钙黏蛋白1在少弱精子症精子中的表达

目的:通过检测钙黏蛋白1(CIB1)在少弱精子症患者精子中的表达,探讨其与少弱精子症发病机制的关系。方法:收集25例少弱精子症患者精液标本,其中少弱精子组13例和弱精子组12例,另同期收集正常对照组19例。分别采用半定量PCR(RT-PCR)及荧光定量PCR(qRT-PCR)方法和Western印迹检测,并比较各组精子中CIB1和周期蛋白依赖性激酶1(CDK1)的mRNA及蛋白表达量。结果:1RT-PCR和qRT-PCR检测表明:在少弱精子组、弱精子组和正常对照组的CIB1mRNA的相对表达量均呈上升趋势,而CDK1 mRNA的相对表达量均呈下降趋势,CIB1 mRNA与CDK1 mRNA在3组间两两比较,除qRT-PCR检测中少弱精子组与弱精子组差异无统计学意义(P0.05),其余组间比较差异均有统计学意义(P0.01)。2CIB1蛋白的相对表达量在少弱精子组、弱精子组和正常对照组中呈上升趋势,3组间两两比较,差异均有统计学意义(P0.05;P0.01);而CDK1蛋白的相对表达量呈下降趋势,其中少弱精子组与正常对照组比较,差异有统计学意义(P0.05)。结论:CIB1可能通过CDK1通路参与少弱精子症的发病机制。     

Intracytoplasmic Sperm Injection Fertilization Rate Does Not Depend on the Proportion of Round-headed Sperm,Small-acrosomal Sperm,or Morphologically Normal Sperm in Patients with Partial Globozoospermia

Background:

Generally, intracytoplasmic sperm injection (ICSI) may be the preferable method to treat partial globozoospermia, but whether there exist some correlations between ICSI fertilization rate and the proportion of round-headed sperm or morphologically normal sperm remains open. This study was to explore the correlation between ICSI fertilization rate and the sperm morphology in patients with partial globozoospermia.

Methods:

Thirty-four patients diagnosed with partial globozoospermia accepted the following assisted fertilization treatments – 2 cases accepted in-vitro fertilization (IVF) alone, 26 cases accepted ICSI alone, and 6 accepted split IVF/ICSI. Detailed morphological characteristics were described using Diff-Quik rapid staining. Sixty cases accepting IVF or ICSI treatment in our reproductive center were considered as the control group after being matched by relevant criteria. Fertilization rate, embryo quality, embryo implantation rate and clinical pregnancy rate were calculated.

Results:

Besides very high proportion of round-headed sperm, partial globozoospermia also showed very high proportion of small-acrosomal sperm and very low proportion of morphologically normal sperm. Fertilization rate of IVF (IVF alone plus split IVF) was very low in partial globozoospermia (25.4% ± 17.4%), but ICSI (ICSI alone plus split ICSI) achieved satisfying fertilization rate compared with the control group (66.2% ± 22.5% vs. 68.8% ± 29.4%, P > 0.05). In patients with partial globozoospermia, there were no correlations between ICSI fertilization rate and the proportion of round-headed sperm, small-acrosomal sperm, or morphologically normal sperm.

Conclusions:

There was high proportion of small-acrosomal sperm in partial globozoospermia. For patients with partial globozoospermia, ICSI is more preferable than IVF. ICSI fertilization rate does not depend on the proportion of round-headed sperm, small-acrosomal sperm, or morphologically normal sperm.     

何首乌饮对衰老大鼠精子DNA完整性的影响

目的：：通过观察大鼠精子DNA完整性的变化,探讨何首乌饮对衰老大鼠精子质量的影响。方法：雄性Wistar大鼠随机分为青年对照组、自然衰老组、何首乌丸组、何首乌饮1组和2组。采用精子DNA吖啶橙荧光检测试剂盒检测精子DNA完整性,荧光显微镜下观察,头部发绿色荧光的为DNA正常精子,发红色或黄色荧光为DNA异常精子。结果：自然衰老组大鼠头部发绿色荧光精子数量明显减少,发红色或黄色荧光的DNA异常精子显著增多,与青年对照组比较差异具有统计学意义(P＜0.01）;何首乌饮和何首乌丸干预组头部发绿色荧光的精子比自然衰老组大鼠显著增多(P＜0.01,P＜0.05）,且用药60d何首乌饮组(1组）头部发绿色荧光的精子明显多于其它两个用药组(P＜0.01）。结论：何首乌饮能明显改善自然衰老大鼠精子DNA的完整性,提高精子质量。     

精子DNA碎片率与体外受精实验室结局的关系

目的初步探讨精子DNA碎片率、精子核蛋白不成熟度与体外受精实验室结局的关系。方法对2013年5~7月在我院行IVF治疗的男性患者行精液常规、精子DNA碎片率及精子核蛋白不成熟度检查,利用spearman相关分析探讨它们与IVF受精率、优胚率的关系。结果精子DNA碎片碎与男方年龄正相关,与前向运动精子百分率、优胚率负相关,与IVF受精率无相关。精子核蛋白不成熟度与精子浓度、正常精子百分率负相关,与IVF受精率、优胚率无显著相关。结论精子DNA碎片率检测对预测IVF优胚率有一定的临床意义。     

