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991.
992.
The Inhibitory Effect of Radix Salviae Miltiorrhizae on Hypoxic Structural Remodeling of Intra-acinarPulmonary Arteries 总被引:1,自引:0,他引:1
(席思川)(车东媛)(张婉蓉)TheInhibitoryEffectofRadixSalviaeMiltiorrhizaeonHypoxicStructuralRemodelingofIntra-acinarPulmonaryArteries¥XISi-c... 相似文献
993.
Identification and characterization of a DR4-restricted T cell epitope within chlamydia heat shock protein 60 总被引:11,自引:0,他引:11 下载免费PDF全文
K H O DEANE R M JECOCK J H PEARCE J S H GASTON 《Clinical and experimental immunology》1997,109(3):439-445
An epitope within the 60 kD Chlamydia trachomatis heat shock protein (hsp) 60, recognized by a HLA-DRB1*0401-restricted T cell clone from a reactive arthritis patient, has been characterized. Stimulatory peptides contained a nine amino acid sequence (residues 38–46) predicted by algorithm to confer strong binding to DRB1*0401, with valine in the P1 position. The overall length of the peptide was critical for efficient recognition; peptides with at least one residue N-terminal to the putative P1 position were markedly more stimulatory than a peptide whose N-terminal is the P1 valine. Optimal responses were seen with 14mer peptides having two to three amino acids N- and C-terminal to the core 9mer. The sequence of the defined epitope is identical in hsp60 from both C. trachomatis and C. pneumoniae. Since the latter is a common respiratory pathogen, patients infected with C. trachomatis may already be primed for responses to hsp60 by prior infection with C. pneumoniae. Such secondary responses are important in the pathogenesis of chlamydia-induced inflammatory diseases such as trachoma. Priming by infection with enteric organisms was considered because of the similarity of the epitope sequence in Escherichia coli hsp60. However, although an E. coli-related peptide was recognized, intact E. coli hsp60 was not, suggesting that the epitope is cryptic in E. coli hsp60. Human hsp60 has six amino acid differences from chlamydial hsp60 in the epitope sequence and was not recognized. Thus cross-reactive recognition of self hsp60 could not be implicated in the pathogenesis of chlamydia-induced reactive arthritis in this patient. 相似文献
994.
Kazuhito Yamamoto Hirotaka Osada Masao Seto Michinori Ogura Hisamitsu Suzuki Kazuhiko R. Utsumi Atsushi Oyama Yutaka Ariyoshi Shigeo Nakamura Souji Kurita Toshitada Takahashi Ryuzo Ueda 《Cancer science》1992,83(5):465-476
A case of non-Hodgkin's lymphoma showed a phenotypic and genotypic cell lineage switch twice during nine years of his clinical history; first, T-cell type, pleomorphic small cell lymphoma developed, followed by B-cell type, diffuse centroblastic/centrocytic lymphoma, and finally T-zone lymphoma without follicles again developed, from which AST-1 cultured cell line was established. Karyotype analysis demonstrated a shared abnormal chromosome, der(1)t(1;?)(p36;?), among the first relapsed B-cell tumor, the second relapsed T-cell tumor and AST-1 cell line. Furthermore, T-cell receptor (TCR) γ gene rearrangement bands of the same size were observed in the first relapsed B-cell tumor and the second relapsed T-cell tumor as well as AST-1 cell line. These results suggested that both relapsed tumors of different cell lineages are derived from a common malignant clone, presumably a committed lymphoid stem cell. A unique translocation, t(2;14)(q37;q11.2), which may involve TCR δ/α gene complex, was observed in the second relapsed tumor and AST-1 cells. To attempt to isolate the breakpoint of this translocation, the configuration of TCR δ/α gene complex was studied. The result showed that two rearrangements of TCR α gene detected with Jα probes were the products of the normal TCR rearrangement process, and were not involved in the translocation at this region. This patient, together with the AST-1 cell line, provided us a unique opportunity to study the development and clonal evolution of malignant lymphoma. 相似文献
995.
目的:研究雷公藤内酯醇对体外培养的牛晶状体上皮细胞增殖的抑制作用。方法:取第4代对数生长期的牛晶状体上皮细胞培养24h后,加入重组人表皮生长因子(终浓度50μg/L)和不同浓度的雷公藤内酯醇(40,20,10μg/L),分别作用6,12,24,48,72h后,采用MTT法观察雷公藤内酯醇对牛晶状体上皮细胞增殖的影响。结果:不同浓度的雷公藤内酯醇,于不同时间作用在处于增殖状态的LECs,其增殖抑制率升高呈现明显的时间及剂量依赖性。结论:雷公藤内酯醇对晶状体上皮细胞增殖有明显的抑制作用在防止后囊混浊方面有广阔的前景。 相似文献
996.
