DNA methylation profiling of ovarian carcinomas and their in vitro models identifies HOXA9, HOXB5, SCGB3A1, and CRABP1 as novel targets

Background  

The epigenetics of ovarian carcinogenesis remains poorly described. We have in the present study investigated the promoter methylation status of 13 genes in primary ovarian carcinomas (n = 52) and their in vitro models (n = 4; ES-2, OV-90, OVCAR-3, and SKOV-3) by methylation-specific polymerase chain reaction (MSP). Direct bisulphite sequencing analysis was used to confirm the methylation status of individual genes. The MSP results were compared with clinico- pathological features.     

超声波提取洋葱总黄酮及鉴别

目的:为充分利用洋葱植物资源,避免资源的浪费,探讨洋葱总黄酮的提取及鉴别方法。方法:采用超声波乙醇浸提法从洋葱中提取黄酮类物质,对所提取的黄酮类物质进行验证,并用紫外分光光度法测定含量。结果:测得样品中总黄酮的含量C=20.58%,平均回收率为102.1%,其纯度和产率均较高。结论:该方法采用全物理过程,无任何污染,是提取洋葱黄酮类物质的有效途径。     

无髓牙冠内漂白方法的对比研究

目的探讨无髓牙内漂白技术中2种细节操作方法对漂白效果影响。方法选择口腔中前牙和双尖牙区至少2颗变色无髓牙的患者40例,对2颗牙分别采用方法Ⅰ(标准根管治疗后,用光固化玻璃离子封闭根管口,用常温下浸有30%过氧化氢的棉球封闭在髓腔内,3d复诊1次,共封药3次后进行充填治疗)和方法Ⅱ(标准根管预备后,根充前用加热到36℃的30%过氧化氢反复冲洗髓腔,然后做常规根充和充填治疗)治疗。2组均采用同种复合树脂严密充填,1 a后用相同方法观察美观效果和强度效果。结果方法Ⅱ美观效果与强度效果均明显优于方法Ⅰ(P<0.05)。结论漂白变色的无髓牙最好方法是即刻高效的热漂白方法。     

水解猪全血蛋白提取氨基酸混合物的营养评价

猪全血经酸水解及离子交换树脂分离提取,适当补充L-色氨酸,L-蛋氨酸及L-异亮氨酸的混合氨基酸粉(简称配方Ⅰ)经蛋白质营养效价的研究结果表明,混合氨基酸配方Ⅰ,必需氨基酸含量为49.33％,其组成模式接近酪蛋白或全鸡蛋蛋白质,第一限制氨基酸为含硫氨基酸,化学分(CS)为63.77,必需氨基酸指数(EAAI)为92.78,均高于酪蛋白,大鼠生长试验证明,体重增长,蛋白质效能比(PER)和净蛋白比(NPR)与酪蛋白比较均无显著差别(P>0.05),但优于猪血粉(P<0.001)。本品可做为营养要素膳的良好氮源,又可以用于食品添加,为猪血的综合利用提供了一个重要途径。     

应用K-ras基因突变诊断胰腺癌的探讨

目的 采用连续富集酶切一限制性片段长度多态性／聚合酶链反应（CED-RnP／PCR）探索K-ras基因在胰腺癌患者血浆中的表达。方法 以血浆代替以往的标本并采用CED-RFLP／PCR技术检测胰腺癌、非胰腺癌患者及健康对照者血浆K-ras基因突变情况。结果 胰腺癌患者血浆K-Fas基因的突变阳性率为73％，无假阳性率，高于胰液、十二指肠液的检测率，低于细针穿刺的检测率；胰腺良性病变患者及健康对照者血浆中未检测到K-ras基因的突变；胰腺癌组K-ras基因突变与非胰腺癌组及健康对照组相比具有显著性差别。结论 CED-RFLP／PCR法检测血浆K-ras基因突变技术具有简单、易行高效的特点，克服以往技术的弊端，对胰腺癌的诊断及鉴别有一定的应用价值。     

