胃肠舒泰颗粒对大鼠胃泌素含量及小鼠胃肠运动功能的影响

目的 :观察胃肠舒泰颗粒对阿托品引起的小鼠胃排空及小肠推进功能抑制作用的影响 ,对正常大鼠血清胃泌素 (GAS)含量的影响。方法 :ICR小鼠 12 0只 ,胃排空实验、小肠推进实验各选 6 0只 ,分别随机分为 6组 ,即阴性对照组、模型对照组、胃肠舒泰颗粒小、中、大剂量组与普瑞博思组 ,以阿托品为抑制剂 ,观察各组胃排空及小肠推进率。SD大鼠 5 0只 ,分 5组 ,观察胃肠舒泰颗粒对大鼠血清 GAS含量的影响。结果 :胃肠舒泰颗粒可促进阿托品抑制的小鼠胃排空率及小肠推进率 ,并可增加正常大鼠血清 GAS的相对含量。结论 :胃肠舒泰颗粒对阿托品引起的小鼠胃排空及小肠推进功能抑制有良好的拮抗作用 ,同时具有升高动物血清 GAS含量的作用 ,这可能是其治疗胃肠动力障碍性疾病的药理学基础。     

Evaluation of biosynthesis parameters,stability and biological activities of silver nanoparticles synthesized by Cornus Officinalis extract under 365 nm UV radiation

Since silver nanoparticles (AgNPs) synthesized by using plant extracts revealed varied biological activities, the green synthesis of AgNPs has attracted considerable attention. Although the green synthesis of AgNPs have been accomplished by using the extracts of Cornus Officinalis, which is a traditional Chinese medicine and exhibits a wide spectrum of phytochemicals. The effects of biosynthesis parameters on reducing reaction, stability and more broad biological activities of so-prepared AgNPs did not been evaluated. In this paper, we firstly assessed the effects of UV radiation, pH, material proportion and radiation times on the green synthesis of AgNPs under 365 nm UV radiation by UV-visible spectrum and dynamic light scattering (DLS) analysis. The results showed that UV radiation could accelerate the formation of AgNPs and influence the average size below pH 7.0, and the size of so-prepared AgNPs were sensitive to the pH and material proportion, but no obvious changes to UV radiation times, offering a size-controlled synthetic method for AgNPs. The further X-ray diffraction (XRD), transmission electron microscopy (TEM) and DLS studies showed AgNPs synthesized at pH 7.0, extract: AgNO₃ = 1 : 1 and after 4 h UV radiation were a face-centered cubic (fcc) structure and both spherical and polygonal in shape with average particle size of 64.5 ± 0.3 nm existed in a monodispersed form. Subsquently, the stability of AgNPs was analyzed by zeta potential (−24.8 mV) and the average size measurement after 30 days storage (63.3 ± 0.4 nm), revealing a high degree of stability. Lastly, the investigation of biological activities showed that the biosynthesized AgNPs had potent antioxidant activity, antimicrobial activity against both S. aureus and E. coli as well as anticancer activity against HCT116 and HepG2 cell lines but negligible cytotoxicity against SW620. And the internalization of biosynthesized AgNPs inside the bacterial cell was evaluated by flow cytometric analysis, where the SSC values have significant increase after treating with nanoparticles. These results confirmed that the biosynthesis parameters on the green synthesis of AgNPs by using Cornus Officinalis extract also played pivotal roles and so-prepared AgNPs would be useful for the development of new alternative antioxidant, antimicrobial and anticancer agents in biomedicine.

We present herein a systematically evaluation of the biosynthesis parameters on the green synthesis of AgNPs by utilizing Cornus officinalis extract under 365 nm UV radiation.     

Effect of Zuogui pill and Yougui pill on osteoporosis: a randomized controlled trial

OBJECTIVE

To evaluate the effect and safety of Zuogui pill and Yougui pill, classic Yin and Yang tonic formula (CYYTF), in the treatment of osteoporosis and the underlying mechanism.

人工股骨头置换治疗骨质疏松性股骨粗隆间骨折36例

目的观察人工股骨头置换治疗骨质疏松性股骨粗隆间骨折的临床效果。方法对2008年1月—2013年6月收治的36例骨质疏松性股骨粗隆间骨折行加长柄双极骨水泥人工股骨头置换术,术后常规予鲑鱼降钙素联合钙剂抗骨质疏松治疗。结果术后Harris关节功能评分平均88分(68~94分),其中优18例,良13例,可4例,差1例,优良率为86.1%。2例术后活动后出现髋部轻度疼痛,1例下肢深静脉栓塞;本组均无髋关节脱位、假体周围再骨折、假体松动及下陷、大小粗隆骨折不愈合等并发症发生。结论人工股骨头置换术治疗骨质疏松性股骨粗隆间骨折可减少力学及内固定相关风险,且并发症少,效果良好。     

