生精汤对乳腺癌MCF-7细胞增殖影响的实验研究 |
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引用本文: | 徐攀,夏伯候,陈京.生精汤对乳腺癌MCF-7细胞增殖影响的实验研究[J].中国现代医生,2013,51(17):129-131. |
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作者姓名: | 徐攀 夏伯候 陈京 |
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作者单位: | 徐攀 (浙江省中医药研究院蚕业资源研究中心,浙江杭州,310007); 夏伯候 (湖南中医药大学药学院,湖南长沙,410208); 陈京 (浙江中医药大学生物工程学院,浙江杭州,310053); |
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基金项目: | 浙江省中医药科技计划资助项目(项目编号:2009CA004) |
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摘 要: | 目的观察生精汤对雌激素依赖性乳腺癌MCF-7细胞增殖的影响,揭示生精汤的雌激素活性。方法选用乳腺癌MCF-7细胞株,应用细胞增殖实验(E—SCREEN法)来检测生精汤的雌激素作用。实验可分为空白对照组与雌激素对照组(己烯雌酚,30tzg/mL)和生精汤低、中、高剂量组(分别为500、800、1000μg/mL),观察给药前后的细胞形态学变化,并运用四唑盐比色法(MTT法)测定乳腺癌MCF-7细胞的增殖率。结果生精汤高、中、低剂量组均可明显提高乳腺癌MCF-7细胞的增殖率,并呈现一定的量效关系。结论生精汤能显著提高乳腺癌MCF-7细胞的增殖率,即具有明显的雌激素样作用。
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关 键 词: | 乳腺癌MCF-7细胞 生精汤 细胞增殖 |
The study on effects of Shengjing decoction on the activity of breast can- cer cell line MCF-7 |
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Authors: | XU PanI XIA Bohou CHEN Jing |
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Institution: | 1.Zhejiang Academy of Traditional Chinese Medicine, Exploit and Research Center of Officinal Silkworm Resources, Hangzhou 310007, China ; 2.Hunan Chinese Medical University, College of Pharmacy, Changsha 410208, China ; 3.Zhejiang Chinese Medical University,College of Bioengineering, Hangzhou 310053,China |
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Abstract: | Objective To observe the estrogenic activity of Shengjing decoction. Methods The MCF-7 human breast cancer was applied to observe proliferation rate by different concentrations of Shengjing decoction, and the prolifera- tion rate of cells was detected by MTT. It was divided into 5 groups: control group, estrogen group (diethylstilbestrol, 30 μg/mL) and Shengjing Decoction groups with low, medium and high dosage (500,800, 1000 ptg/mL). Results Shengjing decoction could significantly increase the proliferation rate of MCF-7 human breast cancer dose-depen- dently. And the proliferation rate of Shengjing decoction group with high dosage was similar to that of estrogen group. Conclusion Shengjing decoction could increased breast cancer MCF-7 cell's proliferation effectively. |
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Keywords: | Breast cancer line MCF-7 Shengjing decoction Cell proliferation |
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