收费全文 | 27826篇 |
免费 | 1414篇 |
国内免费 | 344篇 |
耳鼻咽喉 | 389篇 |
儿科学 | 546篇 |
妇产科学 | 338篇 |
基础医学 | 1889篇 |
口腔科学 | 1244篇 |
临床医学 | 4166篇 |
内科学 | 1671篇 |
皮肤病学 | 162篇 |
神经病学 | 4319篇 |
特种医学 | 1862篇 |
外国民族医学 | 1篇 |
外科学 | 4181篇 |
综合类 | 3186篇 |
一般理论 | 3篇 |
预防医学 | 1534篇 |
眼科学 | 874篇 |
药学 | 1690篇 |
34篇 | |
中国医学 | 288篇 |
肿瘤学 | 1207篇 |
2024年 | 28篇 |
2023年 | 409篇 |
2022年 | 554篇 |
2021年 | 1048篇 |
2020年 | 995篇 |
2019年 | 849篇 |
2018年 | 908篇 |
2017年 | 969篇 |
2016年 | 870篇 |
2015年 | 818篇 |
2014年 | 1775篇 |
2013年 | 2144篇 |
2012年 | 1318篇 |
2011年 | 1628篇 |
2010年 | 1250篇 |
2009年 | 1422篇 |
2008年 | 1397篇 |
2007年 | 1357篇 |
2006年 | 1288篇 |
2005年 | 1106篇 |
2004年 | 936篇 |
2003年 | 762篇 |
2002年 | 620篇 |
2001年 | 582篇 |
2000年 | 455篇 |
1999年 | 441篇 |
1998年 | 368篇 |
1997年 | 353篇 |
1996年 | 344篇 |
1995年 | 299篇 |
1994年 | 245篇 |
1993年 | 187篇 |
1992年 | 191篇 |
1991年 | 161篇 |
1990年 | 161篇 |
1989年 | 114篇 |
1988年 | 121篇 |
1987年 | 104篇 |
1986年 | 96篇 |
1985年 | 150篇 |
1984年 | 139篇 |
1983年 | 88篇 |
1982年 | 122篇 |
1981年 | 90篇 |
1980年 | 79篇 |
1979年 | 55篇 |
1978年 | 34篇 |
1977年 | 45篇 |
1976年 | 30篇 |
1975年 | 26篇 |
Currently, multiple myeloma (MM) is an incurable disease. Despite the fact that arsenic trioxide (ATO) shows promising results in vitro, data from treatment of patients with MM are disappointing. Due to these discrepancies, we compared the efficacy and selectivity of ATO at two different concentrations in samples from MM patients.
Methods
The extent of apoptosis induced by 2 and 5 µM ATO was evaluated by flow cytometry using annexin V. 34 diagnostic bone marrow samples obtained from MM patients were analysed.
Results
5 µM ATO efficiently induced apoptosis in primary samples. Besides efficacy, also selectivity of action on MM cells in comparison to remaining haematopoietic cells was demonstrated for 5 µM ATO but not for 2 µM ATO.
Discussion
Our study on primary samples confirmed that ATO has a potential role in therapeutic management of MM. Further controlled studies on MM patients are needed. 相似文献
The presence of numerical and/or structural chromosomal abnormalities is a frequent finding in clonal hematopoietic malignant disease, typically diagnosed through routine karyotyping and/or fluorescent in situ hybridization (FISH) analysis. Recently, the application of array comparative genomic hybridization (aCGH) has uncovered many new cryptic genomic copy number imbalances, most of which are now recognized as clinically useful markers of haematological malignancies. In view of the limitations of both FISH and aCGH techniques, in terms of their routine application as a first line screening test, we designed a new multiple ligation-dependent probe amplification (MLPA) probemix for use in addition to classic karyotype analysis.
Methods
A novel MLPA probemix was developed to interrogate copy number changes involving chromosomal regions: 2p23-24 (MYCN, ALK), 5q32-34 (MIR145A, EBF1, MIR146A), 6q21-27, 7p12.2 (IKZF1), 7q21-36, 8q24.21 (MYC), 9p24 (JAK2 V617F point mutation), 9p21.3 (CDKN2A/2B), 9p13.2 (PAX5), 10q23 (PTEN), 11q22.3 (ATM), 12p13.2 (ETV6), 13q14 (RB1, MIR15A, DLEU2, DLEU1), 17p13.1 (TP53), and 21q22.1 (RUNX1/AML1) and was applied to DNA extracted from 313 consecutive bone marrow patient samples, referred for routine karyotype analysis.
Results
More than half of the samples originated from newly investigated patients. We discovered clinically relevant genomic aberrations, involving a total of 24 patients (8%) all with a normal karyotype, which would have remained undiagnosed.
Discussion
Our data clearly indicate that routine application of this MLPA screening panel, as an adjunct to karyotype analysis, provides a sensitive, robust, rapid and low-cost approach for uncovering clinically important genomic abnormalities, which would have otherwise remained undetected. 相似文献