-sized on large cells. Histological features of celiac disease were rarely seen. All 42 patients with ITCL revealed CD45RO positive. Neoplastic cells partially expressed T-cell differentiated antigens (CD3ε, CD4, CD8) and NK cell associated antigen (CD56). The positive frequency of CD3ε, CD4, CD8 and CD56 was 28/42 (66.7%), 7/42 (16.7%), 10/42 (23.8%) and 12/42 (28.6%) respectively. Thirty-nine cells (92.9%) expressed TIA-1, but none expressed CD20 and CD68. More than half of the patients (64.3%, 64.3% and 59.5%) revealed TCR-γ gene rearrangement by three different TCR-γ primers respectively. EBER1/2 was detected in 41 (97.6%) of the 42 patients. The expression frequency of LMP-1 was 38.1% (16/42).Conclusions Primary ITCL without celiac disease in Chinese is a special highly EBV-associated clinicopathological entity. There are few similarities in patients with celiac disease in western countries. A small proportion of primary ITCLs in Chinese and extranodal NK/T-cell lymphoma of nasal type belong to the same spectrum.  相似文献
3.
Objective To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency of EBV DNA assay for serodiagnosis of NPC.Methods The plasma and serum were obtained from 58 AHAs and 66 untreated NPC patients. EBV DNA W-fragment was detected using nested ploymerase chain reaction (PCR). Immunoenzymatic assay for titration of IgA-VCA was also adopted. Results EBV DNA detection rate (84.85%) in the plasma/serum of 66 NPC patients was significantly higher than that (10.34%) in 58 AHAs. The sensitivity of plasma/serum EBV DNA assay (0.8485) was higher than that (0.8030) of titrating IgA-VCA (positive criterion≥1∶40) though the specificities of these two tests were the same (0.8966). The correct rate, predictive value of a positive test, and Odds ratio of dual positivity (0.8387, 0.9792 and 141.0, respectively) were higher than those of single positivity either to plasma/serum EBV assay (0.5242, 0.7333 and 1.1423, respectively) or to IgA-VCA≥1∶40 test (0.4839, 0.5385 and 1.0480, respectively). Conclusion The EBV DNA detection in the plasma/serum using nested PCR may be a useful indicator for serodiagnosis of NPC.  相似文献
4.
简单可行的EB病毒转化B淋巴细胞方法的探讨   总被引:8,自引:0,他引:8  
介绍两种简便可行的EB病毒转化B淋巴细胞的方法,即微量全血法和冻存全血法。与环孢霉素A法相比较,这两种方法具有操作简单,所需血样少,无需环孢霉素A等优点。这些特点尤其是在进行大量样本转化时显得更为突出。用微量全血法和冻存全血法共进行79例转化试验,转化成功率分别为46%与85%。对于影响这两种EB病毒转化结果的因素也进行了讨论。  相似文献
5.
Wang C  Ai M  Ren W  Xiao H  Li X  Tang F  Gu H  Yi W  Weng X  Deng X  Cao Y 《中华医学杂志(英文版)》2003,116(7):1022-1028
Objectives To identify whether Epstein-Barr virus (EBV) encoded latent membrane protein 1(LMP1) can induce tumor necrosis factor receptor-associated factor 1 (TRAF1) expression and promote its anti-apoptosis activity via the NF-KB signaling pathway, and assess that LMP1 suppresses apoptosis in nasopharyngeal carcinoma (NPC).Methods A stable transfected cell line HNE2-LMP1 was established by introducing LMP1 cDNA into HNE2 cells. Transactivation of TRAF1 was determined by luciferase reporter assay, while expression of TRAF1 mRNA was detected by RT-PCR and expression of TRAF1 protein and caspase 3 by Western blot analysis. Apoptosis activity was observed through fluorescence staining.Results LMP1 induced TRAF1 expression in NPC cells and caused a decrease in apoptosis. This induction could be blocked by antisense LMPI. Moreover, LMPl-mediated induction of a TRAF1 promoter-driven reporter gene was significantly impaired when the KB site KB1 or KB5 was disrupted,whereas mutation of κB3 had only a minor effect on LMP1 dependent up-regulation of the reporter gene.Conclusion LMP1 induces TRAF1 expression and promotes its anti-apoptosis activity via the NF-κB signaling pathway, which may be one of the mechanisms that LMP1 uses to suppress apoptosis in NPC cells.  相似文献
6.
目的从蛋白和DNA两个水平初步检测成都地区结外鼻型NK/T细胞淋巴瘤中LMP1的表达,并探讨与预后的关系。方法应用免疫组化和PCR技术检测67例结外鼻型NK/T细胞淋巴瘤LMPl的表达,并应用Kaplan—Meier曲线分别比较LMP1蛋白和LMP1DNA阳性表达组与阴性表达组的生存率。结果LMP1蛋白阳性表达10例(14.93%),LMP1DNA阳性表达56例(83.58%),LMP1总检出率83.58%。LMP1蛋白(P=0.678)和LMP1DNA(P=0.943)表达均与预后无明显关系。结论LMP1与成都地区结外鼻型NK/T细胞淋巴瘤关系密切;结外鼻型NK/T细胞淋巴瘤中LMP1蛋白水平和DNA水平表达不一致;LMP1的表达与预后无明显关系。  相似文献
7.
