Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells

Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by culturedhuman arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected,and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electro-phoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultravioletspectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance     

突触小泡膜表面突起与微管联系的超微结构研究

本研究用透射电镜技术观察了大脑皮质（视区）和海马（CA1区和CA3区）的神经终末内的突触小泡和微管间的联系。结果揭示：在适当的超薄切片样品上，常显示出突触小泡膜表面上有短的突起，说明突触小泡是借其膜表面的短突与其它小泡和微管进行联系的。除小泡外，在微管的表面也见一些短突并与小泡发生接触。本文首次报道了用透射电镜方法观察脑内小泡-微管借表面短突连接的联系，提示突触小泡膜表面和微管表面的短突在轴浆运输机制中负有重要的作用。     

髌骨关节面的应用解剖学研究

本文利用150例髌骨(干燥髌骨100例,福尔马林固定膝关节内的髌骨50例)观测了髌骨关节面和关节软骨的形态及其与股骨内外侧髁关节面在屈膝0～135度范围的对应关系。结果发现中间纵嵴向后突起并由下向上逐渐变凸占100％;内侧纵嵴明显突起者占62％,此时其髌骨内侧部关节面与股骨关节面之间的间隙较大;在膝关节屈曲过程申两关节面接触的顺序为髌骨关节面自下而上、股骨内外侧关节面从前向后相对滑动,伸膝时则相反;髌骨关节面的表面积为1019±20(650～1540)mm~2。并讨论了髌骨关节面的解剖学特点及其临床意义。     

羊膜建库及其临床应用研究

目的 :制备与保存羊膜 (Amnioticmembrane,AM ) ,为临床移植治疗眼表泪液 (Ocularsur face&Teardisease ,OSTD)提供新鲜羊膜 (Freshamnioticmembrane ,FSAM )、冻干羊膜 (Frozenam nioticmembrane ,FZAM )并观察其眼表重建的特点与临床疗效。方法 :采用目前国际公认的羊膜制备与保存方法 ,制备FSAM、FZAM并进行保存研究 ,经组织染色和电子显微镜检查 ,观察保存羊膜的形态及其活性 ;并将FSAM、FZAM应用于 12例OSTD患者 ,行患眼FSAM或FZAM移植术。通过术后印迹细胞学追踪观察移植后AM上皮细胞存活与移行、替代时间 ,评估AM移植重建眼表的临床疗效。结果 :FSAM、FZAM在光镜和电镜下均与正常球结膜组织结构相近 ,主要结构为胶原纤维和网状纤维。 12例OSTD患者行羊膜移植术 (Amnioticmembranetransplantation ,AMT)后均未见明显急性排斥反应 ,术后 1～ 2周可见少量新生血管长入植片 ,AMT后印迹细胞学追踪检查 ,术后 3个月为阴性 ,4个月出现阳性反应。所有患者术后随访 5～ 18个月 ,平均 11个月 ,AM在术后 4～ 9个月逐渐溶解、消失 ,移植区眼表的色泽与结构基本恢复正常。结论 :FSAM、FZAM是目前理想的结膜替代材料并可有效地用于重建角、结膜表面 ,减轻炎症反应 ,减少新生血管的生成 ,抑制纤维组织增生 ,防止     

Distributive profiles of free and protein-incorporated tryptophan and valine in cerebral cortex slices from adult and 2-day-old rats

The distribution of the specific radioactivity and the incorporation into protein of [³H]-tryptophan and [³H]valine at varying layers from surface to centre were measured in incubated slices of cerebral cortices from infant and adult rats. Specific radioactivity in free amino acids was in both age groups highest in the intact surface layer. Incorporation of tryptophan into protein was even in slices from adult rats but much less than the average in the surface layers in slices from infant rats. Incorporation of valine exhibited similar heterogeneity in both age groups. The results suggest in brain slice preparations a zonal compartmentation of amino acid and protein metabolism which varies for different amino acids.     

表面改性在介入器械上的应用

论述赋予介入器械的材料表面润滑性、抗菌性、抗凝血性及抗组织增生等性能的各种表面改性技术的现状，讨论这些技术的一些应用的实例，提出需要进一步研究的问题。     

云纹技术在断裂分析中的应用

改进了传统的云纹数据处理方法,提出用曲面拟合方法处理云纹数据,提高了计算机处理云纹数据的效率与质量。又提出用云纹法测量J积分的技术,可望解决实际工程构件的J积分测量问题。     

Transmission electron microscopy of early microbial colonization of human enamel and root surfaces in vivo

Abstract – This study describes the early microbial colomization of teeth by the use of light-and transmission electron microscopy. Six dental students carried a total of 60 test pieces of unerupted enamel and root surface in intraoral acrylic appliances for 4, 8, 12, 24 and 48 h, during which periods oral hygiene was abandoned. Pronounced variations were recorded in structure and thickness of the pellicle across the individual surfaces of both dental tissues. Bacterial single-cell colonization increased the electron density of the adjoining pellicle. Micro-colonies of bacteria were observed in relation to enamel surface irregularities such as perikymata, while the distribution on root surfaces appeared incidental. Root surfaces were generally colonized by thicker deposits than homologous enamel surfaces although the structural composition of the microbiota was similar. Gram-positive bacteria with thick cell walls appeared in coccoid or rod-shaped configurations depending on the age of the bacterial deposit. These bacteria were further characterized by selective invasion between collagen fibers. After 48 h the complexity of the microbiota was increased by the establishment of new bacterial species in the superficial layer. It is concluded that the pattern and composition of the early microbiota on teeth is more complex and variable than hitherto assumed.     

乙型肝炎病毒PreS1抗原与HBV感染和复制的相关性研究

目的研究血清前S1抗原（PreS1）与乙型肝炎病毒（HBV）感染和复制的相关性。方法同时测定458例乙型肝炎患者和35例健康对照者血清HBV—DNA载量、PreS1和HBeAg。以HBV-DNA载量为标准，进行PreS1和HBeAg比较。结果HBV-DNA载量小于10^3拷贝／ml的223例中PreS1阳性102例（45．74％），HBeAg阳性16例（7．17％）；PreS1和HBeAg与HBV-DNA载量等级的线性相关系数分别为0．9329和0．9718；PreS1和HBeAg诊断乙型肝炎病毒感染的敏感性分别为72．77％、52．34％，特异性分别为54．26％、92．82％，阳性预测值分别为62．64％、88．49％，阴性预测值分别为65．41％、64．89％，准确率分别为63．76％、72．05％。结论随HBV—DNA载量的升高，HBeAg的检出率显著提高，PreS1的检出率次之；PreS1与HBV-DNA有一定相关性，但其相关程度不及HBeAg。