法尼酯X受体激动剂GW4064对人胆管癌细胞株QBC939生长的影响

目的:研究法尼酯X受体(FXR)激动剂GW4064对人胆管癌细胞株QBC939增殖和凋亡的影响,并检测QBC939细胞株中FXR基因表达的改变。方法:用浓度为0.5、1.0和5.0μmol/L的法尼酯X受体(FXR)激动剂GW4064刺激人胆管癌细胞株QBC939,用四甲基偶氮唑蓝(MTT)比色法检测24、48 h后细胞活力的变化,以流式细胞仪技术检测药物刺激48 h后肿瘤细胞凋亡的变化,并以RT-PCR检测QBC939细胞株中FXR mRNA表达的改变。结果:浓度为1.0、5.0μmol/L的GW4064在作用24 h后即能显著抑制胆管癌细胞生长(P0.05);在药物作用48 h后,所有浓度的GW4064均能对肿瘤细胞生长起明显的抑制作用(P0.05)。流式细胞仪检测细胞凋亡的结果显示,药物刺激48 h后,对照组凋亡细胞占细胞总数的3.38%,加入0.5μmol/L浓度GW4064的细胞凋亡率为10.02%;而加入1.0和5.0μmol/L浓度的GW4064后,细胞凋亡率明显升高至23.74%和42.11%。RT-PCR结果显示,所有浓度的GW4064于刺激48 h后,QBC939细胞株中FXR基因表达增加,与对照组相比均有统计学差异(P0.05)。结论:GW4064能通过特异性激动FXR有效抑制胆管癌细胞株QBC939的细胞活力,并促进其凋亡,这一过程可能与其上调FXR的表达有关。     

内脏脂肪组织FOXC2mRNA表达水平与2型糖尿病的关系

背景:FOXC2在能量代谢等方面发挥重要作用,与脂肪细胞分化密切相关,且FOXC2基因在抵御2型糖尿病及代谢综合征相关因素方面有重要作用.目的:检测云南地区人群大网膜脂肪组织中FOXC2 mRNA表达水平,探讨其与2型糖尿病的关系.方法:随机抽取在昆明医学院附属医院行腹部外科手术的2型糖尿病患者及非2型糖尿病患者各50例,RT-PCR检测其大网膜脂肪组织中FOXC2 mRNA的表达水平,并分析两组受试者的临床资料与FOXC2 mRNA水平的相关性.结果与结论:2型糖尿病患者腹部大网膜脂肪组织FOXC2 mRNA表达水平下降;FOXC2 mRNA表达水平在2型糖尿病组与空腹血糖、血压呈负相关,在非2型糖尿病组与空腹血糖呈负相关(P < 0.01).提示FOXC2与2型糖尿病的关系主要在于可以减轻胰岛素抵抗,改善胰岛素对糖代谢的调节,此外它的表达下降在糖尿病患者血压升高中起到一定的协同作用.     

rAAV—IGF—I诱导大鼠下颌髁突生长发育的实验研究

目的评价局部注射重组腺相关病毒介导胰岛素样生长因子-I（rAAV—IGF-I）对大鼠下颌髁突生长发育的影响。方法选用5周龄Sprague-Dawley（SD）大鼠20只，随机分为4组。在每只大鼠的左侧颞下颌关节腔内注射rAAV-IGF-I，右侧关节腔内注射生理盐水。手术后2周、4周、8周及12周后分别处死并取出下颌骨，拍摄两侧下颌骨的数码照片进行测量比较。结果实验组下颌髁突长度、髁突头宽度、髁突头中点及髁突头下点到下颌平面的距离从实验4周时出现明显差异。实验组下颌骨的长度及髁突前点到下颌平面的距离在实验8周及12周中明显长于对照组。4周及8周时实验组髁突长轴与下颌平面的夹角均大于对照组。结论局部注射rAAV—IGF-I能够促进大鼠下颌骨长度及高度的增加，为治疗下颌发育不足提供了新的方法。     

循环miR-189对系统性红斑狼疮的诊断意义和价值

目的:探讨血浆中miR-189在系统性红斑狼疮(system lupus erythematosus,SLE)组与正常对照组的表达量是否存在差异,以及循环miR-189对SLE潜在的诊断价值.方法:收集SLE患者为疾病组(n=40),设立正常对照组(n=23).抽提标本总RNA,并逆转录为cDNA,采用TaqMan探针法进行Rea-time PCR检测,观察miR-189的表达变化.结果:通过比较分析SLE组miR-189的表达量高于正常组,有统计学意义(P=0.001).对miR-189诊断特异性和敏感性分析得出受试者工作特征曲线(receiver operating characteristic curve,ROC)下面积AUC=0.798±0.055,有一定的诊断价值,同时将miR-189表达量与SLEDAI评分、抗ds-DNA和激素使用剂量作相关性分析,都无明显相关.结论:血浆游离miR-189可以作为一个SLE的潜在诊断标志物,有助于SLE的早期诊断,使患者得到早期的治疗.     

羊水过少84例临床分析

目的：研究分析经输卵管疏通术后再阻塞采取相应措施预防的效果.方法：对我院所收治的28例患者（A组）,经输卵管疏通术过程中加用透明质酸钠,在其术后行输卵管通液术与经盆腔炎治疗仪进行热疗治疗来预防术后的再阻塞与再粘连.另选择28例患者（B组）均单纯行手术治疗.结果：对于经输卵管疏通术中加用透明质酸钠,术后行输卵管通液术与盆腔炎治疗仪热疗的A组患者,同单纯行手术治疗的B组患者相比,A组要比B组的术后妊娠率高,且术后发生再阻塞率相对较低（P〈0.05）.结论：经输卵管疏通术后再阻塞采取输卵管通液术与经盆腔炎治疗仪热疗的效果良好,值得临床予以推广使用.     

