间断纵行缝扎硬化注射治疗直肠黏膜内脱垂68例临床分析

目的:观察和评价间断纵行缝扎硬化注射治疗直肠黏膜内脱垂的临床疗效。方法:将68例直肠黏膜内脱垂患者随机分成两组,治疗组(36例)采用经间断纵行缝扎硬化注射治疗,对照组(32例)采用单纯硬化注射治疗。并对临床资料进行分析总结。结果:术后3个月随访,治疗组与对照组的症状、体征总得分比较,治疗组均优于对照组。结论:纵行缝扎硬化注射治疗后症状消除快,近远期疗效佳。     

起搏器高度依赖性患者起搏器置换方法与疗效探讨

目的 :探讨起搏器高度依赖性患者起搏器置换方法与疗效。　方法 :对 8例起搏器高度依赖性患者 ,在起搏器与电极的接口松解前 ,应用在体起搏器间断接触法 ,使自身逸搏心律得已恢复 ,然后再行松解接口以及必要的起搏参数测试。　结果 :起搏器离体次数为 3～ 7(5± 2 )次 ,最长逸搏间期为 3.38～ 6 .2 0 (4.5 1± 1.11)s,自身逸搏心率为 30～ 4 3(38± 5 )次 /min ,均顺利完成起搏器置换。　结论 :在体起搏器间断接触法切实可行 ,能够明显提高起搏器高度依赖性患者起搏器置换的安全性     

Amplified fragment length polymorphism analysis of the VNTR locus D1S80 in central Spain

Summary The polymorphism of the D1S80 locus has been analyzed in a population sample of 203 unrelated individuals living in Madrid (central Spain) by PCR and subsequent semi-dry discontinuous polyacrylamide gel electrophoresis (Tris-chloride/Tris-glycine buffer system) followed by silver staining, The electrophoretic system described in this study offers high resolution in the separation of the different D1S80 alleles allowing the detection of microvariability around the allele T22 in the spanish population. Twenty different alleles containing 17–40 repeats of the basic 16bp unit were distinguished. The alleles T18 and T24 were found to be relatively common in Spain, as in other populations:, with frequencies of 0.224 and 0.372, respectively. No evidence of significant deviations from Hardy-Weinberg equilibrium was found in these preliminary population data.     

Separation of tumor-infiltrating lymphocytes from tumor cells in human solid tumors A comparison between velocity sedimentation and discontinuous density gradients

The separation of viable tumor-infiltrating lymphocytes (TIL) from surgical biopsies of human solid tumors was achieved by velocity sedimentation at unit gravity or by discontinuous density gradients. The two methods were adapted to small volumes and cell numbers not exceeding 1 × 10⁸. The recovery, purity and composition of the TIL-enriched fractions were comparable in the two methods. Density gradients were more rapid, simpler and more practical for preparation under sterile conditions of TIL from clinical material than velocity sedimentation. Lymphocytes in the TIL-enriched fractions obtained by either of the methods were poorly responsive to mitogens. This poor responsiveness is a characteristic of the human TIL and seems to be related to effects exerted by tumor cells.     

Effect of resistive discontinuities on waveshape and velocity in a single cardiac fibre

A computer model of a one-dimensional cardiac fibre of resistively coupled cells is used to investigate the influence of the junction resistance on the nature of conduction. The results of the simulations are presented and indicate that the effect of the junction on both intracellular and extracellular waveshape and on the velocity of propagation depends on the size and frequency of the coupling resistance and the kinetics of the active membrane. Significant changes in these factors are not observed without the generation of prepotentials in the action potential upstroke. The absence of this ‘signature’ in microelectrode recordings of activity in ventricular muscle suggests that under normal conditions cardiac tissue behaves as a functional syncytium.     

定时与自主湿化气道对肺叶切除术后患者排痰效果的疗效分析

目的探讨定时与自主湿化气道对肺叶切除术后患者排痰效果的影响。方法选择肺叶切除术后患者97例，随机分成实验组49例、对照组48例。实验组患者在术后采用定时予自主湿化气道方法促进痰液排出；对照组患者采用常规方法湿化气道促进痰液排出。观察两组患者术后痰液黏稠程度显著低于对照组，咳痰能力和术后并发症的发生率。结果实验组与对照组比较，实验组术后12h、18h、24h、30h痰液黏稠度、咳痰能力优于对照组，差异有显著意义（P〈0．005）；术后并发症的发生率实验组低于对照组，差异有显著意义（P〈0．05）。结论肺叶切除术后采用定时与自主湿化气道法促进患者排痰效果显著优于常规湿化气道法，可广泛应用于临床。     

精密输液器的使用对高压氧治疗中气管切开插管患者气道湿化的影响

目的：探讨精密输液器的使用对高压氧治疗中气管切开插管患者气道湿化的影响。方法：76例气管切开术后患者均采用精密输液器进行气道湿化，观察其治疗前和治疗第1、3、5、10天的刺激性咳嗽、痰阻次数、吸痰量、吸痰次数等指标。结果：至治疗后10天，患者的刺激性咳嗽、痰阻次数、吸痰量、吸痰次数等指标均显著改善（P〈0.01），痰液粘稠度亦显著降低（P〈0.05）。结论：使用精密输液器能达到理想的湿化效果，降低了患者的高气道反应，减少了对气道的刺激，并且操作简单、安全可靠，值得临床推广应用。     

需求满足式护理对提高胸外科机械通气患者微量泵持续气道湿化效果及舒适度的研究

目的研究需求满足式护理对提高胸外科机械通气患者微量泵持续气道湿化效果及舒适度的价值。方法将纳入本院胸外科机械通气使用微量泵持续气道湿化210例患者作为研究对象,按就诊顺序分为观察组和对照组各105例,对照组患者实施唱歌机械通气护理,观察组患者增加了需求满足式护理。对比2组患者的气道湿化情况、舒适度以及满意度情况。结果观察组患者的并发症发生率、日吸痰次数与日湿化液滴入量分别为6.67%、(2.68±1.27)次/d、(185.73±56.89)mL/d,均优于对照组的17.14%、(5.41±2.17)次/d、(113.56±43.75)mL/d,差异有统计学意义(P<0.05);观察组患者的舒适度与满意度评分分别为(8.73±0.25)分、(8.86±0.37)分,均优于对照组的(8.42±0.27)分、(8.45±0.34)分,差异有统计学意义(P<0.05)。结论使用需求满足式护理可优化胸外科机械通气患者微量泵持续气道湿化的舒适度与湿化效果。     

非连续密度梯度离心法分离红细胞的影响因素分析

目的 分析非连续密度梯度分离法分离红细胞效果的影响因素,探讨各因素对分离结果的影响程度.方法 采用正交设计比较不同分离条件(离心力、离心时间、离心温度)的分离效果,应用多元线性回归统计方法分析不同个体参数(红细胞平均体积、细胞浓度、个体年龄)对分离效果的影响.结果 离心力、离心时间、标本红细胞平均体积、红细胞浓度、个体年龄都对分离结果有影响,其中红细胞平均体积起主导作用.结论 不同分离条件以及同一分离条件下不同标本,其分离效果均有差异,通过对影响因素的人为控制,可以得到相对稳定的分离效果.