康胃方对新生大鼠HP感染的根除及胃黏膜保护作用

目的 :HP悉尼菌株灌胃复制感染动物模型 ,观察康胃方对HP的根除及胃黏膜细胞保护作用。方法 :SD新生大鼠随机分为正常对照组、病理模型组、三联治疗组及康胃方组 ,除正常对照组外 ,每日经口接种 10 9CFU mlHP菌液 0 1ml,共 3次。 8周后进行UBT ,随即接受相应的治疗 ,共 4周。疗后第 4周 ,测定胃黏膜RO、NO、iNOS、MnSOD及NPSH的含量或活性。结果 :HP感染动物 ,UBT与胃黏膜所含RO、NO、iNOS和MnSOD明显升高 ,NPSH显著下降 (P <0 0 1)。康胃方治疗后 ,UBT降低 ,其他指标明显改善 (P <0 0 1)。结论 :康胃方对新生大鼠感染HP有明显的根除作用 ,并对胃黏膜有一定的细胞保护作用。     

锁定加压钢板治疗骨盆骨折的初步体会

目的探讨锁定加压钢板在骨盆骨折中的应用。方法应用锁定加压钢板治疗8例骨盆后环损伤患者,其中男4例,女4例;年龄24-57岁,平均39岁。致伤原因:全部为车祸伤。按照Tile分型:B1型3例,B2型2例,C1型2例,C2型1例。结果8例患者获9个月到2年的随访。手术时间1.5小时到2个小时。术中无输血。术后一例患者出现褥疮,切口均一期愈合。下肢运动、感觉均正常,无神经损伤表现。结论锁定加压钢板治疗骨盆骨折,尤其对后环的固定具安全、微创、稳定的优势。     

Tuberculin reactivity in adults after 50 years of universal bacille Calmette-Guerin vaccination in Taiwan

We aimed to assess whether tuberculin reactivity in adults is affected by bacille Calmette-Guerin (BCG) vaccination after 50 years of universal BCG vaccination with 80-95% coverage. A community-based study on tuberculin reactivity in 619 participants was conducted in February 2000 in Keelung city, Taiwan. Information on BCG vaccination policies and annual risk of infection (ARI) in the underlying population was extracted from consecutive national prevalence surveys relating to the period 1952-1997. Compared with the expected ARI estimate, the standardized morbidity ratio of positive tuberculin response for vaccination in infancy was 2.2 (95% CI 0.3-15.5) for those aged <10 years. The corresponding figures for older age groups ranged from 3.6 (95% CI 2.2-5.9) for those aged 10-12 years to 0.7 (95% CI 0.5-0.9) for those aged 57-67 years. This suggests that the effect of BCG vaccination on positive tuberculin response in adults aged >30 years is probably negligible irrespective of age at vaccination or revaccination and that the tuberculin skin test can be used to diagnose TB in control programmes in countries with moderate or high incidence of TB.     

Incidence of tuberculosis in mountain areas and surrounding townships: dose-response relationship by geographic analysis

PURPOSE: The incidence of tuberculosis (TB) in Taiwan is known to be high in aboriginal mountain areas and low in the surrounding non-mountain areas. The aim of this study was to assess whether TB incident cases in the surroundings of mountain areas decreased with distance from foci of mountain areas. METHODS: The mountain areas in Taiwan are adjacent to each other and divided into four geographic groups. Townships with high TB incidence in each group were treated as the foci of TB infection. The surrounding townships were then classified, by distance away from foci, into three levels: < 20 km, 20 to 40 km, and 40 to 60 km. Data were obtained on a total of 84,366 TB cases from 1991 to 1997 from the Taiwan Tuberculous Disease Registry Center. RESULTS: The incidence of TB in each of the four groups was significantly higher in the mountain areas compared with non-mountain areas, with relative risks ranging from 8.4 (95% confidence interval [CI], 7.8-9.1) for the southern group to 15.0 (95% CI, 13.4-16.9) for the northern group. Relative risks for surrounding townships decreased with distance in all four groups. Such a gradient relationship was statistically significant. CONCLUSIONS: A significant dose-response relationship between distance from townships with a high incidence of TB and the incidence of TB in the surrounding areas has been demonstrated.