吗啡预处理-后处理对大鼠离体心脏缺血再灌注损伤的影响

Objective To evaluate the effects of morphine preconditioning-postconditioning on ischemia-reperfusion (I/R) injury in isolated rat hearts. Methods Male SD rats weighing 180-200 g were killed after intraperitoneal injection of heparin 500 U/kg. The hearts were immediately removed and perfused in a Langendorff apparatus with K-H solution gassed with 95%O2-5%CO2 .HR and left ventricular systolic pressure (LVSP) were measured from a fluid-filled latex balloon in the left ventricle. Global myocardial ischemia was induced by interrupting perfusion for 45 min followed by 60 min reperfusion. Forty isolated rat hearts were randomly divided into 5 groups (n = 8 each): group 1 (I/R); group II morphine preconditioning (M1 ); group Ⅲ morphine postconditioning (M2); group IV M1 + M2; group V 5-hydroxydecanoate (5-HD) + M2. Group M1 was perfused with K-H solution containing morphine 3.0 μmol/L for 20 min 30 min before ischemia followed by 10 min normal K-H solution perfusion. Group M2 was perfused with K-H solution containing morphine 3.0 μmol/L for 10 min at the beginning of reperfusion followed by 50 min normal K-H solution perfusion. Group 5-HD + M2 was perfused with K-H solution containing morphine 3.0 μmol/L+ 5-HD 10-4 mmol/L for 10 min at the beginning of reperfusion followed by 50 min normal K-H solution perfusion. Myocardial CK-MB activity was measured and myocardial infarct size (IS/AAR) detennined (by 2,3,5-triphenyl tetrazolium staining) at the end of 60 min reperfusion. Results The preconditioning, postconditioning and combination of preconditioning and postconditioning with morphine 3.0 μmol/L perfusion for 10 min all provided cardio-protective effects in terms of IS/AAR and myocardial activation of CK-MB. Conclusion Although the combination of morphine preconditioning and postconditioning can protect the heart against I/R injury, the effects are similar to those of either of them alone, and the reason may be that either of them alone protects the heart against I/R injury via activating mitoKATP .     

组合靶基因自动检测仪应用软件的设计

目的：研制与开发组合靶基因自动检测仪应用软件。方法：应用VB6．0编程语言及Access 97数据库实现压电石英谐振频率的实时检测与分析。结果：实现了组合靶基因自动检测仪压电石英谐振频率的实时检测。数据分析，结果保存与数据转换等。结论：组合靶基因自动检测仪应用软件是一类可实时检测与分析压电石英谐振频率的新型实用性软件，可广泛应用于压电石英谐振频率的检测。     

中药治疗荨麻疹65例临床观察

1 引言荨麻疹是皮肤、粘膜血管通透性一时性增加所致。相当于祖国医学的“(疒咅)癗”“隐疹”。其病因为:“风为百病之长,善行数变”,“肺主皮毛,主一身之表”。因此,风邪容于肌肤、皮毛、腠理之间而致。 2 临床资料 2.1 一般资料:65例患者,女性45例,男性20例,以21～30岁者最多,30～40岁者     

责任护士的心理品质与职业道德

责任制护理是以病人为中心进行有计划、有系统地整体身心护理,要做好责任制护理不仅要有过硬的业务技术,还要有良好心理品质和高尚的职业道德。一、责任护士的心理品质特点(一)具备全心全意为病员服务的献身精神,要     

小鼠脑肿瘤与脑外伤后血脑屏障通透性的改变

伊文氏兰与血浆蛋白结合便不能通过血脑屏障，因此可用作定性观察血脑屏障通透性变化。本文用２％伊文氏兰从尾静脉注入小鼠体内，观察到昆明种小鼠脑内接种Ｇ４２２胶质母细胞瘤后，其肿瘤生长部位兰染；切下大块兰色部分，组织学检查证明其为单纯肿瘤组织；残留的兰染边缘完全切下，组织学表现为新生血管丰富的肿瘤生长前缘；切净兰染部分的脑组织经组织学检查无残留瘤细胞。用手术方法造成小鼠脑损伤，可见伤口处组织兰染，自手术后５小时持续到第七天。以上说明小鼠患脑恶性肿瘤与脑外伤后，血脑屏障开放且有其规律。     

丹参防治爆震性聋的实验研究

观察了爆震后实验动物的听性脑干反应(ABR)和氧自由基代谢产物丙二醛(MDA)含量变化。结果发现,单纯爆震组ABR阈移和耳蜗MDA含量比丹参防治组和正常对照组明显增高;丹参防治组与正常对照组相比无显著差异。提示丹参能较有效地防治爆震性耳聋,并对其作用机理进行了讨论。     

急性脓胸86例治疗体会

我院从1985年6月～1993年10月共收治急性脓胸86例,现总结分析如下。1.临床资料 本组共86例,男49例,女37例,年龄最小的57天,最大60岁,其中13岁以下小儿46例,占53.5％。左侧脓胸36例,右侧脓胸42例,双侧脓胸8例。其中液气胸42例。 脓胸继发于肺部感染48例,继发于化脓性心包炎4例,败血症引起者6例,结核性脓胸5例,肺部手术后5例,食管切除术后7例,继发于咽部脓肿1例,胸部外伤6例,病