多功能超高倍显微分析仪在不育症精液分析中的应用

目的探讨运用多功能超高倍显微分析仪直接湿片观察精子形态的应用价值。方法支原体感染不育症患者156份精液标本,用改良巴氏染色法,1000倍油镜观察精子形态,同时运用多功能超高倍显微镜直接湿片观察精子形态。结果两种方法对精子形态的评估经统计学分析,正常形态率和各种类型缺陷率差异均无统计学意义（P〉0．05）．结论运用多功能超高倍显微分析仪直接观察精子形态是一种简单、快速、有效的方法,值得推广。     

不育厨师精液质量参数及精子DNA完整性分析

目的探讨不育厨师精液常规质量参数和精子畸形率、精子DNA碎片率等指标。方法对生殖门诊52名不育厨师（观察组）和49名育前体检者（对照）行精液常规和DNA碎片率等检测。结果观察组精液液化异常率、精子畸形率、DNA碎片率都显著高于对照组;精子浓度、前向运动精子比率显著低于对照组。结论厨师职业暴露会影响精液质量,导致男性不育,应加强保护和防范。     

吸烟对男性精子形态 精子DNA完整率和精子顶体完整率的研究

目的：研究吸烟对男性精子形态、精子DNA完整率[用精子DNA断裂指数（ DFI）表示]及顶体完整率的影响。方法回顾性分析来该中心就诊的1500例男性患者（1300例吸烟者及200例未吸烟者）,按每天吸烟量分为A1组500例,＞20支／d;A2组600例,10-20支／d;A3组200例,1-9支／d。 B组200例,0支／d,作为对照组。结果精子正常形态随着每天吸烟量的增加而下降,各组间两两比较差异有统计学意义（ P＜0．05）。精子DNA完整率随着吸烟量的上升而降低（ DFI升高）,各组间两两比较差异有统计学意义（ P＜0．05）。精子顶体完整率随吸烟量上升而下降,各组间两两比较差异有统计学意义（ P＜0．05）。结论随着每天吸烟量增加精子正常形态、精子DNA完整率及精子顶体完整率均降低,不利于男性生殖健康。     

Sperm DNA damage in patients with chronic viral C hepatitis

Introduction

The aim of this study was to evaluate the conventional and biofunctional parameters of sperm in young infertile patients with Hepatitis C (HCV) infection.

Methods

Forty HCV patients with primary infertility, aged 27 to 42 years (mean 36.4 years) and twenty HCV patients with secondary infertility aged 28 to 45 years (mean 35.0 ± 2.8 years), underwent hormonal and sperm analysis in addition to the determination of reactive oxygen species (ROS) concentrations in the sperm and flow-cytometric evaluation. The following biofunctional sperm parameters were evaluated by flow cytometry: DNA fragmentation, mitochondrial membrane potential, chromatin condensation, and the rate of early apoptosis.

Results

Overall, patients with HCV showed significantly worse median values of conventional and biofunctional sperm parameters than control subjects, including sperm density (31.7 vs. 80.4 million/ml), forward motility (9.4 vs. 25%), normal forms (15.4 vs. 24.8%), DNA fragmentation (6.6 vs. 2.2%), low MMP (45.5 vs. 8%), an early apoptosis rate (5 vs. 2.7%), and abnormal chromatin (18.9 vs. 13.9%). Finally, HCV patients had significantly higher basal (250 vs. 75 × 10³/cpm) and stimulated (550 vs. 120 × 10³/cpm) ROS levels in semen compared to control subjects. None of the examined parameters (sperm, hormonal, biofunctional and assessment of oxidative status in the semen) was significantly different between HCV patients with primary and secondary infertilities.

Discussion

These results confirm that HCV infection has a negative impact on sperm parameters. The overlap of the results observed in the two groups of HCV patients supports the hypothesis that HCV infection may cause to alterations in sperm parameters.     

精子尾部卷曲试验与精子低渗肿胀试验的共性与特性

探讨了精子尾部卷曲试验与精子低渗肿胀试验的关系。两种实验原理相同，实验条件（渗量、离子浓度）不同，实验结果存在一定差异。对９５例生育和不育男性精液检查结果表明：精子卷尾率与精子尾总肿胀率呈高度正相关（ｒ＝０．９１２）；精子卷尾率与精子低渗肿胀试验ｇ型精子率比较呈极显著差异（Ｐ＜０．００１）；精子卷尾率、精子低渗总肿胀率与精子活率呈高度正相关（ｒ分别为０．９６３和０．９３２）；生育组与不育组比较精子卷尾率、精子总肿胀率及ｇ型精子率均呈显著性差异（Ｐ＜０．０１）。采用精子卷尾率结合低渗肿胀试验ｇ型精子率综合评估精子的活动功能对男性不育症的实验诊断具有良好的应用价值。