生精汤对乳腺癌MCF-7细胞增殖影响的实验研究

目的观察生精汤对雌激素依赖性乳腺癌MCF-7细胞增殖的影响，揭示生精汤的雌激素活性。方法选用乳腺癌MCF-7细胞株，应用细胞增殖实验（E—SCREEN法）来检测生精汤的雌激素作用。实验可分为空白对照组与雌激素对照组（己烯雌酚，30tzg／mL）和生精汤低、中、高剂量组（分别为500、800、1000μg／mL），观察给药前后的细胞形态学变化，并运用四唑盐比色法（MTT法）测定乳腺癌MCF-7细胞的增殖率。结果生精汤高、中、低剂量组均可明显提高乳腺癌MCF-7细胞的增殖率，并呈现一定的量效关系。结论生精汤能显著提高乳腺癌MCF-7细胞的增殖率，即具有明显的雌激素样作用。     

加味二陈汤对ApoE-/-小鼠脂质代谢及肝细胞形态的影响

目的观察加味二陈汤对Apo E-/-小鼠血脂的影响。方法 44只Apo E-/-小鼠以高脂饲喂养4周构建AS小鼠模型,分别用辛伐他汀、加味二陈汤对小鼠灌胃给药,8周后处死小鼠,酶法检测血清血脂;每周称取并记录小鼠体重;油红O染色观察小鼠主动脉管腔内粥样斑块;HE染色观察小鼠主动脉管腔内粥样斑块沉积及肝细胞形态。结果加味二陈汤可显著降低小鼠血清中TCHOL、LDL-C水平(P<0.01);各组小鼠体重均有所增加,但模型组小鼠体重略高于空白对照组和加味二陈汤组;油红O染色结果显示加味二陈汤可显著减少Apo E-/-小鼠主动脉管腔内粥样斑块的面积;小鼠主动脉HE染色示加味二陈汤组小鼠主动脉内有脂质沉积斑块较模型组明显减少;肝细胞切片HE染色发现加味二陈汤组小鼠肝细胞的病变程度较轻,肝小叶结构较清晰。结论加味二陈汤能有效降低Apo E-/-小鼠血清中有关脂质的含量,调节小鼠脂质代谢,缓解动脉粥样硬化进程。     

中药联合激光光凝治疗糖尿病视网膜病变

糖尿病视网膜病变(diabetic retinopathy,DR)是糖尿病眼病不可逆盲的最严重的并发症,严重影响患者的生存质量。目前,我国在DR手术治疗方面已进行了广泛的开展,对于增殖前期DR,增殖期糖尿病视网膜病变(proliferative DR,PDR)采取全视网膜光凝术,破坏缺血区视网膜,减少需氧量,以防止新生血管形成,阻止病情恶化。中医药对治疗DR有着非常丰富及个性化的治疗方案,本文就中药联合激光光凝治疗DR的研究进行综述。     

RhoA/ROCK信号通路与动脉粥样硬化关系的研究进展

动脉粥样硬化是一种常见的全身性血管疾病,同时也是多种急性心血管疾病的病理基础。而急性心血管疾病的死亡率高,严重危害人们的生命健康。近年来,越来越多的研究表明RhoA/ROCK信号通路与动脉粥样硬化的形成有密切联系。抑制RhoA/ROCK信号通路可以通过稳定血管内皮功能、抑制血管平滑肌细胞增殖和迁移、抑制血管钙化、调控炎症细胞聚集以及抑制血小板增殖变形来延缓或抑制动脉粥样硬化的形成。     

