急性阑尾炎全血细胞参数变化的临床意义

目的探讨急性阑尾炎全血细胞参数检测的临床意义。方法2006年我院收治的85例急性阑尾炎患者,术前行实验室检查对比术中所见(术后病理)进行分析,结果采用两样本等方差t检验。结果急性阑尾炎患者与正常对照相比,白细胞参数各指标及红细胞参数RBC、HGB和HCT均有非常显著性差异(P<0.01)。另外,急性阑尾炎患者中男性与女性相比,除白细胞参数中的LYM%和红细胞参数RBC、HGB及HCT存在非常显著性差异(P<0.01)外,其它指标无显著性差异。结论急性阑尾炎发作时,白细胞及红细胞参数的变化有助于急性阑尾炎的诊断。     

内毒素导致白细胞急剧升、降10倍一例报导

目的探索白细胞(WBC)数小时内急剧升、降原因。方法收集患者入院时首次血、尿细菌培养结果;取患者1周内7次EDTA-K2抗凝血标本,在STKS全自动血细胞分析仪上,与高、中、低定值全血质控品同时进行比对测定,每个样本平行分析4次求均值,同时检测患者6次血清标本内毒素含量,统计数据。结果测得仪器批内误差CV0.18%;其中14小时内5个样本WBC均数(x)分别为20.1×109/L、2.3×109/L、3.2×109/L、22.8×109/L、15.7×109/L;血、尿均分离到大肠埃希氏菌,内毒素试验阳性。结论内毒素导致WBC贴壁,造成病危期4小时内周围血液WBC急剧下降10倍;临床在分析实验结果时既要考虑内毒素导致WBC上升,又要考虑内毒素致使WBC贴壁造成急剧下降现象。     

Rapid accurate absolute granulocyte count determination during severe leukopenia using the flow cytometer

The absolute granulocyte count (AGC) in 125 blood samples from patients with total white blood cell counts of less than 1,000/microliter was estimated using three different methods, which were then compared for efficiency and accuracy. The three methods were 25 cell differential counts using Wright's-stained blood smears, granulocyte percentage estimates from WBC counting chambers, and combined narrow- and wide-angle light-scatter characteristics determined on a flow cytometer. A survey of clinical laboratories at University Hospital Cancer Centers revealed that the smear differential was the most-often-used method in those laboratories even when less than 25 cells could be counted. Consequently the data obtained from the counting chamber and flow cytometer methods were compared to the smear differential "standard" using linear regression, and outliers were identified. There was good correlation between AGC determined by smear differential and WBC counting chamber (correlation coefficient .911) and excellent correlation between the AGC determined by smear differential and the flow cytometer method (correlation coefficient .970). The flow cytometer method used in this investigation required minimal specimen preparation, and test results were available at a rate of 60 seconds/sample. The ease of sample preparation, speed, and statistical reliability of test results makes the flow cytometer an attractive alternate method of determining granulocyte counts on leukopenic patients as compared to the stained blood smear differential.     

Value of preoperative urine white blood cell and nitrite in predicting postoperative infection following percutaneous nephrolithotomy: a meta-analysis

BackgroundTo evaluate to what degree preoperative urine white blood cell (WBC) and urine nitrite (NIT) values are predictive of postoperative infections following percutaneous nephrolithotomy (PCNL).MethodsA systematic literature search was performed of the PubMed, Embase, Cochrane Library, Wanfang Data, National Knowledge Infrastructure (CNKI), and China Science and Technology Journal Database (CSTJ or VIP) online databases to identify relevant studies that examined the predictive value of urine WBC or NIT as risk factors for post-PCNL infection, and the search was finished on February 28, 2020. Two independent reviewers screened the relevant studies, extracted necessary data from the eligible case-control studies (CCS), and assessed the quality of included studies through the Newcastle-Ottawa scale (NOS). RevMan 5.3 software and the Stata 16.0 software were used to complete the statistical analysis of data. Results are expressed as odds ratio (OR) with 95% confidence intervals (CIs).ResultsAccording to the statistical analysis of 12 eligible studies involving 6113 patients, positive urine WBC (WBC+: OR =3.86, 95% CI: 3.03–4.91, P<0.001) and positive NIT (NIT⁺: OR =7.81, 95% CI: 5.44–11.21, P<0.001) in preoperative tests were identified as independent risk factors for postoperative infections following PCNL.ConclusionsIn summary, as risk factors for postoperative infections, the presence of preoperative urine WBC+ and NIT+ should be evaluated as part of clinical procedure, in order to reduce infections of PCNL.     

