心房颤动动物模型的制备及应用进展

心房颤动(房颤)是最常见的心律失常,该文介绍常用房颤模型的建立方法,其中快速起搏法最常用,实验动物以犬和兔为主。房颤的疾病模型和基因模型也在逐渐丰富。     

硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响

Objective To investigate the effects of endogenous and exogenous hydrogen sulfide (H2S) on the KATP current in isolated rat ventricular myoeytes. Methods Ventrieular myoeytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATP current was significantly reduced by 200 μmol/L DL-propargylglyeine (PPG, an irreversible inhibitor of the H2S) [(5.3258±0.7556) pA/pF vs. (3.7856±0.4312) pA/pF, P ＜ 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS(a H2S donor, 9.375, 18.75, 37.5,75, 150 μmol/L) in a concentration-dependent manner [(6.6310±0.6092) pA/pF vs. (9.0949±1.0259)pA/pF at 150 μmol/L, P ＜ 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.     

室内外氡浓度与γ辐射水平相关性的探讨

氡是地层岩石和土壤中镭衰变产生的放射性气体,γ外照射主要产生于原生放射性核素~(4‘)K,~(238)U和~(232)Th系。随着人们对氡可致肺癌危害的深入认识,环境中氡水平及对健康的影响已引起人们的关注,为了探索地面室内外空气中氧浓度与γ辐射水平的关系,于1989年3月至1990年6月,对乐山地区地面室内外空气中氡及其子体浓度和γ辐射水平进行了分析测定,现对测定结果作如     

低强度激光照射诱导大鼠胸主动脉金属硫蛋白的生成

目的 :观察低强度激光照射对大鼠胸主动脉金属硫蛋白(ｍｅｔａｌｌｏｔｈｉｏｎｅｉｎ ,ＭＴ)生成的影响 ,以探讨激光照射的细胞保护机理。方法 :选用 3、6、1 2Ｊ ｃｍ2 3个剂量低强度激光对体外孵育的大鼠胸主动脉进行照射。采用竞争性ＲＴ ＰＣＲ法测定胸主动脉内ＭＴ的ｍＲＮＡ水平 ,以1 0 9Ｃｄ牛血红蛋白饱和法测定ＭＴ的含量。结果 :经 3、6、1 2Ｊ ｃｍ2 的激光照射后 ,血管的ＭＴ1ｍＲＮＡ水平分别比对照组增加 1 75 % (Ｐ >0 0 5)、41 8 6 % (Ｐ <0 0 5)和 668 2 % (Ｐ <0 0 1 ) ;ＭＴ2ｍＲＮＡ水平分别比对照组增加1 63 9(Ｐ …     

硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响

Objective To investigate the effects of endogenous and exogenous hydrogen sulfide (H2S) on the KATP current in isolated rat ventricular myoeytes. Methods Ventrieular myoeytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATP current was significantly reduced by 200 μmol/L DL-propargylglyeine (PPG, an irreversible inhibitor of the H2S) [(5.3258±0.7556) pA/pF vs. (3.7856±0.4312) pA/pF, P ＜ 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS(a H2S donor, 9.375, 18.75, 37.5,75, 150 μmol/L) in a concentration-dependent manner [(6.6310±0.6092) pA/pF vs. (9.0949±1.0259)pA/pF at 150 μmol/L, P ＜ 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.     

硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响

Objective To investigate the effects of endogenous and exogenous hydrogen sulfide (H2S) on the KATP current in isolated rat ventricular myoeytes. Methods Ventrieular myoeytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATP current was significantly reduced by 200 μmol/L DL-propargylglyeine (PPG, an irreversible inhibitor of the H2S) [(5.3258±0.7556) pA/pF vs. (3.7856±0.4312) pA/pF, P ＜ 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS(a H2S donor, 9.375, 18.75, 37.5,75, 150 μmol/L) in a concentration-dependent manner [(6.6310±0.6092) pA/pF vs. (9.0949±1.0259)pA/pF at 150 μmol/L, P ＜ 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.     

氧化低密度脂蛋白对人红细胞左旋精氨酸转运的影响

目的观察氧化低密度脂蛋白对健康人红细胞左旋精氨酸（L-arg）转运的影响。方法用低密度脂蛋白和氧化低密度脂蛋白分别孵育正常人红细胞后将细胞分为对照组、低密度脂蛋白组和氧化低密度脂蛋白组,以^3H标记的L-arg（^3H L-arg）来测定红细胞的L-arg转运。结果低密度脂蛋白对红细胞L-arg转运无明显影响（P〉0．05）。氧化低密度脂蛋白抑制红细胞L-arg转运：氧化低密度脂蛋白组氧化低密度脂蛋白25、50、100mg／L3个浓度总摄入的最大转运速率（Vmax）值分别较对照组降低33％、27％、30％（P均〈0.05）,亲和力明显减小（米氏常数值增大,P〈0．05）,均有显著性差异,未见明显的浓度-效应依赖关系;与对照组相比,氧化低密度脂蛋白组氧化低密度脂蛋白25、50、100mg／L3个浓度y＋载体介导的L～arg转运的最大转运速率值明显降低,分别降低43％、48％、45％（P均〈0．05）,均有显著性差异,亲和力明显减小（米氏常数值增大）;与对照组相比,氧化低密度脂蛋白组通过y＋L载体介导的L-arg转运（最大转运速率值及米氏常数值）均无明显改变（P〉0．05）。结论低密度脂蛋白氧化成氧化低密度脂蛋白后抑制红细胞L-arg的跨膜转运,推测氧化低密度脂蛋白是高脂血症患者红细胞L-arg／一氧化氮途径功能紊乱的重要因素。     

新型气体信号分子硫化氢与高血压发病

新型气体信号分子硫化氢广泛参与了高血压发病机制的各个环节,包括调节血管张力、抑制炎性因子、抑制血管重塑和对心肌的负性肌力作用等方面,在高血压的发生、发展中具有重要意义。文章对硫化氢参与高血压发病机制的研究进行综述。     

使用手机增加脑组织糖代谢

一项纳入47名健康人的随机交叉研究发现,接听电话与手机关闭时相比,靠近天线的脑区域糖代谢增加。接听电话时产生的电磁场可能影响脑组织的糖代谢。