保定市儿童医院2 726例肺炎患儿多种病原检测与临床研究

目的了解保定地区小儿肺炎的病毒、细菌、肺炎支原体检出阳性率及不同季节、不同地域检出率之间的关系。方法2003年1月～2006年1月选择保定儿童医院2 726例肺炎患儿,应用酶联免疫吸附实验(ELISA)技术检测血清病毒IgM抗体和肺炎支原体IgM抗体,同时作痰细菌培养。结果病毒检出率47.76%(1 302/2 726);细菌检出率13.79%(376/2 726);肺炎支原体检出率20.73%(565/2 726);病毒于第一、四季度为感染高峰;细菌第一、三季度感染率高,肺炎支原体第二、三季度感染率高。病毒感染城乡差异无统计学意义(P>0.05),细菌感染农村高于城市,肺炎支原体感染城市高于农村,两组间的差异均有统计学意义(P<0.05)。结论保定地区肺炎患儿仍以病毒感染为主,其次为肺炎支原体及细菌感染。病毒感染冬春季发病率高;细菌第一、三季度感染率高,农村高于城市;肺炎支原体第二、三季度感染率高,城市高于农村。     

ISSR-PCR鉴别绞股蓝属7种植物

目的 采用ISSR-PCR技术对绞股蓝属(Gynostemma BI.)绞股蓝G.pentaphyllum、五柱绞股蓝G.pentagynum、心籽绞股蓝G.cardiospermum、长梗绞股蓝G.longipes、喙果绞股蓝G.yixingense、疏花绞股蓝G.laxiflorum、广西绞股蓝G.guangxiense 7种药用植物进行DNA分子水平鉴定.方法 CTAB法提取绞股蓝属植物总DNA,以57条ISSR引物进行PCR扩增及琼脂糖凝胶电泳分析.结果 筛选出产物清晰稳定的14条引物,其中引物UBC-873与UBC-895具有较高的多态性条带比率,均可独立区分7种绞股蓝属植物.结论 ISSR-PCR分析可有效区分鉴别常见绞股蓝属植物.     

热性惊厥患儿血清褪黑素水平和细胞免疫功能的变化及其临床意义

Objective To investigate the changes of melatonin and cellular immunological function in children with febrile seizures and its clinical significance. Methods 50 children, including 23 cases with complex febrile seizure (CFS) and 27 cases with simple febrile seizure (SFS) , and 25 cases with upper respiratory infections children selected as control group were enrolled in this study. Serum melato- nin was measured by enzyme-linked immunosorbent assay (ELISA) and cellular immunological function was measured by flow eytomcter. Results The levels of serum melatonin in the 3 groups of CFS, SFS, control were(14. 91±2. 61) ng/L, (20. 72±2. 54) ng/L, (23.93± 2. Ol) ng/L, respectively. The melatonin levels in CFS children were significantly decreased than that in control group and SFS children (P <0. O1). CD3 + ,CD4 +, the ratio of CD4 + /CD8 + and CD8 + in CFS group were significantly decreased than that in control group and SFS group (P <0.01). The ratio of CD4 +/CD8 + in SFS group was significantly decreased than that in control group (P <0.05), but CD3 + ,CD4 + and CD8 + had no statistics significance among these groups(P >0. 05). The serum rnelatonin level were positive related withdecreaseddegreeofCD3+,CD4+ andtberatioofCD4+ /CDS+ (r≥0. 472, P <0.05). Conclusion The disorder cfcellular immunological function was possible related with the loss of serum melatonin, and the loss of serum melatonin maybe one of the reasons for febrile seizures relapse and brain injured.     

脑梗塞部位对肢体功能康复影响的比较分析

目的:比较分析不同部位脑梗塞对脑梗塞患者肢体功能康复的影响。方法:采用Barthel指数(BI)与美国国立卫生研究院卒中量表(NI HSS)对2006年入住青岛市李沧区第三医院的64名首次发病的脑梗塞患者治疗前后进行评估,比较左侧半球脑梗塞、右侧半球脑梗塞、双侧半球和/或脑干梗塞3组患者Barthel指数与NI HSS量表评估得分。结果:3组患者经治疗后,Barthel指数与NI HSS量表评分较治疗前均明显改善,均P<0.05,但Barthel指数与NI HSS量表评分的改善3组之间比较无显著性差异(P>0.05)。结论:不同部位脑梗塞患者经治疗后肢体运动功能及日常生活活动能力均有改善。     

