突变型HBV前C-C基因疫苗的构建及真核细胞表达

目的采用点突变技术对HBV前C—C基因中的蛋白酶切位点进行4处点突变,构建突变型HBV前C—C／ENHⅡ重组基因疫苗,转染HepG2细胞,研究其存真核细胞内的表达情况。方法采用单引物二次PCR法对质粒VEC依次进行基因点突变,得到151＋154（VE2）、151＋154＋164＋167点突变（VE4）2种突变型疫苗,转染HepG2细胞,通过细胞免疫化学、酶联免疫分析、免疫印迹等方法检测其在HepG2细胞内的表达情况及表达产物的分子量。结果VEC、VE2、VE4转染HepG2细胞均胞浆表达目的蛋白,产物分子量分别为18、20、22kD,细胞裂解液的HBeAg含量VE4、VE2高于VEC,上清中则反之。结诊安蛮刭HRV前C—C基因疫苗VE2、VE4构建成功并表达预期病毒前C蛋白前体。     

Comparison of aqueous solubility estimation from AQUAFAC and the GSE

The GSE (General Solubility Equation) and AQUAFAC (Aqueous Functional Group Activity Coefficients) are two empirical models for aqueous solubility prediction. This study compares the aqueous solubility estimation of a set of 1642 pharmaceutically and environmentally related compounds, using the two methods. The average absolute errors in the solubility prediction are 0.543 log units for AQUAFAC and 0.576 log units for the GSE. About 88.0% of the AQUAFAC solubilities and 83.0% of the GSE molar aqueous solubilities are predicted within one log unit of the observed values. The marginally greater accuracy of AQUAFAC is due to the fact that it utilizes fitted-parameters for many structural fragments and is based on experimental solubility data. The GSE on the other hand is a simpler, non-regression based equation which uses two parameters for solubility prediction.     

隐蔽区胃癌的影像检查与诊断

目的:探讨隐蔽区胃癌的影像检查技巧与诊断要点。方法:20例隐蔽区胃癌均行钡剂造影检查,发生于胃底后壁7例,胃底后壁小弯侧13例,其中贲门管受累9例。20例中16例术前行胃镜检查,全部病例均经胃肠钡餐造影检查。结果:20例胃底后壁癌19例手术切除,其中2例病变与脾脏粘连将脾脏一并切除。1例年龄大(74岁)心功能不全而保守治疗,随访2a肿瘤由隆起性病变形成巨大溃疡。20例病理报告均为腺癌。结论:隐蔽区胃癌因其发病位置相对特殊,前后左右相互重迭或病变位于贲门后上方,不论钡剂造影及胃镜检查均易漏诊或病变范围不易确定,因此,如何掌握本病的检查技巧是提高本病检出率的重要环节。     

Efficient oxidation of promutagenic hydroxymethylpyrenes by cDNA-expressed human alcohol dehydrogenase ADH2 and its inhibition by various agents

Alkylated polycyclic aromatic hydrocarbons can be metabolically activated via benzylic hydroxylation and sulphation to electrophilically reactive esters. However, we previously found that the predominant biotransformation route for the hepatocarcinogen 1-hydroxymethylpyrene (1-HMP) in the rat in vivo is the oxidation of the side chain by alcohol dehydrogenases (ADHs) and aldehyde dehydrogenases to the carboxylic acid. Inhibition of this pathway by ethanol (competing ADH substrate) or 4-methylpyrazole (ADH inhibitor) led to a dramatic increase in the 1-HMP-induced DNA adduct formation in rat tissues in the preceding study. In order to elucidate the role of individual ADHs in the metabolism of alkylated polycyclic aromatic hydrocarbons, we expressed the various members of the human ADH family in bacteria. Cytosolic preparations from bacteria expressing ADH2 clearly oxidized hydroxymethylpyrene isomers (1-, 2- and 4-HMP) with the highest rate. This form was purified to near homogeneity to perform detailed kinetic analyses. High catalytic efficiencies (V(max)/K(m)) were observed with HMPs. Thus, this value was 10,000-fold higher for 2-HMP than for the reference substrate, ethanol. The corresponding aldehydes were also efficiently reduced by ADH2. 4-Methylpyrazole inhibited the oxidation of the HMP isomers as well as the reverse reaction. Daidzein, cimetidine and the competing substrate ethanol were further compounds that inhibited the ADH2-mediated oxidative detoxification of 1-HMP.     

不同途径注射鼠神经生长因子对腓总神经损伤康复的疗效观察

目的 探讨腓总神经损伤患者营养神经药物最佳的给药途径.方法 40例腓总神经损伤患者随机分为2组,穴位注射组(20例)将鼠神经生长因子注射液以足三里、阳陵泉等穴位为进针点,通过穴位注射的方式给药,肌肉注射组(20例)将相同药物以臀大肌肌肉注射的方法给药,治疗15d、1个月后分别比较2组患者患侧肢体感觉功能、运动功能和腓总神经传导速度的差异.结果 穴位注射组对感觉障碍恢复、提高肌力、改善腓总神经传导速度的治疗效果明显高于臀大肌肌肉注射组,差异具有统计学意义(P＜0.05).结论 鼠神经生长因子穴位注射治疗效果优于常规肌肉注射,具有良好的推广应用价值,临床治疗中注意规范操作可取得更好的疗效.     

