Genomic sequences comparison and differential expression of miiuy croaker MHC class I gene, after infection by Vibrio anguillarum

Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. MHC gene family contains two main subgroups of immunologically active molecules. In order to study the molecular function and genomic characteristic of class I gene in teleost, the full lengths of MHC class Iα cDNA and genomic sequence were cloned from miiuy croaker (Miichthys miiuy). Seven exons and six introns were identified in miiuy croaker class Iα gene. This genomic structural feature of miiuy croaker is similar to that present in some fishes such as Japanese flounder and Atlantic salmon, but different from that present in some other fishes such as half-smooth tongue sole and channel catfish. The deduced amino acid sequence of class Iα gene had 25.9-54.1% identity with those of mammal and teleost. Real-time quantitative RT-PCR demonstrated that the MHC class Iα gene was ubiquitously expressed in 10 normal tissues; expression levels of MHC Iα gene were found first upregulated and then downregulated throughout the pathogenic bacteria infection process in spleen and kidney.     

液质联用快速确定尼群地平原料药中的杂质

目的:利用液质联用技术对尼群地平原料药中的2个杂质进行在线的质谱分析。方法:采用C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水(70︰30),流速为1.0 mL.min-1;Agilent 6320质谱仪,离子源为ESI,质量分析器为离子阱,检测模式为正离子。结果:直接推断出了2个杂质可能的化学结构。结论:检测结果对于尼群地平原料药的杂质分析、质量控制和合成工艺的改进具有重要作用。     

硫普罗宁注射液其他杂质的结构分析

目的:确证硫普罗宁注射液中2个较大杂质的结构。方法:利用HPLC和LC-MS等方法确证结构。结果:确证两未知杂质结构为:2-巯基丙酸和N-(2-氯丙酰基)-甘氨酸。结论:2个杂质的结构确定,为硫普罗宁生产工艺的改进及选择合适的运输、储存条件提供了重要信息。     

消旋棉酚晶态结构的研究

目的:研究消旋棉酚晶形结构。方法:采用红外光谱(IR)、差示扫描量热法(DSC)、测定溶解度和粉末X射线衍射法(XRD)来研究消旋棉酚晶态结构。结果:消旋棉酚[(±)G]、左旋棉酚[(-)G]和右旋棉酚[(+)G]的红外谱图基本相同;DSC图谱及热特征数据不一致;在消旋棉酚的饱和溶液中加入光学活性棉酚,光学活性棉酚可以继续溶解,并且有旋光产生;(±)G与(-)G及(+)G的XRD图完全不同。结论:消旋棉酚晶体属于外消旋化合物。     

液相色谱-质谱联用技术鉴定奥美沙坦酯有关物质

目的:采用液相色谱-质谱联用技术对奥美沙坦酯中有关物质进行结构鉴定。方法:采用LiChrospher C8(250 mm×4.6 mm,5μm)色谱柱,以甲醇-醋酸铵缓冲液为流动相梯度洗脱,对奥美沙坦酯有关物质进行分离;采用LC-MS/MS电喷雾正离子化测定各有关物质的相对分子质量和二级质谱,并进行结构解析。结果:检测到奥美沙坦酯粗品中11个有关物质,并推测出它们的化学结构均为奥美沙坦母核结构未发生变化的不同取代咪唑和四氮唑联苯衍生物。结论:色谱-质谱联用技术能够有效地用于药物中有关物质的鉴定,奥美沙坦酯有关物质研究可为其质量控制和工艺优化提供参考依据。     

Confirmatory factor analysis of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R)

The main objective of this study was to evaluate the factor structure of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R; Cooper, 1994) in a sample of 584 university students. A secondary goal was to investigate the relationships between motives and measures of alcohol use and drinking problems. The DMQ-R assesses four motive dimensions: social, enhancement, coping, and conformity. Confirmatory factor analysis supported a revised four-factor model identical to the original, with the exception of one item that did not load on its intended factor. Relative to coping and conformity motives, enhancement and social motives were strongly related to both alcohol use and drinking problems. Overall results indicate that the factor structure of the Brazilian DMQ-R parallels that observed in North America and Europe using the original English language DMQ-R, despite a distinct pattern of relationships with alcohol use and drinking problems.     

LC-MS/MS法鉴定注射用多西他赛中的有关物质

采用LC-MS/MS法对注射用多西他赛中的有关物质进行鉴定。采用乙酸乙酯提取注射剂,以去除制剂中影响LC-MS/MS离子化效果的磺丁基-β-环糊精等辅料,然后对提取物中的有关物质进行LC-MS/MS鉴定。采用LC-ESI-MS/MS测定各有关物质的一级和二级质谱图,并对准分子离子的各个产物离子进行归属,对各杂质可能的结构进行推测。在所建立的条件下,多西他赛及其有关物质分离良好,共鉴定了注射用多西他赛中的9个有关物质结构,其中4个有关物质为首次在注射用多西他赛中发现。本文所建立的LC-MS/MS法可以有效地分离分析多西他赛及其有关物质,为其制剂的质量控制和工艺优化提供参考。     

基于B/S架构的远程网络考试系统设计

为了合理利用场地,提高考试的效率和质量,为考生提供一个公平公正的考试环境,利用网络和数据库技术,设计了基于B/S架构的远程网络考试系统,可满足稳定性及可靠性等方面的需求,是现代教育技术的一个具体实现,具有重要的现实意义.     

医疗信息成熟度评价模型的构建

网络时代的发展促进了全球信息化,医院的信息化建设己成必然趋势,医疗信息己成为医院应对竞争的重要武器。为更好地解决医院信息化建设时的困难,加速我国信息化建设的发展,从成熟度的量化概念出发,提出医疗信息成熟度这一概念,并借鉴SW-CMM模型的分析方法,对其进行等级测评,构建评价模型。     

Molecular dynamics in drug design: new generations of compstatin analogs

We report the computational and rational design of new generations of potential peptide-based inhibitors of the complement protein C3 from the compstatin family. The binding efficacy of the peptides is tested by extensive molecular dynamics-based structural and physicochemical analysis, using 32 atomic detail trajectories in explicit water for 22 peptides bound to human, rat or mouse target protein C3, with a total of 257 ns. The criteria for the new design are: (i) optimization for C3 affinity and for the balance between hydrophobicity and polarity to improve solubility compared to known compstatin analogs; and (ii) development of dual specificity, human-rat/mouse C3 inhibitors, which could be used in animal disease models. Three of the new analogs are analyzed in more detail as they possess strong and novel binding characteristics and are promising candidates for further optimization. This work paves the way for the development of an improved therapeutic for age-related macular degeneration, and other complement system-mediated diseases, compared to known compstatin variants.