白藜芦醇苷对大鼠局灶性脑缺血的保护作用

目的 研究白藜芦醇苷(PD)对大鼠局灶性脑缺血的保护机制。方法 采用改良的线栓法制作大鼠大脑中动脉栓塞模型(MCAO)。将健康成年大鼠随机分为3组：假手术组、缺血模型组、缺血前PD处理组。测定脑组织含水量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—Px)、过氧化氢酶(CAT)及一氧化氮合酶(NOS)活性，并进行病理组织学检查。结果 PD能明显提高缺血脑组织中SOD、GSH—Px、CAT活性，降低MDA含量，减轻脑水肿，并有降低缺血脑组织中NOS活性的趋势，病理组织学检查显示，PD能改善脑缺血造成的大鼠脑组织损伤。结论 PD对大鼠局灶性脑缺血有明显的保护作用，其机制可能是通过有效的拮抗自由基损伤，提高脑组织抗氧化能力来实现的。     

虎杖苷对THP-1细胞增殖及凋亡的影响及其作用机制研究北大核心CSCD

目的探讨虎杖苷对急性单核细胞白血病细胞株THP-1增殖及凋亡的影响及可能的作用机制。方法以不同梯度浓度的虎杖苷处理THP-1细胞24 h、48 h,CCK-8法检测细胞增殖活力,并计算半数抑制浓度。取对数生长期的THP-1细胞,分为虎杖苷处理组(处理浓度为半数抑制浓度)和空白对照组(细胞中未施加虎杖苷溶液处理),培养48 h后,采用流式细胞术检测细胞凋亡及细胞周期;Western blot法检测PI3K、AKT、p-AKT、mTOR、p-mTOR、p70 S6K、p-p70 S6K蛋白的表达。结果虎杖苷可有效抑制THP-1细胞的增殖,48 h的半数抑制浓度为1800μmol/L。经1800μmol/L的虎杖苷溶液作用48 h后,THP-1细胞的凋亡率较空白对照组显著增加(P<0.05);细胞周期出现G0/G1期至S期的阻滞,表现为G0/G1期的细胞比例较空白对照组明显上升,S期的细胞比例较空白对照组显著下降(P<0.05);PI3K、AKT、p-AKT、mTOR、p-mTOR、p70 S6K、p-p70 S6K蛋白表达较空白对照组显著降低(P<0.05)。结论虎杖苷可有效抑制THP-1细胞的增殖,阻滞细胞周期并诱导细胞凋亡,其作用机制可能与PI3K/AKT/mTOR信号通路的抑制表达有关。     

甲酰化肽样受体介导脂多糖诱导的白细胞趋化反应及虎杖甙的调节作用研究

目的：初步研究虎杖甙（PD）对脂多糖刺激下白细胞趋化活性调节的机制。 方法： 用趋化小室方法测定虎杖甙对LPS刺激下多形核白细胞（PMN）趋化活性及转染了甲酰化肽样受体（FPRL1）基因的293细胞（FPRL1/293）趋化活性的影响。 结果： LPS及LPS刺激的PMN上清可增强多形核白细胞的趋化反应；同样LPS刺激下转染了甲酰化肽样受体基因的293细胞的趋化性显著升高。而PD可下调LPS刺激引起的PMN和FPRL1/293细胞的趋化性升高。 结论： 甲酰化肽样受体FPRL1可能参与介导脂多糖引起的白细胞趋化反应，PD可能通过抑制白细胞趋化因子的分泌和下调甲酰化肽样受体活性来调节细胞的趋化性。     

虎杖甙对正常大鼠血管平滑肌细胞内游离钙浓度的影响

目的：观察虎杖甙（ＰＤ）对培养的大鼠血管平滑肌细胞（ＶＳＭＣ）内钙离子浓度的变化机理。方法：用Ｆｌｕｏ－３－ＡＭ标记培养的ＶＳＭＣ，在粘附式细胞仪上测定细胞内游离钙的变化。结果：实验结果表明，ＰＤ（００２～２０ｍｍｏｌ／Ｌ）可使大鼠ＶＳＭＣ内游离钙浓度升高，波形变宽，其引起的钙波形态与去甲肾上腺素（ＮＥ）及氯化钾所引起的高尖钙波不同，ＰＤ使ＶＳＭＣ产生一种剂量依赖性的、持续缓慢升高的钙波。ＰＤ引起的细胞内游离钙升高可被ＥＧＴＡ（２ｍｍｏｌ／Ｌ）及维拉帕米（５０μｍｏｌ／Ｌ）明显抑制。结论：以上结果提示ＰＤ既促进ＶＳＭＣ外钙离子进入细胞内，还能诱导细胞内钙离子释放；ＰＤ可能会提高正常ＶＳＭＣ的收缩性，增加正常血管的张力。     

虎杖苷抗肺缺血再灌注损伤作用及对磷酸化蛋白激酶Cα调节的实验研究

目的：探讨虎杖苷（PD）抗肺缺血再灌注损伤作用及对磷酸化蛋白激酶Cα（PKCα）活性调节。方法:采用在体单肺原位缺血再灌注损伤模型。健康日本大耳白兔40只随机均分成假手术对照组（sham组）、肺缺血再灌注组(I/R组)、肺缺血再灌注+虎杖苷组（PD组）、肺缺血再灌注+PD+多粘菌素B组（PMB组）。各组在不同时点抽血检测丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性。实验末，测湿干重比（W/D），计算肺泡损伤率(IAR)，电镜观察细胞超微结构改变，免疫组化分析磷酸化PKCα表达情况。结果:①I/R组和PMB组血清SOD活性降低无明显差异，PD组显著高于I/R组（P<0.01）；而MDA的上升PD组显著慢于I/R组、PMB组（P<0.05或P<0.01）。② PD组的W/D与IAR均显著低于IR组和PMB组（均P<0.01）。 ③ 电镜提示PD组损伤程度明显轻于IR组及PMB组。④除I/R组外，免疫组化显示磷酸化PKCα皆阴性表达。结论:PD对LIRI具有防治作用，其机制除抗氧化损伤外可能还与抑制PKCα活化有关。     

Effects of polydatin from Polygonum cuspidatum on lipid profile in hyperlipidemic rabbits

Hyperlipidemia is one of the vital coronary risk factors and is positively related to morbidity and mortality of coronary heart disease. There are numerous herbal medicines which are reported to exert good hypolipidemic actions with few side effects.     

