Lipid Peroxidation and Vitamin E in Red Blood Cells and Plasma in Hemodialysis Patients Under rhEPO Treatment

Abstract: Lipid peroxidation, measured by malonyldial-dehyde (MDA) and vitamin E in red blood cells (RBC) and plasma, was investigated in 25 hemodialysis (HD) patients before and after 6 months rhEPO therapy. RBC-MDA was significantly elevated, but plasma MDA was in the reference range. After recombinant human erythro-poietin (rhEPO) treatment, the MDA level was significantly decreased in both compartments. Marked vitamin E deficiency was established in RBC as well as in plasma. rhEPO therapy restored vitamin E levels in both compartments. Our data suggest a possible positive rhEPO-antioxidant effect in HD patients.     

比较异丙酚和依托咪酯对心脏瓣膜置换术患者过氧化脂质和超氧化物歧化酶的影响

目的:观察异丙酚对心脏瓣膜置换术患者过氧化脂质和超氧化物歧化酶的影响。方法:20例瓣膜置换术患者分为异丙酚组和依托咪酯组。分别在心肌缺血再灌注前后取血做过氧化脂质和超氧化物歧化酶测定,同时记录心电图和平均动脉压。结果:过氧化脂质在异丙酚组主动脉插管时下降,主动脉开放后升高,均有显著差异(P<0.05,P<0.01)。依托咪酯组主动脉开放后明显升高,亦高于异丙酚组同时值,差异均显著(P<0.01)。超氧化物歧化酶主动脉开放后两组均下降(P<0.05,P<0.01),两组间无差异。主动脉开放后心律失常、心肌缺血、电击复跳例数异丙酚组少于依托咪酯组。结论:异丙酚可明显降低心肌缺血再灌注后过氧化脂质含量,减少心律失常和心肌缺血现象。     

煤石英对大鼠肺泡巨噬细胞氧化系统的影响

利用染煤石英混合粉尘大鼠作为实验模型，研究在实验性大鼠煤矽肺形成过程中肺泡巨噬细胞氧化和抗氧化系统的改变。结果表明在染煤石英粉尘７天，脂质过氧化产物有轻度升高，至３０天时己恢复正常，超氧化物歧化酶在染尘后７天降低，以后恢复至正常，而还原型谷胱甘肽和谷胱甘肽过氧化物酶升高明显，这与石英所致肺泡巨噬细胞的变化明显不同，说明脂质过氧化反应在煤矽肺形成过程中作用不明显。     

肉苁蓉多糖对衰老小鼠脂质过氧化的影响

目的:研究肉苁蓉多糖(polysacchridesofcistanchedeserticolaY.C.Ma,PCD)对亚急性衰老小鼠的抗 脂质过氧化作用。方法:使用D 半乳糖造成小鼠亚急性衰老模型,观察PCD对亚急性衰老小鼠血液和肝脏组 织中超氧化物岐酶(SOD)、脂质过氧化物(LPO)的含量的影响。结果:灌服肉苁蓉多糖衰老小鼠的血液和肝脏 组织中SOD明显高于模型组(P<0.05);LPO的含量明显低于模型组(P<0.05)。结论:肉苁蓉多糖具有明显 的抗脂质过氧化功能,防止亚急性衰老小鼠的组织脂质过氧化损伤。     

表没食子儿茶素没食子酸酯对缺血再灌注损伤大鼠心肌脂质过氧化及心肌酶活性的影响

目的：观察表没食子儿茶表没食子酸酯（EGCG）对缺血再灌注损伤大鼠心肌脂质过氧化及心肌酶活性的影响。方法：雄性Wistar大鼠50只分为5组，假手术组、缺血再灌注组（IR），丹参（SM，100mg/kg）组，EGCG1（10mg/kg)组和EGCG2（20mg/kg）组，每组10只动物，采用结扎大鼠左冠状动脉前降支30min后再灌注60min的方法建立在体缺血再灌注损伤模型，分别测定心肌丙二醛（MDA）含量，超氧化物歧化酶（SOD）及ATP酶活性和血浆乳酸脱氢酶（LDH）水平。结果：与假手术组比，IR组大鼠心肌MDA及血浆LDH明显升高，SOD及ATP酶活性则显著降低（P＜0．01）；EGCG和SM均能显著，降低MDA与LDH，明显增加SOD与ATP酶活性（与IR组比，P＜0．01），并且EGCG（20mg/kg)，降低MDA与LDH的作用较SM（100mg/kg)更明显（P＜0．05）。结论：EGCG通过抑制脂过氧化反应和提高心肌SOD与ATP酶活性，对大鼠缺血再灌注损伤心肌有显著的保护作用。     