Differential retention of alpha-vitamin E is correlated with its transporter gene expression and growth inhibition efficacy in prostate cancer cells

BACKGROUND: Epidemiological studies showed Vit E has protective effects against prostate cancer (PCa). Interestingly, different prostate cancer cells have different sensitivity to alpha-Vit E or VES treatment. The goal of this study is to determine whether cellular Vit E bioavailability and its transport proteins are important contributing factors. METHODS: alpha-Vit E and its ester form, VES, were used to treat prostate cancer LNCaP, PC3, and DU145 cells, and their growth rates were determined by MTT assay. Cellular levels of Vit E were quantified using HPLC as the index of bioavailability. The expression levels of Vit E transport proteins were determined by real-time PCR. RESULTS: Among these PCa cells, only LNCaP cells were sensitive to 20 microM alpha-Vit E treatment, while both LNCaP and PC3 cells were sensitive to 20 microM VES treatment. Coordinately, cellular levels of alpha-Vit E and VES positively correlated to their inhibitory effects. Further study found expression levels of Vit E transport proteins, including tocopherol associated protein (TAP), scavenger receptor class B type I (SR-BI), alpha-tocopherol transfer protein (TTP), and ATP binding cassette transporter A1 (ABCA1), were different in various PCa cells, which may contribute to cellular Vit E bioavailability. This notion is further supported by the findings that overexpression or knockdown of TTP could coordinately alter cellular alpha-Vit E levels in PCa cells. CONCLUSION: Antiproliferative efficacy of alpha-Vit E is correlated with its cellular bioavailability in PCa cells. Modulating the expression of the efflux or influx transporters could sensitize the growth inhibition efficacy of Vit E in prostate cancer cells.     

A randomized clinical trial of dietary calcium to improve bone accretion in children with juvenile rheumatoid arthritis

OBJECTIVE: To examine a behavioral intervention (BI) to increase calcium intake in children with juvenile rheumatoid arthritis (JRA) on calcium intake and bone mass 6 and 12 months after treatment. STUDY DESIGN: A randomized trial compared a 6-session BI to a 3-session enhanced standard of care (ESC) with 49 children ages 4 to 10 years with JRA. Calcium intake was assessed via 3-day diet diaries. Total body bone mineral content (BMC), arms and legs BMC, and lumbar spine bone mineral density were assessed by dual energy x-ray absorptiometry. RESULTS: BI maintained an average calcium intake of 1500 mg/d at 6- and 12-month follow-up. This was greater than their baseline level of 972 mg/d, but not greater than the intake of 1300 mg/day maintained by ESC (P=.09). The BI had a 4% and 2.9% greater gain in total body bone mineral content than ESC at 6 and 12 months, respectively (P=.005), and a 7.1% and 5.3% greater gain in arms and legs BMC at 6 and 12 months than ESC (P=.0007). CONCLUSIONS: BI is effective in increasing calcium intake and BMC in children with JRA over a 12-month period.     

Comparison of different cellular models measuring in vitro the whole human serum cholesterol efflux capacity

BACKGROUND: Fu5AH rat hepatoma cells and cAMP (cyclic AMP)-pretreated J774 mouse macrophages are commonly used as models for SR-BI (scavenger receptor class B type I) and ABCA1 (ATP binding cassette transporter 1)-mediated free cholesterol efflux to whole serum, respectively. However, the responsiveness of Fu5AH, control or cAMP pretreated J774 cells to the various lipids and HDL (high-density lipoprotein)-parameters from both normo- and dyslipidaemic subjects has never been compared within the same study. MATERIALS AND METHODS: Fifty-eight men were classified into four groups: type IIa hypercholesterolaemic (n = 12), type IIb dyslipidaemic (n = 13), type IV hypertriglyceridaemic (n = 18) and normolipidaemic (n = 15) were recruited. A complete lipid profile including prebeta-HDL was performed. Cholesterol efflux from Fu5AH cells as well as from control or cAMP pretreated J774 cells were measured; the difference between these two latter values being taken as the ABCA1-mediated efflux. RESULTS: The Fu5AH and the control J774 cells delivered cholesterol to mature HDLs, especially to phospholipid (PL)-rich HDL. Using cAMP pretreated cells, the ABCA1-dependent efflux was highly sensitive to prebeta-HDL, which appeared to be a factor in determining the efflux. Consistent with the dependence of the SR-BI-mediated efflux on HDL-PL levels, which are not different between groups, all sera displayed similar efflux capacities from the Fu5AH cells. Conversely, in accordance with their high prebeta-HDL levels, the ABCA1-dependent efflux highlighted the efficiency of type IV sera. CONCLUSION: Two complementary cellular models providing SR-BI and ABCA1-dependent efflux should be used to measure the capacity of a biological fluid which contains a wide variety of components to promote cholesterol efflux.     

