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31.
糖尿病肾脏胶原非酶糖化与氨基胍阿斯匹林的治疗作用 总被引:1,自引:0,他引:1
本实验系统观察了STZ所致糖尿病鼠在1、3、6个月后肾脏组织胶原发生非酶糖化的情况,及氨基胍、阿斯匹林对肾脏胶原非酶糖化的阻断作用。结果表明:各病程糖尿病动物肾组织的胶原含量和非酶糖化早期产物(5-HMF)及胶原相联荧光值均明显高于正常对照。氨基胍能够减弱糖尿病动物肾组织胶原含量的增加和荧光产物的生成,但对5-HMF含量无影响。阿斯匹林未显示出对胶原非酶糖化的影响。 相似文献
32.
Amir Tejani Ping Leung Ho Lea Emmett Donald M Stablein 《American journal of transplantation》2002,2(2):142-147
Chronic rejection accounted for 32% of all graft losses in 7123 pediatric transplants. In a previous study acute, multiple acute and late acute rejections were risk factors for the development of chronic rejection. We postulated that the recent decrease in acute rejections would translate into a lower risk for chronic rejection among patients with recent transplants. We reviewed our data on patients transplanted from 1995 to 2000, and using multivariate analysis and a proportional hazards model developed risk factors for patients whose grafts had failed due to chronic rejection. A late initial rejection increased the risk of chronic rejection graft failure 3.6-fold (p < 0.001), while a second rejection resulted in further increase of 4.2-fold (p < 0.001). Recipients who received less than 5 mg/kg of cyclosporine at 30 days post-transplant had a relative risk (RR) of 1.9 (p = 0.02). Patients transplanted from 1995 to 2000 had a significantly lower risk (RR = 0.54, p < 0.001) of graft failure from chronic rejection than those who received their transplants earlier (1987-94). Since we were able to demonstrate that there is a decreased risk of chronic rejection graft failure in our study cohort, we would conclude that the goal of future transplants should be to minimize acute rejections. 相似文献
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34.
探讨荧光原位杂交法(FISH)对母血中胎儿有核红细胞(NRBC)进行无创性产前诊断的可行性。20例孕龄15-20周的孕妇外周血经不连续密度梯度离心、制片、显微镜下识别并共计数NRBC及定位,然后行Y染色体的FISH检测。结果发现10例孕男性胎儿的孕妇外周血细胞涂片中每例均有阳性杂交信号出现;阳性率为60%(24/40)。10例孕女性胎儿的孕妇外周血细胞涂片中1例出现阳性杂交信号;阴性率为95%(38/40),假阳性率仅为5%(2/40)。结果提示FISH法对于用母血中分离到的胎儿细胞进行染色体异常的无创性产前遗传学检查具有重要意义。 相似文献
35.
用荧光原位杂交技术快速诊断孕早期胚胎染色体数目异常 总被引:4,自引:1,他引:3
目的探讨荧光原位杂交(FISH)技术在快速产前诊断孕早期胚胎染色体数目异常中的价值.方法采用13,18,21,X和Y染色体特异性DNA探针,对46例孕47~65 d高危孕妇的绒毛间期细胞进行FISH检测,同时行常规染色体核型分析平行诊断.结果与染色体核型分析结果一致的染色体数目正常43例,异常3例(47,XY 21;47,XY 18和45,X).3例异常核型胚胎经治疗性流产后再分别对其绒毛行常规染色体核型分析,结果与产前诊断相符.结论FISH技术用于产前诊断孕早期胚胎染色体数目异常具有快速、准确等优点. 相似文献
36.
目的应用间期荧光原位杂交技术(I-FISH)检测初诊急性白血病患者中核心结合因子(CBF)受累的染色体异常情况并对治疗后患者的微小残留病(MRD)进行监测,同时对I-FISH与常规染色体G显带的灵敏度进行比较。方法在骨髓形态学初筛的基础上,应用常规染色体G显带技术对15例急性髓系白血病(AML)进行核型分析,应用I-FISH对患者可能受累的CBF相关靶基因进行检测,其中7例患者治疗后进行了MRD监测。结果(1)正常对照组中,CYTOCELL公司提供的3种探针(AML1/ETO易位探针、MYH11基因断裂点双色探针和ETV6/AML1易位探针)的正常分界值分别为4.13%、1.95%和2.12%。(2)常规染色体G显带分析,15例患者中有6例伴有潜在累及CBF基因的染色体异常,其中8例AML-M2中5例伴t(8;21),2例AML-M4EO中1例伴inv(16),5例儿童B系急性淋巴细胞白血病(B-ALL)未检出相应的异常。I-FISH检测15例患者发现有12例累及CBF基因,其中8例AML-M2患者均为AML1/ETO融合基因(+),2例AML-M4EO病人CBFβ/MYH11融合基因均为(+),5例儿童B-ALL中2例ETV6/AML融合基因(+)。(3)3例AML-M2患者中2例MRD阳性;2例M4E0患者治疗后MRD监测结果均阴性;2例B-ALL患者1例阴性,1例阳性。结论I-FISH较常规染色体G显带技术能够更灵敏地检测出累及CBF的急性白血病,在初诊时联合应用这两种 相似文献
37.
Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane
fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity
induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression
of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that
the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs
against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However,
the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated
that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding
isotypic and idiotypic determinants of IgM on the cell membrane. 相似文献
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Using NADH fluorometry to monitor myocardial metabolism, the mechanism of reperfusion injury was investigated after the delivery of an experimental reperfusate. Using an isolated working heart preparation, rat hearts underwent 15 min of global ischemia at 37 degrees C. Following the ischemic insult, an oxygenated enriched reperfusion solution was given for 5 min. The hearts were then returned to a working state and aortic flow recorded to evaluate recovery. NADH levels were monitored throughout the experiment with a fluorometer and glycogen, AMP, ADP, and ATP were measured biochemically pre- and postischemia, after reperfusion and after recovery. In this study, reperfusion injury was best abated by an enriched reperfusate. Our results indicate the mechanism for this amelioration is not high-energy phosphate replenishment. Rather, as indicated by NADH fluorescence, the hearts attain an intermediate level of metabolism that permits glycogen to be restored and functional recovery to be improved. 相似文献