在输液同侧采血致患者贫血误输红细胞一例分析

1病例简介 患者，男，17岁，2009年12月21日因从二偻摔下致脾损伤，入我院外科抢救室。 2血常规。血型检查     

流式细胞术测定红细胞存活率的方法建立

目的 :建立应用流式细胞术测定红细胞体内存活率的方法。方法 :献血者Rh表型ccDEE ,受血者为CCDEE ,以抗 c为一抗结合ccDEE的红细胞 ,两群细胞按设定的比例混合 ,以山羊抗人标记荧光素的IgGab段为二抗连接抗 c ,经流式细胞仪测定ccDEE表现型红细胞在两群细胞中所占的比例 ,并与实际的比例进行相关性比较。结果 :已知两群细胞数的比例与流式细胞仪测定的比例的相关系数r =99.87。结论 :流式细胞术技术可以体外测定红细胞体内的存活率     

广州市番禺区学龄前儿童各年龄组间RBC、HGB及MCV比较

目的：探讨广州市番禺地区学龄前儿童各年龄组间红细胞计数(RBC)、血红蛋白浓度（HGB）及平均红细胞体积(MCV)的参考值范围。方法选择2014年在番禺区中心医院就诊的6个月≤年龄≤6岁的健康儿童3290例;筛除离群数据后,按性别、年龄分组,采用SPSS19.0软件进行统计分析。结果除了女童不同年龄组间及2~3岁男女儿童间RBC水平、6个月~1岁及4~6岁男女童间HGB水平、2~3岁男女童间MCV水平比较差异均无统计学意义(P<0.05)外,其他参数在不同年龄组间比较差异均有统计学意义(P<0.05);学龄前儿童各参数与《全国临床检验操作规程》(第4版)参考范围比较,差异均有统计学意义(P<0.05)。结论番禺地区应根据本地域人群特点、不同年龄及性别建立合适RBC、HGB、MCV参考值范围,为临床诊断儿童贫血和评价儿童的营养和生长发育状况提供可靠的依据。     

运用甘油磷酸氧化酶法测定冰冻红细胞解冻后残留甘油的含量

目的 建立测定冰冻红细胞解冻洗涤后残留甘油含量的酶学方法测定工艺.方法 进行甘油磷酸氧化酶法的线性范围测定、重复性、准确性(回收试验)、游离血红蛋白干扰试验,并比较不同样本处理方法(钨酸钠裂解法、三氯醋酸裂解法及制备后2h直接取上清液法)对酶法检测甘油的影响;进行系列浓度甘油红细胞酶法测定结果与传统过碘酸钠滴定法测定结果比对.结果 用GPO-PAP法检测甘油浓度在0.05～2.0 g/L时有较好的线性关系,,=0.9994,Y=0.942 X+0.0239;甘油含量为0.2 g/L、1.0 g/L的样本,批内CV值分别为2.68％、0.81％(n=20),日间CV值分别为4.59％、4.37％(n=20);甘油浓度从0.29～2.09 g/L间其回收率97.1％～100.8％,样本的平均回收率为99.0％;10g/L游离血红蛋白对GPO-PAP法检测结果无干扰;3种样本处理方法所测得的系列浓度甘油红细胞样品及解冻去甘油红细胞制品(n=18)酶法测定结果一致(P＞0.05);系列浓度甘油红细胞测定结果显示酶法测定比滴定法更准确,滴定法测定值偏高.结论 甘油磷酸氧化酶法可用于冰冻解冻去甘油红细胞制品中残留甘油含量的测定,方法简便、快速、准确、重复性好.3种样本处理方法对GPO-PAP法检测甘油含量无影响.     

血清学诊断 ABO 新生儿溶血病的实验研究

目的：了解长沙地区 ABO 血型新生儿溶血病（HDN）的发病情况,探讨血清学三项实验在诊断新生儿溶血病中的临床价值,为该病的早期预防、早期诊断和临床治疗提供依据。方法：收集中南大学湘雅医院2016年1月~7月产科和新生儿科疑似母婴 ABO 血型不合的新生儿血液标本,对其进行 ABO 血型检测及新生儿溶血病血清学三项试验（直接抗人球蛋白试验、游离抗体试验和红细胞抗体释放试验）。结果：在583例检测结果中,确诊为新生儿溶血病的有145例,发病率为24.87%。其中男性患儿80例,发病率为25.64%,女性患儿65例,发病率为23.98%,差异无统计学意义;A 型血患儿90例,O-A 型母婴血型模式的发病率为54.88%,B 型血患儿55例,O-B型母婴模式的发病率为41.35%,差异显著。145例 HDN 中直接抗人球蛋白试验阳性38例,阳性率26.21%,游离抗体试验阳性76例,占52.41%,红细胞抗体释放试验阳性144例,阳性率为99.31%,三者比较存在着显著的差异性。结论：长沙地区 ABO 新生儿溶血病发病率为24.87%,男性新生儿和女性新生儿的发病率无差异性,O-A 母婴血型发病率高于 O-B 母婴血型。血清学三项试验是临床最常用的诊断新生儿溶血病的方法,其中红细胞抗体释放试验敏感度最高,游离抗体试验次之,直接抗人球蛋白试验敏感度最低。血清学诊断为 ABO 新生儿溶血病的早期预防、早期诊断和临床治疗提供依据。     

The role of non-specific lymphocytes in antigen-induced proliferative response in vitro

The antigen-induced proliferative response of lymph node cells from immunized mice is proportional to the number of primed lymphocytes in the microtiter wells. Addition of normal lymphocytes did not alter the magnitude of the antigen-specific [³H]TdR uptake of immune lymph node cells. In contrast, normal lymphocytes increased the antigen-specific thymidine incorporation of enriched populations of antigen-specific lymphocytes. This enhancing effect was especially pronounced, and proportional to the number of supplementing unsensitized lymphocytes, with a small cell number of enriched lymphocytes, where the specific responsiveness could barely be detected without addition of normal lymphocytes.Enriched populations derived through adherence to antigen-pulsed macrophages (‘selected’ cultures) as well as those obtained by growing immune lymph node cells with antigen-pulsed macrophages (‘supernatant’ cultures) had an improved proliferative response after addition of normal lymphocytes. However, the proliferative response of the ‘selected’ cultures was extremely dependent on normal lymphocytes as they could express only 10% of their full stimulatory capability in the absence of the latter. This indicates that the blastogenic signal delivered by the activated specific T cells recruits normal lymphocytes which do not adhere to the antigen-pulsed macrophages. Under the same conditions the recruiting signal is incapable of inducing the multiplication of antigen-sensitized cells.The potential recruitable uncommitted lymphocytes are present in excess among immune lymph node cells, while depleted from the ‘selected’ cultures. The enriched ‘supernatant’ cultures seem to contain considerable numbers of recruitable lymphocytes although they are present in quantities less than are required to provide for the full amplifying signal.This meaning of these findings for the evaluation of enrichment of antigen-specific T cells and of antigen-induced response based on [³H]TdR uptake is discussed.     

Chemotactic factor concentration gradients in chemotaxis assay systems

In most assays of chemotaxis the gradient of the chemotactic factor is established and later destroyed by its diffusion through some matrix. The characteristics of the gradient depend upon the geometry of the assay system, the diffusion coefficient of the chemotactic factor and the concentration of the chemotactic factor added. We have solved the diffusion equations to characterize the gradients present in 3 assays of ceemotaxis in current use: the millipore, under-agarose and visual assay systems. In each case the solutions are presented for various assay times and for chemotactic factors with various diffusion coefficients.