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1.
持续性异位妊娠的诊断及治疗   总被引:34,自引:0,他引:34  
Liu Z  Sun Z  Yang J 《Zhonghua yi xue za zhi》2001,81(20):1261-1263
目的 探讨持续性异位妊娠的发生机理和诊治。方法 回顾了1995-2000年5年间经腹、经腹腔镜手术的411例异位妊娠患者,用logistic回归分析将手术成功与不成功发生持续性异位妊娠患者的两组临床情况进行了比较。结果 7例持续性异位妊娠患者均发生在经腹腔镜手术后,发生率为3.5%。7例中6例发生在输卵管造口等保守性手术后,1例发生在输卵管切除术后。7例中2例因再次出现腹痛、腹腔内出血,5例因血β人绒毛膜促性腺激素(-hCG)下降停滞而被诊断。7例中2例再次经腹腔镜手术,5例经用氨甲喋呤药物治疗。异位妊娠包块大小、病理有无绒毛组织与是否会发生持续性异位妊娠有关系。结论 对异位妊娠包块越小、 停经时间越短及病理无绒毛患者要高度警惕此症发生。血β-hCG监测对于及时诊断和处理是至关重要的。  相似文献
2.
胃泌素,生长抑素对人胃癌细胞生长的调节作用   总被引:13,自引:0,他引:13  
采用MTT比色法检测细胞增殖情况,用放射法(RIA)测定胰岛素、胰高血糖素和胃泌素含量,氨基己糖含量用Neuhaus法测定,以期观察五肽胃泌素和生长抑素对体外培养的人胃癌细胞HGC803和HGC823生长的调节作用。结果显示,胃泌素对两株胃癌细胞增殖均有促进作用,以1×10^-5mol/L浓度最为明显,并使胃癌细胞氨基己糖含量明显增加(7.58±0.66vs4.2±0.39pg/细胞,P〈0.05  相似文献
3.
The dose-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by whole-body X-ray irradiation (WBI) was studied in male Kunming mice. The inductive doses (D1) were 25, 50, 75, 100 or 200 mGy 6 h before the challenging doses (D2) of 1.0, 1.5 or 2.0 Gy. The changes in the percentages of the thymocyte apoptotic bodies (TAB) and the cells in different phases of cell cycle were measured with flow cytometry. The percentages of TAB decreased, the arrests of G1 and G2 + M phases diminished, and the cells of DNA synthesis of S phase increased when the D1 + D2 groups was compared with the D2 groups. When D1 was 200 mGy, the adaptive response of thymocyte apoptosis and cell cycle progression were no longer induced by low dose radiation (LDR). In addition, the extracellular fluid from the splenocytes were cultured with Con A for 48 h in vitro 24 h after 75 mGy WBI was placed in the murine thymocyte suspension from mice irradiated with 2.0 Gy WBI and co-incubated. The thymocyte apoptosis decreased. Especially, noteworthy was that the percentages of TAB after the incubation for 72 h were significantly lower than those in 2.0 Gy irradiated thymocytes (P < 0.05). These results indicate that when the mice were irradiated with 25-100 mGy (D1, 12.5 mGy/min) 6 h before 1.0-2.0 Gy (D2, 0.287 Gy/min) exposure, an adaptive response of thymocyte apoptosis and cell cycle progression may be induced under the condition of WBI, and LDR (75 mGy) may change the microenvironment of immune cells and decrease the thymocyte apoptosis.  相似文献
4.
人乳腺浸润性导管癌端粒酶活性的检测   总被引:10,自引:0,他引:10  
Wu S  Liu Z  Sun H 《Zhonghua yi xue za zhi》1998,78(7):515-516
目的比较乳腺浸润性导管癌及乳腺良性病变组织端粒酶活性的异同,探讨端粒酶活性在恶性肿瘤诊断中的意义。方法用端粒酶重复扩增法(TRAP)检测了61例乳腺浸润性导管癌及14例乳腺良性病变组织端粒酶活性。结果61例中49例(80%)显示端粒酶活性,端粒酶活性与浸润性导管癌的分级、肿瘤大小、淋巴结转移及肿瘤组织雌激素受体及孕激素受体表达无关。5例乳腺囊腺病无一例端粒酶活性,9例乳腺腺瘤中4例端粒酶弱阳性。结论端粒酶活性见于绝大多数乳腺浸润性导管癌及极少数的乳腺腺病中,该酶活性可能在乳腺癌的发生发展中起重要作用  相似文献
5.
目的 动态观察哮喘动物模型气道嗜酸性细胞凋亡在炎症中的意义和调控机理。研究IL-5和IL-10对凋亡的调节作用。方法 卵白蛋白(OVA)腹腔注射与雾化吸入诱发BALB/c小鼠哮喘发作,在激发后0、8、24、48、96小时及7、14天行BAL,ELISA法测定IL-5、IL-10浓度。CD15、CD49d双色标记嗜酸细胞(EOS),流式细胞仪上检测EOS凋亡率。结果 OVA激发后电镜观察显示肺内有明显炎性细胞浸润和细胞凋亡。哮喘组EOS凋亡水平与对照大体相似,EOS凋亡率与IL-10/IL-5比值密切相关。结论 IL-5和IL-10分别上行和下行调节哮喘气道炎症。气道局部有EOS凋亡现象,并受到IL-5、IL-10相互作用的调节。  相似文献
6.