建立巴马长寿老人经EBV转染永生细胞株的方法   总被引:7,自引:0,他引:7  
目的:建立用EBV转化长寿人群外周血淋巴细胞永生细胞株(LCL)的方法。方法:用EBV份百岁以上健康老人,20份89-99岁健康老人及8份66岁以下健康人的血样共36份。采用20%FBS RPM11640培养液,配合环孢菌素A、植物血凝素(PHA),置37℃5%CO2培养箱内培养。结果:成功地获得24份永生细胞株(其中,百岁老人6份,89-99岁老人12份,66岁以下6份)。结论:采血后24-48h之间接种,转化成功率高。转染后2-3周,大部分标本出现细胞分裂、变形,标志着转化成功。超过5周仍无细胞分裂者即发生细胞凋亡。PHA有缓解细胞凋亡的作用,但不能使细胞分裂。污染是引起转染细胞株凋亡的主要因素。严格的无菌操作、保持培养细胞的适当密度、适时换液、打散较大的细胞克隆团是培养LCL的关键。  相似文献
8.
Objectives To investigate the prevalence of sinonasal lymphoepithelial carcinoma (SNLEC) in Guangzhou, a high incidence area of nasopharyngeal carcinoma (NPC), and to dete ct whether it is associated with Epstein-Barr virus (EBV) infection. Methods Twenty confirmed SNLEC specimens were collected from all of the sinonasal carcin oma biopsies performed in the 8 years 1989-1996 at the Sun Yat-sen University o f Medical Sciences. EBV encoded early RNAs were detected by use of in-situ hyb ridization. A variety of antigens, including the EBV nuclear antigen 1, latent membrane protein 1, BZLF1 protein, diffuse early antigen, viral capsid antigen a nd membrane antigen, were detected using immunohistochemistry. Additionally, 36 NPC specimens were used for comparison. Results Twenty SNLECs were identified. Seventeen SNLECs were developed in the nasal cav ity, and 3 in the maxillary sinus. The mean age (46.25 y), male to female ratio (3∶1), histopathology and lymphoinfiltration of the 20 SNLECs were id entical with those of the 36 NPCs. Thirteen (65.0%) of the 20 SNLECs showed an expansive growth pattern, while 27 (75.0%) of the 36 NPCs showed an infiltrat ing or mixed growth pattern. The majority of cancer cells in all of the 20 SNLE Cs showed EBV encoded early RNAs. The EBV nuclear antigen 1 expression of SNLEC was less intensive than that of NPC. The expression rate of latent membrane pr otein 1 for SNLEC (3/20,15%) was lower than that for NPC (19/36, 52.8%). The e xpression rates of BZLF1 protein (2/20, 10.0%), diffuse early antigen (19/20, 9 5.0%), viral capsid antigen (15/20, 75.0%), and membrane antigen (13/20, 65.0 %) for SNLEC were higher than those (0/36, 0.0%; 31/36, 86.0%; 18/36, 50%; and 14/36, 38.9%) for NPC. Conclusions SNLEC is not uncommon in Guangzhou. This tumour is also consistently associated with EBV infection like NPC. As compared to NPC, the EBV harbored in SNLECs s eems to express the EBV nuclear antigen 1 weakly and has a lower expression rate of latent membrane protein 1 as well as higher expression rates of EBV lytic pr oducts.  相似文献
9.
酶联免疫吸附检测血清EB病毒VCA-IgA和EA-IgG诊断鼻咽癌   总被引:6,自引:1,他引:5  
目的:评估采用酶联免疫吸附(ELISA)检测VCA-IgA和EA-IgD在鼻咽癌血清学诊断中的价值。方法:收集33例鼻咽癌患者和58例健康成年人的血清,用EISA检测VCA-IgA和EA-IgG,用免疫酶标法(IE)检测VCA-IgA和EA-IgA,并对两种检测方法的结果进行比较。结果:用ELISA和IE检测VCA-IgA的灵敏度均为0.9697。ELISA检测EA-IgG灵敏度(0.8788)高于IE检测EA-IgA(0.7879)。ELISA联合检测VCA-IgA和EA-IgG灵敏度(0.8485)高于IE联合检测VCA-IgA和EA-IgA(0.7879)。ELISA检测VCA-IgA和EA-IgG,33例鼻咽癌患者中,28例为双阳性,1例VCA-IgA阴性者EA-IgG阳性;4例EA-IgG阴性者VCA-IgA阳性。ELISA分别检测VCA-IgA和EA-IgG,9例无颈淋巴转移者与24例有颈淋巴结转移者两组均数之间均无统计学差异;19例临床早期患者(Ⅰ期和Ⅱ期)和14例晚期患者(Ⅲ期和Ⅳ期)两组均数之间亦均无统计学差异,结论:ELISA检测血清VCA-IgA和EA-IgG完全可以替代IE检测血清VCA-IgA和EA-IgA,获得更客观,有效的鼻咽癌血清学诊断,ELISA检测VCA-IgA和EA-IgG,这两个指标在鼻咽癌血清学诊断中具有互补作用,应联合应用,ELISA检测VCA-IgA和EA-IgG数值大小能反映鼻咽癌患者有无淋巴结转移及临床分期的早期。  相似文献
10.
黄芪抑制EB病毒壳抗原在体外细胞中表达的作用   总被引:6,自引:0,他引:6  
目的:探讨黄芪抑制EB病毒(EBV)壳抗原在体外细胞中表达的作用。方法:采用间接免疫酶法研究黄芪提取液对B95-8细胞壳抗原表达的抑制作用。结果:无毒性浓度的黄芪提取液对巴豆油。正丁酸联合激发的EB病毒壳抗原表达有明显抑制作用。抑制率随药物浓度增加而提高,结论:黄芪抑制EBV壳抗原表达效果好,有望在鼻咽癌预防方面起一定作用。  相似文献
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