上颌窦提升术中单独应用Bio-oss骨胶原的临床评价

目的:评价骨胶原在上颌窦底植骨提升中的应用.方法:采用Bio-oss骨胶原对4例患者共6侧上颌窦行植骨提升术中,同期行牙种植术.结果:术后6个月X线检查发现,移植材料和移植物周围正常骨组织界线变模糊.所植入12 枚种植体无出现松动脱落.种植体均和形成的骨组织形成良好的骨组织,种植体周围未见明显骨吸收阴影,成功率100%.结论:Bio-oss骨胶原在上颌窦底提升术中单独作为植骨材料是可行的.     

胶原基纳米骨修复兔下颌骨缺损的实验研究

目的 研究胶原基纳米骨(nHAC)修复下颌骨缺损的效果,以期为临床提供一种理想的骨替代材料。方法 在32只成年兔双侧下颌骨形成0.8 mm×0.6mm全层骨膜骨质缺损,每一缺损作为一个实验单位。术后4、5、6、8、10、11、12周取材,行大体标本、X线片、组织学和扫描电镜观察。结果 nHAC与CPC相比同期成骨量大。8周时nHAC成骨情况尚不如Bio-Oss。而12周时已接近Bio-Oss成骨水平。空白缺损则未能修复。结论 nHAC生物相容性好,效果较好于CPC,远期与Bio-Oss相似,是一种较为理想的骨替代材料。     

新型nHA/PLGA纳米复合微球对大鼠颅骨缺损的修复作用

目的 探讨新型纳米羟基磷灰石（nHA）/聚丙交酯-乙交酯（PLGA）纳米复合微球对大鼠颅骨缺损的修复作用,为该材料的临床应用提供依据。     

不同的拔牙创处理技术在拔牙后牙槽突骨量保存中的作用

目的：通过动物实验探讨不同的拔牙创处理方法在预防拔牙后牙槽骨吸收中的作用。方法：6条杂种犬分别拔除两侧下颌第一第二双尖牙,测量缺牙处牙槽骨的高度和宽度。随机在一拔牙窝中置入Bi o-Oss骨粉,另一拔牙窝血凝块形成自然愈合。两侧创口分别覆盖胶原膜和颊侧牙龈翻瓣后拉拢缝合。三个月后取材测量缺牙区牙槽骨的高度和宽度。结果：置骨粉组无论覆盖胶原膜还是翻瓣后牙龈拉拢缝合的牙槽骨高度和宽度的丧失均比自然愈合组小。而覆盖胶原膜组和牙龈拉拢缝合组相比骨量变化没有统计学差异。结论：置入Bi o-Oss骨粉可以有效预防拔牙后牙槽骨的吸收,拔牙创口的不同处理方法对牙槽骨吸收无明显影响。     

Histological comparison of healing following tooth extraction with ridge preservation using enamel matrix derivatives versus Bio-Oss Collagen: a pilot study

The goal of the present clinical study was to evaluate new bone formation in human extraction sockets augmented with enamel matrix derivatives (EMD) and Bio-Oss Collagen. Patients with symmetrical single-rooted teeth in the bilateral quadrants of the upper jaw condemned for extraction participated in this study. Following extraction, the sockets (20 sockets) were randomly augmented using either EMD or Bio-Oss Collagen. After 3 months of healing, bone biopsies were obtained and prepared for histological analyses. Dental implants were then placed. Implant stability quotient (ISQ) readings were obtained for each implant at the time of surgery and at 1 and 3 months postoperatively. The mean new bone formation was 34.57 ± 25.67% in the EMD sites and 28.80 ± 16.14% in the Bio-Oss Collagen sites. There was no significant difference between the groups. The ISQ values were significantly higher for the implants placed in the EMD sites at the first and third months, but no significant differences were observed in the ISQ values for the implants placed in the Bio-Oss Collagen sites. The augmentation of the extraction sockets with EMD or Bio-Oss Collagen leads to similar behaviour in bone regeneration.     

Bio-Oss联合Bio-Gide治疗牙周炎垂直型骨吸收的临床疗效观察

目的评价异种骨移植物Bio-Oss联合Bio-Gide治疗牙周骨下袋的临床疗效。方法选择28例全身健康的慢性牙周炎患者,经基础治疗6周以上,牙周袋深度超过5mm的垂直型骨吸收患牙40牙位,随机平分为2组。引导组织再生GTR组20个牙位,在翻瓣术同时植入Bio-Oss和Bio-Gide。对照组20个牙位仅行翻瓣术;术后3、6、12个月回访,检查探诊出血指数、菌斑指数、牙周探诊深度、临床附着丧失、牙龈退缩和牙松动度,及X线检查比较2组骨缺损修复情况。结果 GTR组术后3、6、12个月牙周探诊深度、临床附着丧失、探诊出血指数均比术前及对照组明显改善（P〈0.01）;术后6、12个月牙松动度比术前及对照组明显减轻;术后3、6、12月菌斑指数比术前轻度降低（P〈0.05）。对照组术后3、6、12个月牙周探诊深度和探诊出血指数较术前减少,临床附着丧失和菌斑指数较术前轻度降低,牙松动度和龈退缩与术前差异无显著性。结论 Bio-Oss联合Bio-Gide能明显减轻牙周袋深度和减少牙周附着丧失,可促进牙周形成新附着和新骨。     

