兔颞下颌关节盘前移位的超微结构研究

我们的实验着重探讨经实验诱导兔颞下颌关节盘前移位后 ,关节盘及盘后区超微结构的变化。材料和方法 :①用 12只正常新西兰大白兔 ,体重 2 5～3 0ｋｇ ,手术组 10只 ,对照组 2只。②实验方法 :手术组动物经全身麻醉后 ,切开左侧关节 ,剪断关节盘的外侧及前附着 ,将关节盘向前牵引 ,缝合固定于颧弓上。右侧关节盘暴露后不作牵引缝合固定 ,为手术对照组。③标本的制作和观察 :取正常对照组 2只动物 ,手术组麻醉下取术后 2 4ｈ ,1、2、3、4周各 2只动物同一部位的关节盘和关节盘后区组织 ,制成透射电镜标本观察。结果 :手术组 :①术后 2 4ｈ :…     

新型生物医学材料——类金刚石膜的研究进展

类金刚石因具有良好的细胞相容性、血液相容性及高耐磨性高硬度等特点 ,而成为一种很有发展前景的生物膜材料。本文综述了 2 0世纪 90年代以来类金刚石的理化性质、生物学方面的研究进展 ,并对未来的发展进行了展望。     

32P-磷酸铬-聚 L-乳酸缓释粒子的制备及其放射性释出和生物分布

Objective The aim of this study was to prepare the 32P-chromic phosphate-poly(L-lac-tide) (32P-CP-PLLA) particles with different ratio of the materials and further examine their performance in-dex in vivo and in vitro and their intracorporeal distribution. Methods The erosion, degrading rates, de-layed release velocity and radioactivity self-absorption coefficient (RSAC) of 32P-CP-PLLA particles made from different materials were investigated and compared. After the implantation of 32P-CP-PLLA particles and the injection of 32P-CP colloids in the muscular tissues, the weight loss rate and the radioactivity release rate (RRR) of the particles were calculated. The intracorporeal distribution, radioactive half-life and bio-logical effect of 32P in the targeting sites were further studied. Statistical analysis was performed with SPSS 12.0, and one-way analysis of variance and t-test were used. Results 32P-CP-PLLA particles were of green cylinder, with regular shape and radionuclide distribution. The RSAC of the particles was of little re-lation with molecular weight of PLLA and proportional to the ratio of PLLA to CP. The extracorporeal release rate increased with the reduction of molecular weight of PLLA and with the increase of the ratio of PLLA to CP. The RRR reached peak when PLLA was 3 times of CP. The 32P-CP, released with the degradation and corrosion of the particle distributed mainly in the surrounding muscles of the particle. And the peak of per-centage activity of injection dose per gram of tissue (% ID/g) in liver, spleen and bone were 1. 7887, 1. 6401 and 1. 9470 respectively, much lower than that in the 32P-CP group (4.7523, 3.9712 and 4.3174 ; all t > 2.7, all P < 0.05). The % ID/g in other organs was much less. The radioactivity effective half-life in the targeting sites increased to about 13 d. There was widespread necrosis around the particles with no ex-istence of normal tissues among them. And no abnormality in spleen and liver was found. Conclusion As a better dosage form of pure β-particle emitter, 32P-CP-PLLA, which can increase the targeting radioactive dosage and effective half-life in the implanting sites, can be served as an potential implanting agent for onco-therapy with a better perspective.     

32P胶体局部给药防治H22移植瘤淋巴道转移的实验研究

目的探讨不同剂量的^32P胶体局部注射对小鼠H22移植瘤和区域淋巴结转移灶的治疗作用。方法应用小鼠H22腹水型肝癌淋巴道转移模型，通过肿瘤组织局部给药，观察^32P胶体在模型小鼠移植瘤、区域淋巴结及全身各器官、组织内的分布。结果^32P胶体局部给药后主要聚集在瘤体注射局部和区域淋巴结内，而在肝、脾、肺等脏器分布的活度较低。区域淋巴结聚集的活度随给药剂量增高而递增。治疗早期瘤体和胭窝淋巴结转移灶呈现局灶性坏死。后期移植瘤和区域淋巴结转移灶的瘤组织呈现出血、坏死。结论^32P胶体瘤体给药可在局部富集，并可经淋巴道转运、聚集于区域淋巴结，对肿瘤组织和邻近的淋巴结转移灶具有明显的杀伤作用。     

四季抗病毒合剂联合吴茱萸穴位贴敷治疗小儿疱疹性咽峡炎60例

目的:观察中成药四季抗病毒合剂口服联合中药吴茱萸涌泉穴贴敷治疗小儿疱疹性咽峡炎的临床疗效。方法:应用四季抗病毒合剂口服,中药吴茱萸穴位贴敷双侧涌泉穴治疗小儿疱疹性咽峡炎与单纯西医静脉输液治疗疗效进行对比。结果:四季抗病毒合剂联合穴贴敷治疗在减轻症状、缩短病程等方面与纯西药治疗无明显疗效差异,并且患儿易于接受,无明显不良反应。结论:本方法治疗本病有疏风清热解毒,通腑泻火的功效,疗效优于单纯西药治疗。     

胃癌单克隆抗体MG_7及CEA在人胃癌及癌前期病变的表达及临床意义

目的检测胃癌及癌前期病变胃癌单克隆抗体ＭＧ７、癌胚抗原（ＣＥＡ）的表达，以寻找胃癌早期诊断的有效途径。方法采用免疫组化ＡＢＣ法测定１２例正常胃粘膜标本、３２例癌前病变及４２例胃腺癌的ＭＧ７和ＣＥＡ的表达。结果正常胃粘膜无ＭＧ７和ＣＥＡ的表达。结论ＭＧ７和ＣＥＡ和异常表达可作为胃癌发生过程中生物学行为的早期指标。     

乳腺、胃和大肠黏液性腺癌中Ⅳ型胶原蛋白表达的研究

目的：研究乳腺、胃和大肠黏液性腺癌中Ⅳ型胶原蛋白的表达情况。方法：采用SP免疫组织化学法对120例石蜡标本进行检测：结果：Ⅳ型胶原蛋白在3种肿瘤的非黏液性腺癌(NMC)和黏液性腺癌(MC)中的表达均有显著性差异；Ⅳ型胶原蛋白的表达情况与MC的转移相关；与胃和大肠MC相比，乳腺MC中Ⅳ型胶原蛋白的阳性率较高(70％)，而乳腺MC的转移率较低(28．5％)，有显著性差异。结论：乳腺MC较少发生转移可能与其表达较高水平的Ⅳ型胶原蛋白有关。     

采用双峰双波长分光光度法测定木耳中的铅含量

应用双峰双波长分光光度法原理，测定食物中的铅含量，与一般采用的波长双硫腙法相比，消除了干扰成分对测定的影响，大大提高了测定的灵敏度。本法准确度，精密度，回收率符合定量分析要求。     

雌激素和大肠肿瘤

自1916年Lathrop首次报道雌激素对乳腺有潜在致癌性以来,人们陆续发现雌激素不但对靶组织具有致癌或促癌作用,而且对非靶组织,如,淋巴组织、皮肤、脑膜、肠粘膜等亦有类似作用。至于雌激素对大肠肿瘤的发生、发展之影响究竟如何,目前仍众说纷纭,莫衷一是。本     

生物材料生物相容性的分子生物学研究进展

随着分子生物学的迅速发展，生物材料生物相容性研究手段逐渐多样化，其评价已从整体、细胞水平进入了分子水平，本将对生物材料的分子生物相容性研究的现状进行回顾和展望。