ASPM基因在氯化镉致16HBE细胞氧化损伤中的作用

目的研究ASPM基因在氯化镉(CdCl_2)致人支气管上皮细胞(16HBE)氧化损伤中的作用。方法分别用0、10、20和30μmol/L CdCl_2处理16HBE细胞24 h,以及用30μmol/L CdCl_2处理16HBE细胞12、24和48 h。利用qRT-PCR法检测16HBE细胞ASPM基因表达变化情况;采用慢病毒稳定表达技术构建ASPM缺陷细胞株;采用硫代巴比妥酸(TBA)法检测各组细胞内丙二醛(MDA)含量,用水溶性四唑盐法检测细胞中超氧化物歧化酶(SOD)活力。结果与空白对照组(未用CdCl_2处理的16HBE细胞)相比,16HBE细胞随着CdCl_2染毒剂量与时间的增加,SOD活性出现降低,MDA出现增加的趋势;与空白对照组(未用CdCl_2处理的16HBE细胞)相比,实验组ASPM mRNA相对表达量与CdCl_2呈现出时间-剂量关系(P0.05);ASPM基因低表达细胞株构建的基因沉默率为96.9%(P0.05);与相同CdCl_2剂量点和时间点处理的16HBE细胞相比,ASPM缺陷细胞的SOD含量低于16HBE细胞,而MDA含量高于16HBE细胞(P0.05)。结论 ASPM基因缺陷细胞加剧CdCl_2所致氧化损伤,提示ASPM基因在CdCl_2致16HBE细胞氧化损伤过程中发挥拮抗作用。     

依托社会团体开展健康教育对2型糖尿病患者生存质量的影响

目的 探讨依托社会团体,建立会员制,开展健康教育,对2型糖尿病患者生存质量的影响.方法 随机选择加入社会团体成为会员的2型糖尿病患者110例为干预组,再随机选择非社会团体会员就诊的2型糖尿病患者113例为对照组.干预组患者接受健康家园团体组织的管理与教育,对照组进行常规的健康教育.采用SF-36生存质量量表进行生存质量评估,随访半年后再次评分.结果 干预前2组间各维度评分比较差异无统计学意义.半年后随访,对照组各维度评分与半年前比较无明显改善.干预组在总体健康、生命活力、情感职能、精神健康及社会功能等维度评分均有明显升高,差异有统计学意义.结论 成立社会团体,开展健康教育,可以提高2型糖尿病患者的生存质量.     

软骨细胞与骨髓基质细胞共培养体外构建软骨组织的实验研究

Objective To investigate the feasibility of chondrogenesis in vitro with bone marrow stromal cells (BMSCs) induced by the co-cultured chondrocytes. Methods The BMSCs and chondrocytes were separated from pig and cultured. The supernatant of chondrocytes was used as the inducing solution for BMSCs from the 2nd generation. 7 days later, samples were taken and underwent immunohistochemistry and RT-PCR for detection of the expression of specific type Ⅱ cartilage collagen,type Ⅱ collagen and aggrecan mRNA. The cultured BMSCs and chondrocytes were mixed at a ratio of 8:2(BMSC: cartilage cell) and were inoculated into a polyglycolic acid/polylactic acid (PGA/PLA) scaffold at the final concentration of 5.0 × 107/ml. The cartilage cells and BMSCs were also inoculated seperately at the same concentration as the positive and negative control. Pure cartilage cells at 20% of the abovementioned concentration (1.0 × 107/ml) were used as the low concentration cartilage cell control group. Samples were collected 8 weeks later. General observations, wet weight, glycosaminoglycans (GAGs) determination and histological and immunohistochemistry examinations were performed. Results The expression of type Ⅱ collagen, type Ⅱ collagen and aggrecan mRNA were positive in induced BMSCs.In the co-cultured group and the positive control group, pure mature cartilage was formed after 8 weeks of culture in vitro, and the size and shape of the scaffold were maintained. The newly formed cartilage in the two groups were almost the same in appearance and histological properties. The immunohistochemistry results indicated that the cartilage cells of the two groups all expressed ample cartilage-specific type Ⅱ collagen. The average wet weight and GAG content in the co-cultured group reached more than 70% of those in positive control group. Only an extremely small amount of immature cartilage tissues was formed in local regions in pure BMSC group, and the scaffold was obviously shrunk and deformed. Although the wet weight of newly generated cartilage tissue in the low concentration cartilage cell group reached 30% of that in positive control group, the scaffold was obviously shrunken and deformed. Only regional and discontinuous cartilage tissues were formed, and the amount of newly formed cartilage was obviously less than that in the co-culture group and the positive control group. Conclusions Chondrocytes can provide a micro-environment for the formation of cartilage, and also effectively induce BMSC to differentiate into chondrocytes and form tissue-engineered cartilage in vitro.     

