小鼠肝枯否细胞的分离、培养与鉴定

目的研究BALB／c鼠肝枯否细胞分离、培养和鉴定。方法选用健康雌性8—10w龄鼠，麻醉无菌取肝，D—Hank’s液注射冲洗去血并剪去部分结缔组织，剪碎肝组织，加入链霉蛋白酶E和Ⅰ型胶原酶，水浴振荡消化，再加入DNase Ⅰ，共同消化。不锈钢筛网滤过，用0．005％DNase Ⅰ的1640液清洗细胞液，加Tris—NH4CL溶解红细胞。50％-70％Percoll梯度离心，用含10％FCS的RPMI-1640贴壁培养4—6h洗去非贴壁细胞。通过吞噬墨水实验鉴定枯否细胞。结果枯否细胞的数量为（1．07±0．87）×10^7／g，贴壁率40．3％，用0．4％台盼蓝染色鉴定，细胞存活率在95％以上，吞噬实验发现90％的贴壁细胞具有吞噬功能，即KC的纯度可达90％。贴壁的枯否细胞的形态多样，已经完全伸出了伪足并且伸展贴壁，呈多边形、多角形或者梭形等不规则形状，并且胞浆内可见吞噬的微粒状物质。结论酶消化水浴振荡法结合梯度离心和贴壁培养是分离BALB／c鼠枯否细胞的可靠方法，为进一步实验打下了基础。     

来华医学学历留学生教育发展趋势初探

针对近年我国来华医学学历留学生教育持续发展现状，对周边国家医学教育需求、不同培养模式优劣以及培养质量标准化等进行了分析，探讨了来华医学学历留学生教育未来发展趋势。     

基于分流教学的课程管理研究

分流教学是近年我校教学工作改革的重要措施，分流的目的是以全面提高教学质量为宗旨，遵循“有教无类”、“因材施教”的教学原则，解决我校学生多层次学习基础的教学矛盾。本文以课程为切入点，分析了目前分流教学存在的问题，对分流教学改革提出了对策性建议。     

医院临床药师的在职培养

OBJECTIVE Combine to the situation of the clinical pharmacy work of the hospital,to study the current situation that clinical pharmacist talents lack,accelerate way and method that a clinical pharmacist trains.METHODS According to the current situation th     

适应公共卫生事业发展培养预防医学实用性人才

目前我国的公共卫生队伍专业人才缺乏,医学模式的转变和公共卫生体系的建设给预防医学教育提出了更高的要求,高等院校的预防医学教育正在积极探索预防医学教学改革,针对公共卫生体制的改革,积极调整课程设置和修改培养计划,加强人文社科知识和管理学知识的教学,强调能力的培养,采取请进来、走出去的办法,加强理论与实践的结合,培养预防医学的使用性人才。     

濒危野生药材岩黄连人工栽培技术

岩黄连Corydalis saxicolaBunting为石山地区特有的多年生草本药用植物,在桂西北地区常用于消炎止痛、拔毒、治疗疥疮中毒,亦是治疗乙型肝炎、肝硬化、肝癌等疾病的中草药。目前,岩黄连的野生资源日渐减少,已无法满足生产之急需。文章总结了作者十多年来对岩黄连进行引种驯化与人工栽培繁殖试验的研究成果,对岩黄连的生态生物学特性以及种植栽培、田间管理、病虫害防治、采收加工等种植栽培过程中的关键技术进行了总结,为开展岩黄连的中药材生产质量管理规范(GAP)种植提供了重要的参考。     

江西彭泽贝母栽培技术研究

论述了江西彭泽贝母的生态环境、生长习性、物候期以及栽培技术。     

建立检验专业人才培养的教学质量监控体系

本文阐述了重庆医科大学医学检验系近年来根据人才培养目标,在强化教育管理,建立全方位、多层次的教学质量监控体系,确保医学检验专业人才培养质量方面所作的研究工作。     

Prenatal diagnosis of Gaucher disease Assay of the ß-glucosidase activity in amniotic fluid cells cultivated in two laboratories with different cultivation conditions

Sixteen pregnancies at risk for Gaucher disease - six with the Norrbottnian form, one with a juvenile form with a similar clinical course to the patients from Norrbotten and nine with the infantile form - have been monitored by the assay of β-glucosidase activity in cultivated amniotic fluid cells with natural labelled glycosylceramide as substrate. Two methods of cultivation were compared in respect of their effect on the activity of lysosomal enzymes. No significant difference was found between the two marker enzymes, β-galactosidase and N -acetyl-β-glucosaminidase, but the β-glucosidase activity was significantly higher in the cells cultivated with one of the methods. In four of the pregnancies at risk, the β-glucosidase activity in the cultivated amniotic fluid cells was less than 5 % of that in the two control materials. These fetuses were regarded as affected with Gaucher disease and were aborted. Differentiation between controls and Gaucher heterozygotes was not possible in cultivated amniotic fluid cells. The diagnosis of Gaucher disease in the amniotic fluid cells was confirmed in three of the four cases by the assay of the β-glucosidase activity in the liver and brain of the aborted fetuses. The glucosylceramide content of the liver from two aborted fetuses was not augmented. The β-glucosidase activity was examined in seven placentas from pregnancies at risk for Gaucher disease and found to be in agreement with that in the cultivated amniotic fluid cells.     

优化幽门螺杆菌最佳培养保存条件实验研究

目的:研究不同培养基对幽门螺杆菌(Hp)生长的影响;优化Hp培养保存条件;方法:采用正交设计的方法;结果:Hp最佳固体培养条件为92%哥伦比亚琼脂或脑心浸液琼脂加5%牛血清、再加2.5%混合抗生素,pH值为7.5.采用脑心浸液加甘油制成的冷冻保存液置于-70℃低温条件下保存,Hp菌株激活复苏培养成功率最高.结论:不同培养条件下Hp生长情况存在显著差异.