天麻有效成分制备与测定简易方法的探讨

目的探讨简单易行的天麻有效成分提取与测定的方法。方法将天麻用70％乙醇加热回流法制备天麻提取物，高效液相色谱法测定天麻素含量，并用紫外分光光度法测量，制天麻素含量-吸光度曲线。结果用70％乙醇加热回流法提取所得天麻提取物中天麻素含量为1．6mg／mL，紫外分光光度法测定天麻素含量在100μg／mL-300μg／mL范围与吸光度（A224nm）呈线性关系（天麻素含量Cμg／mL=-688．317＋286．000×A224nm，t=5．690，p〈0．05）。结论采用70％乙醇加热回流法制备天麻提取物，并用紫外分光光度法测定其中天麻，素含量，方法简单易行，成本低廉。     

热性惊厥患儿血清褪黑素水平和细胞免疫功能的变化及其临床意义

Objective To investigate the changes of melatonin and cellular immunological function in children with febrile seizures and its clinical significance. Methods 50 children, including 23 cases with complex febrile seizure (CFS) and 27 cases with simple febrile seizure (SFS) , and 25 cases with upper respiratory infections children selected as control group were enrolled in this study. Serum melato- nin was measured by enzyme-linked immunosorbent assay (ELISA) and cellular immunological function was measured by flow eytomcter. Results The levels of serum melatonin in the 3 groups of CFS, SFS, control were(14. 91±2. 61) ng/L, (20. 72±2. 54) ng/L, (23.93± 2. Ol) ng/L, respectively. The melatonin levels in CFS children were significantly decreased than that in control group and SFS children (P <0. O1). CD3 + ,CD4 +, the ratio of CD4 + /CD8 + and CD8 + in CFS group were significantly decreased than that in control group and SFS group (P <0.01). The ratio of CD4 +/CD8 + in SFS group was significantly decreased than that in control group (P <0.05), but CD3 + ,CD4 + and CD8 + had no statistics significance among these groups(P >0. 05). The serum rnelatonin level were positive related withdecreaseddegreeofCD3+,CD4+ andtberatioofCD4+ /CDS+ (r≥0. 472, P <0.05). Conclusion The disorder cfcellular immunological function was possible related with the loss of serum melatonin, and the loss of serum melatonin maybe one of the reasons for febrile seizures relapse and brain injured.     

Non-sensitising air pollution at workplaces and adult onset asthma

Objective The aim of this study was to elucidate further whether occupational exposure to non-sensitising air pollution at workplaces increases the risk of adult onset asthma.Methods One hundred and twenty persons with asthma diagnosed by general practitioners, aged 20–65 years, were compared with 446 referents matched for age and gender and living in the same community as the cases. Information about occupation, exposure to specific allergens, smoking habits, dwellings and atopy was obtained from a postal questionnaire. The subjects' occupations were categorised as clean or polluted, based on the judgement of the referents on their respective occupations.Results Three years or more of work in air-polluted workplaces resulted in an odds ratio of 1.7 (95% confidence interval 1.0–2.7). Stratification of the material on smoking habits, gender or atopy did not alter the results, nor did exclusion of subjects exposed to specific allergens of statistical significance in this material, e.g. flour dust. Smoking per se did not bring any risk of asthma. Working in buildings affected by dampness and mould brought a fourfold significant risk.Conclusion In this study occupational exposure to unspecific air pollution at workplaces was associated with an increased risk of adult-onset asthma.