Iodine-125 labeled phenylphosphonic acid: A new radiopharmaceutical for long-term investigations of the skeleton

A skeletal seeking radiopharmaceutical labeled with a long-lived radionuclide was developed to evaluate regional bone formation and its subsequent resorption. The agent is [phosphonate (phenylmethylene hydroxy) bis]-I-125 or I-125 PA. Tissue distribution studies in mice (N=16) showed approximately 40% of the administered dose to be retained by the skeleton up to 336 hours post IV injection. The percentage of the dose accumulated by the thyroid gland remained at less than 0.5%, indicating minimal deiodination of the I-125 PA. Whole body retention studies in the same species revealed a triexponential release pattern with the longest component comprising 33% of the dose with a biologic half-life of 962 days. A fractured rat tibia model was studied with I-125 PA and Tc-99^m MDP. Chronic loss of the I-125 PA relative to normal tibia was quantitated: five days (62.8%); 30 days (47.4%). Concomitant increased uptake of the Tc-99^m MDP was observed at the fracture site relative to normal: five days (186%); 30 days (1,041%). The above data suggest that I-125 PA can be utilized to measure acute bone formation and chronic resorption.     

对子宫腺肌病患者血清CA125及EMAb的临床评价

目的：探讨血清CA125及子宫内膜抗体（EMAb)测定，对子宫腺肌病的诊断及疗效评估的临床价值。方法：经术后病理学检查确诊子宫腺肌病患者63例和子宫肌瘤患者45例，均于术前及术后测定两组血清CA125水平和EMAb。结果：子宫腺肌病组术前血清CA125平均水平及EMAb阳性率均高于子宫肌瘤组，两者相比差异有显著性（P＜0．001及P＜0．01）。联合测定血清CA125水平和EMAb，以两者均阳性为诊断标准，诊断子宫腺肌病的敏感性为54．63％，特异性为100％。子宫腺肌病组术后血清CA125水平和EMAb阳性率均较术前下降，差异有显著性（P＜0．01及P＜0．05）。结论：测定血清CA125及EMAb对子宫腺肌病有较好的辅助诊断价值，联合检测更能提高诊断的正确性，并可作为评价子宫腺肌病疗效的敏感指标。     

血清CA125检测在子宫内膜癌中的价值

目的探讨血清CA125在子宫内膜癌中的价值.方法选取1992年3月～2002年3月在北京大学第一附属医院、北京大学人民医院住院经手术治疗的子宫内膜癌患者141例,术前及随访中用放射免疫法测定血清CA125水平,CA125≥35 U/ml 为阳性结果.对其中14例行子宫内膜癌组织CA125免疫组化方法检测.收集患者的临床病理资料,分析CA125与这些资料的关系以及复发患者复发前后CA125变化.结果 CA125免疫组化检测14例均呈阳性,阳性细胞着色率与血清CA125之间无明显相关.141例患者术前血清CA125阳性32例(22.7%), Ⅰ、Ⅱ、Ⅲ和Ⅳ期患者血清CA125阳性(阳性率)分别为11例(12.1%)、6例(31.6%)、12例(46.2%)和3例(60.0%). 血清CA125阳性率随子宫内膜癌期别的增加而升高.深肌层浸润、宫颈受累、附件转移、腹腔洗液细胞学阳性、盆腔淋巴结转移及宫内肿瘤病灶≥2 cm者,血清CA125阳性率增加.随访中13例复发,其中9例术前血清CA125＞35 U/ml者复发时均伴血清CA125水平升高,而另4例术前血清CA125正常者,复发后血清CA125水平仍正常.结论子宫内膜癌患者术前血清CA125的测量有助于了解肿瘤的侵犯范围.术前血清CA125水平异常的子宫内膜癌患者,术后定期复查血清CA125水平,将有助于子宫内膜癌病情的监测.     

南瓜子的研究进展

本文对近年来有关南瓜子的化学成分、药理作用、临床应用等方面的研究进展作一概述。其化学成分主要含有脂肪酸，植物甾醇，氨基酸，维生素和矿物质等。药理研究表明有驱除寄生虫，降低LDL胆固醇，抗炎，缓解高血压，减少膀胱和尿道张力等作用，毒性很小。临床报道用于治疗绦虫病，血吸虫病，前列腺炎，前列腺增生，改善膀胱刺激征，防止尿道结石形成等。     

[125I], [127I]‐and [14C]‐Labelling of the GLP‐1‐(7‐37) derivative NN2211

Arg³⁴Lys²⁶(N^ε‐(γ‐L ‐glutamyl(N^α‐palmitoyl)))‐GLP‐1(7‐37) (NN2211) is currently in development as a diabetes type 2 drug. The fatty acid attached to the GLP‐1(7‐37) ensures a long and controlled duration of action. The synthesis of [¹²⁵I]NN2211, [¹²⁷I]NN2211 and [¹⁴C]NN2211 used for preclinical ADME studies are described. NN2211 was iodinated using the lactoperoxidase/hydrogen peroxide method, and [¹⁴C]NN2211 was synthesized in 4 steps by two routes both starting from an α‐protected [U‐¹⁴C]glutamic acid. Copyright © 2003 John Wiley & Sons, Ltd.     