微动力系统及人工骨粉移植在下颌阻生智齿拔除术中的临床应用

目的：研究人工骨粉移植对下颌智齿拔除后第二磨牙远中牙槽骨缺损的修复及降低牙敏感发生的应用。方法：对73例存在近中牙槽间隔缺损的下颌近中低位阻生智齿采用微动力系统进行微创拔除术,对拔牙后的拔牙窝随机分为二组,植骨组38颗患牙采用微创拔牙后即刻植入Bio-OsSC01legen骨胶原质并缝合,对照组35颗患牙采用微创拔牙后常规复位缝合,对比两组的术中并发症、术后1周愈合情况、术后1、3个月第二磨牙远中牙槽嵴高度、下颌第二磨牙牙本质敏感发生率。结果：术中、术后1周两组无明显差异。术后1、3个月下颌第二磨牙牙本质敏感发生率在植骨组比对照组低,在统计学上有显著性差别（P〈0．05）;在植骨组不良反应明显比对照组少（P〈0．05）。应用微动力系统拔除下颌阻生智齿术中术后严重并发症发生率较低,对硬组织的损伤较小。结论：人工骨粉移植与人体相容性好,且可以有效降低下颌阻生智齿术后邻牙松动、牙本质敏感的发生。     

A prospective study on the effectiveness of newly developed autogenous tooth bone graft material for sinus bone graft procedure

PURPOSE

The purpose of this prospective study was to evaluate the effectiveness of newly developed autogenous tooth bone graft material (AutoBT)application for sinus bone graft procedure.

MATERIALS AND METHODS

The patients with less than 5.0 mm of residual bone height in maxillary posterior area were enrolled. For the sinus bone graft procedure, Bio-Oss was grafted in control group and AutoBT powder was grafted in experimental group. Clinical and radiographic examination were done for the comparison of grafted materials in sinus cavity between groups. At 4 months after sinus bone graft procedure, biopsy specimens were analyzed by microcomputed tomography and histomorphometric examination for the evaluation of healing state of bone graft site.

RESULTS

In CT evaluation, there was no difference in bone density, bone height and sinus membrane thickness between groups. In microCT analysis, there was no difference in total bone volume, new bone volume, bone mineral density of new bone between groups. There was significant difference trabecular thickness (0.07 µm in Bio-Oss group Vs. 0.08 µm in AutoBT group) (P=.006). In histomorphometric analysis, there was no difference in new bone formation, residual graft material, bone marrow space between groups. There was significant difference osteoid thickness (8.35 µm in Bio-Oss group Vs. 13.12 µm in AutoBT group) (P=.025).

CONCLUSION

AutoBT could be considered a viable alternative to the autogenous bone or other bone graft materials in sinus bone graft procedure.     

Bio-Oss骨复合富血小板纤维蛋白修复兔牙周骨缺损

目的探讨组织工程骨Bio-Oss骨复合富血小板纤维蛋白（PRF）修复牙周骨缺损中骨形成蛋白2（BMP-2）、骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）的表达及意义。 方法3月龄雄性新西兰大白兔36只,采用随机数表法分为4组,每组9只,制备单侧牙周骨缺损模型,于骨缺损处分别植入Bio-Oss骨（Bio-Oss组）、PRF（PRF组）和Bio-Oss/PRF复合物（Bio-Oss/PRF组）,以未植入任何材料者作为空白对照。术后4、8和12周处死动物,行大体观察、Masson染色及BMP-2、OPG、RANKL免疫组化观察,并对其表达进行析因设计的方差分析。 结果Masson染色结果显示,Bio-Oss/PRF组术后8周时见部分成熟骨,12周见骨板形成,骨成熟度较高。析因分析显示Bio-Oss组在4、8和12周时BMP-2表达量逐渐增高,差异有统计学意义（F = 51.30,P<0.001）;OPG表达量随时间延长先升高后减低,差异有统计学意义（F = 167.03,P<0.001）;RANKL表达量随时间延长逐渐减低,差异有统计学意义（F = 5.39,P = 0.046）。PRF组在4、8和12周时BMP-2表达量无统计学意义（F = 0.68,P = 0.544）;OPG和RANKL表达量随时间延长逐渐减低,差异有统计学意义（F_OPG = 1070.93,P_OPG<0.001;F_RANKL = 2306.15,P_RANKL<0.001）。Bio-Oss/PRF组在4、8和12周时BMP-2、OPG和RANKL表达量逐渐降低,差异有统计学意义（F_BMP-2 = 13.29,P_BMP-2<0.001;F_OPG = 237.91,P_OPG<0.001;F_RANKL = 132.48,P_RANKL<0.001）。空白对照组在4、8和12周时BMP-2和OPG表达量先升高后减低,差异有统计学意义（F_BMP-2 = 88.33,P_BMP-2<0.001;F_OPG = 30.06,P_OPG<0.001）,RANKL表达量随时间推移逐渐减低,差异有统计学意义（F = 56.52,P<0.001）。 结论Bio-Oss骨复合PRF应用可促进成骨以修复牙周骨缺损。     

Bio-Oss混合自体骨移植联合膜引导骨再生术在种植中的临床应用

目的：观察Bio-Oss混合自体骨联合膜引导骨再生术应用于牙种植的临床疗效。方法：对临床21例骨量不足的患者Ⅰ期植入种植体，同时植入Bio-Oss与自体骨的混合物，表面覆盖Bio-Gide胶原膜。在术后十天，一月，三月，六月通过临床检查及X线检查评价临床疗效。结果：21例患者植骨区新骨形成良好，牙槽嵴的外形丰满，种植体无动度，均完成了上部修复。结论：Bio-Oss混合自体骨联合膜引导骨再生术应用于种植区骨量不足的患者可取得较好的疗效。