Sodium thiosulfate attenuates high phosphorous induced vascular calcification in rats with 5/6 nephrectomy

Objective To observe the expression of Klotho and Na+/Pi cotransporter in high phosphorous-induced rats with 5/6 nephrectomy and its relationship with vascular calcification, as well as to investigate the effect of early intervention by sodium thiosulfate (STS) on the progression of vascular calcification. Methods Either 5/6 nephrectomy (n=21) or sham operation (n=14) was conducted on 35 Sprague Dawley rats, who were then fed with high phosphorus (HP) diet or normal phosphorus (NP) diet for 16 weeks respectively. The rats were divided into 5 groups as follows: (1) remnant kidney rats receiving HP diet (NHP, n=7), (2) remnant kidney rats receiving NP diet (NNP, n=7), (3) sham operation rats receiving NP diet (SNP, n=7), (4) sham operation rats receiving HP diet (SHP, n=7), (5) remnant kidney rats receiving HP diet with STS (THP, n=7). The treatment group was given STS intraperitoneally three times a week for 16 weeks. At the end of the 16th week, rats tail artery blood pressures were tested, serum creatinine (Scr), calcium (Ca2+), phosphorus (P3+), FGF23, iPTH and urine protein were examined. Throacic aorta and kidney were then removed. Vascular calcification was confirmed by Von kossa staining. Klotho and Pit-1 expression in aortas were determined by immunohistochemistry. Renal lesion was determined by PASM-Masson staining. Renal Klotho and NaPi-2a mRNA were determined by real time RT-PCR. Results After 16 weeks, Scr, P3+, FGF23, iPTH, uric protein and blood pressure were significantly higher in NHP than those in SNP rats (all P＜0.05). PASM-Masson staining revealed typical renal pathology of chronic renal failure in NHP group. With the treatment of STS, THP rats showed significant decrease in Scr, P3+, FGF23, iPTH, uric protein and blood pressure by comparison with NHP group (all P＜0.05). Significant vascular calcification was found in NHP group while NNP and SHP group occasionally had vascular calcification; THP group had marked alleviation of vascular calcification. The aorta and renal expression of Klotho decreased remarkably while expression of Pit-1 and NaPi-2a increased significantly in NHP compared with SNP group (all P＜0.05). Accordingly, the aorta and renal expression of Klotho increased and Pit-1 and NaPi-2a decreased significantly in THP compared with NHP group (P＜0.05). Conclusions The early intervention of sodium thiosulfate might regulate Klotho and Na+/Pi cotransporter expression in both aorth and kidney, decreasing serum phosphate, delaying progression of vascular calcification and improving renal function.     

金欣口服液含药血清对呼吸道合胞病毒黏附、膜融合影响的实验研究

目的:研究金欣口服液含药血清对呼吸道合胞病毒(RSV)黏附、膜融合的影响。方法:在病毒和细胞接触期间采用转换温度方法观察金欣口服液含药血清对RSV黏附、侵入的影响。结果:温度转换前(黏附阶段)加入金欣口服液含药血清,实验组和对照组OD值无差异;温度转换后(侵入阶段)加入金欣口服液含药血清组OD值明显升高,与对照组比有显著性差异。结论:金欣口服液含药血清的抗病毒作用在呼吸道合胞病毒黏附后的膜融合及侵入阶段。     

长甲牌百消丹辐射灭菌剂量设定的研究

采用活菌计数法和D_(10)值测定对长甲牌百消丹辐射灭菌剂量进行设定。结果,3批长甲牌百消丹初始染菌数平均值分别为2620 cfu/g、15630 cfu/g和4900 cfu/g,3批平均总菌数为7717 cfu/g,污染菌数范围为1700～28000 cfu/g。测得D_(10)值为1.75kGy,计算出最低灭菌剂量为17.70kGy。结论,~(60)钴辐照对长甲牌百消丹辐射灭菌剂量为17.70 kGy,确定灭菌保证水平为10~(-3)。     

金实教授运用虫类药治疗类风湿关节炎经验

金实教授认为类风湿关节炎的病理因素紧扣风、寒、湿、热、痰、瘀六端,病理性质总属本虚标实,本虚主要为肝肾不足,气血亏虚,标实以风寒湿邪为主。金实教授认为本病病程较长,病久入络,易形成久痹、顽痹,而虫类药具有很好的搜剔病邪、剔络和络、通络止痛的作用,对类风湿关节炎有很好的治疗效果。现对金教授运用虫类药治疗类风湿关节炎进行经验汇总、分析。     

