尖吻蝮蛇毒中一种新类凝血酶的分离纯化

用阴离子交换色谱和凝胶过滤色谱技术,从尖吻蝮蛇毒中纯化得到一个具有凝血活性的组分.经Superdex 75 HR10/30预装柱检测,纯度达99.91%.SDS-PAGE显示为单一条带,还原、非还原条件下分子量分别为59.25k、52.58k.该组分的凝血酶比活为41.5u/mg,精氨酸酯酶比活为14.29u/mg,但不激活血浆凝血因子ⅩⅢ.EDTA不能抑制其凝血活性,而苯甲磺酰氟则产生不可逆抑制作用.结果表明该酶是一种新尖吻蝮蛇毒类凝血酶.     

重组人干扰素α-2b两步柱色谱分离纯化工艺研究

通过改进柱色谱条件,两步柱色谱分离纯化重组人干扰素α-2b,简化了工艺,缩短了生产流程,纯化倍率达到5.6倍,纯度98%.蛋白比活性1.43×108IU/mg蛋白,活性回收率58%.     

那格列奈及其片剂中L-异构体的HPLC测定

建立了HPLC法测定那格列奈及其片剂中的杂质L-异构体的含量.采用以α-酸性糖蛋白键合硅胶为固定相的手性色谱柱,0.02mol/L磷酸钾缓冲液(pH 7.1)-乙腈(98:2)为流动相,检测波长210nm,L-异构体检测限为2ng,平均回收率为98.0%.     

中国地理资源期刊集群平台期刊特约专栏实施成效分析与启示

【目的】 依据中国地理资源期刊集群平台期刊特约专栏出版实践,分析实施成效,总结出版经验,为同行组织特约专栏提供借鉴。【方法】 采用中国知网的统计数据,分析中国地理资源期刊集群7种平台期刊在2016—2017年刊发的特约专栏的表现。【结果】 特约专栏整体上能够提高期刊影响力,推广学术传播,但不同类型的特约专栏的表现存在差别。【结论】 内容型专栏是提升期刊影响力的有效措施,双盲评审、相似主题的间隔时间及同一作者的出现频次是特约专栏成效的重要影响因素。特约专栏的组织需要与科技创新一致,落实“四个面向”的战略部署。     

Enrichment of Dystrophy-Associated Antigen from Erythrocyte Membranes of Mice with Muscular Dystrophy

Summary

In a previous report, it was shown that erythrocyte membranes from mice with muscular dystrophy of strain 129/ReJ-dy manifest a unique antigen. In the current study, this antigen was enriched from solubilized membranes by a two-step solid phase immunoadsorbent. The enriched fraction retained antigenicity as well as a reduced set of electrophoretic forms of protein over that seen in solubilized membranes. The enriched fraction contained no novel molecular weight species but several were noticeably enriched, particularly at 54,000 daltons.     

Acetabular fractures,anatomy and implications for treatment

Acetabular fractures are relatively uncommon and their definitive treatment tends to be focused on specialist major trauma centres. This can make both accurate diagnosis and management challenging, particularly in hospitals where they are rarely seen. Contemporary management of these injuries owes a lot to the work of Judet and Letournel undertaken in the 1970s. The key to understanding these injuries is to know the embryology and development of the pelvis and then be able to appreciate its three-dimensional structure from two-dimensional X-rays. This can then be overlaid with the Judet classification and the action of force vectors encountered in various mechanisms of injury. It is also important to realise the ageing demographics of this group of patients and the complexities this adds to classification and ultimately treatment. Finally, the presence of an acetabular fracture is often seen in association with a number of other injuries. It is imperative that these are appropriately and contemporaneously diagnosed so that a comprehensive management plan may be instituted to give the best outcomes. However, even with optimal management the prognosis is guarded with a majority of patients suffering some degree of functional loss and this must be made clear to the patient from the outset.     

3,5-O-二咖啡酰基奎宁酸的制备及其对HeLa细胞的抗增殖活性研究

目的 制备高纯度3，5-O-二咖啡酰基奎宁酸，并评价其对人宫颈癌HeLa细胞的抗增殖活性。方法 采用柱色谱提取法和中压液相色谱法从奇蒿花中分离、纯化得到高纯度的3，5-O-二咖啡酰基奎宁酸。采用MTT法评价该化合物对人宫颈癌HeLa细胞的体外抗增殖活性。结果 柱色谱提取的提取率和中压液相色谱法的回收率分别为99.0%，61.2%，总回收率为54.0%。随着3，5-O-二咖啡酰基奎宁酸浓度升高，HeLa细胞存活率下降，细胞形态损伤增加，受试药物的IC₅₀值为26.5µg·mL^-1。结论 本研究提供了一种简单、高效、节能的3，5-O-二咖啡酰基奎宁酸制备方法。3，5-O-二咖啡酰基奎宁酸对HeLa细胞具有一定的体外抗增殖活性。     

MR imaging in nelarabine-induced myelopathy

Nelarabine is one of the newer and novel drugs approved by the USA Food and Drug Administration for treatment of relapsed and resistant acute lymphoblastic leukemia. Although there are a few accounts of the neurologic toxicity of nelarabine in the oncological literature, it has never been discussed from a radiologic stand point to our knowledge. We describe a case of nelarabine-induced myelopathy and review the existing literature in an attempt to characterize the MRI features helpful in making an early diagnosis of this elusive entity.     

庆大霉素C组分和有关物质测定的HPLC-柱后衍生化法与脉冲安培电化学法的比较研究

摘 要 目的： 参考《欧洲药典》的HPLC 脉冲安培电化学法建立高效液相色谱 柱后衍生化法测定庆大霉素C组分含量及其有关物质, 同时与电化学法进行比较研究。方法: 采用亲水性HydrosphereC₁₈（250 mm×4.6 mm,5 μm）色谱柱,以含0.7%三氟乙酸及0.025%五氟丙酸的50 mmol·L^-1氢氧化钠溶液（pH2.6）-乙腈（98.5∶1.5）为流动相。衍生化法反应温度30℃,荧光激发波长340 nm,发射波长430 nm。电化学法采用脉冲安培检测器,金电极为工作电极,四电位波形工作模式。结果:两种方法测定的庆大霉素C组分（C_1a, C₂, C_2a和C₁）分别在5.82～233.00,6.92～277.00,4.00～160.00和6.23～249.00 μg·mL^-1的浓度范围内线性关系良好（r均≥0.999 3）,检测限和定量限范围分别为0.92～3.28 ng和1.37～5.19 ng。结论: 两种方法的检测结果无明显差异。