沟通理论模式在神经外科失语病人中的运用研究

目的探讨沟通理论在神经外科失语病人中的运用价值。方法对我院神经外科收治的28例神志清楚的失语患者运用恰当语言交流、自制图片、自编手语暗号和提供写字板等形式与病人沟通。结果89％的病人表示能清楚地表达所需,对护理工作的满意达96％。结论良好的沟通不仅能减少病人紧张、烦躁的情绪,满足病人身心的需要,也有利于病情的康复。     

氯吡格雷在非ST段抬高心肌梗死患者急诊冠脉介入治疗中的作用

目的 观察氯吡格雷(clopidogrel)应用于非ST段抬高心肌梗死患者急诊介入治疗的作用.方法 选择诊断为非ST段抬高心肌梗死患者112例,随机分为对照组和氯吡格雷组,每组各56例,均实行相同的标准治疗.病例均急诊行冠脉造影及冠脉介入治疗(PCI).氯吡格雷组除标准治疗外,入院即刻给予氯吡格雷,对照组给予噻氯匹啶.2组均随访半年.结果 PCI术后氯吡格雷组患者的TIMI 3级血流获得数为91.1%,较对照组(87.5%)高,2组间差异无统计学意义(P>0.05).2组在心功能分级、威胁生命出血方面亦无统计学差异(P>0.05).氯吡格雷组轻度出血明显少于对照组(P<0.05),氯吡格雷组血小板降低程度、白细胞总数均明显低于对照组(P<0.05).半年的随访中,氯吡格雷组联合终点与对照组比较差异有统计学意义(P<0.05).结论 氯吡格雷对非ST段抬高心肌梗死患者急诊介入治疗有良好的效果,能降低急诊介入治疗的心血管事件风险.     

Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage

The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.     

幽门螺杆菌诱导胃上皮细胞表达分泌IL-8的研究

目的:探讨幽门螺杆菌(Helicobacter pylori,Hp)体外诱导胃上皮细胞表达分泌IL-8的状况.方法:逆转录多聚酶链反应(RT-PCR)检测IL-8在Hp诱导的胃上皮细胞的表达,酶联免疫吸附试验(ELISA)检测IL-8表达产物的量.结果:经Hp标准菌株CCUG17874刺激后,在胃上皮细胞株MKN 45、MKN 28、SGC 7901均检测到IL-8 mRNA;共同培养60 min 开始明显表达(P<0.05),120～180 min时表达最强(P<0.01).刺激24 h后, MKN 45分泌IL-8蛋白最高,为(1 480±360) pg/ml,SGC7901为(1 120±290) pg/ml, MKN 28最低,为(710±220) pg/ml,而未刺激对照组为(63±21 )、(52±16)和(30±18)pg/ml, 刺激组与各自未刺激对照组间有显著差异(P＜0.01),其相差倍数分别为23.5、 21.5、23.7倍,不同细胞株间的无统计学意义.结论:Hp能体外诱导胃上皮细胞株表达分泌IL-8,该作用可能与Hp感染后胃黏膜的炎症损伤机制有关.     

四逆汤对大鼠血清IgG水平的影响

本文以血清IgG水平为指标,研究了四逆汤及其各单味药附子、干姜、甘草对正常大鼠体液免疫的影响并对比附子、干姜、甘草三者的作用强度。结果表明:四逆汤全方和单味干姜、附子有显著提高正常大鼠血清免疫球蛋白G(IgG)水平,且干姜作用较附子强,而甘草显著降低正常,大鼠血清IgG。结果提示:四逆汤方药可提高正常大鼠体液免疫。     

Xiaotan Tongfu granules contribute to the prevention of stress ulcers

AIM:To investigate the efficacy and potential mechanism of Xiaotan Tongfu granules(XTTF)in stress ulcers.METHODS:One hundred sixty rats were randomly divided into 4 groups(n=10)as follows:the model group(MP group),the control group(CP group),the ranitidine group(RP group)and the XTTF granule group(XP group).Rats in the MP group received no drugs,rats in the CP group received 0.2 mL of a 0.9%sodium chloride solution via oral gavage,and rats in the RP and XP groups received the same volume of ranitidine(50 mg/kg)or XTTF granule(4.9 g/kg).The cold-restraint stress model was applied to induce stress ulcers after 7 consecutive days of drug administration.Afterwards,rats were sacrificed at 0,3,6 and24 h.Gastric pH was measured by a precise pH meter;gastric emptying rate(GER)was measured by using a methylcellulose test meal;myeloperoxidase activity(MPO),macrophage migration inhibitory factor(MIF),proliferating cell nuclear antigen(PCNA),and heat shock protein 70(HSP70)were measured by immunohistochemical staining;and mucosal cell apoptosis was measured by transferase dUTP nick end labeling.RESULTS:In the cold-restraint stress model,the development of stress ulcers peaked at 3 h and basically regressed after 24 h.Gastric lesions were significantly different in the RP and XP groups at each time point.Interestingly,although this index was much lower in the RP group than in the XP group immediately following stress induction(7.00±1.10 vs 10.00±1.79,P<0.05.Concerning gastric pH,between the RP and XP groups,we detected a statistically significant difference immediately after stress induction(0 h:4.56±0.47 vs 3.34±0.28,P<0.05)but not at any of the subsequent time points.For GER,compared to the RP group,GER was remarkably elevated in the XP group because a statistically significant difference was detected(3 h:46.84±2.70 vs 61.16±5.12,P<0.05;6 h:60.96±6.71 vs 73.41±6.16,P<0.05;24 h:77.47±3.17 vs 91.31±4.34,P<0.05).With respect to MPO and MIF,comparisons between the RP and XP groups revealed statisticall     