从脾论治甲状腺功能异常

甲状腺功能异常的发病率逐年上升,现已成为危害人类生命健康的重要卫生问题。基于甲状腺与脾脏在生理上的关联及甲状腺功能异常不同类型的疾病特点,探讨从脾论治甲状腺功能异常。明确甲状腺功能亢进症与甲状腺功能减退症发病的基本病机分别为脾阴不足和脾阳亏虚,并分别给予益气养阴、益气温阳两种基本治法。甲状腺功能亢进症治用甘淡平和及甘酸微寒之药,甲状腺功能减退症治用甘温扶正及辛温开散之药。     

针刺联合温胆汤治疗痰瘀互结型稳定型心绞痛的疗效观察

目的观察针刺联合温胆汤加减治疗痰瘀互结型稳定型心绞痛的临床疗效。方法将符合纳入标准的60例痰瘀互结型稳定型心绞痛患者按照随机数字表法分为对照组和治疗组,每组30例。对照组给予西医常规治疗,治疗组在对照组治疗基础上加以针刺和温胆汤加减治疗。治疗4周后,比较两组安全性指标(血肝/肾功能指标)、中医证候评分、中医证候疗效、血脂指标和相关临床指标(心绞痛发作频次、发作持续时间和硝酸甘油用量)。结果两组治疗后安全性指标(血肝/肾功能指标)与治疗前比较差异无统计学意义(P>0.05),治疗后组间比较差异无统计学意义(P>0.05)。两组治疗后中医证候评分均较治疗前显著降低(P<0.05),治疗组治疗后中医证候评分明显低于对照组(P<0.05)。治疗后,治疗组和对照组中医证候疗效总有效率分别为90.0%和70.0%,两组比较差异有统计学意义(P<0.05)。治疗组治疗后的血脂指标均优于对照组,差异均有统计学意义(P<0.05)。治疗后,治疗组心绞痛发作频次、发作持续时间及硝酸甘油用量均优于对照组,组间差异均具有统计学意义(P<0.05)。结论在西药治疗基础上予针刺联合温胆汤加减治疗可改善痰瘀互结型稳定型心绞痛的中医证候,提高中医证候疗效,改善血脂,减少心绞痛发作频次、发作持续时间和硝酸甘油用量,且无肝肾损伤,安全性高。     

金喉健超声雾化剂联合利咽饮治疗小儿急慢性喉炎的效果评价

目的 探讨金喉健超声雾化剂与利咽饮治疗小儿急慢性喉炎的临床效果.方法 选取2013年1月～2014年2月入本院就诊的400例急慢性喉炎患儿,随机分为观察组和对照组,各200例,观察组采用金喉健超声雾化剂联合利咽饮治疗,对照组采用单一金喉健超声雾化剂治疗,比较两组的治疗效果及患者满意度.结果 观察组患儿治疗总有效率优于对照组（98.5％vs 77.0％）,差异有统计学意义（P＜0.05）;观察组患儿满意度显著高于对照组（98.0％vs 63.0％）,差异有统计学意义（P＜0.05）.结论 金喉健超声雾化剂联合利咽饮治疗小儿急慢性喉炎效果显著,优于单一金喉健超声雾化剂治疗,患者满意度较高,可明显减轻患儿的痛苦,值得临床广泛应用.     

从科场魁元到学界泰斗——张謇兴教生涯考察

张謇是中国近代从科举营垒中叛杀出来的伟大教育家。他经过30年艰辛拼搏登上了科举制度的峰巅,这正好为他从教育的功能与地位、培养目标、教育方针、课程设置、教学方法、实业与教育的关系、教师与师范的地位、学校管理、教育发展规律等方面阐发新的教育思想,并最终创立新的教育体系准备了条件。而他之所以能从旧教育的科场魁元转变为新教育的学界泰斗,是因为他勇于并善于顺应时代潮流、实业先行、推陈出新、洋为中用和循序渐进的结果。考察张謇25年的兴教生涯,可以为今天更好地推进社会主义现代化的文教事业提供有益的借鉴。     

丹红注射液配合苯溴马隆片治疗痛风实例

通过丹红注射液配合苯溴马隆片治疗痛风实例,说明中西医结合治疗痛风,具有治疗效果良好、无反复且副作用少的优势。