对医院职工自身无形资产的探讨

简述了医院职工自身无形资产的内涵，指出自身无形资产既包含文凭、职称、技术等级证书等，又含有职业道德、情商、智商及学识水平等。强调医院职工在工作中要注意体现无形资产的有形价值，要学会珍惜和运用自身无形资产，不断提高劳动技能和创造才能，从而在激烈的岗位竞争中取胜。而作为医院管理者，要认识到无形资产的重要作用，引导职工走向人的全面自由发展。     

Sr action potentials in cardiac Purkyně fibres

Summary The characteristics of the regenerative increase in conductance for Sr ions in cardiac Purkyn fibres was examined as a function of (Sr) _e , in the presence and absence of Ca ions. The maximum rate of depolarization during the upstroke of the action potential was not linearly related to log (Sr) _e but tended to become constant at high external Sr concentrations, and was also inhibited by increasing Ca concentrations. The conductance for Sr ions as a function of (Sr) _e follows Michaëlis-Menten characteristics, suggesting that the Sr conductance is determined by a first order reaction between Sr and a site on the cell membrane. The behaviour in the presence of Ca can be explained by Ca acting as a competitive inhibitor.     

谈医院核心竞争力

未来医疗服务市场的竞争将是核心竞争力的竞争。面对国内外医疗市场一体化，我国医院今后的战略选择只能是大力培育核心竞争力。作者在对医院核心竞争力概念进行界定的基础上，分析了医院核心竞争力的特点及构成要素，提出了医院核心竞争力的构建措施。     

知难而进勇于实践

作者着重论述了山东省认真贯彻落实中央有关政策和李岚清副总理的讲话精神、全面推进医疗卫生体制改革的情况。在卫生行政部门职能转变方面、在推进区域卫生规划方面、在医疗机构分类管理工作方面、在计区卫生服务工作方面、在人事制度改革方面、在推进病人选医生制度方面、在医院后勤服务社会化改革方面、在完善补偿机制方面、在药品集中招标采购试点工作方面、在医院药品收支两条线管理方面取得了一定的进展。     

护生竞争与合作策略取向的研究与分析

目的 了解护生的竞争／合作策略取向及其相关因素，为护理教学改革提供参考。方法 采用问卷调查法，以“竞争／合作策略标度”量表作为测量工具，采用张智研制的相关性量表进行分析。结果 护生的竞争策略均分值高于合作策略均分值。护理本科生合作和竞争策略均分值的差异有显著性（P〈0．01）。结论 护理教学应注重加强素质教育，从改革教学方法和考评方式的角度，减轻竞争压力，培养护生合作意识，增强合作能力。     

Transient Receptor Potential Vanilloid 1 Expression and Functionality in MCF-7 Cells: A Preliminary Investigation

Purpose

Transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel belonging to the transient receptor potential family, and it is expressed in different neoplastic tissues. Its activation is associated with regulation of cancer growth and progression. The aim of this research was to study the expression and pharmacological characteristics of TRPV1 in cells derived from human breast cancer MCF-7 cells.

Methods

TRPV1 presence was assessed by binding studies and Western blotting. Receptor binding characteristics were evaluated through competition assays, while 3-(4,5-dimethylthiazol-2-yl)-2,5,-dipheyltetrazolium bromide reduction assays were performed to confirm an early hypothesis regarding the modulation of cancer cell proliferation. The functionality of TRPV1 was evaluated by measuring Ca²⁺ uptake in the presence of increasing concentrations of TRPV1 agonists and antagonists.

Results

Binding studies identified a single class of TRPV1 (B_max 1,492±192 fmol/mg protein), and Western blot showed a signal at 100 kDa corresponding to the molecular weight of human TRPV1. Among the different tested agonists and antagonists, anandamide (Ki: 2.8×10^-11 M) and 5-iodoresiniferatoxin (5-I-RTX) (Ki: 5.6×10^-11 M) showed the highest degrees of affinity for TRPV1, respectively. All tested TRPV1 agonists and antagonists caused a significant (p<0.05) decrease in cell growth rate in MCF-7 cells. For agonists and antagonists, the efficacy of tested compounds displayed the following rank order: resiniferatoxin>anandamide>capsaicin and 5-I-RTX=capsazepine, respectively.

Conclusion

These data indicate that both TRPV1 agonists and antagonists induce significant inhibition of MCF-7 cell growth. Even though the mechanisms involved in the antiproliferative effects of TRPV1 agonists and antagonists should be further investigated, it has been suggested that agonists cause desensitization of the receptor, leading to alteration in Ca²⁺-influx regulation. By contrast, antagonists cause a functional block of the receptor with consequent fatal dysregulation of cell homeostasis.     

Evaluation of the interspecific competitive ability of the bioincising fungus Physisporinus vitreus

A hierarchical set of assays were used to evaluate the interspecific competitive ability of the bioincising fungus Physiporinus vitreus (response species). The competitiveness and growth mode of P. vitreus against a range of blue stain fungi and Trichoderma species (challenge species) was investigated in dual culture tests (a) and a spatially heterogeneous system of tessellation agar (b) on different media. In addition, the robustness of the bioincising process against biotic influences was determined by controlled interaction tests with different inocula of the challenge species (colony-forming units: 10(2), 10(4), 10(6) ml(-1)) on Norway spruce (Picea abies Karst.) heartwood. The results of the dual culture and tessellation agar tests revealed a significant (P ≤ 0.001) antagonistic potential (lethal effect) of the T. atroviride strain (15603.1), whereas no or only a slight effect of the blue stain fungi on P. vitreus was detectable. The interaction tests on Norway spruce heartwood confirmed that an increase in inoculum size of the challenge fungi correlated with an increased negative effect (Spearman's rho coefficient (ρ) = -0.521). Among the challenge species, T-15603.1 revealed the most negative effect on the homogeneous development of P. vitreus on wood and thus on the outcome of the bioincising process. To improve the uniformity of colonization and to reduce the rate of contamination, the incubation of wood in a sealed system (bioreactor) after sterilization is suggested.     

我国胰岛素市场状况浅析

根据国际糖尿病联盟(IDF)统计,2011年全球糖尿病患者为3.66亿,近年来我国糖尿病发病率迅速上升的严峻形势令人震惊。目前治疗糖尿病的药物主要为口服制剂和胰岛素制剂,而胰岛素制剂则是最直接和最有效的治疗方法,且市场份额逐年上升。所以,文章对胰岛素制剂的分类、市场现状等进行研究与分析,以期对我国相关产业的发展提供一定的现实意义。     

提高寄生虫病技能竞赛成绩的体会

通过对参加的几次全国及省级寄生虫病防治技术竞赛进行回顾, 总结不断提升寄生虫病检测技能、 提高竞赛成 绩的体会。