SPSS正交设计提取林蛙油多糖

目的研究中性蛋白酶水解提取林蛙油多糖的最佳工艺参数。方法以料水比、中性蛋白酶浓度、水解时间和水解温度为考察因素,提取液中多糖含量为指标,通过SPSS软件设计L9(34)正交试验,并用SPSS进行数据处理。结果通过正交实验,得出中性蛋白酶提取林蛙油多糖工艺条件为料水比1∶100、加酶量为5%、水解时间1h、水解温度50℃。结论运用SPSS软件设计L9(34)正交表并进行数据处理,大大方便了实验结果处理,使实验方便科学,得出中性蛋白酶水解提取林蛙油多糖的工艺。     

现况调查列联表资料的SPSS分析

现况调查是流行病学的基础性研究方法，SPSS是应用广泛的国际权威统计分析软件。本文结合现况调查列联表资料实例，运用SPSS13．0软件包进行统计分析，对操作过程和结果解释予以详细说明，供广大医学科研工作者在遇到现况调查资料分析时参考。     

新农合绩效综合评价Excel模板与SPSS程序的研制

[目的]研制新型农村合作医疗（NCMS）绩效综合评价Excel速算活模板与SPSS程序,供县级评分和全省各县市综合评分排序。[方法]按照全国大连培训班定下的10个指标及其方向、权重和参考值,用＂指数法＂标化,研制县级用的Excel速算活模板和SPSS速算程序。[结果]在完整录入14个原始指标数据库的前提下,研制成功的Excel速算模板可马上算得县级评价总分;SPSS速算程序可在几分钟内算得全省各县市的排序结果。[结论]研制成功的新农合绩效Excel速算活模板和SPSS速算程序直观实用,在国内有很大的推广应用价值。     

Synthesis and application of peptide immunogens related to the sperm tail protein tpx‐1, a member of the CRISP superfamily of proteins

Abstract: The synthesis of peptides containing 0, 1 and 2 cysteine residues related to the human sperm tail protein, tpx‐1, is described. These synthetic peptides, following conjugation to keyhole limpet hemocyanin modified with maleimidobenzoic acid N‐hydroxysuccinimide ester, were used as immunogens to generate polyclonal antibodies in female New Zealand white rabbits. The binding characteristics of the derived antipeptide sera were evaluated using indirect and competitive ELISA procedures. Western immunoblot experiments also confirmed that these synthetic peptide immunogens are able to generate high‐titer polyclonal antibodies capable of cross‐reacting with the mature tpx‐1 protein present in crude rat sperm tail/testis preparations as well as in outer dense fiber preparations. Consequently, these synthetic peptides represent promising candidates for investigations into the role of tpx‐1 in the immunoregulation of sperm function in the rat and other mammalian models, with the derived antisera also providing an avenue to explore possible sites of expression of tpx‐1 proteins in other tissues.     

基于SPSS的生物统计数据的2D3D作图

目的结合生物统计学的软件SPSS 11.0,进行数据处理和图形结果输出,并对SPSS 11.0图表输出方式加以改进,结合MATLAB进行模拟演示,以得到更加形象并具有动态趋势的统计图。方法结合NCPP和CPP对小鼠脾脏激活作用的比较的数据,对其进行独立样本t检验,然后利用MATLAB编程进行演示。结论实现SPSS 11.0软件与MATLAB编程的相互结合,得到很形象的图形结果。     

硕士生医学统计学实验课改革初探

医学统计学是医学硕士生的一门必修课,是医学科研必不可少的工具,然而硕士生的统计应用能力与教学目标期望值有较大差距。该文分析了当前实验课教学的现状与不足,深入思考了当前医学统计学教学改革的方向,提出了PBL与统计软件SPSS紧密结合的教学模式,并详细阐述了实施及考核方案。该教学模式以解决医学实际问题为出发点,着重培养学生正确利用统计理论和统计分析软件为科研服务的能力。该模式将极大地激发学生的学习热情,有助于形成系统的统计知识体系和科研思路以及增强医学硕士生的统计实践能力。     

基于SPSS的高校学生对教师教学评价的定量分析

通过对我校经管学院学生对教师教学评价的数据用SPSS进行分析,得到了除总分排名外的更为细致的描述性的结果,同时进行了方差分析、因子分析、聚类分析,认为需要重视学生评价的结果,但是要做到对教师公平、公正、客观、人性化的评价需要做更多的工作。     

Kendall协调系数W检验及其SPSS实现

目的检验双因素设计数据是否具有一致性。方法采用非参数Kendall协调系数W检验。结果应用SPSS实现Kendall协调系数W检验。结论该检验方法可用于双因素设计资料,在医学科研中具有应用意义。     

《SPSS及统计数据处理》选修课的教学设计研究

结合医学统计学教学的实际,主要介绍了选修课程《SPSS及统计数据处理》在课堂教学中如何进行教学目标、教学内容、教学策略和教学效果评价的设计,并对教学实施情况进行了总结和思考。     

Association between the MnSOD Ala-9Val polymorphism and development of schizophrenia and abnormal involuntary movements in the Xhosa population

Reactive oxygen species (ROS)-mediated damage has been hypothesized to play a role in the development and poor outcome of schizophrenia, as well as the development of neuroleptic-induced abnormal involuntary movements. Recently, the functional polymorphism (Ala-9Val) in the manganese superoxide dismutase (MnSOD) gene (part of the antioxidant defense mechanism) was found to be associated with schizophrenia in a Turkish population. This study was aimed at replicating this finding in a Xhosa population. In addition, the role of Ala-9Val in abnormal involuntary movement and tardive dyskinesia development in the Xhosa population was also investigated. The schizophrenic patient group (n=286) and a healthy control group (n=243) were genotyped for the Ala-9Val polymorphism using heteroduplex-single stranded conformational polymorphism (HEX-SSCP) analysis. No significant difference in genotype or allele frequency could be observed between the schizophrenia and control group (P=0.294 and P=0.528 respectively). In addition no association could be found between the polymorphism and symptom severity (SANS and SAPS). The Xhosa schizophrenia patient group with abnormal involuntary movements (n=54) and a subgroup with tardive dyskinesia (n=30) was found to significantly differ in Ala-9Val genotype frequency (P=0.008 and P=0.011 respectively) compared to the Xhosa schizophrenia patient group without abnormal involuntary movements (n=204). However, no significant difference was found for the allele frequencies (P=0.955 and P=0.161). Further, using ANCOVA no association was found between AIMS score and genotype in the group with abnormal involuntary movements (P=0.1234). However, in the patient group with tardive dyskinesia an association was observed between genotype and AIMS score (P=0.0365). These results do not support a major role of the MnSOD Ala-9Val polymorphism in the development of schizophrenia or symptom severity in the Xhosa population. Yet it seems to be involved in the development of abnormal involuntary movements and tardive dyskinesia and may even modulate the severity of tardive dyskinesia.