内脂素和脂联素与中青年2型糖尿病患者骨密度及骨代谢关系的研究

目的探讨内脂素、脂联素与中青年2型糖尿病(T2DM)患者骨密度(BMD)及骨代谢的关系。方法收集2010年1月—2012年1月在我院就诊的中青年2型糖尿病患者127例(T2DM组),同期将在我院体检中心及课题组社区进行流行病学调查检查血糖正常的健康者120例(对照组),两组年龄均为20～40岁。比较两组受检者的年龄、腰臀比及体质指数(BMI),采用酶联免疫法(ELISA法)测定血清内脂素、脂联素水平,双能X线骨密度仪测定腰椎(L2～L4)及左股骨颈BMD,ELISA法检测血清骨钙素(BGP)及Ⅰ型胶原胶联蛋白羧基肽(CTX-Ⅰ)。采用SPSS 13.0统计软件进行统计学处理,组间比较采用两独立样本t检验,两因素间的关系采用Pearson相关分析。结果 (1)两组受检者的BMI及腰臀比比较,差异均无统计学意义(P>0.05);而T2DM组血清内脂素水平、腰椎(L2～L4)及左股骨颈BMD高于对照组,而脂联素、BGP、CTX-Ⅰ水平则低于对照组,差异均有统计学意义(P<0.05)。(2)相关分析显示,BMI、腰臀比、内脂素均与腰椎及左股骨颈BMD呈正相关(P<0.05),与BGP、CTX-Ⅰ呈负相关(P<0.05);而脂联素与腰椎及左股骨颈BMD呈负相关(P<0.05),与BGP、CTX-Ⅰ呈正相关(P<0.05)。结论血清内脂素、脂联素水平与中青年T2DM患者骨代谢、腰椎及左股骨颈BMD相关。     

蛛网膜囊肿-腹腔分流术后两种罕见并发症一例

患儿男,4岁.因"头颅增大、左眼斜视4年"入院.查体:患儿智力正常,头围55 cm,左眼睑下垂,眼球轻度突出、内斜,向上、向下及外展运动受限,瞳孔直径略大于右侧,对光反应灵敏,左眼视力0.1、右眼视力0.3,视野正常.感觉、肌力、共济运动等均正常.CT见左额颞大片状液性低密度区,大小约111 mm×63 mm×110 mm,脑实质受压,侧脑室变形,中线结构右偏.诊断为左额颞巨大蛛网膜囊肿,脑发育异常.在全麻下行囊肿-腹腔分流术,术中见囊液清澈透亮.术后正常出院.术后第13个月,发现患儿右侧阴囊肿大,阴囊内可触及条状物,腹部X线提示分流管末端疝人阴囊(图1),请普外科会诊考虑并发右腹股沟斜疝,拟入院行疝修补及分流管调整术.     

悬雍垂腭咽成形术治愈阻塞性睡眠呼吸暂停综合征合并遗尿症一例

我们采用悬雍垂腭咽成形术 (ＵＰＰＰ)治疗阻塞性睡眠呼吸暂停综合征 (ＯＳＡＳ)的同时治愈了遗尿症 1例 ,现报告如下。患者男性 ,4 3岁 ,已婚。因打鼾憋气 3年于 1998年 3月17日入院。 3年前睡眠时出现打鼾 ,且频频憋气 ,经常憋醒 ,作恶梦 ,四肢骚动 ,晨起头痛头晕 ,白天嗜睡 ,精神不集中 ,记忆力减退。自发病后开始尿床 ,初起为间断性 ,近 2年来每天于夜间睡眠中均尿床 1～ 3次 ,从未间断过 ,晚上少饮水或不饮水夜间也尿床 ,在日间清醒状态下能控制排尿 ,无尿频、尿急、尿痛 ,曾多次去泌尿科检查 ,排除了糖尿病、尿崩症、肾炎、神经系统等…     

Bc1-6与弥漫性大B细胞淋巴瘤

弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma DLBCL)是一组在形态学、遗传学和临床表现上都具有异质性的肿瘤。在2001年WHO关于淋巴造血系统的最新分类中，DLBCL被定义为：B细胞起源的、有大的肿瘤细胞、具有侵袭性临床表现，需要高效力的化学治疗的一组恶性淋巴瘤。这类肿瘤发生于结内或结外，可原发或继发于其它低度恶性淋巴瘤的演进。DLBCL发病的具体机制不清，但Bc1-6基因与其关系较密切，可能与DLBCL发生、发展及预后相关，近年来一直成为研究的热点。     

烧伤合并吸入性损伤并发急性呼吸窘迫综合征八例

1 临床资料 2004年10月-2007年5月,笔者单位收治特重度烧伤合并中重度吸入性损伤并发急性呼吸窘迫综合征(ARDS)患者8例,均为男性,年龄22～60岁.火焰烧伤3例、蒸气烫伤2例、热液烫伤2例(其中1例为癫痫发作后跌入热水池中烫伤)、瓦斯爆炸伤1例.烧伤总面积45%～100%,其中Ⅲ度25%～70%TBSA.8例患者伤后4～12 d并发ARDS.