改良中性α－糖苷酶与其它精液参数的关系

采用ＷＨＯ１９９２中性α－糖苷酶测定法检测了１８４例男性不育症者精浆，用以探讨中性α－糖苷酶和其它精液参数之间的联系。在精子计数和α－糖苷酶活性（ｍｕ／ｍｌ）（ｒ＝０．４５，Ｐ＜０．０１）、总活性（ｍｕ／Ｔ）（ｒ＝０．３８，Ｐ＜０．０１）之间，精液量和α－糖苷酶活性、总活性之间均有线性正相关；快速前向精子活力和α－糖苷酶之间不相关；ｐＨ和α－糖苷酶之间有抛物线关系。结果显示，在阻塞性无精子症的诊断中，精液参数和α－糖苷酶活性反映附睾阻塞及附睾小管损害的变异程度方面，改良中性α－糖苷酶检测法优于普通总α－糖苷酶检测法。     

免不1号对雄鼠免疫性不育症的作用

目的研究中药免不1号对抗精子抗体引起的免疫性不育症作用方式.方法用精子抗原免疫昆明种雄性小鼠,建立免疫性不育动物模型,同时分别饲喂免不1号和强的松并观察血清精囊液抗精子抗体,精囊液果糖、睾丸附睾病理学、免疫组织化学和妊娠等指标.结果免不1号能明显降低血清抗精子抗体(AsAb)(与模型组比较P＜0.05),且停药后血清、精囊液AsAb反跳幅度小(与西药对照组比较P＜0.01).免不1号能较明显地清除睾丸、附睾免疫复合物的沉积(与模型组、西药组比较),免不1号每曲细精管精子细胞数(±s)(128.1±23.7)显著高于西药组(99.6±18.8)模型组(110.25±21.56)(P＜0.01,P＜0.05),明显提高动物的妊娠,每胎仔数(12.2±2.6)明显高于模型组(9.2±3.3)及西药组(7.8±5.2)(P＜0.05).结论免不1号通过调节全身免疫系统,吸收、清除循环和局部AsAb、免疫复合物,提高精子细胞数,从而提高小鼠的妊娠.     

Traditional Chinese Medicine Compound Mianbu Mix－fang Is Effective in Treating Immunological Infertility：a Female Mice

Objective To study the therapeutic effectivess of Traditional Chinese Medicinecompound mixture Mianbu Fang (Immunological infertility therapy) on immunologi-cal infertility caused by antisperm antibody (AsAb) in female mice.Materials & Methods Forty-two female Kunming mice were evenly divided into 7groups by weight. Group A was control group; Group B was model of infertility.Group C, D and E were fed with normal, half and double dosage of Mianbu Ⅰ respec-tively. Group F and G were fed with Mianbu Ⅱ and prednisone Acetates respectively.Animal model of immunological infertility were set up by injecting mice sperm to theother 36 Kunming female mice except Group A. The AsAb levels in serum, cervicalmucus were measured, the histological and immunohistochemistry changes in ovary andendometrium were observed, and the pregnancy indexes were compared in differentgroups.Results Compared with the infertility model group, the AsAb level in serum andcervical mucus in treatment group was lower. Less immune compounds in ovary andendometrium and atretic follicle of ovary was found in treatment group than in modeland control group. The immune compounds in ovary and endometrium were less in thetreatment group than that in the model and control group.Conclusion By regulating immunological system, Traditional Chinese Medicinecompound mixfang Mianbu Fang lowers AsAb in the circulation system and specialorgans, eliminates immunological compound, repairs tissue impairment and increases pregnancy of female mice.     

不孕妇女抗精子抗体的检测及临床意义

目的：探讨不孕妇女血清和宫颈粘液中抗精子抗体不同类型发生率,相关性及与不孕的关系。方法：用酶联免疫吸附法分别测定每一研究对象的血清和宫颈粘液中的ＡｓＡｂ。结果：不孕组血清,宫颈粘液ＡｓＡｂ总阳性率分别是２５％,３１．８３％,均显著高于生育组。各类抗体阳性率分别是血清中ＩｇＡ型１０．６３％,ＩｇＧ型２２．５％,两者有显著差异;