姜黄素下调IDOL水平促进肝细胞摄取血浆LDLC

目的明确姜黄素可通过下调低密度脂蛋白受体诱导降解蛋白(IDOL)来升高低密度脂蛋白受体(LDLR)的表达水平,从而促进肝细胞摄取低密度脂蛋白胆固醇(LDLC)。方法利用过表达IDOL和干扰IDOL慢病毒(OE/RNAi-IDOL)感染HepG2和LO2两种体外培养的肝细胞,倒置荧光显微镜观察病毒感染情况;Western blot检测IDOL及LDLR蛋白表达;油红O染色观察细胞中脂质情况;酶法检测细胞内胆固醇含量;DiI-LDL摄取实验检测肝细胞对LDLC的摄取能力;流式细胞术检测肝细胞膜LDLR分布丰度。结果与明场比较,暗场下可见胞内呈绿色荧光;与对照组比较,三种不同干扰IDOL慢病毒感染的HepG2和LO2细胞中,仅RNAi-IDOL-2的IDOL蛋白表达显著降低,且相应组的LDLR蛋白表达显著增强(P0.01),选择为后续RNAi-IDOL组所用;相反,OE-IDOL慢病毒感染的HepG2和LO2细胞中,IDOL表达显著增高,相应组内LDLR表达减弱;以上结果表明已获得RNAi-IDOL-2及OE-IDOL慢病毒感染的HepG2和LO2细胞模型。与无药物处理的Control组相比,RNAi-IDOL-2及OE-IDOL慢病毒感染的肝细胞内经25μmol/L姜黄素处理24 h后,油红O染色及胆固醇含量测定结果显示:细胞内脂滴含量和相对胆固醇含量均显著增加(P0.01);且DiI-LDL摄取及免疫流式细胞结果显示:肝细胞摄取LDLC的能力增强(P0.01);肝细胞膜表面LDLR分布丰度增多(P0.01);以上结果与阳性药物对照组的结果趋势一致。结论姜黄素可以下调肝细胞内IDOL水平,提高细胞膜LDLR分布丰度,从而促进肝细胞摄取LDLC。     

Exosomal microRNA-25 released from cancer cells targets SIK1 to promote hepatocellular carcinoma tumorigenesis

BackgroundHepatocellular carcinoma (HCC) is recognized as a leading cause of cancer-associated fatality worldwide. Our study here aimed to probe the mechanism by which exosomes secreted by CSQT-2, an HCC cell line, affected the progression of HCC.MethodsExosomes were extracted from CSQT-2 cells. Colony formation, Transwell, sphere formation and flow cytometric analyses were applied to assess cell biological activities. Microarray analysis detected the change of microRNA (miRNA) expression after exosome treatment, followed by RT-qPCR validation. Luciferase reporter was applied to detect the binding between SIK1 and miR-25. Xenograft studies in nude mice manifested tumor growth and metastatic ability of miR-25 and SIK1.ResultsThe exosome treatment enhanced cell malignant phenotype in vitro and tumor growth and liver and lung metastases in vivo. The exosomes elevated miR-25 expression in HCC cells. miR-25 targeted SIK1 which was decreased in the exosomes-treated cells. miR-25 inhibitor reduced cell malignant phenotype and attenuated tumorigenesis and metastasis in vivo. SIK1 silencing reversed the effect of miR-25 inhibitor. The exosome treatment potentiated the Wnt/β-catenin pathway in cells, whereas miR-25 inhibitor blunted the pathway activity.ConclusionMiR-25 shuttled through CSQT-2-derived exosomes promoted the development of HCC by reducing SIK1 expression and potentiating the Wnt/β-catenin pathway.     

CKLF1/CCR5 axis is involved in neutrophils migration of rats with transient cerebral ischemia

BackgroundChemokine-like factor 1 (CKLF1) is a chemokine increased significantly in ischemic brain poststroke. It shows chemotaxis effects on various immune cells, but the mechanisms of CKLF1 migrating neutrophils are poorly understood. Recent studies have provided evidence that CC chemokine receptor 5 (CCR5), a receptor of CKLF1, is involved in ischemic stroke.PurposesTo investigate the effects of HIF-1α guided AAV in ischemic brain, investigating the outcome of stroke, and examining the involvement of CKLF1/CCR5 axis in recruitment of neutrophils.ResultsHIF-1α guided AAV knocked down CKLF1 in ischemic area and alleviated brain damage of rats. CKLF1 migrated neutrophils through CCR5, worsening inflammatory responses. Akt/GSK-3β pathway may involve in CKLF1/CCR5 axis guided neutrophils chemotaxis.ConclusionsCKLF1/CCR5 axis is involved in neutrophils migration of rats with transient cerebral ischemia. CKLF1/CCR5 axis may be a useful target for stroke therapy.     