乳香提取物诱导Jurkat细胞凋亡的实验研究   总被引:8,自引:0,他引:8  
检测不同浓度、不同时间作用了下乳香提取物对人急性T淋巴细胞血病细胞株Jurkat细胞的促凋亡作用。方法:用琼脂糖凝胶电泳技术、透射电子显微镜(TEM)和流式细胞仪(FCM)分别检测Jurkat细胞的DNA梯状带,形态学改变及凋亡峰与药物作用的细胞周期。  相似文献
7.
成都地区中年男性高脂血症患者血脂,载脂蛋白含量研究   总被引:8,自引:1,他引:7  
调查血脂含量,特别是弄清中年男性高脂血症的特征及其变化规律对防治心血管病有着特殊重要的意义。为此,作者对223例中年男性HL患者和年龄,性别相当的349例健康人的体重指数、空腹血清、血脂及apoAⅠ、AⅡ、B100、CⅡ、CⅢ及E的含量进行分析。  相似文献
8.
CONTEXT: Typing of Mycobacterium tuberculosis could provide a more sensitive means of identifying outbreaks than use of conventional surveillance techniques alone. Variants of the New York City W strain of M tuberculosis were identified in New Jersey. OBJECTIVE: To describe the spread of the W family of M tuberculosis strains in New Jersey identified by molecular typing and surveillance data. DESIGN: Population-based cross-sectional study. SETTING AND SUBJECTS: All incident culture-positive tuberculosis cases reported in New Jersey from January 1996 to September 1998, for which the W family was defined by insertion sequence (IS) IS6110 DNA fingerprinting, polymorphic GC-rich repetitive sequence (PGRS) typing, spacer oligotyping (spoligotyping), and variable number tandem repeat (VNTR) analysis. MAIN OUTCOME MEASURE: Identification and characterization of W family clones supplemented by surveillance data. RESULTS: Isolates from 1207 cases were analyzed, of which 68 isolates (6%) belonged to the W family based on IS6110 and spoligotype hybridization patterns. The IS6110 hybridization patterns or fingerprints revealed that43 patients (designated group A) shared a unique banding motif not present in other W family isolates. Strains collected from the remaining 25 patients (designated group B), while related to W, displayed a variety of IS6110 patterns and did not share this motif. The PGRS and VNTR typing confirmed the division of the W family into groups A and B and again showed group A strains to be closely related and group B strains to be more diverse. The demographic characteristics of individuals from groups A and B were specific and defined. Group A patients were more likely than group B patients to be US born (91 % vs 24%, P<.001), black (76% vs 16%, P<.001), human immunodeficiency virus positive (40% vs 0%, P = .007), and residents of urban northeast New Jersey counties (P<.001). Patients with group B strains were primarily non-US born, of Asian descent, and more dispersed throughout New Jersey. No outbreak had been detected using conventional surveillance alone. CONCLUSIONS: The implementation of multiple molecular techniques in conjunction with surveillance data enabled us to identify a previously undetected outbreak in a defined geographical setting. The outbreak isolates comprise members of a distinct branch of the W family phylogenetic lineage. The use of molecular strain typing provides a proactive approach that may be used to initiate, and not just augment, traditional surveillance outbreak investigations.  相似文献
9.
170例乳腺癌标本药物敏感性分析   总被引:7,自引:0,他引:7  
检测了170例原代乳腺癌细胞常用化疗药物的体外敏感性,发现:(1)乳腺癌细胞对5-氟脲嘧啶(5-Fu)、丝裂霉素C(MMC)、顺铂(DDP)及泰素(taxol)的高度敏感率(HSR)明显高于其它受试药物;(2)药物敏感性与乳癌的病理分型和临床分期、以及雌激素受体(ER)和表皮生长因子受体(EGFR)表达状况无明显关系;(3)环孢素A(CsA)能提高部分耐药乳腺癌对阿霉素(ADM)的敏感性。  相似文献
10.
2型糖尿病肾病患者肾小球系膜细胞表型及功能改变   总被引:7,自引:0,他引:7  
Liu Z  Chen Z  Li Y 《Zhonghua yi xue za zhi》2001,81(22):1369-1373
目的研究2型糖尿病肾病系膜细胞表型和功能的改变,进一步探讨糖尿病肾病的发病机理.方法经肾活检从2型糖尿病肾病患者获取肾组织,体外培养系膜细胞.采用流式细胞术、3H-胸腺嘧啶掺入及细胞倍增时间观察细胞表型改变.系膜细胞α-平滑肌肌动蛋白(α-SMA)、层粘连蛋白、纤维连接蛋白的表达用免疫荧光染色及流式细胞仪检测.用2-脱氧-3H-葡萄糖(2-DG)测定细胞葡萄糖摄入.Northern杂交和流式细胞仪检测葡萄糖转运蛋白1(GLUT1)的表达.细胞谷氨酰胺6-磷酸果糖转氨酶(GFAT)的活性采用比色法测定.结果 2型糖尿病肾病来源的系膜细胞较正常对照表现出细胞体积增大、RNA/DNA比值增加并伴细胞增殖加快,细胞骨架蛋白α-SMA和细胞外基质合成增加.糖尿病肾病系膜细胞的葡萄糖摄入率高于对照(1 592 cpm·105 cell-1 与 1 275 cpm·105 cell-1,P<0.05),同时伴GLUT1mRNA及蛋白质表达增加.此外,糖尿病肾病系膜细胞的GFAT活性明显增高.结论 2型糖尿病肾病系膜细胞具有明显的表型与功能改变.此外,糖尿病肾病患者系膜细胞还表现出细胞糖摄入及己糖胺通路活性增加.上述表型及功能改变可能是糖尿病肾病系膜细胞病变形成的基础.  相似文献
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