俞募配穴温针灸法联合补中益气汤治疗老年性慢性腹泻的临床观察

目的:探讨俞募配穴温针灸法联合补中益气汤治疗老年性慢性腹泻的临床疗效。方法:选取2017年8月至2018年9月西宁市妇幼保健计划生育服务中心收治的老年性慢性腹泻患者88例作为研究对象,将所有入组患者随机分为观察组与对照组,每组44例,对照组给予双歧杆菌四联活菌片口服治疗,观察组在对照组治疗的基础上联合补中益气汤和俞募配穴温针灸治疗2组患者以2周为1个疗程,连续进行2个疗程后进行疗效观察。观察治疗后2组临床症状改善情况及疗效,检测并比较治疗前后2组血清胃肠激素及脑肠肽水平。结果:与治疗前比较治疗后2组晨起泄泻、腹部冷痛、腰膝酸软、食欲不振和形寒肢冷评分均显著下降(P0.01)且观察组显著低于对照组(P0.01)。治疗后4周观察组总有效率为93.18%,对照组为77.27%,观察组总有效率高于对照组(P0.05)。治疗后2周、4周、3个月观察组气虚积分均显著低于对照组(P 0.05或P 0.01)。与治疗前比较,治疗后2组血清MOT、GAS、5-HT、SP水平均显著下降,血清VIP、CCGRP水平均显著升高(P 0.05或P 0.01),且组间差异有统计学意义(P 0.05或P 0.01)。结论:俞募配穴温针灸法联合补中益气汤治疗老年性慢性腹泻可显著缓解临床症状,调控胃肠激素及脑肠肽水平治疗效果确切。     

定点旋转复位法联合雷火灸治疗腰椎间盘突出患者的临床疗效及其对患者脊柱稳定性的影响〖JZ)〗〖HS)〗

目的:探讨定点旋转复位法联合雷火灸治疗腰椎间盘突出患者的临床疗效及其对患者脊柱稳定性的影响。方法:选取2017年5月至2018年9月梧州市妇幼保健院收治的腰椎间盘突出患者100例作为研究对象,按照随机数字表法分为对照组和观察组,每组50例。对照组给予定点旋转复位法治疗;观察组在对照组的基础上联合雷火灸治疗,2组均治疗2周。治疗后统计2组临床疗效;比较2组治疗前后社会活动功能、脊柱稳定情况、腰痛缓解情况及直腿抬高角度。结果:治疗后观察组临床总有效率为88. 00%,显著高于对照组的72. 00%,差异有统计学意义(P 0. 05);与治疗前比较,治疗后2组患者腰椎曲度、腰椎前凸角度、腰骨倾斜角、平均椎间隙高度、日本骨科学会腰痛评分(JOA)及直腿抬高角度(ASLR)显著升高,且观察组显著高于对照组,差异有统计学意义(P 0. 05),2组Oswestry功能障碍指数(ODI)及视觉模拟评分法(VAS)评分显著降低,且观察组显著低于对照组,差异有统计学意义(P 0. 05)。结论:定点旋转复位法联合雷火灸可显著缓解腰间盘突出症患者疼痛及下腰痛症状,提高其社会活动功能、直抬腿高度及脊柱稳定性,疗效显著优于单独定点旋转复位法治疗。     

用于精神分裂症检测的眼动仪研制

目的为了辅助医生诊断精神分裂症患者,设计一种高精度桌面式眼动仪。方法系统采用双屏输出,摄像机集成了红外光源及红外滤光镜片。重点提出一种新的瞳孔中心识别算法,首先对预处理后的眼图进行瞳孔轮廓提取,然后采用最小二乘椭圆拟合法识别瞳孔中心。在此基础上,对瞳孔周围区域进行边缘检测,得到的最大轮廓中心即为普尔钦光斑中心。最后基于非线性二阶多项式拟合静态标定算法建立了眼图坐标系和场景坐标系的映射方程,并完成了注视点标定。结果实现了眼动轨迹的实时显示和凝视点数目的统计,测试有效率达到80%以上。结论系统能够很好地跟踪和显示人眼运动,具有较好的实用性。     

Was acupuncture developed by Han Dynasty Chinese anatomists?

Anatomical dissection has begun to reveal striking similarities between gross anatomical structures and the system of nomenclature used in traditional Chinese acupuncture. This paper argues that acupuncture point nomenclature is rooted in systematic anatomical investigation of cadaveric specimens, and that acupuncture points and meridians are purposefully named to reflect observable physical form. Two types of evidence are compared: observations of physical structures based on anatomical dissection, and translation and analysis of original Chinese texts. Evidence is contextualized through in‐depth practical understanding of acupuncture. Points designated as 天 tian (heavenly/superior), 下 xia (below/inferior), 髎 liao (bone‐hole), 飛 fei (flying), 委 wei (bend), and 谿 xi (mountain stream/ravine) are investigated. These acupuncture point names: (a) specify position; (b) reflect function and/or form; (c) indicate homologous structures; (d) mark unusual structures; and/or (e) describe the physical appearance of a deep (dissected) structure by likening it to a homologous everyday object. Results raise intriguing possibilities for developing an understanding of acupuncture points and meridians firmly based in the material and functional anatomy of the human body. Such an understanding has the potential to open new fields of thought about functional anatomy. It also has implications for future investigations into the mechanisms of acupuncture, and gives some insights into the possible origins of this iconic area of Chinese medicine. Anat Rec, 299:643–659, 2016. © 2016 Wiley Periodicals, Inc.