Current research status of blood biomarkers in Alzheimer’s disease: Diagnosis and prognosis

Alzheimer’s disease (AD), which mainly occurs in the elderly, is a neurodegenerative disease with a hidden onset, which leads to progressive cognitive and behavioral changes. The annually increasing prevalence rate and number of patients with AD exert great pressure on the society. No effective disease-modifying drug treatments are available; thus, there is no cure yet. The disease progression can only be delayed through early detection and drug assistance. Therefore, the importance of exploring associated biomarkers for the early diagnosis and prediction of the disease progress is highlighted. The National Institute on Aging— Alzheimer’s Association (NIA-AA) proposed A/T/N diagnostic criteria in 2018, including Aβ42, p-tau, t-tau in cerebrospinal fluid (CSF), and positron emission tomography (PET). However, the invasiveness of lumbar puncture for CSF assessment and non-popularity of PET have prompted researchers to look for minimally invasive, easy to collect, and cost-effective biomarkers. Therefore, studies have largely focused on some novel molecules in the peripheral blood. This is an emerging research field, facing many obstacles and challenges while achieving some promising results.     

大学生完美主义对抑郁症状的预测研究

目的:在完美主义的双重过程模型的理论框架下,考察不同完美主义维度对抑郁的预测作用.方法:采用方便取样,选取湖南省某高校的在校大学生206名,每位参加者被要求先后参加两次测试,两次测试时间间隔4个月.第—次测试选用Frost多维完美主义量表(FMPS)、Hewitt多维完美主义量表(HMPS)、近乎完美主义量表(APS-R)、贝克抑郁问卷(BDI)、Rosenberg自尊量表(SES)、自我效能感量表(GSES)、状态焦虑问卷(S-AI)和正性负性情绪量表(PANAS).第二次测试选用贝克抑郁问卷(BDI).结果:偏相关分析显示,适应不良完美主义与心理症状学指标显著正相关(Pr=0.36,0.46,0.58;P＜0.001),而适应性完美主义与积极心理指标显著正相关(Pr=0.22,0.32,0.33;P＜0.01).横断面研究显示,适应不良完美主义中的差异性、社会决定完美主义因子能显著正向预测抑郁症状(β=0.372,P＜0.001;β=0.264,P＜0.01);适应性完美主义各因子均不能显著预测抑郁症状(P＞0.05).纵向研究结果显示,完美主义各因子均不能显著地预测4个月后抑郁水平的变化(P＞0.05).结论:本研究支持了完美主义的双重过程模型.适应不良完美主义中的差异性、社会决定完美主义因子能显著的正向预测抑郁水平.     

“肠病治肝”的理论探析与临床拾萃

"肝与大肠别通"理论首见于李梃的《医学入门》,其区别于"胆属肝"、"大肠属肺"表里脏腑络属关系,揭示脏腑之间另一种联系,故称别通。中医认为肝与大肠生理上都与水谷精微的运化输布、气机升降、腑气传导等功能相关,足厥阴肝经与手阳明大肠经之间存在间接联系。故其病理可相互影响,气机疏泄失常,肠腑传导失职则出现腹泻或便秘或大便溏结不调;肝不藏血,影响肠道血络,故可见便血等病理变化。近代医家杨维杰[1]提出脏腑气化相通为"脏腑别通"理论的实质。而随着现代医学进步,西医亦发现肝与肠可相互影响,提出"肠-肝轴"、"肝肠循环"等概念,为"肝与大肠别通"理论提供解剖生理、微观生物学上的解释。本文从肝与大肠生理、病理及经脉络属等方面探析其相关性,并以"肝与大肠别通"理论为指导思想浅析调肝经治疗肠腑病在针灸临床治疗中的运用,丰富针灸治疗肠腑病的思路。