Galangin suppresses H2O2‐induced aging in human dermal fibroblasts

Human skin aging is a progressive process that includes intrinsic aging and extrinsic photodamage, both of which can cause an accumulation of reactive oxygen species (ROS), resulting in dermal fibrosis dysfunction and wrinkle formation. Galangin is a flavonoid that exhibits anti‐inflammatory and antioxidative potential. Previous studies have reported that galangin has antioxidative activity against ROS‐mediated stress. The aim of the present study is to determine the antiaging effects of galangin on dermal fibroblasts exposed to H₂O₂. In this study, we established a hydrogen peroxide‐induced inflammation and aging model using human HS68 dermal fibroblasts. Stimulation of fibroblasts with H₂O₂ is associated with skin aging and increased expression of inflammation‐related proteins, along with downregulation of collagen I/III formation and expression of antioxidative proteins. Galangin effectively reduced NF‐κB activation, the expression of inflammation‐related proteins and cell aging. Galangin also reversed H₂O₂‐activated cell senescence in HS68 cells. Our results reveal that galangin protects human dermal fibroblasts by inhibiting NF‐κB activation, decreases the expression of inflammatory factors and upregulates IGF1R/Akt‐related proteins, indicating that galangin may be a potential candidate for developing natural antiaging products that protect skin from damage caused by ROS.     

高良姜素抑制乳腺癌转移作用机制研究

目的探究高良姜素对MDA-MB-231乳腺癌细胞迁移及侵袭的影响及其作用机制。方法采用MTT法观察不同浓度高良姜素对乳腺癌细胞MDA-MB-231增殖的影响;划痕愈合实验和Transwell小室迁移实验考察高良姜素对MDA-MB-231水平迁移能力和垂直迁移能力的影响;Transwell小室侵袭实验分析高良姜素对MDA-MB-231细胞侵袭能力的影响;采用蛋白免疫印迹(Western blotting)法考察高良姜素对MDA-MB-231细胞PI3K、Akt、MMP-2、MMP-9蛋白表达的影响,免疫荧光染色实验验证高良姜素对MDA-MB-231细胞PI3K、Akt、MMP-2、MMP-9蛋白表达的影响;Western blotting法和免疫荧光染色实验考察高良姜素对核转录因子(NF-κB)p65磷酸化表达和入核的影响;制备乳腺癌MDA-MB-231细胞株裸鼠移植瘤模型,高良姜素200μg/kg ig给药15 d,检测肿瘤体积和质量,计算抑瘤率,Western blotting分析肿瘤组织PI3K、AKT、MMP-2、MMP-9表达及NF-κB p65磷酸化表达情况。结果高良姜素体外浓度为50、100、200μmol/L时可显著抑制MDA-MB-231细胞的增殖;高良姜素能剂量依赖性抑制PI3K、Akt、MMP-2、MMP-9蛋白表达,降低NF-κB p65磷酸化表达和入核;200μg/kg高良姜素能抑制裸鼠乳腺癌的生长,抑瘤率为43.66%;高良姜素能抑制瘤组织中PI3K、Akt、MMP-2、MMP-9表达以及NF-κB p65磷酸化表达。结论高良姜素在体内对MDA-MB-231生长有明显的抑制作用,在体外能抑制MDA-MB-231细胞增殖、迁移、侵袭,抑制相关蛋白PI3K、Akt和MMP-2、MMP-9蛋白表达,抑制转录因子NF-κB磷酸化表达及入核,阻断其激活。     

不同地区蜂胶原料药的质量评价

目的:建立不同产地蜂胶的质量评价方法。方法:采用薄层色谱法,以白杨素和高良姜素为对照品,甲苯-乙酸乙酯-甲酸(10∶4∶3)为展开剂,进行定性鉴别研究;采用高效液相色谱法,色谱柱Dikma Technologies(250 mm×4.6mm,5μm);流动相甲醇-0.15%磷酸水溶液(62∶38);流速1.0 mL·min-1;柱温25℃;检测波长268 nm。结果:28个产地蜂胶原料药中白杨素和高良姜素的含量均符合《中国药典》(2010年版)的规定的仅有6个地区,大部分地区蜂胶原料药中高良姜素含量符合标准,而白杨素含量不达标。结论:不同产地蜂胶原料药中白杨素和高良姜素的含量不同,建立的定性、定量方法准确可靠,专属性强,适合不同产地蜂胶原料药的质量控制。     