双波长HPLC法同时测定妇科Ⅳ颗粒剂中芍药苷和虎杖苷的含量

目的：建立妇科Ⅳ颗粒剂中芍药苷和虎杖苷含量的HPLC测定方法。方法：采用Phenomenex Luna C18（4.6 mm×250 mm,5μm）色谱柱;流动相为乙腈-0.1%磷酸（17∶83）;流速为1.0 mL·min-1;柱温为30℃;检测波长为230 nm、306 nm。结果：芍药苷在0.524-8.384μg、虎杖苷在0.344-5.502μg范围内,进样量与峰面积具有良好线性关系（r=0.9997,r=0.9999）,平均回收率分别为99.6%（RSD=0.92%）、100.3%（RSD=1.32%）。结论：研究建立的含量测定方法可靠、准确、重现性好,可用于该制剂的质量控制。     

虎杖4号对兔血小板超微结构的影响

通过透射电镜观察兔血小板经虎杖晶4号作用后的超微结构变化。为探讨虎杖4号（PD）抑制血小板变形，释放反应机理提供形态学依据。取6只新西兰克，心脏穿刺取血制备富含血小板血浆（PRP），将PRP分为正常对照，生理盐水、吲哚美辛组及3种不同浓度PD实验组。除正常对照组外其余5组均用花生四烯酸（AA）诱导血小板聚集，按超薄切片常规制样，电镜观察各组血小板的超微结构变化，结果证明PD不仅对血小板的变形反应和释放反应有明显的抑制作用而且存在着量效关系。     

Polydatin loaded collagen/heparin sulfate scaffold in the treatment of spinal cord injury in rats北大核心

BACKGROUND: At present, the research on the neuroprotective effect of polydatin has gradually become a hot spot in the field of neurology. The research direction is mostly focused on ischemic cerebrovascular disease, and there are few studies on the application of spinal cord injury. OBJECTIVE: With collagen/heparin sulfate scaffold as carrier, polydatin was applied to the injured spinal cord to observe the repair effect. METHODS: (1) Collagen/heparin sulfate scaffolds and collagen/heparin sulfate scaffolds loaded with 0.5, 1, 1.5 mmol/L polydatin were prepared. The third generation of rat neural stem cells was seeded on four kinds of scaffolds, and the proliferation of cells was detected by CCK-8 assay. During inducing neural differentiation of neural stem cells, the expression levels of glial fibrillary acidic protein, Tuj-1 and Oligo were detected by immunofluorescence staining and RTPCR. (2) The spinal cord injury model of adult male SD rats was established and divided into three groups. The model control group (n=10) was not implanted with any materials. The control group (n=10) was implanted with collagen/heparin sulfate scaffold, and the experimental group (n=10) was implanted with 1 mmol/L polydatin/collagen/heparin sulfate scaffold. Meanwhile, sham operation group (n=10) was set up. BBB score was used to test the motor function of the right hind limb within 8 weeks after operation. At 8 weeks after operation, the spinal cord tissues were taken for histological observation, immunohistochemical analysis, and western blot assay. RESULTS AND CONCLUSION: (1) At 3 and 7 days of culture, the absorbance value of cell proliferation on 1 mmol/L polydatin/collagen/heparin sulfate scaffold was higher than that on the other three scaffolds (P < 0.05). (2) Immunofluorescence staining 7 days after induction showed that the expression of glial fibrillary acidic protein in 1 and 1.5 mmol/L polydatin/collagen/heparin sulfate scaffold groups were less than those in the other two collagen/heparin sulfate scaffold groups, but the expression levels of Oligo and Tuj-1 were more than those in the other two collagen/heparin sulfate scaffold groups. (3) RT-PCR results showed that the expression of glial fibrillary acidic protein mRNA in 1 and 1.5 mmol/L polydatin/collagen/heparin sulfate scaffold groups was lower than that in the other two collagen/heparin sulfate scaffold groups (P < 0.05). There was no significant difference in the expression of Tuj-1 mRNA among the four groups. The expression of Oligo mRNA in 1 mmol/L polydatin/collagen/heparin sulfate scaffold group was higher than that in the other three groups (P < 0.05). (4) Spinal cord injury repair experiments showed that the BBB score of the experimental group was always higher than that of the model control group and the control group 2-8 weeks after operation (P < 0.05). Hematoxylin-eosin staining showed that the spinal cord defects in the control group and the observation group were filled with scaffolds, and the space between the tissues was smaller than that in the model control group. The tissue continuity and space in the observation group were better than those in the control group. Immunohistochemical analysis and western blot assay showed that the expression of neurofilament-200 protein in the experimental group was higher than that in the control group and model control group (P < 0.05). The expression of glial fibrillary acidic protein in the experimental group was lower than that in the control group and model control group (P < 0.05). (5) It is concluded that polydatin loaded collagen/heparin sulfate scaffolds can promote the repair of spinal cord injury. © 2022, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.