Effect of DW2282 on the induction of methemoglobinemia, hypoglycemia or WBC count and hematological changes

DW2282,(S)-(+)-4-phenyl-1-[1-(4-aminobenzoyl)-indoline-5-sulfonyl] -4,5-dihydro-2-imidazolone hydrochloride, is a new anticancer agent which is thought to exhibit a characteristic mechanism of action in the inhibition of tumor growth. In this study, we estimated the toxicities of DW2282 in mice. When mice were orally dosed for five consecutive days at the dosages of 50, 100 and 150 mg/kg, DW2282 did not induce methemoglobinemia and hypoglycemia at any of these doses. However, increased ALT and AST values were observed in the 150 mg/kg dosing group, and white blood cells (WBC) were significantly decreased at all doses. However, the changes in WBC count, ALT and AST immediately reversed after the cessation of drug administration. In addition, we found that DW2282 did not cause an increase in hemolysis in human blood. Taken together, these data suggested that DW2282 may have a relatively low level of toxicity, and that there may be a quick recovery from any toxicity it does produce.     

机检和镜检尿中的WBC、RBC的结果不符分析

目的提高尿中的WBC、RBC检测的准确度.方法用尿液自动分析仪和显微镜分别检测尿的WBC、RBC的结果,并对检测结果不符原因进行分析.结果对于WBC,在1 90例尿液中(机检为阴性),镜检出10例阳性(占5.3%),在80例尿液中(机检为阳性),镜检出9例阴性(占11.3%);对于RBC,在200例尿液中(机检为阴性),镜检出2例阳性(占1.0%),在100例尿液中(机检为阳性),镜检出23例阴性(23.0%).结论必要时,对尿液应同时进行机检和镜检,这样才能够相互弥补,减少误差,提高尿中的WBC、RBC检测的准确度.     

67例单采血小板纯化效果分析

目的 :对因各种因素造成的白细胞和红细胞含量较高的单采血小板进行纯化分析。方法 :把67例此类单采血小板随机分为两组。35例采用转移离心法纯化 ,32例采用倒置离心法纯化。对纯化前后及两种方法之间的纯化效果进行比较分析。结果 :经两种方法纯化后 ,血小板损耗均不明显 (P>0 05) ,白细胞和红细胞去除差异有显著性 (P<0 001) ,两方法间的纯化效果略有差异 (P<0 005)。结论 :经两种方法纯化后 ,均能去除混杂在单采血小板中的大部分白细胞和红细胞 ,转移离心法的纯化效果优于倒置离心法 ,纯化时可根据实际情况进行选择。     

C-反应蛋白在妇科疾病中的临床应用

目的:探讨血中C-反应蛋白(CRP)的检测对妇科炎症疾病与非炎症疾病的鉴别诊断和疗效判断价值。方法:检测125例患者血中CRP含量及白细胞计数(WBC)。结果:通过对125例妇科患者血中CRP检测,表明炎症性疾病与非炎症性疾病CRP含量有显著性差异。盆腔炎患者中CRP的敏感性明显高于白细胞计数的敏感性;盆腔炎患者治疗前后CRP和WBC的比较,CRP变化较之WBC变化更符合临床治疗效果。结论:血CRP的检测有助于妇科炎症性疾病与非炎症性疾病的鉴别诊断,动态检测其变化对疗效判断有实用价值。     

白介素-18等炎性标记物与冠状动脉病变程度的关系研究

目的：探讨炎性标记物白介素-18和白细胞计数在不同程度的冠状动脉病变中的表达情况，以期根据炎性标记物的血浆水平甄别冠状动脉病变的严重程度。方法：测定经冠脉造影确诊的急性冠脉综合征（ACS）病人血浆中白介素-18（IL-18）的含量以及全血白细胞计数，然后比较不同程度冠脉病变组的炎性标记物水平；并比较糖尿病（DM）组和非DM组的差异。结果：白介素-18的含量以及全血白细胞计数在冠状动脉复杂病变组明显高于简单病变组（P〈0．05）。合并有DM的ACS病人白介素-18的含量以及全血中的白细胞计数水平明显高于非DM组（P〈0．05）。结论：在不同程度的冠状动脉病变组和不同危险因素组炎性标记物水平存在着显著性差异。     

养正消积胶囊对CaEs-17细胞成瘤组织及对荷瘤鼠生存状态与白细胞分类的影响

目的：探讨养正消积胶囊对荷瘤裸鼠食管鳞癌的抑制作用及对其生存状态、白细胞分类的影响。方法：食管麟癌CaEs-17细胞株经培养并接种于裸鼠皮下，随机分组后给予3种不同剂量的养正消积胶囊灌胃，并与顺铂对照。观察荷瘤裸鼠生存状况，测量肿瘤抑制率并检测裸鼠白细胞分类。结果：养正消积胶囊对荷瘤裸鼠食管癌组织具有抑制作用，对其日常生活质量，白细胞、淋巴细胞数量具有维持作用，与顺铂相对照差异显著（p＜0.05）。结论：养正消积胶囊能抑制荷瘤裸鼠食管癌组织的增殖，且有利于改善其生存状态与白细胞水平。