南药植物高良姜内生真菌群体多样性及其组织分布

采用组织PCR-末端限制性片段长度多态性分析（T-RFLP）免培养技术,从南药植物高良姜道地产地植株根、根茎、茎、叶4个不同组织总共检测到28个不同的末端限制性片段（T-RFs）,说明高良姜内生真菌群体至少组成28个不同的真菌种;不同组织其T-RFLP图谱、T-RFs数目、优势T-RFs组成不同,内生真菌群体Shannon多样性指数和Shannon均匀度指数也随组织而异;采用水蒸气蒸馏法、甲醇抽提-高效液相色谱法测定高良姜主要活性成分挥发油、高良姜素含量,结果显示,该2类活性组分在其根、根茎、茎、叶4个不同组织均有积累,且均以根茎最高;相关性分析显示2类所测活性成分与该宿主内生真菌群体Shannon多样性指数及其均匀度呈极显著的负相关,与325 bp对应于拟盘多毛孢菌属Pestalotiopsis的优势T-RFs呈极显著的正相关。     

生长年限及环境因素对高良姜中高良姜素动态积累的影响

目的： 对采自广东、海南等地区的53批次代表性样品中高良姜素的含量进行测定,探讨生长年限及环境因素对高良姜中高良姜素动态积累的影响。 方法： 采用高效液相色谱法测定,ODS-C₁₈色谱柱（4.6 mm×250 mm,5 μm）,流动相甲醇-0.2%磷酸（55：45）,流速1 mL·min^-1,柱温30℃,检测波长266 nm。 结果： 海南产样品中含量明显高于广东产样品;4年生药材中高良姜素的含量明显高于2,3,5年生样品;在4年生药材中夏末秋初采收样品中高良姜素的含量明显高于初春采收的样品。 结论： 海南栽培高良姜优于广东栽培品,4年生长期为高良姜最佳栽培周期,采收时间应在夏末秋初。     

6种黄酮类化合物拮抗肝细胞毒性作用的比较

目的观察芹菜素、木犀草素、白杨素、高良姜素、山奈酚、黄芩素6种结构相近的黄酮类化合物对肝毒性物质诱导的人正常肝细胞株L-02细胞及小鼠原代肝细胞损伤的保护作用,并对其保护肝细胞损伤活性的强弱进行比较及结构分析。方法培养的L-02细胞及小鼠原代肝细胞分别给予10mmol·L~(-1)四氯化碳(CCl_4)、500μmol·L~(-1)牛磺酸脱氧胆酸(TDCA)、2μmol·L~(-1)川楝素(toosendanin,TSN)和25μmol·L~(-1)千里光碱(senecionine,SENE)诱导细胞凋亡。6种黄酮类化合物(100μmol·L~(-1))分别与细胞预孵15min后,加入肝毒性物质,48h后MTT法检测细胞存活率。结果与空白对照组相比,4种肝毒性药物都能明显降低肝细胞的存活率(P<0.01)。黄酮类化合物处理后,黄芩素和山奈酚能不同程度地提高肝细胞损伤模型组的细胞存活率(P<0.01)。其他4种黄酮类化合物对肝细胞存活率无显著作用。结论黄芩素和山奈酚具有抑制肝毒性物质诱导的L-02细胞和小鼠原代肝细胞毒性的作用,有潜在的抗氧化保肝活性,而芹菜素、木犀草素、白杨素、高良姜素无明显的拮抗肝毒性作用。     

高良姜与香附配伍的不同提取方法的研究

目的:通过测定高良姜与香附配伍的不同提取方法中高良姜素的含量,确定高良姜与香附配伍的提取方法。方法:采用HPLC法,以乙腈-0.5%磷酸(42:58)为流动相,检测波长:266nm,流速:1.0ml·min-1;结果:高良姜与香附的提取方法为乙醇合并提取时,高良姜素的含量高于其他提取方法。结论:高良姜与香附的提取方法为乙醇合并提取时,有助于高良姜中有效成分高良姜素的溶出。     

Effect of galangin on hypoxia-inducible factor-1α-mediated epithelial mesenchymal transformation in gastric cancer cells北大核心CSCD

Aim To investigate the effect of galangin(GLA) on gastric cancer in hypoxic microenvironment. Methods The gastric cancer SGC-7901 cell line was induced with CoCl2 to mimic the hypoxic tumor microenvironment in vitro. Cell viability assay was performed using CCK- 8 reagents. Invasive Ability of Cancer Cells was Analyzed by Transwell Assay. And the expression levels of relevant genes we detecting and rt-QPcr. Results the study showed that low, Medium and High Concentrations of GLA (50, 100 and 150 μmol ·L-1)presented a dose-dependent inhibition of CoCl2 -induced gastric cancer SGC-7901 cell viability. This concentration gradient also showed significant inhibition on cell invasion after CoCl2 intervention(P<0.01). In-depth study revealed that the CoCl2 -induced increased HIF-1α protein expression was inhibited with increasing GLA concentration, while HIF-1α mRNA expression was not affected. Meanwhile, Snail and Vimentin expression of epithelial mesenchy bad transition-related genes significantly increased with increasing GLA dose(P<0.05), whereas E-cadherin expression significantly decreased(P<0.01). Conclusions GLA inhibits HIF-1α expression and epithelial mesenchymal transition under tumor hypoxia, thereby s uppressing gastric cancer development. © 2023 Publication Centre of Anhui Medical University. All rights reserved.