请让小草顺其自然生长

5岁的女儿上学前班。有一天，我们一家三口正在吃晚饭，她对我说有个男同学老是亲她的脸蛋，我和她爸爸都不禁笑了。现在电影电视这么发达，什么超前的信息都会灌输给儿童。     

糖皮质激素治疗婴幼儿血管瘤

血管瘤是儿科临床常见病、多发病，具有很高的发病率，其中约50％在出生时即存在，其余多在生后2～4周出现，1岁内婴儿血管瘤的发病率可高达10％～12％。婴儿期血管瘤在生后数月至数年内有自行消退的可能，因此，等待血管瘤自行消退曾一度作为临床医生的首选治疗方法。但是，血管瘤自行消退的速度与生长速度相比是一缓慢的过程，仍然有相当数量的血管瘤生长在头面部，尤其是累及眼、耳、鼻、口咽等器官以及身体其它部位面积大、生长快的病例，影响美容和器官功能以及其它一系列并发症。应给予积极干预，以控制肿瘤生长、促进消退。     

Experimental Study on Stress Relaxation and Creep Properties of Human Thoracolumbar Vertebral Bodies and Intervertebral Discs

Objective：Underwater shock can produce extremely high accelerations, resulting in severe human injuries on shipboard, and human thoraco lumbar spines are prone to suffer from injuries by ship shock motion. To observe the viscoelasticity of thoracolumbar of young fresh cadavers, and to provide biomechanical parameters for both research and clinical practice. Materials and Methods：5 fresh young male cadavers （aged 22 to 31 years） were provided, and 15 thoracolumbar spinal anatomies of 5 samples were harvested within 1 hour of death. WE-10A universal testing machine was used for creep and relaxation tests.Results：Stress relaxation and creep deformation equations are derived from the biomechanics model and the measured and simulated curves are compared. The creep in vertebral bodies and intervertebral discs exhibited significantly changes in the first 5 min and 10 min, respectively. The stress rapidly decreased in the first 2 min, and then gradually went balance during the relaxation process. Conclusion：The change in creep rate is significant at early stage,and gradually slows down.This indicates that the differences between internal pressure and local pressure are decreased until balance. The simulated curve derived from equation coincides with the experimental data to a large degree, which states that the equation is rational and reliable.     

胎儿生长受限仔鼠脂肪组织中葡萄糖转运蛋白4的表达和细胞转位变化

Objective To investigate the effects of fetal growth restriction (FGR) on the expressions and translocation of glucose transporter 4 (GLUT4) in adipose tissue of male offsprings and its relationship with insulin resistance in FGR. Methods Male 8-week-old offspring from maternal with protein-malnutrition or normal diet were studied. The weight of rats at 8 weeks of age were obtained and insulin resistance index (IRI) was examined at 4 weeks and 8 weeks of age. Perinephric fat pads were harvested to measure the expression of GLUT4 mRNA by fluorescent quantitative RT-PCR and the GLUT4 protein level and insulin-stimulated transloeation were assessed by Western blot. Results Birth weight of FGR animals were significantly lower than that of control (t=6.399, P<0.01), but surpassed the control at 8 weeks with increased fasting IRI(P<0.05). The GLUT4 mRNA expression in adipose tissue of FGR rats (0.36± 0.04) was lower than that of control (1.01±0.11) (t=2.854, P<0.05). Declined total GLUT4 protein concentration(913.47±87.32) was also noted in the FGR group compared with the controls (1248.30± 131.52) (t=2.617, P<0.05). After administration of insulin, plasma membrane associated-GLUT4 concentration significantly increased to 897.03±102.87, which was equal to 2.12 times of the basal level (423.05±41.26) in control rats(t=4.759, P<0.01). However, insulin-responsive GLUT4 transloeation was markedly blunted in FGR rats(525.12±54.96) compared with controls, only increased to 1.38 times of the basal level (379.57±38.71)(t=2.083, P<0.05). Conclusions Decreased GLUT4 expression and insulin-stimulated translocation in adipose tissue of the male FGR offspring might be caused by exposure to protein malnutrition during pregnancy, which may disturb the uptake and utilization of glucose and subsequently be related to diabetes mellitus in later life.     

OGTT 1小时切点血糖在筛查糖尿病及糖调节受损中的临床意义

目的 探讨口服葡萄糖耐量试验(OGTT)负荷后1小时血糖(1hPG)筛查糖调节受损(IGR)和糖尿病(DM)的切点值及其与β细胞功能、胰岛素敏感性的关系.方法 4731名受试者行OGTT试验,应用接收者工作特征(ROC)曲线分析1hPG的切点值,按此切点分组后进行各组HOMA-IR、HOMA-β、胰岛素生成指数、形态指数的比较及相关性分析.结果 由ROC曲线得出筛查DM最佳的1hPG切点值为12.935mmol/L,灵敏度为81.52%,特异度为86.94%;筛查IGR最佳的1hPG切点值为9.815mmol/L,灵敏度为74.96%,特异度为77.86%.将整个人群按1hPG分为三组,各组间的胰岛素生成指数、形态指数、HOMA-IR、HOMA-β均有统计学差异(P均<0.01).且1hPG与胰岛素生成指数和HOMA-β负相关,与形态指数、HOMA-IR正相关(P均<0.01).结论 筛查DM的1hPG切点值为12.935mmol/L,筛查IGR的1hPG切点值为9.815mmol/L,且1hPG可反映胰岛β细胞功能和胰岛素抵抗程度.