蜂花粉醇提物对小鼠体外生成过氧化脂质的影响

组织器官的衰老与过氧化脂质的生成密切相关，本实验证明蜂花粉醇提物有明显的对抗小鼠肝、肾、脑组织在体外生成过氧化脂质的作用，证明蜂花粉具有延缓衰老的作用。     

Effect of pentoxifylline on veno-occlusive priapism-induced corporeal tissule lipid peroxidation in a rat model

Objective: To investigate whether pentoxifylline could play a role in attenuation of the hazardous effects of ischemia/reperfusion on corporeal tissue in a rat model of veno-occlusive priapism (VOP). Materials and methods: Placebo and pentoxifylline were given to eight groups of rats prior to priapism being induced by a vacuum constrictive device for durations of 6 and 12 h, respectively. Half of the groups of rats that underwent the same duration of priapism (ischemic) were subjected to 1 h of detumescence after band removal (reperfusion). One group underwent no manipulation and no drug administration and served as a baseline determination (control). Corporeal homogenates were examined for lipid peroxidation (LP) derived malondialdehyde (MDA) accumulation via thiobarbituric acid assay. Results: MDA concentration differed significantly between VOP rats and controls (P < 0.001) but did not differ significantly between ischemic-only groups and reperfused groups (P > 0.05). In the pentoxifyllinepretreated groups, although MDA accumulation tended to be slightly lower than in the placebo groups, the difference was not statistically significant (P > 0.05) either in the 6- or 12-h duration priapic groups. Conclusions: LP, an indicator of radical oxygen metabolite (ROM) induced injury, occurs in rat corporeal tissue during and after abolishment of VOP. Single-dose pentoxifylline pretreatment failed to exert a protective effect on corporeal tissue in a rat model of VOP in terms of attenuation of LP.     

硫酸锌对肠缺血—再灌注损伤的防治作用

目的：探讨硫酸锌对肠缺血－再灌注（ischemia-reperfusion,I/R)损伤的防治作用及其机制。（2）方法：复制家兔肠I／R损伤模型，观察硫酸锌对超氧化物歧化酶（SOD），黄嘌呤氧化酶（XO）及丙二醛（MDA）含量的影响，实验分为I／R损伤组，硫酸锌＋I／R组和假手术组（Sham)对照组，并进行比较。（3）结果：缺血前3组动物红细胞SOD、红细胞膜及血浆MDA，血浆XO值均无统计学差异，I／R组再灌注后2h与缺血前及Sham组相比，SOD活性显下降，XO活性及MDA含量明显增加（P＜0．01），此外，肠、肺、肝等组织MDA含量亦明显高于Sham组（P＜0．01），静脉给予硫酸锌，可使XO及MDA含量降低，且可防止SOD减少。结论：肠I／R过程中伴有多器官脂质过氧化损伤，硫酸锌通过抗脂质过氧化能减轻或在一定程度上避免再灌注损伤的进一步加剧。     

黄疸乳糜对血液细胞分析结果的影响

目的：探讨黄疸、乳糜对血液细胞分析结果的影响。方法：制备含一定浓度梯度胆红素、甘油三酯的全血标本，在血细胞分析仪上进行测定，同时对临床乳糜标本加以检测。结果：黄疸标本分析结果中血小板计数降低，与胆红素浓度呈负相关，相关系数为-0．9925；当胆红素≥547．2μmol／L时，血红蛋白测定受干扰。而乳糜标本的分析结果中，血红蛋白浓度假性增高，相关系数为0．9991；其他参数基本不受影响。结论：黄疸、乳糜均对血细胞分析的部分参数产生影响。