目的:对涎腺腺样囊性癌(ACC)中增殖细胞核抗原(PCNA)和P53蛋白的表达及分布特点进行分析。方法:采用免疫组织化学ABC方法对36例ACC进行了研究。ACC按病理形态分为筛孔型、腺管型和实体型。结果:PCNA阳性表达率为77.78%,其中筛孔型69.23%,腺管型78.95%,实体型为100%。P53蛋白表达的阳性率为36.11%,其中筛孔型为30.77%,腺管型为26.32%,实体型为100%。结论:PCNA表达指数与染色强度可作为评价腺样囊性癌恶性程度的生物学指标,P53基因突变可能在腺样囊性癌的发生发展中具有一定作用,其对癌细胞的增殖具有促进作用。 相似文献
997.
癌变的早期诊断对提高疗效具有特殊意义,为了寻求简捷,高效,超前,可靠的诊断方法,实验以DMBA(二甲基苯并蒽)诱导的金地鼠颊囊致癌模型为对象,以PCNA(增殖细胞核抗原)BrdV(溴脱氧尿嘧啶)等免疫组化技术为手段;以光镜观察诊断为对照,对三种观察结果进行相关分析,发现三者间有高度显著的相关性,证实了免疫组化方法在癌变诊断中有一定的参考价值,实验还发现PCNA和BrdU检测结果比较每天敏感,简捷, 相似文献
998.
Anieta M. Sieuwerts Jan G. M. Klijn John A. Foekens 《Clinical & experimental metastasis》1997,15(1):53-62
The Boyden chamber assay is widely used for in vitro measurement of the invasive capacity of cells. However, results can be affected significantly if certain precautions are not taken. Using the Boyden chamber assay we investigated in vitro the invasive potential of a variety of human gynecological tumor cell lines to degrade and migrate through the artificial basement membrane matrix Matrigel. However, in the absence of this Matrigel layer large differences were observed in the ability of cells to adhere to, migrate through and attach to the lower side of the filter membranes. These differences were influenced by cell density, degree of directional locomotion, and the size of the filter pores. To adjust for these influences (which are not directly correlated to the capacity of cells to traverse the Matrigel layer), invasion results were corrected for the ability of cells to migrate through the filter membrane. In addition, the invasion of MDA-MB-231 cells was used as an internal standard to compensate for variations in the Matrigel layer between different experiments. Overall, in our experimental set up, the five human breast cancer cell lines were the most invasive (mean invasion ± SEM relative to MDA-MB-231 invasion: 104.7 ± 6.1%), the five human ovarian cancer cell lines the least invasive (60.2 ± 2.2%) and the six human endometrial cancer cell lines showed an intermediate capacity (79.1 ± 3.5%). In conclusion, the Boyden chamber assay can be used reliably for studying the invasive potential of cells in vitro, if the ability of the cells to migrate through the filter is taken into account, and a reference cell line is included to enable comparison of the data obtained from independently performed experiments on different cell lines. 相似文献
999.
《Pathology international》1997,47(11):809-811
The Association of Directors of Anatomic and Surgical Pathology have developed recommendations for the surgical pathology reporting of common malignant tumors. The recommendations for carcinoma of the larynx are reported herein. 相似文献
1000.
人重组C3片段通过IL-2自分泌效应对CTLL-2细胞产生促增殖作用 总被引:1,自引:0,他引:1
目的研究补体C3片段的体外生物学活性及其作用机理,进一步探讨C3片段与免疫细胞的关联。方法利用重组DNA技术表达纯化C3活性片段(命名为C33),观察其对IL-2依赖性的小鼠杀伤性T细胞株CTLL-2细胞的促增殖效应,并通过抗体封闭途径和分子杂交技术研究C33作用的机理。结果发现C33蛋白对CTLL-2细胞具有明显的促增殖作用,并呈剂量依赖关系;这一作用能够部分地被抗小鼠CD11b抗体所封闭,能够完全被抗小鼠IL-2抗体所封闭;分子杂交显示C33蛋白能够明显刺激CTLL-2细胞的IL-2mRNA表达。结论人重组C3片段C33可通过IL-2的自分泌效应对CTLL细胞产生促增殖作用,补体受体CR3参与这一作用。 相似文献