实验性马尾神经综合征家兔骶髓前角细胞凋亡的观察

目的：观察马尾神经综合征（CES）个体骶髓前角细胞的病理改变，以探讨其可能的发生机制。方法：选用纯种健康雄性新西兰兔80只，将压迫装置置于其S2－S3棘突之间压迫马尾神经制备CES模型。取出现CES后不同时期（发病后1／4，1／2，3，7，30d)的动物麻醉后，用生理盐水和4％多聚甲醛心脏灌注固定，取脊髓圆锥包埋，以5μm厚度连续切片，进行H－E染色和DNA片段原位末端标记（TUNEL）法检测，结合其形态学改变进行凋亡细胞计数，并作对比研究。结果：出现CES后1／ 4,TUNEL法检测示骶髓前角细胞发生凋亡改变；至1／2d时，凋亡细胞明显增多，且以早期凋亡细胞为主，与对照组比较有显著差异，H－E染色示骶髓前角细胞形态发生改变；以后凋亡细胞逐步减少，但仍高于对照组水平。结论：CES时骶髓前角细胞病理改变的主要方式是细胞凋亡；随着病情的发展，大部分凋亡细胞由凋亡早期进入死亡期（不可逆期），这可能是该病临床症状难以恢复的重要原因之一。     

HPLC梯度洗脱法测定山栀苷甲酯、8-0-乙酰山栀苷甲 酯在独一味中含量

目的 建立独一味中山栀苷甲酯、8-O-乙酰山栀苷甲酯的HPLC检测方法.方法 色谱柱Dikma platisil C18( 250 mm×4.6 mm,5 μm),柱温35℃,以乙腈-水进行梯度洗脱,流速1.0 mL/min,检测波长235 nm.结果 山栀苷甲酯进样量在0.023 0～3.450 0 μg范围内呈良好...     

Effect of cagE gene on Helicobacter pylori-associated gastritis and levels of interleukin-8

Helicobacterpylori (H .pylori)playsanimportantroleinthedevelopmentofchronicgastritis ,pepticulcer,gastricadenocarcinomaandgastricmucosa associatedlym phoidtissue (MALT)lymphoma .Mostpatientsremainla tentinfectionandonlysomedevelopintodiseasessuchaspepticulcerorgastriccancer[1,2 ] .Althoughwhatcausesaninfectionremainsunknown ,thereareseveralputativeviru lencefactorsthatmaycontributetothemucosaldamageandthenleadtovariousclinicaloutcomesbyH .pyloriinfec tion .cagpathogenicityisland (PAI)wascon…     

EGCG护肤品对女性面部皮肤的抗老化作用

目的评价含EGCG(epigallocatechin gallate)的护肤品对女性面部抗老化作用的临床效果和安全性。方法对33名健康女性受试者进行使用前后自身对照的临床研究。在全面部使用含EGCG的护肤品,试验为期8周。分别在0周,4周,8周检测面部皮肤的含水量、脱落角质细胞的数量以及进行皮肤纹理倒模成像。结果与0周(53.76±13.75)相比,皮肤的角质层含水量在4周(53.00±14.28)和8周(55.87±10.83)有轻度的升高,组间比较差异无显著性。皮肤脱落角质细胞的数量在8周时(9597.03±8442.07)与0周(57698.39±30038.87)相比下降83.4%,差异有显著性(P<0.001)。与皮肤皱纹和粗糙程度相关的参数从4周(0.88±0.14)开始得到改善,8周(0.78±0.15)时更为明显,差异有显著性(P<0.05)。试验过程中没有不良反应发生。结论含EGCG护肤品对皮肤早期老化所引起的皮肤干燥、角质细胞堆积、粗糙、细纹和皱纹有改善作用,并具有很好的安全性。