垂体生长激素腺瘤患者血清miR-212与SIRT1表达及与预后相关性

【摘要】 目的 探讨微小RNA-212（miR-212）、沉默信息调节因子1(SIRT1)在垂体生长激素（GH）腺瘤患者血清中的表达水平及与预后的关系。方法 纳入2016年1月~2019年8月空军军医大学第一附属西京医院收诊的58例垂体GH腺瘤患者设为观察组,选取同期于本院体检的62例健康者设为健康组;采用实时荧光定量PCR（qRT-PCR）法检测所有研究对象血清miR 212、SIRT1、缺氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）表达水平;比较不同预后垂体GH腺瘤患者血清miR-212、SIRT1、HIF-1α、VEGF水平;采用Pearson法分析垂体GH腺瘤患者血清miR-212、SIRT1表达水平与HIF-1α、VEGF相关性及miR-212表达水平与SIRT1的关系;采用受试者特征工作曲线（ROC）评价血清miR-212、SIRT1对垂体GH腺瘤患者预后的诊断价值。结果 观察组患者血清miR-212表达水平明显低于健康组（t=7.434,P＜0.05）,血清SIRT1、HIF-1α、VEGF表达水平明显高于健康组（P＜0.05）;未缓解组垂体GH腺瘤患者血清miR-212表达水平明显低于缓解组（P＜0.05）,血清SIRT1、HIF-1α、VEGF表达水平明显高于缓解组（t=2.536、3.242、2.638,P＜0.05）;垂体GH腺瘤患者血清miR-212表达水平与HIF-1α、VEGF、SIRT1水平均呈负相关（P＜0.05）,血清SIRT1表达水平与HIF-1α、VEGF呈正相关（P＜0.05）;血清miR-212、SIRT1水平对垂体GH腺瘤患者不良预后评估的曲线下面积（AUC）为0.873、0.862,对应截断值分别为0.52、1.94,此时对应灵敏度分别为81.3%、75.0%,特异度分别为88.1%、85.7%;两者联合诊断垂体GH腺瘤的AUC为0.938,其灵敏度、特异度分别为93.8%、81.0%。结论 垂体GH腺瘤血清miR-212表达下调,SIRT1表达上调,两者可能与HIF-1α、VEGF相互作用,进而协同垂体GH腺瘤发生与发展,miR-212、SIRT1有望成为评估垂体GH腺瘤预后的参考指标,两者联合检测可有效提高垂体GH腺瘤预后价值。     

Dialysis Method Alters the Expression of MicroRNA‐33a and Its Target Genes ABCA1, ABCG1 in THP‐1 Macrophages

Atherosclerosis and accompanying cardiovascular disease are the first causes of mortality in patients undergoing maintenance hemodialysis. Anti‐atherosclerotic effects of hemodiafiltration (HDF) have been reported. Our study aimed to investigate the effect of serum derived from a healthy group (n = 23), before and after hemodialysis (HD) therapy (n = 23), and before and after HDF therapy (n = 17) on the expression of microRNA‐33a and its target genes adenosine triphosphate‐binding cassette transporter A1,G1 (ABCA1, ABCG1) in THP‐1 macrophages. Meanwhile, blood lipids and high‐sensitivity C‐reactive protein (hs‐CRP) were measured in these groups. Our data showed that the expression of miRNA‐33a was lower (P < 0.05) and ABCA1 and ABCG1 were higher (P < 0.05) in the healthy group than pre‐HD and pre‐HDF. miR‐33a was significantly decreased (P < 0.05) but ABCA1, ABCG1 was significantly increased (P < 0.05) in post‐HDF compared with pre‐HDF, while these parameters in pre‐ and post‐ HD groups did not show any significant change (P > 0.05). High density lipoprotein cholesterol (HDL‐C) was higher and hs‐CRP was lower in the healthy group than pre‐HD and pre‐HDF groups. Moreover, a significant increase of HDL‐C (P < 0.05) and decrease (P < 0.05) of hs‐CRP was shown in post‐HDF compared with pre‐HDF, but HD appeared to have no significant change in these subjects. HDF therapy can downregulate miR‐33a expression, and then result in ABCA1, ABCG1 upregulation and an increase in circulating HDL‐C, leading to a possible anti‐atherosclerosis effect to some extent.     

MicroRNA和DNA甲基化在肺癌诊断中的研究进展

小分子核糖核酸（MicroRNA）作为肺癌早期的一种新的生物标志物,为肿癌的诊断提供了一种新的方法。MicroRNA在肿瘤发生发展过程中发挥癌基因和抑癌基因的作用。MicroRNA与肺癌增殖、侵袭、治疗、预后和复发有着密切关系。DNA甲基化是目前最主要的一种表观遗传修饰形式,抑癌基因的高甲基化使DNA染色质构象发生改变导致转录失活,引起肿瘤发生。DNA甲基化和MicroRNA在功能上相互调控,对肺癌的发生、发展同样重要。     

微小RNA与牙周炎

近十年,一类非编码蛋白的小RNA分子——微小RNA (miRNAs)证实参与免疫细胞发育以及多种疾病如肿瘤、炎症等生理和病理过程.而牙周炎作为危害人类口腔健康的常见慢性炎症,其发病机制的研究尚有不确切之处,也未能找到十分有效的治疗方法.近年,学者开始对牙周炎及miRNAs两者间的关系开展研究,以探索miRNAs参与牙周炎的发病机制.本文根据miRNAs与牙周炎研究的最新进展,就其在牙周组织及牙槽骨组织中异常表达及可能机制两方面作一综述.     

Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer

AIM: To investigate whether selected single nucleotide polymorphisms (SNPs) in miR-196a2, miR-27a and miR-146a genes are associated with sporadic colorectal cancer (CRC).METHODS: In order to investigate the effect of these SNPs in CRC, we performed a case-control study of 197 cases of sporadic CRC and 212 cancer-free controls originating from the Central-European Caucasian population using TaqMan Real-Time polymerase chain reaction and allelic discrimination analysis.RESULTS: The genotype and allele frequencies of SNPs were compared between the cases and the controls. None of the performed analysis showed any statistically significant results.CONCLUSION: Our data suggest a lack of association between rs11614913, rs895819 and rs2910164 and colorectal cancer risk in the Central-European Caucasian population, a population with an extremely high incidence of sporadic colorectal cancer.     

微小RNA在心肌重构中的作用

微小RNAs（miRNAs）是生物体中内源性的调控因子,它通过抑制相应mRNAs的翻译或降解特异mRNAs来调节特异基因的表达。新近研究发现miRNAs能调控心血管疾病的发生发展,如心肌肥厚、心脏纤维化、心律失常、心力衰竭等,参与了心肌的机械重构和电重构过程。随着对它的深入研究,发现人和动物心脏受损时血浆中某些miRNAs特异升高。以上发现使miRNAs有可能成为诊断心肌重构的生物标志物和治疗靶点。本文重点阐述miRNAs在心肌机械重构和电重构中的调控作用及其临床意义。     

MicroRNA在支气管哮喘中作用的研究进展

微小RNA(microRNA)是一类内源性的短链非编码小RNA,能与特定的信使RNA靶向结合,在转录后水平调控基因表达.MicroRNA可在人体许多生理和病理过程的多个环节发挥其调控作用.近年来的一些研究表明microRNA与支气管哮喘(简称哮喘)等气道炎症性疾病密切相关,下面就microRNA在哮喘中作用的研究进展作一综述,以使我们加深对哮喘发病机制的认识,更好地探索新的有效的治疗策略.