气范畴在中医理论建构中的方法论意义

以气范畴为基础的整体思维、取象思维、变易思维方式决定了中医学研究人体的基本路向，促进了中医理论的建构，是中医理论体系整体现、功能观、运动观特点形成的哲学基础。但气范畴的非结构特性，导致了对于具体事物间的联系往往是给予一种泛泛的哲学性的、方向性的说明，它在保持具象性的某些特点的同时，不自觉地限制了人们对认识的深入，在一定程度上又阻碍了中医学的发展。     

核因子-κB及钙超载与急性胰腺炎

急性胰腺炎(acute pancreatitis,AP)是一种常见的急腹症,主要继发于胆道疾病或慢性酒精中毒,大多数AP患者仅以胰腺出血、水肿为主要临床表现,但仍有25％的患者会发生急性坏死性胰腺炎,胰腺在出血、坏死的同时,常伴发全身炎症反应综合征(SIRS)和多器官功能障碍综合征(MODS),病死率可高达30％-40％[1]。针对AP的发病机制,学者们提出了多种学说,如胰酶消化学     

论天人合一观与《内经》理论的建构

天人合一的自然观从世界观、方法论、价值观等多方面促进了《内经》理论的建构，并使《内经》理论体系呈现出整体性、系统性、辩证性的特点，对未来医学的发展也有一定的启迪作用；同时分析了天人合一观的方法论缺陷，以及对中医学发展的阻碍作用。     

传统工艺与膜分离技术联合对马齿苋多糖的提取分离与抗氧化活性研究

目的将传统工艺与膜分离技术联用对马齿苋多糖进行分离纯化,并考察其体外抗氧化活性。方法采用L9(43)正交试验对马齿苋多糖的提取工艺进行优化;对马齿苋粗多糖脱蛋白和脱色素工艺进行优化;采用不同孔径的膜联用对马齿苋多糖进行精制研究;采用Fenton反应、邻苯三酚自氧化反应研究马齿苋多糖清除羟基自由基、超氧离子自由基活性。结果马齿苋多糖最佳提取条件为100℃,每次3 h,4次。Sevage法、三氯乙酸法和盐酸法脱蛋白多糖的回收率分别为17.05%、9.66%、16.61%。活性炭法、过氧化氢法、丙酮-无水乙醇法和氯仿-正丁醇法除色素多糖的回收率分别为17.04%、17.09%、27.06%、17.58%。膜分离研究表明纯化后的马齿苋多糖主要存在于0.8μm的渗透液中。马齿苋多糖对羟基自由基、超氧离子自由基的清除率分别为58.89%、38.89%。结论合适的微滤超滤膜联用可以达到纯化马齿苋多糖的目的。抗氧化活性研究显示马齿苋多糖能清除羟基自由基、超氧离子自由基且呈剂量依赖关系。     

贯叶连翘不同部位黄酮类成分差异与抗炎活性相关性分析

目的超高效液相色谱(UPLC)法结合模式识别方法分析贯叶连翘不同生长部位成分和抗炎活性的差异,建立同时定量测定贯叶连翘5个不同生长部位中6种黄酮类活性成分的方法。方法采用ACQUITY UPLC?BEH C18(50 mm×2.1mm,1.7μm)色谱柱,0.2%甲酸-水-乙腈作为梯度洗脱系统。采集贯叶连翘花、果、叶、茎、根5个不同部位样品的色谱信息,对积分数据进行中心化、归一化等预处理,采用偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)及PLS-tree聚类分析方法,以NO活性的半数抑制浓度(IC50)作为抗炎活性的监督因子,评价贯叶连翘不同部位在黄酮成分、抗炎活性方面的相似性和差异性。结果方法学验证结果显示,5种化合物线性关系良好,r0.999,精密度、重复性和稳定性良好,平均加样回收率为97.28%~102.84%。根据不同部位的黄酮类成分含量和抗炎活性PLS分析情况,显示贯叶连翘不同部位的质量为叶花茎果根。结论说明贯叶连翘采收部位应以地面上茎干部分开始采收为宜。     

头皮发际区微针疗法对血管性痴呆患者认知功能及载脂蛋白E的影响

目的：探讨头皮发际区微针疗法治疗血管性痴呆（VD）的疗效。方法：60例VD患者随机分为两组。观测组用头皮发际区微针疗法,对照组用口服药物疗法。用ELISA法捡测血浆中载脂蛋白E（ApoE）的浓度,采用MMSE和ADL量表评定患者的认知功能、社会活动功能及日常生活能力。结果：两组治疗效果比较：观测组和对照组总有效率分别为60.00%和53.33%,两组间有显著性差异（P〈0.05）;治疗后,观测组和对照组MMSE、ADL量表评分与治疗前自身对照均有明显改善（P〈0.01）;观测组治疗后的MMSE、ADL量表评分较对照组变化更为显著（P〈0.05）;两组血浆ApoE水平治疗后较治疗前自身比较均有显著下降（P〈0.01）;治疗后,观测组血浆ApoE水平与对照组比较,下降更为明显（P〈0.05）。结论：头皮发际区微针疗法对于VD患者具有确切疗效,其治疗机理与降低血浆ApoE含量相关。