A napin‐like polypeptide with translation‐inhibitory,trypsin‐inhibitory,antiproliferative and antibacterial activities from kale seeds

Abstract: A heterodimeric napin‐like polypeptide with translation‐inhibiting and antibacterial activities has been isolated from kale seeds. The purification procedure entailed ion‐exchange chromatography on dielthylaminoethyl (DEAE)‐cellulose, affinity chromatography on Affi‐gel blue gel, ion‐exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on Superdex 75. The napin‐like polypeptide was unadsorbed on DEAE‐cellulose but adsorbed on Affi‐gel blue gel and Mono S. Its 7‐kDa large subunit differs in N‐terminal amino acid sequence from the 4‐kDa small subunit. The polypeptide inhibited translation in the rabbit reticulocyte lysate system with an IC₅₀ of 37.5 nm . This activity was preserved between pH 5 and pH 11, and between 10 and 40 °C. It fell to a low level at pH 3 and pH 13 and at 70 °C. Antibacterial activity against Bacillus, Megabacterium, and Pseudomonas species and antiproliferative activity against leukemia L1210 cells were observed. However, the polypeptide did not exert antifungal, ribonuclease, or protease activity.     

CO2激光对大叶龙胆种子萌发及幼苗生长的影响

目的 研究CO2激光对大叶龙胆种子萌发及幼苗生长的影响。方法 使用不同剂量的CO2激光辐照大叶龙胆湿种子，观察不同光照条件下种子的萌发情况，并研究激光处理后的幼苗生长情况。结果 光照条件下．激光可提高大叶龙胆种子的发芽势及发芽率，但对黑暗条件培养的种子无明显促萌发作用。低剂量激光还可提高大叶龙胆幼苗的叶绿素和可溶性蛋白含量，促进幼苗生长。结论 低剂量的CO2激光可促进光照条件下的大叶龙胆种子萌发及幼苗生长。     

手术中种植放射碘-125微粒近距离放疗在低位直肠癌综合治疗中的应用

 目的　探讨组织间种植放射碘 12 5 (12 5I)微粒近距离放疗在低位直肠癌综合治疗中的应用。方法　可手术低位直肠癌 82例 ,肿瘤距肛缘在 4～ 6cm者分为盆腔自主神经保留 (PANP)加肛门重建术(ARO)加12 5I微粒种植组 (实验组 :A组 )和Miles手术组 (对照组 :B组 ) ;肿瘤距肛缘 6～ 8cm者分为超低位前切除 (ASP)加PANP加12 5I微粒种植组 (实验组 :C组 )和ASP组 (对照组 :D组 )。随访比较各组间局部复发率、5年生存率、排便功能和性功能障碍等。结果　A与B组及C与D组间局部复发率、控便功能、排尿和性功能障碍发生率、5年生存率差异有统计学意义 (P <0 .0 5 ) ,A、C两组术后局部复发率、排尿功能和性功能障碍发生率分别低于对照组B、D组 (P <0 .0 1)。而术后吻合口瘘、吻合口狭窄和骨盆腔感染的发生率差异无统计学意义。结论　组织间永久性种植12 5I微粒近距离放疗疗效可靠 ,可降低Miles手术的比例 ,有效预防功能保存手术后的局部复发 ,提高 5年生存率和病人的生存质量。     

卵巢癌患者TPS与CA125测定

[目的]探讨卵巢上皮性癌患者组织多肽特异抗原(tissue polypeptide specificantigen,TPS)与CA125水平测定及其临床意义.[方法]采用ELISA法和EIA法分别检测96例卵巢上皮性癌患者和33例妇科良性疾病患者血清TPS与CA125水平并进行比较分析.[结果]Ⅲ Ⅳ期卵巢上皮性癌患者血清TPS和CA125平均水平及阳性率均高于Ⅰ Ⅱ期(P<0.05),治疗有效组TPS和CA125水平显著低于初诊未治组,而复发转移组TPS水平明显高于初诊未治组.[结论]TPS与CA125水平的联合检测对卵巢癌诊断、疗效及预后有临床应用价值.